• Title/Summary/Keyword: Bio industry

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Induction of Apoptosis by Methanol Extract of Endlicheria anomala in Human Lung and Liver Cancer Cells (Endlicheria anomala 메탄올 추출물에 의한 인체 폐암세포주와 간암세포주의 자가사멸 유도)

  • Park, Hyun-jin;Jin, Soojung;Oh, You Na;Kim, Byung Woo;Kwon, Hyun Ju
    • Journal of Life Science
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    • v.25 no.4
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    • pp.441-449
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    • 2015
  • Endlicheria anomala, a neotropical plant, is found in northern South America and the Amazon region. It is traditionally used to remove poisons and cure gangrene. According to recent data, this plant has diverse biological properties such as anti-oxidative, anti-inflammatory and anti-melanogenic properties. However, the anti-cancer effect of E. anomala and its molecular mechanisms remain unclear. In this study, we examined the anti-cancer effect and the active mechanism of methanol extract of E. anomala (MEEA) in human lung adenocarcinoma cells (A549) and human liver cancer cells (HepG2). Our data revealed that MEEA showed cytotoxic activity in a dose-dependent manner and induced apoptosis both in A549 and HepG2 cells. We verified evidences of apoptosis via formation of chromatin condensation, apoptotic body and accumulation of cells in the subG1 phase. Following observed apoptosis-related phenomena, we found that the induction of apoptosis by MEEA was associated with the increase of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) expression. Furthermore, MEEA-induced apoptosis was characterized with proteolytic activation of caspase-3, degradation of poly ADP ribose polymerase (PARP), and up-regulation of pro-apoptotic Bax expression. Taken together, these findings indicate that MEEA may have potential cancer therapeutic utility in A549 and HepG2 cells.

Optimization of Cultivation and Storage Conditions on Red Cabbage Seed Sprouts (적양배추 새싹채소의 발아 및 저장 조건 최적화)

  • Baek, Kyeong-Hwan;Jo, Doekjo;Yoon, Sung-Ran;Kim, Gui-Ran;Park, Ju-Hwan;Lee, Gee-Dong;Kim, Jeong-Sook;Kim, Yuri;Han, Bumsoo;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.13-19
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    • 2013
  • This study was carried out to find the optimal conditions for red cabbage seed sprouts in terms of their physicochemical and sensory qualities by electron-beam irradiation, cultivation and storage using the response surface methodology (RSM). Moisture content ($R^2$=0.9638) was affected by irradiation dose and cultivation time. Total phenolics content ($R^2$=0.9117) was mainly affected by irradiation dose, but carotenoid content ($R^2$=0.8338) was affected in the order of irradiation dose, cultivation time and storage time. Sensory properties were also affected by irradiation dose, and thus scores decreased as irradiation dose increased. The optimum conditions estimated by superimposing total phenolics content and overall acceptance were 2.2-3.8 kGy of the irradiation dose, 3.0-4.0 days of cultivation and 2.0-3.0 days of storage.

Diet Composition of Skinnycheek Lanternfish, Benthosema pterotum in the Coastal Waters of South Sea, Korea (한국 남해에 출현하는 깃비늘치(Benthosema pterotum)의 위내용물 조성)

  • Suyeon Jin;Gi Chang Seong;Do-Gyun Kim;Da Yeon Kang;Hyeon Ji Kim;Gun Wook Baeck
    • Korean Journal of Ichthyology
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    • v.35 no.2
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    • pp.129-135
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    • 2023
  • The diet composition of skinnycheek lanternfish, Benthosema pterotum were collected by bottom trawl net in the South Sea of Korea during 2021 (February, May, August, and November) and 2022 (February, April, August, and November). The size of the specimens ranged from 16 to 73 mm in total length. B. pterotum were mesopelagic carnivores that mainly consumed euphausiids and copepods. Its diets included small quantities of amphipods, shrimps, mysids, etc. We calculated the trophic level as 3.16 for B. pterotum. The individuals of small size class (<40 mm) fed mainly on copepods. In the next size classes (40~45 mm, 45~50 mm, 50~55 mm), copepods' proportion decreased and euphausiids increased. In the largest class (55~60 mm and ≥60 mm), the proportion of euphausiids' decreased, and shrimps increased. As the body size of B. pterotum increased the mean weight of prey per stomach (mW/ST) tended to significantly increase (One-way ANOVA, P<0.05).

Feeding Habits of Small Yellow Croaker, Larimichthys polyactis in Coastal Waters of Korea (한국 연안해역에 출현하는 참조기(Larimichthys polyactis)의 식성)

  • Kang, Da Yeon;Seong, Gi Chang;Kim, Do-Gyun;Jin, Suyeon;Soh, Ho Young;Baeck, Gun Wook
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.201-207
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    • 2022
  • Total 418 Yellow Croaker Larimichthys polyactis were examined and the range of total length (TL) was 12.0~27.5 cm and average total length was 19.6 cm. The most important prey component in the diets of L. polyactis was Pisces that constituted 46.5% in %IRI (Index of relative importance). Euphausiacea was the second largest prey component. The result of analysis in ontogenetic and seasonal changes significantly exhibited. The proportion of Pisces increased as increasing body size, whereas the consumption of Macrura decreased gradually. The diets also were different among seasons, with the difference particularly being evident between warm (summer and autumn) and cold (winter and spring) seasons. Permutational multivariate analysis of variance (PERMANOVA) revealed significant dietary differences by seasons (P=0.001).

Feeding Habits of Whitespotted Conger, Conger myriaster in the West Sea of Korea (한국 서해에 출현하는 붕장어(Conger myriaster)의 식성)

  • Da Yeon Kang;Do-Gyun Kim;Gi Chang Seong;Suyeon Jin;Jae Mook Jeong;Su Kyung Kang;Gun Wook Baeck
    • Korean Journal of Ichthyology
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    • v.35 no.3
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    • pp.177-182
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    • 2023
  • Total 158 Conger myriaster were examined and the range of Preanal length (PaL) was 8.2~40.1 cm and average Preanal length was 17.3 cm. Proportion of the empty stomach was 30.4% and individuals, which the prey items were found in stomach, were 110. The most important prey component in the diets of C. myriaster was Pisces that constituted 54.4% in %IRI (Index of relative importance). Engraulis japonicus was the most important prey component in Pisces. Macrura was the second largest prey component and Crangon hakodatei was the most important prey component in Macrura. The result of analysis in ontogenetic changes significantly exhibited among three size classes (<15.0 cm, 15.0~20.0 cm, ≥20.0 cm). The proportion of Macrura decreased as increasing body size, whereas the consumption of Pisces increased gradually. As body size of C. myriaster increased, the mean weight of prey per stomach (mW/ST) increased (one way-ANOVA, P<0.05).

Property about Extraction of Metal Ion in the Synthesized Crown Ether Model Compounds (합성한 crown ether 모델 화합물에서 금속이온의 추출 특성 연구)

  • Lee, Yong-Hee;Suh, Myung-Gyo;Roh, Jong-Su;Lee, Kook-Eui;Lee, Young-Sei
    • Journal of the Korean Society of Industry Convergence
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    • v.6 no.3
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    • pp.165-169
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    • 2003
  • To extract alkali metal ions and heavy metal ions, search for crown ether model compounds (4a-b, 5a-b, 6a-b) bearing side arm has led to achieve in 5~6 steps starting from 2,6-dimethylaniline. The determination of structure in their compound derivatives were on the basis of melting point and nuclear magnetic resonance spectroscopy. In the solvent extraction of metal ions from the synthesized derivatives, we observed that silver ion has only high selectivity for synergistic ligation of crown ether.

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Comparative Study on Efficiencies of Naturally-Ventilated Multi-Span Greenhouses in Korea (다연동 온실의 자연환기효율성 비교 분석)

  • Kwon, Soon-Hong;Jung, Sung-Won;Kwon, Soon-Gu;Park, Jong-Min;Choi, Won-Sik;Kim, Jong-Soon
    • Journal of the Korean Society of Industry Convergence
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    • v.20 no.1
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    • pp.8-18
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    • 2017
  • This research analyzed the ventilation effect of the multi-span greenhouse based on the types of greenhouse structure, weather conditions, and locations inside the greenhouse. To compare and analyze the ventilation effects with different types of greenhouse, the uniform environmental conditions should be selected in advance. But these factors are not controlled and require tense many precision facilities and labor forces. Thus, the CFD simulation was used for the air stream to be analyzed qualitatively and quantitatively. In addition, for the ventilation effect analysis, the TGD (Tracer Gas Decay) was used to overcome the shortcomings of the current ventilation measurement method. The calculation error of ventilation rate using TGD was low (10.5%). Thus, the TGD is very effective in calculating the ventilation efficiency. The wind direction of 90 degrees showed the best ventilation effect. The ventilation rate also decreased along the air circulation path, and the rate was the lowest around the outlet. The computed fluid method (CFD) turned out to be a power tool for simulating flow behavior in greenhouse.

Sprinkler Flow Rate Effect on the Greenhouse System for Soilless Cultivation of Cucumber (오이재배의 비닐하우스 시스템에서 스프링클러의 유량효과)

  • Yun, Sang-Jin;Chung, Sung-Won;Park, Jong-Min;Kim, Jong-Soon;Kwon, Soon-Goo;Choi, Won-Sik
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.1
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    • pp.93-100
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    • 2020
  • In this paper, soilless cucumber cultivation was investigated, fermented fertilizer to increase the cucumber yield was considered, and the greenhouse temperature control system for cucumber cultivation is developed. To do this, perlite was proposed to replace soil as growth medium. Fermented cows and pigs manure was proposed as plant fertilizer. Combination of fan, water sprinkler, and pipe heating system was proposed to control the greenhouse temperature. However, because this research was conducted during the summer, the greenhouse system observed in this study only focused on variations in the flow rate of the water sprinkles used. The experimental result shows that soilless culture in the greenhouse could be an alternative to traditional field production for high-value vegetable crops. Furthermore, application of fermented fertilizer of 10% could enhance the growth and increase the yield and quality of crops. The proposed sprinkler flow rate is best suited for cucumber crop with the best thrives was 0.846 kg/s.

Pressure Drop Characteristics of Air Particle Flow in Powder Transport Piping System (파우더 수송시스템의 공기입자 유동 압력강하 특성)

  • Kim, Jong-Soon;Chung, Sung-Won;Kwon, Soon-Gu;Park, Jong-Min;Choi, Won-Sik;Kwon, Soon-Hong
    • Journal of the Korean Society of Industry Convergence
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    • v.20 no.2
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    • pp.157-168
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    • 2017
  • The pressure drop characteristics of air particle flow in a powder transport piping system were analyzed in this study. The pressure drop characteristics of air particle flow in the piping system have not well understood due to the complexibility of particle motion mechanism. Particles or powders suspended in the air flow cause the increase of the pressure drop and affect directly transport efficiency. In this study, the pressure drop in a powder transport piping system was analyzed with interactions of air flow and particle motion in straight and curved pipes. The total pressure drop increased with pipe length, mixture ratio, and friction factor of particles because of increased friction loss of air and particles in the piping system. For the coal powders of $74{\mu}msize$ and powder-to-air mass mixture ratio of 0.667, the total pressure drop under the consideration of powders and air flow was calculated as much as 30% higher than that air flow only.