• Korean Journal of Organic Agriculture
• /
• v.7 no.2
• /
• pp.107-114
• /
• 1999

This study was carried out to investigate the effect of microbial fertilizers on yields of Chinese cabbage(Brassica campestris L.). Six microbial fertilizers, MPK+Husk+Palma, Husk+Palma, MPK+Compost, Compost, Bio livestock cattle system(BLCS) cattle dropping, and Tomi, were used. All of the microbial fertilizers were very effective to increase yields of Chinese cabbage. As a result of analysis of plant chemical components, MPK+Compost treated plot showed higher concentrations of Fe and Zn than other microbial fertilizers plot. In a chemical components of soil, concentrations of K, Mg were increased with Tomi treatment, however, the other concentrations of plant and soil chemical components were not different. In a microbial properties of soil, Tomi, Husk+Palma, and MPK+Husk+Palma treated plots increased in numbers of total bacteria and bacillus. Tomi treated plot increased in numbers of actinomycetes and fungi, also. The other microbial properties of soil were not different, however. The microbial fertilizers may affect the useful microbial properties, therefore, it would be increase yield of Chinese cabbage treated with them.

• Korean Journal of Organic Agriculture
• /
• v.19 no.spc
• /
• pp.119-122
• /
• 2011

Among the developed bio-degradable polymer films as compared with transparent film(White), black polymer film was significantly more effective for controlling weeds in red pepper. Also, we found that white and black polymer mulching had 81.8% and 97.9% of managing weed controlling effects in rice, respectively. Compared to non-mulched rice paddy with water supply, the non-mulched rice paddy without any water supply has stopped its growth at 41 days after transplanting, while polymer-mulched rice paddy without water supply had about 60% of normally growing rice plants. This shows the polymer treatment has a remarkable effect on water and power saving, solution of herbicidal resistance, avoidance of herbicidal influence to eco-system etc. When the naturally decomposing polymer was used, a temperature was elevated as high as $4.7^{\circ}C$ on maximum and $2.6^{\circ}C$ on average. Also the naturally decomposing polymer accelerated rooting by 7 days and lowered a stress level from transplanting. The weed control effect mulched by polymer was remarkable as 98.7%. The polymer now, after 294 days treated on the rice paddy, has been completely decomposed.

• Korean Journal of Food Science and Technology
• /
• v.32 no.5
• /
• pp.997-1001
• /
• 2000

The organic acids in 3 kinds of medicinal herbal extracts were screened and compared each other according to their organic acid contents by an efficient gas chromatographic method. It involves solid-phase extraction of organic acids using Chromosorb P with subsequent conversion to stable tert-butyldimethysilyl derivatives for the direct analysis by capillary column gas chromatography and gas chromatography-mass spectrometry. Total of 24 organic acids were reproducibly identified from 3 kinds of herbal extracts. When the GC profiles were simplified to their retention index spectra, characteristic patterns were obtained for each herb sample. As expected, three kinds of herbal extracts showed three distinct patterns.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.5
• /
• pp.2671-2677
• /
• 2014

These days, there is increasing those who like spicy food, people release stress by eating spicy food. But, when you eat spicy food, there is a difference but, we visually can be found red facial color to change. In this paper, when you eat spicy food, we carried out experiment which comparison and analysis color change of facial area depending on Sasang constitutional type. To this end, we organized test subject group by Sasang constitutional type according to survey result for Sasang constitutional type. And then we carried out interrelationship analysis between spicy food and facial color depending on facial color to apply Lab color system based on facial image which is before and after eat a hot spicy pepper.

• Journal of the Korean Magnetic Resonance Society
• /
• v.16 no.2
• /
• pp.91-102
• /
• 2012

Metabolomics is the systematic identification and quantification of all metabolites in an organism or biological sample. NMR has been used as a major application tool in plant metabolomics such as quality control, chemotaxonomy, and analysis of genetically modified plants. Herbal medicines are the important therapeutics and are used to manage common diseases such as cold, inflammation, pain, heart diseases, liver cirrhosis, diabetes and central nerve system diseases. Herb plants include various kinds of species such as geranium, mint, and thyme and so on and contain different kinds of metabolites. We performed NMR-based metabolomics study on the seven different species of herb plants using $^1H$ NMR experiments and OPLS-DA to understand the correlation between the classification of herb plants and their metabolite contents. This study showed clear metabolic discrimination among various herb plants. This metabolmics study found several diagnostic NMR signals which are able to be used as bio-markers for identification of the specific herb plants among various species. Clear metabolic discrimination of herb plants suggests three chemotaxonomic groups of herb species.

• Journal of Radiation Industry
• /
• v.9 no.4
• /
• pp.199-207
• /
• 2015

Typical radiation cross-linked hydrogels has the characteristic that high water content, but low emission efficiency of active ingredients. Therefore, the hydrogel was prepared by the addition to collagen, which is closely related to the formation of skin wrinkles in biocompatibility and highly water-soluble carboxymethyl cellulose sodium salt (CMC) in order to preparation of hydrogels has excellent emission efficiency of active ingredients. Hydrogels were prepared by dissolving CMC and collagen each of 0.5%, 10% concentration in deionized water. Then, prepared hydrogels are performed by gamma-radiation at 1, 3, 5 kGy irradiation dose. The results showed that the gel fraction of after irradiated 3 kGy hydrogel was higher than before irradiated gelation as long as the 55.3%. The swelling rate of irradiated 3 kGy hydrogel was lower than the non-irradiated sample. The compressive strength of 3 kGy irradiated hydrogel was the highest. The visco-elastic did not show any significant differences, even after irradiation. The CMC hydrogel in this study suggested a potential use as a material for the mask pack for improved emission efficiency of the active ingredient and anti-wrinkles.

• Development and Reproduction
• /
• v.23 no.2
• /
• pp.139-148
• /
• 2019

Rising of water temperature due to global warming is a great concern to aquaculturists and fishery biologists. Hence, the present study aimed to investigate the effects of high water temperature on juvenile red spotted grouper, Epinephelus akaara based on the evaluation of stress responses in blood. E. akaara juveniles were exposed to different thermal conditions ($25^{\circ}C$, $28^{\circ}C$, $31^{\circ}C$, and $34^{\circ}C$) for 6 weeks following 2 weeks of acclimation at $25^{\circ}C$. Blood cell morphology and number were examined at three sampling points (2, 7, and 42 days) from a total of 180 fish. Major erythrocytic cellular abnormalities (ECA) observed in blood smears of thermally stressed groups ($31^{\circ}C$ and $34^{\circ}C$) after 6 weeks were echinocytes, teardrop-like cells, swollen cells and vacuolated cells. Both red and white blood cell number (RBC and WBC) were significantly (p<0.05) elevated in $31^{\circ}C$ and $34^{\circ}C$ group after 6 weeks thermal exposure. Differential leucocytes number showed significant increases in neutrophil (N) and decreases in lymphocytes (L) in the highest temperature ($34^{\circ}C$). Different N:L ratio was observed at different thermal conditions which can be used as a reliable alternative to measure stress response. Taken together, these results suggest that higher temperature ($31^{\circ}C$ and $34^{\circ}C$) can interfere the immune system of red spotted grouper by altering the blood cell morphology and number.

• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.11
• /
• pp.1789-1800
• /
• 2019

Objective: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. Methods: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. Results: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. Conclusion: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.

• Advances in nano research
• /
• v.4 no.1
• /
• pp.15-29
• /
• 2016

A low cost environmentally benign surface coating binder is highly desirable in the field of material science. In this report, castor oil based hyperbranched polyester/bitumen modified fly ash nanocomposites were fabricated to achieve the desired performance. The hyperbranched polyester resin was synthesized by a three-step one pot condensation reaction using monoglyceride of castor oil based carboxyl terminated pre-polymer and 2,2-bis (hydroxymethyl) propionic acid. Also, the bulk fly ash of paper industry waste was converted to hydrophilic nano fly ash by ultrasonication followed by transforming it to an organonano fly ash by the modification with bitumen. The synthesized polyester resin and its nanocomposites were characterized by different analytical and spectroscopic tools. The nanocomposite obtained in presence of 20 wt% styrene (with respect to polyester) was found to be more homogeneous and stable compared to nanocomposite without styrene. The performance in terms of tensile strength, impact resistance, scratch hardness, chemical resistance and thermal stability was found to be improved significantly after formation of nanocomposite compared to the pristine system after curing with bisphenol-A based epoxy and poly(amido amine). The overall results of transmission electron microscopic (TEM) analysis and performance showed good exfoliation of the nano fly ash in the polyester matrix. Thus the studied nanocomposites would open up a new avenue on development of low cost high performing surface coating materials.

• The Journal of Traditional Korean Medicine
• /
• v.15 no.1
• /
• pp.49-55
• /
• 2006

Objective: <千金方(Qianjinfang)> which documents a great amount of prescription of Acupuncture and moxibustion bikes an important role during the development of acupuncture and moxibustion. Thus, it have given rise us to the research-situation of Tang Dynasty China of previous period. This study was to outline the rules of choosing acupoints as well as the prescriptions of contemporary times in reference with <千金方(Qianjinfang)>, and to discuss the conception of acupuncture and moxibustion in <千金方(Qianjinfang)>. Method: Based on <千金方(Qianjinfang)>, the parameters were categorized to a certain scope, syndromes in different type were classified. Also numbers of acupoints, route of meridians locations, utilities of special acupoints even prescription methods were statistically analyzed in reference with <千金方(Qianjinfang)>. Results & Conclusion: Acupuncture prescription in <千金方(Qianjinfang)> was mostly presented by single acupoint and this was basic prescription of its rule of choosing acupoints. Choosing acupoint for the majority of various diseases started from choosing meridians with the disorder, but no rules for choosing acupoints was clear. There was basically various methods in choosing acupoints, but little were used by Biao-Ii meridian choosing method (表裏經配穴.) In the high frequency of use, wu-shu acupoints was higher used than other specific acupoints (特定穴). While wu-shu acupoints treated the major parts of the diseases, shu-mo acupoints were strictly used on system. Consequently : The theoretical basis originating the choosing rules of the acupoints and prescriptions was shown in <千金方(Qianjinfang)> as representative clinical reference, through which it was valuable to analyze the prescription rule and specificity of the acupoints.