• 제목/요약/키워드: BioInformatics

검색결과 580건 처리시간 0.025초

2'-Hydroxylation of Genistein Enhanced Antioxidant and Antiproliferative Activities in MCF-7 Human Breast Cancer Cells

  • Choi, Jung-Nam;Kim, Doc-Kyu;Choi, Hyung-Kyoon;Yoo, Kyung-Mi;Kim, Ji-Young;Lee, Choong-Hwan
    • Journal of Microbiology and Biotechnology
    • /
    • 제19권11호
    • /
    • pp.1348-1354
    • /
    • 2009

  • Bioconversion of the isoflavonoid genistein to 2'-hydroxygenistein (2'-HG) was performed using isoflavone 2'-hydroxylase (CYP81E1) heterologously expressed in yeast. A monohydroxylated product was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and NMR spectrometry and was identified as 2'-HG. An initial bioconversion rate of 6% was increased up to 14% under optimized conditions. After recovery, the biological activity of 2'-HG was evaluated. Bioconverted 2'-HG showed higher antioxidant activity against 1,1-diphenyl-2-picryl hydrazine (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals than did genistein. Furthermore, 2'-HG exhibited greater antiproliferative effects in MCF-7 human breast cancer cells than did genistein. These results suggest that 2'-hydroxylation of genistein enhanced its antioxidant activity and cell cytotoxicity in MCF-7 human breast cancer cells.

  • https://doi.org/10.4014/jmb.0903.0114 인용 PDF KSCI

Bio-Optical Modeling of Laguna de Bay Waters and Applications to Lake Monitoring Using ASTER Data

  • Paringit, EC.;Nadaoka, K.;Rubio, MCD;Tamura, H.;Blanco, Ariel C.
    • 대한원격탐사학회:학술대회논문집
    • /
    • 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
    • /
    • pp.667-669
    • /
    • 2003

  • A bio-optical model was developed specific for turbid and shallow waters. Special studies were carried out to estimate absorption and scattering properties as well as backscattering probability of suspended matter. The inversion of bio-optical model allows for direct retrieval of turbidity and chlorophyll- a from the visible-near infrared (VNIR) range sensor. Time-series satellite imagery from ASTER AM-1 sensor, were used to monitor the Laguna de Bay water quality condition. Spatial distribution of temperature for the lake was extracted from the thermal infrared (TIR) sensor. Corresponding field surveys were conducted to parameterize the bio -optical model. In-situ measurements include suspended particle and chlorophyll-a concentrations profiles from nephelometric devices and processing of water samples. Hyperspectral measurements were used to validate results of the bio -optical model and satellite- based estimation. This study provides a theoretical basis and a practical illustration of applying space- based measurements on an operational basis.

  • PDF

Genome-Wide Association Study of Metabolic Syndrome in Koreans

  • Jeong, Seok Won;Chung, Myungguen;Park, Soo-Jung;Cho, Seong Beom;Hong, Kyung-Won
    • Genomics & Informatics
    • /
    • 제12권4호
    • /
    • pp.187-194
    • /
    • 2014

  • Metabolic syndrome (METS) is a disorder of energy utilization and storage and increases the risk of developing cardiovascular disease and diabetes. To identify the genetic risk factors of METS, we carried out a genome-wide association study (GWAS) for 2,657 cases and 5,917 controls in Korean populations. As a result, we could identify 2 single nucleotide polymorphisms (SNPs) with genome-wide significance level p-values (< $5{\times}10^{-8}$), 8 SNPs with genome-wide suggestive p-values ($5{\times}10^{-8}{\leq}$ p < $1{\times}10^{-5}$), and 2 SNPs of more functional variants with borderline p-values ($5{\times}10^{-5}{\leq}$ p < $1{\times}10^{-4}$). On the other hand, the multiple correction criteria of conventional GWASs exclude false-positive loci, but simultaneously, they discard many true-positive loci. To reconsider the discarded true-positive loci, we attempted to include the functional variants (nonsynonymous SNPs [nsSNPs] and expression quantitative trait loci [eQTL]) among the top 5,000 SNPs based on the proportion of phenotypic variance explained by genotypic variance. In total, 159 eQTLs and 18 nsSNPs were presented in the top 5,000 SNPs. Although they should be replicated in other independent populations, 6 eQTLs and 2 nsSNP loci were located in the molecular pathways of LPL, APOA5, and CHRM2, which were the significant or suggestive loci in the METS GWAS. Conclusively, our approach using the conventional GWAS, reconsidering functional variants and pathway-based interpretation, suggests a useful method to understand the GWAS results of complex traits and can be expanded in other genomewide association studies.

  • https://doi.org/10.5808/GI.2014.12.4.187 인용 PDF KSCI

Informatics for protein identification by tandem mass spectrometry; Focused on two most-widely applied algorithms, Mascot and SEQUEST

  • Sohn, Chang-Ho;Jung, Jin-Woo;Kang, Gum-Yong;Kim, Kwang-Pyo
    • Bioinformatics and Biosystems
    • /
    • 제1권2호
    • /
    • pp.89-94
    • /
    • 2006

  • Mass spectrometry (MS) is widely applied for high throughput proteomics analysis. When large-scale proteome analysis experiments are performed, it generates massive amount of data. To search these proteomics data against protein databases, fully automated database search algorithms, such as Mascot and SEQUEST are routinely employed. At present, it is critical to reduce false positives and false negatives during such analysis. In this review we have focused on aspects of automated protein identification using tandem mass spectrometry (MS/MS) spectra and validation of the protein identifications of two most common automated protein identification algorithms Mascot and SEQUEST.

  • PDF