• Title/Summary/Keyword: Bio-analysis

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THE SKELETAL STABILITY OF LE FORT I MAXILLARY ADVANCEMENT (Le Fort I 상악골전진술 후 안정성에 관한 연구)

  • Seo, Min-Gyo;Koo, Sung-Young;Kim, Eun-Ju;Leem, Dae-Ho;Shin, Hyo-Keun;Ko, Seung-O
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.32 no.2
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    • pp.149-153
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    • 2010
  • The purpose of this retrospective study was to evaluate relapse, comparing large and small maxillary advancements with four-plate rigid fixation and without bone grafting. All patients had skeletal class III malocclusion, and underwent bimaxillary surgery. Standardized cephalometric analysis by one examiner was performed on serial radiographs of 14 patients immediately before surgery, and within 1 week and at least 6 months postoperatively (mean 10 months). The group was divided into two subsets to determine whether the magnitude of relapse. In group 1 ($\leq$ 5 mm, n = 8), the average advancement was $4.0{\pm}0.9\;mm$, with a mean relapse of $0.1{\pm}0.5\;mm$. In group 2 (6-8 mm, n = 4), the average advancement was $6.8{\pm}0.9\;mm$, with a mean relapse of $0.7{\pm}0.4\;mm$. There was no statistical difference in the measured relapse among the groups. Maxillary advancement with a 1-piece Le Fort I osteotomy is a relatively stable procedure.

Identification of a Genetic Locus Related to Antivirus Production in Pseudomonas fluorescence strain Gpf01 Against Cucumber mosaic virus

  • Cho, Sae-Youll;Lee, Seon-Hwa;Park, Su-Jin;Choi, Kyu-Up;Cho, Jun-Mo;Hur, Jang-Hyun;Shrestha, Anupama;Lim, Chun-Keun
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.77-85
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    • 2009
  • Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3 kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.

Effect of carbon substrate on the intracellular fluxes in succinic acid producing Escherichia coli.

  • Hong, Soon-Ho;Lee, Dong-Yup;Kim, Tae-Yong;Lee, Sang-Yup;Park, Sun-Won
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2003.10a
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    • pp.251-257
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    • 2003
  • Metabolic engineering has become a new paradigm for the more efficient production of desired bioproducts. Metabolic engineering can be defined as directed modification of cellular metabolism and properties through the introduction, deletion, and modification of metabolic pathways by using recombinant DNA and other molecular biological tools. During the last decade, metabolic flux analysis(MFA) has become an essential tool fur metabolic engineering. By MFA, the intracellular metabolic fluxes can be quantified by the measurement of extracellular metabolite concentrations in combination with the stoichiometry of intracellular reactions and mass balances. The usefulness and functionality of MFA are demonstrated by applying to metabolic pathways in E. coli. First, a large-scale in silico E. coli model is constructed, and then the effects of carbon sources on intracellular flux distributions and succinic acid production were investigated on the basis of the uptake and secretion rates of the relevant metabolites. The results indicated that succinic acid yields increased in order of gluconate, glucose and sorbitol. Acetic acid and lactic acid were produced as major products rather than when gluconate and glucose were used carbon sources. The results indicated that among three carbon sources available, the most reduced substrate is sorbitol which yields efficient succinic acid production.

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Comparative Study on Efficiencies of Naturally-Ventilated Multi-Span Greenhouses in Korea (다연동 온실의 자연환기효율성 비교 분석)

  • Kwon, Soon-Hong;Jung, Sung-Won;Kwon, Soon-Gu;Park, Jong-Min;Choi, Won-Sik;Kim, Jong-Soon
    • Journal of the Korean Society of Industry Convergence
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    • v.20 no.1
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    • pp.8-18
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    • 2017
  • This research analyzed the ventilation effect of the multi-span greenhouse based on the types of greenhouse structure, weather conditions, and locations inside the greenhouse. To compare and analyze the ventilation effects with different types of greenhouse, the uniform environmental conditions should be selected in advance. But these factors are not controlled and require tense many precision facilities and labor forces. Thus, the CFD simulation was used for the air stream to be analyzed qualitatively and quantitatively. In addition, for the ventilation effect analysis, the TGD (Tracer Gas Decay) was used to overcome the shortcomings of the current ventilation measurement method. The calculation error of ventilation rate using TGD was low (10.5%). Thus, the TGD is very effective in calculating the ventilation efficiency. The wind direction of 90 degrees showed the best ventilation effect. The ventilation rate also decreased along the air circulation path, and the rate was the lowest around the outlet. The computed fluid method (CFD) turned out to be a power tool for simulating flow behavior in greenhouse.

Analysis of Essential Proteins in Protein-Protein Interaction Networks (단백질 상호작용 네트워크에서 필수 단백질의 견고성 분석)

  • Ryu, Jae-Woon;Kang, Tae-Ho;Yoo, Jae-Soo;Kim, Hak-Yong
    • The Journal of the Korea Contents Association
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    • v.8 no.6
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    • pp.74-81
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    • 2008
  • Protein interaction network contains a small number of highly connected protein, denoted hub and many destitutely connected proteins. Recently, several studies described that a hub protein is more likely to be essential than a non-hub protein. This phenomenon called as a centrality-lethality rule. This nile is widely credited to exhibit the importance of hub proteins in the complex network and the significance of network architecture as well. To confirm whether the rule is accurate, we Investigated all protein interaction DBs of yeast in the public sites such as Uetz, Ito, MIPS, DIP, SGB, and BioGRID. Interestingly, the protein network shows that the rule is correct in lower scale DBs (e.g., Uetz, Ito, and DIP) but is not correct in higher scale DBs (e.g., SGD and BioGRID). We are now analyzing the features of networks obtained from the SGD and BioGRD and comparing those of network from the DIP.

Development and Assessment of Conductive Fabric Sensor for Evaluating Knee Movement using Bio-impedance Measurement Method (슬관절 운동 평가를 위한 생체 임피던스 측정용 전도성 섬유센서 개발 및 평가)

  • Lee, Byung-Woo;Lee, Chung-Keun;Cho, Ha-Kyung;Lee, Myoung-Ho
    • Journal of Biomedical Engineering Research
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    • v.32 no.1
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    • pp.37-44
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    • 2011
  • This paper describes the development and assessment of conductive fabric sensor for evaluating knee movement using bio-impedance measurement method. The proposed strip-typed conductive fabric sensor is compared with a dot-typed Ag/AgCl electrode for evaluating validity under knee movement condition. Subjects are composed of ten males($26.6{\pm}2.591$) who have not had problems on their knee. The strip-typed conductive fabric sensor is analyzed by correlation and reliability between a dot-typed Ag/AgCl electrode and the strip-typed conductive fabric sensor. The difference of bio-impedance between a dot-typed Ag/AgCl electrode and the strip-typed conductive fabric sensor averages $7.067{\pm}13.987\;{\Omega}$ As the p-value is under 0.0001 in 99% of t-distribution, the strip-typed conductive fabric sensor is correlated with a dot-typed Ag/AgCl electrode by SPSS software. The strip-typed conductive fabric sensor has reliability when it is compared with a dot-typed Ag/AgCl electrode because most of bio-impedance values are in ${\pm}1.96$ standard deviation by Bland-Altman Analysis. As a result, the strip-typed conductive fabric sensor can be used for assessing knee movement through bio-impedance measurement method as a dot-typed Ag/AgCl electrode. Futhermore, the strip-typed conductive fabric sensor is available for wearable circumstances, applications and industries in the near future.