• Title/Summary/Keyword: Binding Capacity

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Chloride binding isotherms of various cements basing on binding capacity of hydrates

  • Tran, Van Mien;Nawa, Toyoharu;Stitmannaithum, Boonchai
    • Computers and Concrete
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    • v.13 no.6
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    • pp.695-707
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    • 2014
  • This study investigated the chloride binding isotherms of various cement types, especially the contributions of C-S-H and AFm hydrates to the chloride binding isotherms were determined. Ordinary Portland cement (OPC), Modified cement (MC), Rapid-hardening Portland cement (RHC) and Low-heat Portland cement (LHC) were used. The total chloride contents and free chloride contents were analyzed by ASTM. The contents of C-S-H, AFm hydrates and Friedel's salt were determined by X-ray diffraction Rietveld (XRD Rietveld) analysis. The results showed that OPC had the highest chloride binding capacity, and, LHC had the lowest binding capacity of chloride ions. MC and RHC had very similar capacities to bind chloride ions. Experimental equations which distinguish the chemically bound chloride and physically bound chloride were formulated to determine amounts of the bound chloride basing on chloride binding capacity of hydrates.

Binding Capacity of Human Serum Albumin with Estrogen and Other Ligands (Human Serum Albumin이 Estrogen과 기타 Ligands와의 결합력에 관한 연구)

  • Park, Geum-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.3
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    • pp.414-419
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    • 1994
  • This study was trying to find what physical changes occurred to albumin when it reacted with estrogen and other ligands. Each concentration of human serum albumin with 100$\mu$l estradiol reacted at the highest binding capacity of 280nm. In addition, 1 hr of reaction time showed the highest binding rate. Conformational changes in human serum albumin with dietylstillbesterol and N-ethyl-maleimide produced strong binding capacities. The changes were immediate and they did not increase or decrease over time. Effects of human serum albumin with estriol induced no interaction each other. The binding capacity of human serum albumin with vitamin D$_2$was lower than estradiol. and the highest binding rate showed 1 hr of reaction time. Vitamin D$_2$ was very similar to the binding capacity of estradiol.

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Binding Capacity of Chitin and Chitosan to Anthocyanin Pigment Isolated from Purple Perilla Leaves

  • Chang, Eun-Ju;Park, Sang-Won;No, Hong-Kyoon
    • Preventive Nutrition and Food Science
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    • v.5 no.1
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    • pp.1-6
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    • 2000
  • The binding capacity of chitin and chitosan to anthocyanin pigmentss isolated from purple perilla leaves was inves-tigated. The pigment binding capacity increased with increasing pigment concentrations and decreasing pH without being affected by reaction temperature and particle sizes. Regression analysis revealed significantly high corre-lations between pigment binding capacity of chitin and chitosan and pigment concentration at ranges of 25-100 mg of pigment/g of sample, After 1 hr settling, release of pigment from pigmented chitin and chitosan increased with increasing pH, up to 24.9% and 17.4%, respectively, at pH 9. In general, pigment binding capacity of chitosan was higher than that of chitin. There results suggest that chitosan may be useful as a potential adsorbent capable of stabilizing anthocyanin pigment.

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Effects of Waterborne Iron on Serum Iron Concentration and Iron Binding Capacity of Olive Flounder (Paralichthys olivaceus)

  • Jee Jung-Hoon;Kim Seong-Gil;Kang Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.7 no.1
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    • pp.23-28
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    • 2004
  • Olive flounder (Paralichthys olivaceus) was exposed to waterborne iron (0.1, 0.5, 1, 5 and 10 mg/L) for 50 days. The effects of iron on blood iron status and iron binding capacity were studied. The serum iron concentration was significantly higher than in the group exposed to iron (1, 5 and 10 mg/L) in comparison to the control after 30 days of exposure to iron. A significant decrease in unsaturated iron binding capacity was found between the control and the group exposed to iron (5 and 10 mg/L, respectively) at 40 and 50 days, respectively. The total iron binding capacity of serum in the fish exposed to iron concentrations (5 and 10 mg/L) showed a significant decrease compared to that of the control at 40 days after iron exposure. Serum iron saturation values increased in the flounder exposed to iron concentration (5 and 10 mg/L) at 50 days. Our data suggest that sub-lethal exposure of waterborne iron alters the blood iron concentration and iron binding capacity, and these parameters seems to be valuable factors for screening and diagnosis of iron overload syndromes in fish.

The Effect of Physicochemical Properties of Salicylate Analogs on Binding to Bovine Serum Albumin (살리실산 유사체류의 물성이 우혈청 알부민 결합에 미치는 영향)

  • Yong, Chul-Soon
    • Journal of Pharmaceutical Investigation
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    • v.23 no.3
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    • pp.119-125
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    • 1993
  • The protein binding of salicylate analogs has been investigated by equilibrium dialysis. A series of binding experiments were performed in order to elucidate the effects of physicochemical properties of salicylate analogs on the binding with bovine serum albumin. Attempts to correlate affinity constants with capacity factor, steric factor and Hammett ${\sigma}$ values suggested hydrophobic forces to be involved in the binding of salicylate analogs. Steric factor contributes to binding process partly, whereas electronic interaction appears to be insignificant.

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Binding of Iron and Zinc by Vegetable Fiber (식이섬유의 무기질 결합력에 관한 연구)

  • 계수경
    • The Korean Journal of Food And Nutrition
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    • v.8 no.3
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    • pp.230-238
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    • 1995
  • Iron and zinc were bound by neutral detergent fiber (NDF) obtained from 15 kinds of vegetables being consumed commonly in Korea. Binding capacity of Fe and Zn of NDF ranged from 37.8% to 85.5% and from 81% to 25.5%, respectively showing higher binding capacity of Fe to NDF than of Zn. Both Fe and Zn binding capacity of NDF increased as pH increased and reached to a maximum at pH 7 In all vegetables. The amount of mineral (Fe and Zn) bound to NDF increased as mineral concentration Increased.

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Binding Set Analysis for Interaction of Human Serum Albumin with Cethyl Trimethylammonium Bromide

  • Bordbar, Abdol-Khalegh;Sohrabi, Nasrin;Gharibi, Hossain
    • Bulletin of the Korean Chemical Society
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    • v.25 no.6
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    • pp.791-795
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    • 2004
  • The binding of cethyl trimethylammonium bromide, (CTAB) with human serum albumin (HSA) has been investigated at 5 mM phosphate buffer pH 7.0, 27 $^{\circ}C$ and various ionic strength using ion selective membrane electrodes. This method is faster and much more accurate than equilibrium dialysis technique, so provides sufficient and accurate data for binding data analysis. A novel and simple method was introduced for resolution and characterization of binding sets on basis of binding capacity concept. The values of Hill binding parameters were estimated for each set and used for calculation of intrinsic binding affinity. The results interpreted on basis of nature of forces which interfered in the interaction and represent the existence of three and two binding sets for binding of CTAB at $10^{-4}$ and $10^{-3}$ M of NaBr, respectively.

Water Binding Capacity of Vegetable Fiber (식이섬유의 수분결합력에 관한 연구)

  • 계수경
    • The Korean Journal of Food And Nutrition
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    • v.9 no.3
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    • pp.231-235
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    • 1996
  • The water binding capacity(WC) of acid detergent fiber(ADF) was estimated. The WBC of raw vegetables ranged from 5. 1g to 24.7g water/g ADF. WBC was high in pepers and low in welsh onions. The correlation coefficients between WBC and fiber components were examined to fled which component is responsible for the deteruuning ability to bind water. The correlation coefficient between WBC and cellulose was +0.8. The binding capacity of water by fiber was affected positively by cellulose. fermentation increased in WBC of ADF. Changes of no in accordance with pH changes were evaluated at pH 2, 5.2 and 6. In all cases, WBC was high In weak acid and neutral.

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A Study on Chloride Binding Capacity of Various Blended Concretes at Early Age (초기재령에서 각종 혼합콘크리트의 염소이온 고정화능력에 관한 연구)

  • Song, Ha-Won;Lee, Chang-Hong;Lee, Kewn-Chu
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.12 no.5
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    • pp.133-142
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    • 2008
  • This paper studies the early-aged chloride binding capacity of various blended concretes including OPC(ordinary Portland cement), PFA(pulversied fly ash), GGBFS(ground granulated blast furnace slag) and SF(silica fume) cement paste. Cement pastes with 0.4 of a free water/binder ratio were cast with chloride admixed in mixing water, which ranged from 0.1 to 3.0% by weight of cement and different replacement ratios for the PFA, GGBFS and SF were used. The content of chloride in each paste was measured using water extraction method after 7 days curing. It was found that the chloride binding capacity strongly depends on binder type, replacement ratio and total chloride content. An increase in total chloride results in a decrease in the chloride binding, because of the restriction of the binding capacity of cement matrix. For the pastes containing maximum level of PFA(30%) and GGBFS(60%) replacement in this study, the chloride binding capacity was lower than those of OPC paste, and an increase in SF resulted in decreased chloride binding, which are ascribed to a latent hydration of pozzolanic materials and a fall in the pH of the pore solution, respectively. The chloride binding capacity at 7 days shows that the order of the resistance to chloride-induced corrosion is 30%PFA > 10%SF > 60%GGBFS > OPC, when chlorides are internally intruded in concrete. In addition, it is found that the binding behaviour of all binders are well described by both the Langmuir and Freundlich isotherms.

Effect of hCG on TeBG (hCG가 TeBG에 미치는 영향)

  • Sung, Ho-Kyung;Kim, Woo-Gyeum
    • The Korean Journal of Physiology
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    • v.14 no.1
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    • pp.7-13
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    • 1980
  • In the previous experiment, authors have shown that during the latter half of estrous cycle there was an increase in plasma testosterone level in the rats stimulated with hCG. To determine the physiologic significance of elevated plasma testosterone, changes of the plasma concentrations of TeBG and testosterone following hCG stimulation were analyzed in the rats having a regular 5 day cycle. The rats were divided into three groups; the control, the rats stimulated with single hCG on the day of proestrus and stimulated with hCG throughout the entire cycle. Blood samples were obtained once a day for an estrous cycle and analyzed for the binding capacity of TeBG using ammonium sulphate precipitation method and testosterone concentration by means of radioimmunoassay. Followings were the results; 1) There was no significant variation in the binding capacity of TeBG in peripheral blood during the estrous cycle of the control rats. 2) No cyclic variation in the binding capacity of TeBG was observed in the rats stimulated with single hCG on proestrus. although the levels tended to be higher in the rats with stimulation than in the control rats. 3) Continual stimulation of hCG produced a marked increase in the binding capacity of TeBG especially on the day of metaestrus. 4) The changes in the plasma level of testosterone followed the same basic pattern seen in the TeBG binding capacity. 5) From above results, the followings were suggested. a. hCG related increase of the binding capacity of TeBG is probably secondary to a modest increase in estrogen as well. b. hCG related increase of plasma testosterone in female rats is not entirely due to excess production rather in part due to decreased metabolism induced by the rise in TeBG. c. It seems likely that most of elevated testosterone shown in the rat stimulated with hCG is bound to TeBG and only small portion is unbound form which influence cellular activity. It is rather possible that an increase in TeBG could augment estrogen activity.

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