• 제목/요약/키워드: Bio-film

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Effect of Type I Collagen on Hydroxyapatite and Tricalcium Phosphate Mixtures in Rat Calvarial Bony Defects

  • Kim, Jung-Hwan;Kim, Soung-Min;Kim, Ji-Hyuck;Kwon, Kwang-Jun;Park, Young-Wook
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.34 no.1
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    • pp.36-48
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    • 2008
  • To repair bone defects in the oral and maxillofacial field, bone grafts including autografts, allografts, and artificial bone are used in clinical dentistry despite several disadvantages. The purpose of this study was to evaluate new bone formation and healing in rat calvarial bone defects using hydroxyapatite (HA, $Ca_{10}[PO_4]_6[OH]_2,\;Bongros^{(R)}$, Bio@ Co., KOREA) and tricalcium phosphate (${\beta}-TCP,\;Ca_3[PO_4]_2$, Sigma-Aldrich Co., USA) mixed at various ratios. Additionally, this study evaluated the effects of type I collagen (Rat tail, BD Biosciences Co., Sweden) as a basement membrane organic matrix. A total of twenty, 8-week-old, male Sprague-Dawley rats, weighing 250-300g, were divided equally into a control group (n=2) and nine experimental groups (n=2, each). Bilateral, standardized transosseous circular calvarial defects, 5.0 mm in diameter, were created. In each experimental group, the defect was filled with HA and TCP at a ratio of 100:0, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 0:100 with or without type I collagen. Rats were sacrificed 4 and 8 weeks post-operation for radiographic (standardized plain film, Kodak Co., USA), histomorphologic (H&E [Hematoxylin and Eosin], MT [Masson Trichrome]), immunohistochemical staining (for BMP-2, -4, VEGF, and vWF), and elementary analysis (Atomic absorption spectrophotometer, Perkin Elmer AAnalyst $100^{(R)}$). As the HA proportion increased, denser radiopacity was seen in most groups at 4 and 8 weeks. In general radiopacity in type I collagen groups was greater than the non-collagen groups, especially in the 100% HA group at 8 weeks. No new bone formation was seen in calvarial defects in any group at 4 weeks. Bridging bone formation from the defect margin was marked at 8 weeks in most type I collagen groups. Although immunohistochemical findings with BMP-2, -4, and VEGF were not significantly different, marked vWF immunoreactivity was present. vWF staining was especially strong in endothelial cells in newly formed bone margins in the 100:0, 80:20, and 70:30 ratio type I collagen groups at 8 weeks. The calcium compositions from the elementary analysis were not statistically significant. Many types of artificial bone have been used as bone graft materials, but most of them can only be applied as an inorganic material. This study confirmed improved bony regeneration by adding organic type I collagen to inorganic HA and TCP mixtures. Therefore, these new artificial bone graft materials, which are under strict storage and distribution systems, will be suggested to be available to clinical dentistry demands.

Effect of Particle Size and Mixing Ratio on Quality of Fluidized Coated Vitamin C (입자크기와 혼합비에 따른 유동층 코팅 비타민 C의 품질 특성)

  • Park, Su-Jung;Hwang, Sung-Hee;Chung, Hun-Sik;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.14 no.4
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    • pp.364-368
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    • 2007
  • The purpose of this study was to improve the stability and the processing property of vitamin C. Vitamin C was coated according to particle size(80-100 mesh, 100-140 mesh) and mixing ratio(1:1.6, 1:2.5, 1:3(w/w)) with coating solution(8% Zein-DP, 6% HPMC-FCC), and then the quality characteristics of fluidized bed micro coated vitamin C were investigated. The coating efficiency and the thickness of coating film were higher in $80{\sim}100$ mesh particle than in $100{\sim}140$ mesh particles, and coating efficiency was decreased as the coating material was increased. The distribution range of particle was more narrow in mixing ratio of 1:3(w/w) than in the other. DPPH radical scavenging activity was not affected by the particle size and the mixing ratio. There was no difference between the coating materials in terms of the quality characteristics. The optimum coating condition for fluidized bed micro-coating of vitamin C powder was selected as the particle size of $80{\sim}100$ mesh and the mixing ratio with coating solution of 1:3(w/w).

Characteristics of the Leaf Fiber Plants Cultivated in Korea (국내 재배 엽맥섬유의 특성에 관한 연구)

  • Lee, Hye-Ja;Kim, Nam-Eun;Yoo, Hye-Ja;Han, Young-Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.33 no.5
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    • pp.711-720
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    • 2009
  • Leaf fibers have many good properties; they are strong, long, cheap, abundant and bio-degradable. Since they, however, contain a great quantity of non-cellulose components, they have been used for the materials of mats, ropes, bags and nets rather than those of clothing. In this study, we investigated the characteristics of leaf fibers in order to promote the use of leaf fibers for the materials of clothing as well as develop the high value-added textile fibers. Leaf fiber plants including New Zealand Flax, Henequen and Banana plant, which have various nature and shape, were used. New Zealand Flax and Henequen leaves were cut from lower part of plants. Banana leaves and pseudo-stems were peeled and cut from the stem of Banana plants. First, the thin outer skins like film of leaves, veins and stems were removed before retting. The chemical retting had been processed for 1hour, at 100 in 0.4% $H_2SO_4$ aqueous solution(liquid ratio 50:1). Then, the retted leaf fibers had been soaked for 1hour, at room temperature in 0.5% NaClO solution(v/v) to remove the miscellaneous materials. We investigated the physical characteristics of three leaf fibers including the transversal and longitudinal morphology, the contents(%) of pectin, lignin and hemicellulose, the length and diameter of fibers, the tensile strength of the fiber bundles, and the fiber crystallinity and the moisture regain(%). The lengths of fiber from three leaf fibers were similar to their leaf lengths. The fiber bundles were composed of the cellulose paralleled to the fiber axis and the non-cellulose intersecting at right angle with the fiber axis. The diameters of New Zealand Flax, Henequen and Banana fibers were $25.13{\mu}m$, $18.16{\mu}m$ and $14.01{\mu}m$, respectively and their tensile strengths were 19.40 Mpa, 32.16 Mpa and 8.45 Mpa, respective. The non-cellulose contents of three leaf fibers were relatively as high as 40%. If the non-cellulose contents of leaf fibers might be controlled, leaf fibers could be used for the materials of textile fiber, non-wovens and Korean traditional paper, Hanjee.

Removal Characteristics of Geosmin in a Slow Sand Filteration Process (완속 모래여과 공정에서의 Geosmin 제거 특성)

  • Son, Hee-Jong;Yoom, Hoon-Sik;Jang, Seong-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.8
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    • pp.754-760
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    • 2010
  • Geosmin removal by biodegradation was investigated in lab-scale slow sand filtration column with different empty bed contact times (EBCTs) and water temperature. Schmutzdecke layer was built up after 30 days operation and biomass and activity were $4.5{\times}10^6\;CFU/g$ and $3.42\;mg{\cdot}C/m^3{\cdot}hr$, respectively. The attached bio-film microorganisms in schmutzdecke layer were isolated and identified. The dominant species was Pseudomonas sp. that had occupied 56%. Removal efficiencies of dissolved organic carbon (DOC) and geosmin were 27% and 95% after 30 days operation. In lab-scale slow sand filtration column, geosmin and DOC removal efficiencies were 62% and 10% at $5^{\circ}C$, respectively. And increasing water temperature ($15^{\circ}C$ and $25^{\circ}C$) increased the geosmin and DOC removal efficiencies (88~100% and 25~42%) in lab-scale slow sand filtration column. Geosmin and DOC biodegradation rates (k) in the schmutzdecke layer (in the upper 5 cm filter bed) were $1.842{\sim}15.965\;hr^{-1}$1 and $0.253{\sim}1.123\;hr^{-1}$, respectively. It were about 18~32 times and 20~51 times of the rates in the deeper filter bed (5~60 cm).

Process Optimization for the Industrialization of Transparent Conducting Film (투명 전도막의 산업화를 위한 공정 최적화)

  • Nam, Hyeon-bin;Choi, Yo-seok;Kim, In-su;Kim, Gyung-jun;Park, Seong-su;Lee, Ja Hyun
    • Industry Promotion Research
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    • v.9 no.1
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    • pp.21-29
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    • 2024
  • In the rapidly advancing information society, electronic devices, including smartphones and tablets, are increasingly digitized and equipped with high-performance features such as flexible displays. This study focused on optimizing the manufacturing process for Transparent Conductive Films (TCF) by using the cost-effective conductive polymer PEDOT and transparent substrate PET as alternatives to expensive materials in flexible display technology. The variables considered are production speed (m/min), coating maximum temperature (℃), and PEDOT supply speed (rpm), with surface resistivity (Ω/□) as the response parameter, using Response Surface Methodology (RSM). Optimization results indicate the ideal conditions for production: a speed of 22.16 m/min, coating temperature of 125.28℃, and PEDOT supply at 522.79 rpm. Statistical analysis validates the reliability of the results (F value: 18.37, P-value: < 0.0001, R2: 0.9430). Under optimal conditions, the predicted surface resistivity is 145.75 Ω/□, closely aligned with the experimental value of 142.97 Ω/□. Applying these findings to mass production processes is expected to enhance production yields and decrease defect rates compared to current practices. This research provides valuable insights for the advancement of flexible display manufacturing.

Growth and Rooting Rate of 'Maehyang' Strawberry as Affected by Irrigation Method on Cutting Propagation in Summer Season (하절기 '매향' 딸기의 삽목 번식 시 관수방법에 따른 생육 및 발근율)

  • Kim, Hyeon Min;Kim, Hye Min;Jeong, Hyeon Woo;Lee, Hye Ri;Jeong, Byoung Ryong;Kang, Nam Jun;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.27 no.2
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    • pp.103-110
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    • 2018
  • This study was conducted to investigate the optimum irrigation method for propagation of cutting strawberry ($Fragaria{\times}ananassa$ Duch. cv. Maehyang) plantlet in summer season. The cutting strawberry plantlets were planted in 24-cell tray ($60{\times}34{\times}10cm$) filled with commercial mixed medium (Tosilee) and placed in semi closed-type small plastic tunnel. Each semi closed-type small plastic tunnel was treated as follows; control (non-treatment), over head irrigation (twice a day), capillary mats irrigation (twice a day), or fog irrigation (30 minutes turn on and 10 minutes off from 8:00 to 18:00). The strawberry plantlets were rooted during 8 days in the semi closed-type small plastic tunnel, and then plastic film was removed. Growth parameters, such as plant height, root length, number of primary roots, petiole length, leaf length, leaf width, crown diameter, SPAD, leaf area, fresh and dry weights of the shoot and root, were measured at 61 days after cutting. Relative humidity in tunnel was high in the order by fog irrigation, over head irrigation, capillary mats, and the control as 72.5, 56.3, 45.8, and 29% on average, respectively. However, the air temperature was similar in all treatments. On the 4 and 8 days, the rooting rate of strawberry plantlet was significantly higher in the over head irrigation and fog irrigation treatments. Plant height, petiole length, crown diameter, and leaf area were highest in the over head irrigation and fog irrigation treatments. In addition, fresh and dry weights of shoot were greater in over head irrigation and fog irrigation treatments than the others. Dry weight of root was differed significantly heaviest in the fog irrigation treatment. However, root length, no. of primary roots, SPAD value, and fresh weight of root were not significantly different in all treatments. These results indicated that growth and rooting for propagation of cutting strawberry plantlet 'Maehyang' were best achievement in the over head irrigation and fog irrigation treatments.

Responses to 1-MCP during Storage of Kimchi Cabbage Ryouckgwang Cultivar (배추 력광 품종의 저장 중 1-MCP에 대한 반응)

  • Hong, Sae Jin;Kim, Byung-Sup;Kim, Byeong-Sam;Eum, Hyang Lan
    • Journal of Bio-Environment Control
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    • v.27 no.2
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    • pp.125-131
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    • 2018
  • The effect of 1-methylcyclopropene (1-MCP) in the storability of kimchi cabbage at cold storage condition was investigated. Kimchi cabbage (Brassica campestris L. cv Ryouckgwang) was divided four groups, forced air cooling (FAC), FAC + 0.03 mm linear low density polyethylene liner (Liner), $FAC+2{\mu}L{\cdot}L^{-1}$ 1-MCP (1-MCP), and FAC + 1-MCP + Liner. After each treatment kimchi cabbage was stored at $2^{\circ}C$, 95% RH. Quality parameters were weight loss, soluble solids content (SSC), firmness, and color ($CIE\;L^*$, $a^*$, $b^*$, chroma, hue angle). Weight loss during storage was showed significant difference by Liner treatment. In particular FAC + 1-MCP + Liner treatment showed 12.5% reduction after 6 weeks of storage period and minimized the weight loss rate compared to other treatments. SSC of kimchi cabbage was $2.5^{\circ}Brix$ at harvest and FAC + 1-MCP + Liner treatment maintained the SSC until 3 weeks, while in other treatments gradually were increased. The firmness of kimchi cabbage was 24.0 N immediately after harvest and the firmness at harvest time tended to be maintained at 22.6 N after 6 weeks of storage in FAC + 1-MCP + Liner treatment. During the storage period, the color change of the kimchi cabbage leaf can be confirmed by $CIE\;a^*$ and hue angle value. 1-MCP treatment alone did not affect the color change, however 1-MCP + Liner treatment was able to maintain the chromaticity at harvest time while minimizing the change of $CIE\;a^*$ and hue angle. These results suggest that 1-MCP treatment is not effective for the storage of kimchi cabbage but can be maintained for up to 6 weeks when treated with Liner.

Growth Characteristics of Common Ice Plant (Mesembryanthemum crystallinum L.) on Nutrient Solution, Light Intensity and Planting Distance in Closed-type Plant Production System (완전제어형 식물 생산 시스템에서 배양액, 광도 및 재식거리에 따른 Common Ice Plant의 생육 특성)

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • Journal of Bio-Environment Control
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    • v.25 no.2
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    • pp.89-94
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    • 2016
  • This study was conducted to determine the optimum nutrient solution, pH, irrigation interval, light intensity and planting density to growth of common ice plant (Mesembryanthemum crystallinum L.) in a closed-type plant production system. Three-band radiation type fluorescent lamps with a 12-h photoperiod were used. Nutrient film technique systems with three layers were used for the plant growth system. Environmental conditions, such as air temperature, relative humidity and $CO_2$ concentration were controlled by an ON/OFF operation. Treatments were comparison of the nutrient solution of Horticultural Experiment Station in Japan (NHES) and the nutrient solution of Jeju National University (NJNU), pH 6.0 and 7.0, irrigation interval 5 min and 10 min, light intensity 90 and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and within-row spacing 10 cm, 15 cm, 20 cm and 25 cm with between-row spacing 15 cm. Optimum macronutrients were composed N 7.65, P 0.65, K 4.0, Ca 1.6 and Mg $1.0mM{\cdot}L^{-1}$. There were no significant interactions between pH 6.0 and 7.0 about shoot fresh weight and shoot dry weight of common ice plant. Irrigation interval 5 min and 10 min was also the same result. Shoot fresh weight and shoot dry weight were highest at $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Shoot fresh weight and shoot dry weight were decreased according to increasing the planting density. From the above results, we concluded that optimum nutrient solution, optimum levels of pH, irrigation interval, light intensity and planting density were 6.0-7.0 and 10 min, $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and $15{\times}15cm$, respectively for growth of common ice plant in a closed-type plant production system.

Characteristics of Temperature, Humidity and PPF Distribution by Covering Method and Environmental Control in Double Covering Greenhouse (이중피복 온실의 피복방법과 환경조절에 따른 온습도 및 광합성유효광량자속 분포 특성)

  • Lee, Hyun-Woo;Sim, Sang-Youn;Kim, Young-Shik
    • Journal of Bio-Environment Control
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    • v.21 no.1
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    • pp.1-11
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    • 2012
  • The objective of the present study is to provide data needed to find double covering method to be able to improve environment of temperature, humidity and PPF in tomato greenhouse. The distribution charts of temperature, humidity and PPF which were measured in environment control conditions such as thermal insulation, air heating, roof ventilation and air fog cooling in conventional and air inflated double layers greenhouses were drawn and analysed. The thermal insulation effect of the air inflated greenhouse was the same as that of conventional greenhouse because the temperature between insulation curtain and roof covering material was equal in heating season. The ventilation effect of the air inflated greenhouse was superior to the conventional greenhouse. The temperature distribution in the fog cooled greenhouse was uniform and the cooling effect was about $3.5^{\circ}C$. The condensation on the roof covering surface could be controlled by removing the moisture between insulation curtain and roof covering by using humidifier. The PPF of conventional greenhouse was more decreased than the air inflated greenhouse as time went by because the transmittance of conventional greenhouse declined by dust collected on the inside plastic film owing to rolling up and down operation for ventilation.

Effects of $\textrm{NO}_3$-N:$\textrm{NH}_4$-N Ratio and Elevated $\textrm{CO}_2$ on Growth and Quality of Lactuca sativa L. in Nutrient Film Technique (NFT재배에서 $\textrm{CO}_2$ 시용과 배양액의 $\textrm{NO}_3$:$\textrm{NH}_4$비율이 결구상추의 생육 및 품질에 미치는 영향)

  • 원선이;조영렬;이용범
    • Journal of Bio-Environment Control
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    • v.5 no.2
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    • pp.120-130
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    • 1996
  • Crisphead lettuce(Lactuca sativa L.) was grown in NFT to investigate the effects of NO$_3$-N and NH$_4$-N ratio in nutrient solution and elevated $CO_2$ treatment in the crisphead lettuce growth. This experiment has been conducted under three different ratios of NO$_3$-N:NH$_4$-N(100:0, 75:25, 50:50) with two $CO_2$ concentration (control, 1500ppm ). The results are as follows; 1. In the case of not controlling pH and EC in nutrient solution, pH was gradually increased in NO$_3$-N:NH$_4$-N=100:0 treatment but rapidly decreased in the nutrient solution 2. Daily changes of NO$_3$-N and NH$_4$-N were observed without controlling the nutrient solution. In the treatments of NO$_3$-N:NH$_4$-N ratios were 75:25 and 50:50, NO$_3$-N absorption rates were 27.7% and 26.1%, while NH$_4$-N absorption rates were 87.9% and 71.2%, respectively. 3. There was little differences in total nitrogen of leaves. However phosphorus, potassium, calcium and magnesium contents were highly shown in the treatment of $CO_2$ 1500ppm and 100:0 ratio of NO$_3$-N:NH$_4$-N. 4. Higher $CO_2$ assimilation rate was shown in plants grown under $CO_2$ 1500ppm and 100:0 ratio of NO$_3$-N:NH$_4$-N. It dropped significantly with the increase of NH$_4$- N rates in nutrient solution. 5. Fresh weight, leaf number, root length and root weight of crisphead lettuce were far better in the treatment of $CO_2$ 1500ppm and 100:0 ratio of NO$_3$-N:NH$_4$-N. Growth differences by $CO_2$ elevation were not shown in other NO$_3$-N:NH$_4$-N treatments. 6. The highest nitrate contents of leaves were shown in NO$_3$-N single treatment but shown the lowest vitamin C contents. Nitrate contents of leaves were decreased by $CO_2$ but the effect was slight treatment.

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