• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1475-1479
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• 2012

Human peroxisome proliferator-activated receptor gamma ($hPPAR{\gamma}$) has been implicated in numerous pathologies, including obesity, diabetes, and cancer. In this study, we verified that amentoflavone is an agonist of $hPPAR{\gamma}$ and probed the molecular basis of its action. It was demonstrated that amentoflavone bound $hPPAR{\gamma}$ with high (picomolar) affinity and increased the binding between $hPPAR{\gamma}$ and steroid receptor coactivator-1 (SRC-1) by approximately 4-fold. Based on a docking study, for the first time, we propose a model of amentoflavone and $hPPAR{\gamma}$ binding in which amentoflavone forms three hydrogen bonds with the side chains of His323, Tyr327, and Arg280 in $hPPAR{\gamma}$ and participates in two hydrophobic interactions.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1607-1615
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• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• 한국초전도학회:학술대회논문집
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• v.16
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• pp.9-9
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• 2006

• 한국초전도학회:학술대회논문집
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• v.16
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• pp.55-55
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• 2006

• 한국초전도학회:학술대회논문집
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• 2007.07a
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• pp.50-50
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• 2007

• 한국초전도학회:학술대회논문집
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• 2007.07a
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• pp.61-61
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• 2007

• 한국초전도학회:학술대회논문집
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• 2008.08a
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• pp.54-54
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• 2008

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.53.1-53.1
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• 2008

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.52.2-52.2
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• 2008

• Journal of Environmental Health Sciences
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• v.41 no.5
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• pp.327-334
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• 2015

Objectives: In order to review both the effects of sewage sludge dewaterability and deodorizing ability in the existing advanced sewage treatment process, BIO-CLOD (a solidified mixture of 10% Bacillus concentrate and inorganics) was used as an inorganic conditioner effective for deodorization treatment in addition to the removal of organic matter and nutritive salts. Methods: Sludge dewaterability was evaluated using specific resistance to filtration (SRF) experiments with six agitators and two types of commercially available BIO-CLOD; one that is made by grinding solid matter (powdered BIO-CLOD) and one that has been obtained by sieving a separation with a particle diameter of 100 mesh (100 mesh BIO-CLOD). For deodorization odor treatment experiments, 20 g of commercially available solid BIO-CLOD was submerged in four liters of sewage sludge and mixed and agitated for 45 days. Results: When BIO-CLOD was injected into sewage sludge to experimentally compare specific resistance to filtration (SRF), the optimum amount of BIO-CLOD to be injected was shown to be 2% w/v and the SRF value in this case was $1.35{\times}10^{12}m/kg$. pH changes following BIO-CLOD injection were within 6.5-7.0. By 14 days after submerging BIO-CLOD into the sewage sludge to evaluate its deodorizing ability, $H_2S$ decreased by 68% and methyl mercaptan decreased by 74%. By 45 days after the submergal, both items decreased by 100%, indicating deodorizing ability. Conclusion: To compare the levels of dewaterablity of sewage sludge at different particle sizes of inorganic conditioner, powdered BIO-CLOD, particle size 100 mesh BIO-CLOD, and bentonite were tested. It could be seen that as the powdered BIO-CLOD increased, the precipitability increased up to 62% in 30 minutes. As an inorganic conditioner, BIO-CLOD was identified as a stable sludge conditioner that does not affect pH.