• Mass Spectrometry Letters
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• v.13 no.4
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• pp.166-176
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• 2022

Banana peels are also widely used as bio-adsorbent in the removal of chemicals contaminants and heavy metals from water and soil. GC-MS plays an essential role in the phytochemical analysis and chemo taxonomic studies of medicinal plants containing biologically active components. Intrinsically, with the use of the flame ionization detector and the electron capture detector which have very high sensitivities, Gas chromatography can quantitatively determine materials present at very low concentrations and most important application is in pollution studies. In the present study banana peels were used as bio-adsorbent to remediate the heavy metal contaminated water taken from three different stations located around the industrial belts of Ranipet, Tamilnadu, India. The AAS analysis of the samples shows a decrement of chromium concentration of 98.93%, 96.16% and 96.5% in Station 1, 2 and 3 respectively which proves the efficiency of the powdered peels of Musa paradisiaca. The GC-MS analysis of the control and treated peels of Musa paradisiaca reveals the presence of phytochemicals like Acetic Acid, 1-Methylethyl Ester, DL-Glyceraldehyde Dimer, N-Hexadecanoic Acid, 3-Decyn-2-Ol, 26-Hydroxy, Cholesterol, Ergost-25-Ene-3,5,6,12-Tetrol, (3.Beta.,5.Alpha.,6.Beta.,12.Beta.)-, 1-Methylene-2b-Hydroxymethyl-3, and 3-Dimethyl-4b-(3-Methylbut-2-Enyl)-Cyclohexane in the control banana peels. The banana peels which were used for the treatment reveals the changes and alteration of the phytochemicals. It is concluded that the alteration in phytochemicals of the experimental banana peels were due to adsorption of chromium heavy metal from the sample.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.891-897
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• 2021

There is a need for a technology that can quickly and accurately analyze soil carbon contents. Existing soil carbon analysis methods are cumbersome in terms of professional manpower requirements, time, and cost. It is against this background that the present study leverages the soil physical properties of color and water content levels to develop a model capable of predicting the carbon content of soil sample. To predict the total carbon content of soil, the RGB values, water content of the soil, and lux levels were analyzed and used as statistical data. However, when R, G, and B with high correlations were all included in a multiple regression analysis as independent variables, a high level of multicollinearity was noted and G was thus excluded from the model. The estimates showed that the estimation coefficients for all independent variables were statistically significant at a significance level of 1%. The elastic values of R and B for the soil carbon content, which are of major interest in this study, were -2.90 and 1.47, respectively, showing that a 1% increase in the R value was correlated with a 2.90% decrease in the carbon content, whereas a 1% increase in the B value tallied with a 1.47% increase in the carbon content. Coefficient of determination (R²), root mean square error (RMSE), and mean absolute percentage error (MAPE) methods were used for regression verification, and calibration samples showed higher accuracy than the validation samples in terms of R² and MAPE.

• Hip & pelvis
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• v.35 no.2
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• pp.63-72
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• 2023

The aim of this study was to conduct a meta-analysis of randomized controlled trials (RCTs) for comparison of the effectiveness of pericapsular nerve group (PENG) block with that of other analgesic techniques for reduction of postoperative pain and consumption of opioids after total hip arthroplasty (THA). A search of records in the PubMed, Embase, and Cochrane Library, and ClinicalTrials.gov databases was conducted in order to identify studies comparing the effect of the PENG block with that of other analgesics on reduction of postoperative pain and consumption of opioids after THA. Determination of eligibility was based on the PICOS (participants, intervention, comparator, outcomes, and study design) criteria as follows: (1) Participants: patients who underwent THA. (2) Intervention: patients who received a PENG block for management of postoperative pain. (3) Comparator: patients who received other analgesics. (4) Outcomes: numerical rating scale (NRS) score and opioid consumption during different periods. (5) Study design: clinical RCTs. Five RCTs were finally included in the current meta-analysis. Significantly lower postoperative opioid consumption at 24 hours after THA was observed in the group of patients who received the PENG block compared with the control group (standard mean difference=-0.36, 95% confidence interval -0.64 to -0.08). However, no significant reduction in NRS score at 12, 24, and 48 hours after surgery and opioid consumption at 48 hours after THA was observed. The PENG block showed better results for opioid consumption at 24 hours after THA compared with other analgesics.

• Journal of the Korean Society of Industry Convergence
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• v.21 no.4
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• pp.159-165
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• 2018

In electric agricultural machine a reduction gear is needed to convert the high speed rotation motion generated by DC motor to lower speed rotation motion used by the vehicle. The reduction gear consists of several spur gears. Spur gears are the most easily visualized gears that transmit motion between two parallel shafts and easy to produce. The modelling and simulation of spur gears in DC motor reduction gear is important to predict the actual motion behaviour. A pair of spur gear tooth in action is generally subjected to two types of cyclic stress: contact stress and bending stress. The stress may not attain their maximum values at the same point of contact fatigue. These types of failure can be minimized by analysis of the problem during the design stage and creating proper tooth surface profile with proper manufacturing methods. To improve its life expectation in this study modal and stress analysis of reduction gear is simulated using ANSYS workbench based on finite element method (FEM). The modal analysis was done to understand reduction gear deformation behaviour when vibration occurs. FEM static stress analysis is also simulated on reduction gear to simulate the gear teeth bending stress and contact stress behaviour.

• The Journal of the Korea Contents Association
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• v.16 no.12
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• pp.70-77
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• 2016

In this study, we aim to obtain the fundamental data needed for the objective analysis and bio-mimetics of leeches, using x-ray imaging with radiation that allows 3D analysis of the detailed anatomic structures of leeches. It was confirmed that through using radiation, detailed images of leeches can be obtained, allowing 3D analysis of leeches' anatomical structures. Also, since the data obtained through the radiation allows you to observe the micro-structure, it can serve as a good resource for component analysis, as well as physiological and functional research. More, it is hoped to contribute to further research in the areas of bio-mimetics using leeches.

• BMB Reports
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• v.37 no.4
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• pp.474-479
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• 2004

The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ~21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.

• Journal of Korean Society of Rural Planning
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• v.25 no.3
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• pp.89-102
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• 2019

This study was conducted to investigate priority recognition of experts and related facility operators in establishing policies for revitalizing agro-healing services. After reviewing related studies, 5 policy division and 19 detailed sector were drawn, including improving accessibility, improving promotion and information provision method, improved public relations and information provision, diversification of facility and program, financial support, and expansion of expert and specialized institutions. A questionnaire survey was conducted on agro-healing experts, agro-healing facility operators, social welfare experts, and social welfare facility operators. The AHP analysis indicated that agro-healing experts and facility operators had the highest priority in the division of expansion of expert and specialized institutions, while social welfare experts and facility operators had the highest priority in financial support. The IPA analysis was conducted to identify the priority of each policy sector. Respondents recognized above normal importance to policy divisions, but were relatively aware of the importance of improving accessibility, with overall satisfaction level appearing to be moderate. The results of this study are expected to provide useful information for establishing agro-healing policies and for establishing effective strategies.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.254-254
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• 2022

Astragalus membranaceus (A. membranaceus) has traditionally been used as a medicinal plant in East Asia for the treatment ofvarious diseases. A. membranaceus belongs to the legume family and is known to be rich in substances such as flavonoids and saponins. Recent pharmacological studies of A. membranaceus have shown that the plant has immunomodulatory, anti-oxidant, anti-cancer, and anti-inflammatory effects. However, knowledge of major biosynthetic pathways in A. membranaceu is still lacking. Recently developed sequencing techniques enable high-quality transcriptome analysis in plants, which is recognized as an important part in elucidating the regulatory mechanisms of many plant secondary metabolic pathways. However, it is difficult to predict the number of transcripts because plant transcripts contain a large number of isoforms due to alternative splicing events, which can vary depending on the assembly platform used. In this study, we constructed three unigene sets using Pac-Bio isoform sequencing, Trinity and rnaSPAdes assembly for detailed transcriptome analysis mA. membranaceus. Furthermore, all genes involved in the flavonoid biosynthetic pathway were searched from three unigene sets, and structural comparisons and expression profiles between these genes were analyzed. The isoflavone synthesis was active in most tissues. Flavonol synthesis was mainly active in leaves and flowers, and anthocyanin synthesis was specific in flowers. Gene structural analysis revealed structural differences in the flavonoid-related genes derived from the three unigene sets. This study suggests the need for the application of multiple unigene sets for the analysis of key biosynthetic pathways in plants.

• The Plant Pathology Journal
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• v.18 no.5
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• pp.259-265
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• 2002

Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

• Journal of Biosystems Engineering
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• v.43 no.3
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• pp.161-172
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• 2018

Purpose: During tillage operations, the selection of a working machine (tool) depends on the soil conditions as well as the type of tillage operation to be performed. The goal of this research was to ascertain the effects of varying working machine parameters of a compact disc harrow on tillage operations under various soil moisture content (SMC) conditions. Methods: The working machine parameters were the disc spacing and machine speed. The tillage parameters under investigation were the soil inversion ratio (SIR), tillage cutting depth (TCD), and soil clod breakage ratio (SCB). To determine the SIR, the areas of the white regions before and after tillage were obtained. The ratio of the difference of the areas of the white regions before and after tillage to the area of the white regions before tillage was considered as the SIR. The SCB was obtained as the ratio of the weight of soil clods after sieving with a mesh size of <0.02 m to the total weight of the soil clods before sieving. The soil TCD was measured using a tape measure at random points after the tillage operation. The resulting data were statistically analyzed in a one-way analysis of variance. Results: The highest soil inversion was achieved when the machine speed was 0.2 m/s with the disc spaced at 0.2 m in the 16.5% SMC. At a 0.4-m/s machine speed and 0.3-m disc spacing the highest soil breakage was achieved in the 26.5% SMC. The highest TCD was achieved at a 0.2-m/s machine speed and 0.2-m disc spacing in the 16.5% SMC. Conclusions: It was concluded that varying the working machine parameters, such as the disc spacing and machine speed, could significantly affect the soil inversion and soil clod breakage; however, it had no significant impact on the TCD.