• 한국균학회지
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• 제38권2호
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• pp.146-151
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• 2010

검체는 2005년 1월1일부터 2009년 12월31일까지 C 병원 진단 검사의학과에 의뢰된 환자의 임상검체 총 366,661예로부터 총 167,955 미생물이 분리되었고 이 중 6,517주의 Candida spp. 분리균을 자동화기기로 동정한 것을 후향적으로 처리 하였다. 배양균주는 smooth하고, 크림색이며, 약간 mucoid하고, 혼탁한 집락을 0.45% salin에 McFarland No 1.6-2.0되게 탁도를 맞춘 후 반응시약이 들어 있는 ID-YST card에 접종하여, VITEK II(bioMerieux Duham NC, USA)의 자동화기기에 넣어 동정하였고, 그 결과는 다음과 같다. Candida spp.의 분리율은 2005년도에 비하여 2009년이 1.9배 증가하였다. 분리빈도가 높은 Candida spp.는 C. albicans 56.4%, C. tropicalis 17.7%, C. glabrata 10.7%, C. parapsilosis 9.5%였고, Candida spp가 10%이상 분리된 검체는 객담 30.1%, 무작위소변 25.0%, 혈액 15.8%, 카테타소변 13.5%였으며, 7%이상 분리된 부서는 호흡기내과 20.5%, 신장내과 11.0%, 감염내과 10.4%, 응급의학과 10.0%, 혈액종양내과 9.6%, 외과 7.5%, 소화기내과 7.4%였다. Candida spp가 증가된 달은 7월이 10.6%였고, 가장 낮게 분리된 달은 2월이 6.1%였다. Candida spp.가 증가된 연령군은 70세 이상 군이 40.1%였고, 가장 낮게 분리된 연령군은 10대가 1.3%였고, 0-49세군(1.3-7.5%)대 50세 이상 군(16.7-40.1%)의 비교에서는 1 : 4.7로 50세 이상 군이 월등히 높았다.

• Journal of Korean Neurosurgical Society
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• 제65권5호
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• pp.665-679
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• 2022

Objective : Patients with mild ischemic stroke experience various sequela and residual symptoms, such as anxious behavior and deficits in movement. Few approaches have been proved to be effective and safe therapeutic approaches for patients with mild ischemic stroke by acute stroke. Sildenafil (SIL), a phosphodiesterase-5 inhibitor (PDE5i), is a known remedy for neurodegenerative disorders and vascular dementia through its angiogenesis and neurogenesis effects. In this study, we investigated the efficacy of PDE5i in the emotional and behavioral abnormalities in rats with mild ischemic stroke. Methods : We divided the rats into four groups as follows (n=20, respectively) : group 1, naïve; group 2, middle cerebral artery occlusion (MCAo30); group 3, MCAo30+SIL-pre; and group 4, MCAo30+SIL-post. In the case of drug administration groups, single dose of PDE5i (sildenafil citrate, 20 mg/kg) was given at 30-minute before and after reperfusion of MCAo in rats. After surgery, we investigated and confirmed the therapeutic effect of sildenafil on histology, immunofluorescence, behavioral assays and neural oscillations. Results : Sildenafil alleviated a neuronal loss and reduced the infarction volume. And results of behavior task and immunofluorescence shown possibility that anti-inflammation process and improve motor deficits sildenafil treatment after mild ischemic stroke. Furthermore, sildenafil treatment attenuated the alteration of theta-frequency rhythm in the CA1 region of the hippocampus, a known neural oscillatory marker for anxiety disorder in rodents, induced by mild ischemic stroke. Conclusion : PDE5i as effective therapeutic agents for anxiety and movement disorders and provide robust preclinical evidence to support the development and use of PDE5i for the treatment of mild ischemic stroke residual disorders.

• Korean Journal of Radiology
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• 제24권7호
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• pp.647-659
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• 2023

Objective: The study was conducted to investigate the effect of correct occlusion of the left atrial appendage (LAA) on intracardiac blood flow and thrombus formation in patients with atrial fibrillation (AF) using four-dimensional (4D) flow magnetic resonance imaging (MRI) and three-dimensional (3D)-printed phantoms. Materials and Methods: Three life-sized 3D-printed left atrium (LA) phantoms, including a pre-occlusion (i.e., before the occlusion procedure) model and correctly and incorrectly occluded post-procedural models, were constructed based on cardiac computed tomography images from an 86-year-old male with long-standing persistent AF. A custom-made closed-loop flow circuit was set up, and pulsatile simulated pulmonary venous flow was delivered by a pump. 4D flow MRI was performed using a 3T scanner, and the images were analyzed using MATLAB-based software (R2020b; Mathworks). Flow metrics associated with blood stasis and thrombogenicity, such as the volume of stasis defined by the velocity threshold ($\left|\vec{V}\right|$ < 3 cm/s), surface-and-time-averaged wall shear stress (WSS), and endothelial cell activation potential (ECAP), were analyzed and compared among the three LA phantom models. Results: Different spatial distributions, orientations, and magnitudes of LA flow were directly visualized within the three LA phantoms using 4D flow MRI. The time-averaged volume and its ratio to the corresponding entire volume of LA flow stasis were consistently reduced in the correctly occluded model (70.82 mL and 39.0%, respectively), followed by the incorrectly occluded (73.17 mL and 39.0%, respectively) and pre-occlusion (79.11 mL and 39.7%, respectively) models. The surfaceand-time-averaged WSS and ECAP were also lowest in the correctly occluded model (0.048 Pa and 4.004 Pa^-1, respectively), followed by the incorrectly occluded (0.059 Pa and 4.792 Pa^-1, respectively) and pre-occlusion (0.072 Pa and 5.861 Pa^-1, respectively) models. Conclusion: These findings suggest that a correctly occluded LAA leads to the greatest reduction in LA flow stasis and thrombogenicity, presenting a tentative procedural goal to maximize clinical benefits in patients with AF.

• 한국임상수의학회지
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• 제40권6호
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• pp.399-407
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• 2023

Chronic kidney disease (CKD) occurs in more than 15% of the dogs over 10 years of age and causes irreversible renal function deterioration. Therefore, it is important to diagnose CKD early and treat the disease properly. The purpose of this study aimed to to evaluate the clinical utility of urine albumin/creatinine ratio (ACR) using POC (point-of-care) device as an early detection urinary biomarker in CKD dogs and to confirm the correlation between ACR and other known CKD biomarkers. Urine and serum samples were obtained from 50 healthy dogs and 50 dogs with CKD. Serum blood urea nitrogen (BUN), creatinine, and symmetric dimethylarginine (SDMA) concentrations, and urine protein creatinine ratio (UPC) were measured. Urine specific gravity (USG) was evaluated using refractometer, and ACR was measured using an i-SENS A1Care analyzer. The ACR values of dogs with CKD were significantly different from those of healthy dogs (p < 0.001), as with other renal biomarkers. ACR showed significant differences between healthy dogs and dogs with CKD at every IRIS stage (p < 0.005), whereas no significant differences were observed between dogs with CKD IRIS stage I and healthy dogs with UPC. There are significant positive correlation between ACR and BUN (r = 0.611, p < 0.001), creatinine (r = 0.788, p < 0.001), SDMA (r = 0.747, p < 0.001), and UPC (r = 0.784, p < 0.001), and significant negative correlation between ACR and USG (r = -0.700, p < 0.001). In receiver operator characteristic curve analysis, the area under the curve (AUC) was 0.982 (95% CI 0.963-1.000, p < 0.001), with an optimal cut-off value of 64.20 mg/g (94% sensitivity and 94% specificity). Thus, ACR is a useful urinary biomarker for the early diagnosis of proteinuria in CKD and combined use of ACR and other renal biomarkers may be helpful for early diagnosis and prevention of CKD in dogs.

• Journal of Chest Surgery
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• 제31권11호
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• pp.1023-1030
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• 1998

연구배경 : 인체내 이식된 생체조직들은 석회화 변성으로 인해 그 내구성이 단축된다. 이러한 조직들을 이식하기전에 글루타르알데하이드(GA)로 고정후 sulphonated polyethyleneoxide(PEO-SO3)를 결합시키고 또한 조직내 의 잔유 알데히드기를 제거함으로써 석회화에 대한 내구성을 향상시킬 수 있다 재료 및 방법 : PEO-SO3 처리법과 잔유 알데히드기 제거의 석회화 방지 효과를 알아보기 위하여 GA 고정 후 PEO-SO3로 처리한 소 심낭 첨포를 잔유 알데히드기 제거를 위해 무균 생리식염수에 보존한 saline 군과 0.65% GA 용액 에 보존한 GA군, 그리고 이미 상품화되어 임상에서 사용되고 있는 product군을 8마리 개의 경동맥과 대퇴동 맥 및 복막에 이식하여 6주 후 적출하여 칼슘량 및 병리조직 소견에 대해 알아보았다. 결과 : 경동맥에서 채취한 조직첨포의 칼슘량은 saline군에서 GA군과 product군에 비해 유의하게 적었다 (saline; 2.89$\pm$0.31 vs. GA; 6.14$\pm$1.08 vs. product; 22.82$\pm$5.00 mg/g; p< .05). 대퇴동맥과 복막 이식 첨포에서의 칼슘량 또한 saline군에서 다른 두 군에 비해 가장 적었으나 통계적인 유의성은 없었다. 한편 병리조직 검사에서는 GA군에서 다른 두 군에 비해 조직의 석회화로 인한 변성 파괴가 심하였다. 결론 : 본 연구에서 소 심낭 조직 첨포와 결합한 PEO-SO3는 석회화를 감소시키고 또한 조직내 잔유 알데히드기 를 생리식염수로 세척하여 제거함으로써 항석회화 효과를 증가시킬 수 있었다. 결론적으로 PEO-SO3로 처리한 소 심낭 조직은 석회화 변성에 대한 강한 저항성을 보임으로써 심혈관 첨포나 인공 조직 판막 개발 에 도움를 줄 수 있을 것이다.