• 제목/요약/키워드: Binding Interface

검색결과 103건 처리시간 0.025초

프록시 바인딩 갱신을 이용한 수직적 핸드오버 기법 (A Vertical Handover Scheme Using Proxy Binding Update)

  • 박희동
    • 한국인터넷방송통신학회논문지
    • /
    • 제10권1호
    • /
    • pp.15-20
    • /
    • 2010
  • 본 논문은 멀티 호밍 기반의 프록시 바인딩 갱신 (Proxy binding update)을 이용하여 이동 노드가 다른 종류의 무선 액세스 망으로 이동할 때 발생하는 수직적 핸드오버 (Vertical handover) 지연 및 그에 따른 패킷 손실을 최소화하는 방안을 제안하였다. 제안 방안에서 다중 인터페이스를 가지는 이동 단말이 이종 액세스 망으로 이동할 경우 기존의 인터페이스를 통한 네트워크 연결을 유지한 채 새로운 인터페이스를 이용해 미리 3계층 핸드오버를 수행함으로써 빠르고 끊김 없는 핸드오버가 가능하다. 제안된 프록시 바인딩 갱신이 Mobile IP의 바인딩 갱신과 다른 점은 바인딩 갱신 메시지에 자신의 인터페이스가 아닌 이전 액세스 망과 통신하고 있는 인터페이스의 홈 주소 (Home address)를 사용한다는 점이다. 성능 분석 결과 제안된 수직적 핸드오버 기법이 핸드오버 지연 및 패킷 손실을 효율적으로 감소시킬 수 있음을 확인할 수 있었다.

Backbone assignment of HMGB1 A-box and molecular interaction with Hoxc9DBD studied by paramagnetic probe

  • Choi, Ji Woong;Park, Sung Jean
    • 한국자기공명학회논문지
    • /
    • 제25권2호
    • /
    • pp.17-23
    • /
    • 2021
  • High mobility group protein B1 (HMGB1) is a highly conserved, non-histone, chromatin associated nuclear protein encoded by HMGB1 gene. HMGB1 proteins may be general co-factors in Hox-mediated transcriptional activation that facilitate the access of Hox proteins to specific DNA targets. It is unclear that the exact binding interface of Hoxc9DBD and HMGB1. To identify the interface and binding affinity of Hoxc9DBD and HMGB1 A-box, the paramagnetic probe, MTSL was used in NMR titration experiment. It is attached to the N-terminal end of HMGB1 A-box by reaction with thiol groups. The backbone assignment of HMGB1 A-box was achieved with 3D NMR techinques. The 15N-labeled HMGB1 A-box was titrated with MTSL-labeled Hoxc9DBD respectively. Based on the chemical shift changes we can identify the interacting residues and further map out the binding sites on the protein structure. The NMR titration result showed that the binding interface of HMGB1 A-box is around loop-1 between helix-1 and helix-2. In addition, the additional contacts were found in N- and C-terminus. The N-terminal arm region of Hoxc9DBD is the major binding region and the loop between helix1 and helix2 is the minor binding region.

Effects of Hin Recombinase Dimer Interface Mutants on DNA Binding and Recombination

  • Lee, Hee-Jung;Lee, Sun-Young;Lee, Hee-Jin;Lim, Heon-Man
    • 한국미생물생명공학회:학술대회논문집
    • /
    • 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
    • /
    • pp.27-31
    • /
    • 2001
  • Previous biochemical assays and a structural model indicated that the dimer interface of the Hin recombinase is composed of the two a-helices. To elucidate the structure and function of the helix, amino acids in the N-terminal end of the helix, where the two helices contact most, were randomized, and inversion-incompetent mutants were selected. To investigate why the mutants lost their inversion activities, the DNA binding, hix-pairing, invertasome formation, and DNA cleavage activities were assayed using in vivo and in vitro methodologies. Results indicated that the mutants could be divided into 4 classes based on their DNA binding activity. We proposed that the a-helices might place a DNA binding motif of Hin properly to the minor DNA groove of the recombination site. All the mutants except the non-binders were able to perform hix-pairing and invertasome formation, suggesting that the dimer interface is not involved in the process of hix-pairing or invertasome formation. The inversion-incompetent phenotype of the binders was caused by the inability of mutants to perform the DNA cleavage activity. The less binders exhibited wild-type level of hix-pairing activity because the hix-pairing activity overcomes the DNA binding defect of the less binders. This phenotype of the less binders suggests that the binding domains of Hin could mediate Hin-Hin interaction during hix-pairing..

  • PDF

C++ 객체 영속성 부여를 위한 이음새 없는 인터페이스의 설계 및 구현 (Design and Implementation of Seamless Interface Providing Persistence to C++ Object)

  • 이미영;김명준
    • 한국정보과학회논문지:컴퓨팅의 실제 및 레터
    • /
    • 제6권5호
    • /
    • pp.468-476
    • /
    • 2000
  • 객체지향 언어 C++ 와 데이터베이스 관리 시스템의 결합은 C++ 응용 프로그램의 객체들이 프로그램이 종료되어도 지속되도록 데이터베이스의 저장 기능을 이용함과 동시에 데이터베이스 시스템에서 제공하는 다양한 기능을 사용하면서, 객체에 대한 조작은 C++의 일반 임시 객체처럼 C++에서 제공하는 기능들을 이용할 수 있도록 해 준다. 본 논문에서는 C++ 응용 프로그램의 객체에 영속성을 부여하는 방법으로 객체지향 데이터베이스 시스템의 상용 표준안인 ODMG-97의 C++ 바인딩에서 제시한 인터페이스를 보완하여 영속성 부여 인터페이스를 제시하고 이를 지원하기 위한 시스템의 설계 및 구현 내용을 서술한다. 제시한 인터페이스는 영속 가능한 클래스의 모든 객체는 동일한 인터페이스를 이용하여 사용자가 원하는 대로 임시 객체, 영속 객체를 모두 생성할 수 있고, 생성되는 객체의 클래스명을 추가로 명시하지 않고도 영속 객체를 생성할 수 있게 함으로써 이음새 없는 영속성 부여 인터페이스를 제공하고, 또한 객체 생성시 데이터베이스 객체와 메모리 객체간의 타입 호환성을 보장한다.

  • PDF

IL-l/Toll-like Receptor Superfamily 신호전달에 관여하는 TRAF6 결합 Motif의 규명 (Identification of TRAF6-Binding Motif in IL-1/Toll-like Receptor Superfamily-Mediated Signal Transduction)

  • 임미정
    • 약학회지
    • /
    • 제47권3호
    • /
    • pp.180-183
    • /
    • 2003
  • Crystal structure of TRAF6 in complex with TRAF6-binding sites from CD40 was previously determined. The structure revealed a distinct TRAF6-binding groove of CD40, the key structural determinant of interaction. The structural information leads to a proposed TRAF6-binding motif. This allows the identification of TRAF6-binding sequences in the hIRAK protein, whose functional requirement in IL-1/Toll-like receptor superfamilies-mediated signal transduction is further demonstrated using site-directed mutagenesis. The mutational effects of hIRAK on the down-stream NF-kB signaling shows the importance of the TRAF6 interface for signaling by IL-1/Toll-like receptor superfamilies.

압력잠김 및 열고착 현상 발생가능 밸브의 선정 (Selection of Valves Susceptible to Pressure Locking and Thermal Binding)

  • 이성노;안진근;김석범
    • 한국유체기계학회 논문집
    • /
    • 제10권5호
    • /
    • pp.20-26
    • /
    • 2007
  • Some gate valves are susceptible to pressure locking and thermal binding which prevent the safety function. The safety related gate valves susceptible to pressure locking and thermal binding shall be identified and taken preventive actions to ensure the safety function. The identification of the gate valves susceptible to pressure locking and thermal binding needs the evaluation of system design, valve and piping arrangement, test requirements, and operating conditions. Application of preventive methods should consider the system safety function, applicability, effectiveness, interface with system design, and cost. The selection procedure of valves susceptible to pressure locking and thermal binding can be effectively used in industry including nuclear power plants. In order to prevent the pressure locking, the hole can be drilled through the one disc of upstream side or down stream and the external equalizing line can be installed from bonnet to downstream or upstream. The double disc parallel seat valve type can be used instead of flexible wedge gate valve to prevent the thermal binding. The identification of gate valves susceptible to pressure locking and thermal binding, and preventive actions will meet the regulatory requirements and enhance the availability and safety of plants.

Functional Importance of TRAF6-Binding Motif in IL -1 Mediated Signal Transduction

  • Yim , Mi-Jung
    • 대한약학회:학술대회논문집
    • /
    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
    • /
    • pp.311.1-311.1
    • /
    • 2002
  • Crystal structure of TRAF6 in complex with TRAF6-binding sites from CD40 was previously determined. The structure revealed a distinct TRAF6-binding groove of CD40. the key structural determinant of interaction. The structural information leads to a proposed TRAF6-binding motif. This allows the identification of TRAF6-binding sequences in the hlRAK protein, whose functional requirement in IL-1 mediated signal transduction is further demonstrated using site-directed mutagenesis. The mutational in IL-1 mediated signal transduction is further dimonstrated using site-directed mutagenesis. The mutational effects of hlRAK on the down-stream NF-${\kappa}$ signaling shows the importance of the TRAF6 interface for signaling by IL-1.

  • PDF

고농도 염화물을 함유하는 혼합시멘트 계에서 철근부식에 따른 미세구조의 변화와 전기화학적 특성 (Microstructure modification and electrochemical properties of steel corrosion in the blended cement systems containing internal chlorides)

  • 나종윤;이승헌;김창은
    • 한국콘크리트학회:학술대회논문집
    • /
    • 한국콘크리트학회 1999년도 학회창립 10주년 기념 1999년도 가을 학술발표회 논문집
    • /
    • pp.131-134
    • /
    • 1999
  • Microstructure modification and electrochemical properties are investigate to estimate the effects of internal chlorides on the steel corrosion in the blended cement systems. According to the test results, slag cement system showed high chloride binding capacity and low corrosion rate. The impedance data showed three distince arcs from lowest(mHz) frequency to highest (MHz) frequency due to product layer, interfacial reaction and bulk matrix. Through the microstructural investigation, fine steel-matrix interface of slag cement system was observed but rough steel-matrix interface of OPC system was observed. Friedel's salt was thought that the substantial material contributed to the chloride binding of slag cement system.

  • PDF

구리와 코디에라이트와의 접촉점에서 구리에 대한 ESCA 스펙트럼의 에너지 교정 (Energy Calibration of ESCA Spectrum for the Copper in the Interface of Copper and Cordierite)

  • Han, Byoung-Sung
    • 대한전자공학회논문지
    • /
    • 제25권1호
    • /
    • pp.27-32
    • /
    • 1988
  • Electron Spectroscopy for Chemical Analysis(ESCA) allowes the determination of the elemental composition and the bonding state of the surface atomes in the interface between two materials. In the binding energies of ESCA spectrum, there are zero error, voltage scaling error and random error. Accurate analysis of the intensity energy response functions and accurate calibration of the energy scale are essential to use X-ray photoelectron spectron meter. At the results of the calibration of the ESCA spectra in the copper and cordierite (Mg2Al4Si5kO18) interfaces, the errors relative to the copper are -3.03 eV for the zero error -z,-197 ppm for the voltage scaling error -V and 6.9 meV for the random error -R. The method of the calibration is able to apply for the binding energy calibration of the another ESCA spectra.

  • PDF

Factors affecting the chloride threshold level for steel corrosion in concrete

  • Moon, Han-Young;Ann, Ki-Yong;Kim, Hong-Sam
    • 한국콘크리트학회:학술대회논문집
    • /
    • 한국콘크리트학회 2005년도 봄학술 발표회 논문집(II)
    • /
    • pp.205-208
    • /
    • 2005
  • The present study surveys the concrete properties-concerned factors influencing the chloride threshold level for steel corrosion in concrete, altogether with supporting experimental works, in particular, chloride binding capacity, buffering capacity, condition of steel-concrete interface and cement replacement. It concluded that the order of the dominance on CTL is such that chloride binding < buffering capacity of cement matrix < physical condition of steel-concrete interface. This is attributed to the fact that calcium hydroxide does not form a continuous layer on the steel surface and that bound chlorides are released at the stage of corrosion initiation.

  • PDF