• Transactions of the Korean hydrogen and new energy society
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• v.22 no.2
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• pp.257-264
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• 2011

This study discribes performance of DEFC (Direct Ethanol Fuel Cell) utilized bio-ethanol based on fruit wastes. To produce the bio-ethanol, fruit wastes were treated at temperature $120^{\circ}C$ and 90minutes in acid pre-treatment. After pre-treatment was done, alcohol fermentation process was running. Initial alcohol concentration was 5%. Using the multi coloumn distillation system, more than 95% ethanol was distilled and each component of bio-ethanol was analyzed. In DEFC performance test, it was revealed that cell performance was much higher than that of ethanol. Comparing ethanol with mixed fuel (bio-ethanol (10%) + ethanol (90%)), the performance of ethanol was higher than that of mixed fuel. Even though the bio-ethanol from the fruit wastes is corresponded with transport ethanol standards, it thought that organic matter in bio-ethanol could be negative effect on fuel cell.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.45 no.2
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• pp.114-121
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• 2009

There are increased in using the bio-ethanol, as the carbon neutral attracts many researchers due to a reduction in carbon dioxide spotted as the global warming gas. A gasoline engine with 100% of the bioethanol was developed and used in Brazil already, but researches of using the bio-ethanol in diesel engines are lack. In this study, combustion tests with blend fuel of the gas oil and bio ethanol by 50% maximally due to a low cetane number of bio-ethanol were accomplished as a basic study of introduction of using the bioethanol in diesel engines. The result was that smoke emission was decreased with increase in proportion of the bio-ethanol, due to the increase of a amount of pre-mixed combustion with ignition delay. Although the amount of $CO_2$ is reduced according as the bio-ethanol is used(carbon neutral), the emission of $CO_2$ with increase in the proportion of the bio-ethanol was more increased due to lower a heat value of bio-ethanol than gas oil.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.1
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• pp.37-43
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• 2010

The purpose of this paper is to investigate the effect of air-fuel ratio on the combustion and emissions characteristics of spark ignition (SI) gasoline engine fueled with bio-ethanol. A 1.6L SI engine with 4 cylinders was tested on EC dynamometer. In addition, lambda sensor and lambda meter were connected with universal ECU to control the lambda value which is varied from 0.7 to 1.3. The engine performance and combustion characteristics of bio-ethanol fuel were compared to those obtained by pure gasoline. Furthermore, the exhaust emissions such as carbon monoxide (CO), unburned hydrocarbon (HC), oxides of nitrogen ($NO_X$) and carbon dioxide ($CO_2$) were measured by emission analyzers. The results showed that the brake torque and cylinder pressure of bio-ethanol fuel were slightly higher than those of gasoline fuel. Brake specific fuel consumption (BSFC) of bio-ethanol was increased while brake specific energy consumption (BSEC) was decreased. The exhaust emissions of bio-ethanol fuel were lower than those of gasoline fuel under overall experimental conditions. However, the specific emission characteristics of the engine with bio-ethanol fuel were influenced by air-fuel ratio.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.8
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• pp.582-588
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• 2008

An experimental investigation was conducted to investigate the effect of Bio-ethanol fuel on the engine performance and exhaust emission characteristics under various engine operating conditions. To investigate the effect of bio-ethanol fuel, the commercial 1.6L SI engine equipped with 4 cylinder was tested on EC dynamometer. The engine performance including brake torque, brake specific fuel consumption, and barke specific energy consumption of bio-ethanol fuel was compared to those obtained by pure gasoline. Furthermore, the exhaust emissions were analyzed in terms of regulated exhaust emissions such as unburned hydrocarbon, oxides of nitrogen, and carbon monoxide.Result of this work shows that the effect of blending of ethanol to gasoline caused drastic decrease of emissions under various operating conditions. Also, improved engine performance such as brake torque and brake power were indicated for bio-ethanol fuel.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.4
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• pp.131-137
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• 2012

This article is about using the fuel mixed with 10% and 20% bio-ethanol to gasoline for the engine as a way to reduce carbon emission before commercializing future automobiles like fuel cell cars. The fuel mixed with 10% and 20% bio-ethanol showed output equivalent to that of the previous gasoline fuel. CO and $CO_2$ emission was somewhat reduced, but the difference was not significant. And the consumption of the fuel increased slightly. However, bio-ethanol is produced from bio mass growing with the absorption of carbon dioxide, so the total amount of carbon dioxide did not increase according to the result. In NOx, as the use of ethanol increases, the effect of reduction gets greater, and the emission of oxygen showed almost no change compared with gasoline.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.7
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• pp.609-615
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• 2015

In this study, the combustion and exhaust emission characteristics in a gasoline direct injection engine with variations of the bio-ethanol-gasoline blending ratio and the excess air factor were investigated. To investigate the effects of the excess air factor and the bio-ethanol blends with gasoline, combustion characteristics such as the in-cylinder combustion pressure, rate of heat release (ROHR), and the fuel consumption rate were analyzed. The reduction of exhaust emissions such as carbon monoxide (CO), unburned hydrocarbon (HC), and nitrogen oxides ($NO_x$) were compared with those of gasoline fuel with various excess air factors. The results showed that the peak combustion pressure and ROHR of bio-ethanol blends were slightly higher and were increased as bio-ethanol blending ratio is increased. Brake specific fuel consumption increased for a higher bio-ethanol blending ratio. The exhaust emissions decreased as the bio-ethanol blending ratio increased under all experimental conditions. The exhaust emissions of bio-ethanol fuels were lower than those of gasoline.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1630-1636
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• 2010

During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1 was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore, the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.

• Applied Chemistry for Engineering
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• v.20 no.5
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• pp.517-521
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• 2009

In this study, the productivity of bio-ethanol obtained from various brown-algae raw materials was examined. Brown-algae polysaccharide, consisting of alginate and laminaran, is usable for the effective production of bio-ethanol if it is hydrolyzed to monomer unit. The objective of this study is the production of bio-ethanol from brown-algae using a heat-treatment and acid-treatment. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 and Pachysolen tannophilus KCTC 7937 strains. Laminaran japonica was higher than Sagassum fulvellum and Hizikia fusiformis, Laminaran japonica optimum pre-treatment is used to derive the ethanol production of Saccharomyces cerevisiae KCCM1129 and Pachysolen tannophilus KCTC 7937 respectively 9.16 g/L, 9.80 g/L. The maximum output of Sargassum fulvellum and Hizikia fusiformis was very low as 0.22 g/L.

• Applied Chemistry for Engineering
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• v.21 no.2
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• pp.154-161
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• 2010

In the study, the effect of acid and salt concentrations during the production of bio-ethanol from various brwon-algae raw materials was investigated. Especially, the possibility of the conversion of various polysaccarides contained in Laminaria japonica was studied. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 strains in Laminaria japonica. The maximum bio-ethanol production of 2.09 g/L using heat-treatment of Laminaria japonica was achieved. The optimum concentration for reducing sugar conversion by Laminaria japonica was found to be 3.95 g/L at the HCl concentration of 0.1 N. But bio-ethanol production was higher than the case without the non-acid pretreatment. Among the various polysaccharides, only mannitol produced maximum 3.09 g/L bio-ethanol. In case of laminaran, the ethanol was produced only at 0.15 g/L only in 0.1 N HCl pretreatment medium and cell growth was higher than other pretreatment.

• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.433-437
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• 2016

Consumption of high doses of ethanol can lead to amnesia, which often manifests as a blackout. These blackouts experienced by ethanol consumers may be a major cause of the social problems associated with excess ethanol consumption. However, there is currently no established treatment for preventing these ethanol-induced blackouts. In this study, we tested the ethanol extract of the roots of Salvia miltiorrhiza (SM) for its ability to mitigate ethanol-induced behavioral and synaptic deficits. To test behavioral deficits, an object recognition test was conducted in mouse. In this test, ethanol (1 g/kg, i.p.) impaired object recognition memory, but SM (200 mg/kg) prevented this impairment. To evaluate synaptic deficits, NMDA receptor-mediated excitatory postsynaptic potential (EPSP) and long-term potentiation (LTP) in the mouse hippocampal slices were tested, as they are known to be vulnerable to ethanol and are associated with ethanol-induced amnesia. SM (10 and $100{\mu}g/ml$) significantly ameliorated ethanol-induced long-term potentiation and NMDA receptor-mediated EPSP deficits in the hippocampal slices. Therefore, these results suggest that SM prevents ethanol-induced amnesia by protecting the hippocampus from NMDA receptor-mediated synaptic transmission and synaptic plasticity deficits induced by ethanol.