• 한국산학기술학회논문지
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• 제20권2호
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• pp.434-442
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• 2019

도시 내 빈번히 발생하는 홍수 피해를 해결하기 위한 대응 방안으로 자연상태의 수문순환 체계를 회복하기 위한 저영향개발(Low Impact Development, LID) 기법이 대두되고 있다. LID 요소기술 중 하나인 생태저류지는 유역에서 발생하는 유출수를 저류 및 침투하여 우수 유출수 및 비점오염물질 저감효과를 갖는 시설로 다양한 연구가 진행되고 있으나, 유출저감효과에 대한 분석은 미흡한 실정이다. 따라서 본 연구에서는 수문 해석이 가능한 K-LIDM을 이용하여 소규모유역에서 생태저류지의 유출 저감효과를 분석하였다. 시나리오를 구성함에 있어 생태저류지의 저류용량을 증가시키거나 유역과 생태저류지를 분할하였고 확률강우량을 활용하여 단기강우를 모의하였다. 분석결과 저류용량 증가에 따라 20%, 분산형 시스템에서 5~15% 이상의 유출 저감효과를 나타냈으며 향후 생태저류지의 저류깊이, 지반의 침투능 및 유출부의 직경과 높이 등 다양한 시나리오에 대한 연구가 수행된다면 생태저류지의 물순환 효율성에 관하여 보다 정량적인 분석이 가능할 것으로 기대된다.

• 한국군사과학기술학회지
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• 제27권4호
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• pp.507-515
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• 2024

Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

• 한국기계가공학회지
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• 제19권11호
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• pp.78-86
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• 2020

A stress analysis was performed to verify the stability of the digging part of a garlic harvester. A finite element analysis was performed to examine the distribution and concentrated loads on the digging part of the blade and contact plate. Moreover, the stability and maximum deformation of the digging part were determined. Under a distributed load, the maximum principal stress, total deformation, and minimum safety factor ranged from 64-128 MPa, 0.35-0.70 mm, and 2.9-5.7, respectively. The analysis results for the distribution load indicated that the maximum stress occurred at the center of the blade. In contrast, under the concentrated load, the maximum principal stress, total deformation, and minimum safety factor ranged from 66-247 MPa, 0.35-0.79 mm, 1.48-5.53, respectively. The analysis results for the concentrated load indicated that stress and deformation were larger toward the edge and center, respectively.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.586-587
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• 2005

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2004년도 The 13th Korea Genome Conference
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• pp.41.2-41.2
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• 2004

• Bulletin of the Korean Chemical Society
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• 제27권10호
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• pp.1537-1541
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• 2006

The $^1H$ and $^{13}C$ chemical shifts of eleven flavone derivatives were completely determined by basic 1D and 2D NMR experiments. Nineteen flavone derivatives including the above eleven derivatives were examined for anti-oxidative effects using the 1,1-diphenyl-2-picryl-hydrazyl assay and Caenorhabditis elegans. In order to understand the relationships between the structures of flavone derivatives and their anti-oxidative activities, a Comparative Molecular Field Analysis was performed.

• The Plant Pathology Journal
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• 제37권3호
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• pp.307-314
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• 2021

Pepper (Capsicum annuum L.) is an important agricultural crop worldwide. Recently, Colletotrichum scovillei, a member of the C. acutatum species complex, was reported to be the dominant pathogen causing pepper anthracnose disease in South Korea. In the present study, we isolated bacterial strains from rhizosphere soil in a pepper field in Gangwon Province, Korea, and assessed their antifungal ability against C. scovillei strain KC05. Among these strains, a strain named BS1 significantly inhibited mycelial growth, appressorium formation, and disease development of C. scovillei. By combined sequence analysis using 16S rRNA and partial gyrA sequences, strain BS1 was identified as Bacillus velezensis, a member of the B. subtilis species complex. BS1 produced hydrolytic enzymes (cellulase and protease) and iron-chelating siderophores. It also promoted chili pepper (cv. Nockwang) seedling growth compared with untreated plants. The study concluded that B. velezensis BS1 has good potential as a biocontrol agent of anthracnose disease in chili pepper caused by C. scovillei.

• Journal of Microbiology and Biotechnology
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• 제25권5호
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• pp.704-708
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• 2015

For the analysis of multiple-reaction intermediates for long-chain dicarboxylic acid biotransformation, simple and reproducible methods of extraction and derivatization were developed on the basis of gas chromatography with flame ionization detector (GC-FID) instead of mass spectrometry. In the derivatization step, change of the ratio of pyridine to MSTFA from 1:3 to 9:1 resulted in higher peak intensity (p = 0.021) and reproducibility (0.6%CV) when analyzing 32 g/l ricinoleic acid (RA). Extraction of RA and ω-hydroxyundec-9-enoic acid with water containing 100 mM Tween 80 showed 90.4-99.9% relative extraction efficiency and 2-7%CV compared with those with hydrophobic ethyl acetate. In conclusion, reduction of the pyridine content and change of the extraction solvent to water with Tween 80 provided compatible derivatization and extraction methods to GC-FID-based analysis of longchain carboxylic acids.

• Genomics & Informatics
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• 제5권4호
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• pp.179-187
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• 2007

An increasing number of primate genomes are being sequenced. A direct comparison of repeat elements in human genes and their corresponding chimpanzee orthologs will not only give information on their evolution, but also shed light on the major evolutionary events that shaped our species. We have developed REPEATOME to enable visualization and subsequent comparisons of human and chimpanzee repeat elements. REPEATOME (http://www.repeatome.org/) provides easy access to a complete repeat element map of the human genome, as well as repeat element-associated information. It provides a convenient and effective way to access the repeat elements within or spanning the functional regions in human and chimpanzee genome sequences. REPEATOME includes information to compare repeat elements and gene structures of human genes and their counterparts in chimpanzee. This database can be accessed using comparative search options such as intersection, union, and difference to find lineage-specific or common repeat elements. REPEATOME allows researchers to perform visualization and comparative analysis of repeat elements in human and chimpanzee.

• Korean Journal of Acupuncture
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• 제28권4호
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• pp.41-48
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• 2011

Objectives : This study aimed to explore the passive multi-channel time series analysis method by measuring bio-potentials of acupuncture point and the peripheral region Methods : Bio-potential was measured at ST36 and the peripherical region of ST36 of 5 healthy volunteers at three times. The diagram of the potential changes over time were smoothed by moving average method and similarities of ST36 and the other points were calculated. Results : In the normal weight group, bio-potential similarity tended to decrease in proportion to the distance from the acupuncture point. In the obesity group, bio-potential similarity appeared in a very wide area. Bio-potential similarity had positive correlation with BMI value. Conclusions : The passive multi-channel time series analysis method showed the possibility be appropriate for the electrical characteristics study of meridians.