• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.329-334
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• 2006

Adipocyte-specific secretory factor (ADSF)/resistin, a hormone, is a small cysteine-rich protein secreted from adipose tissue and has been implicated in modulating adipogenesis in humans and rodents. The objective of this study was to clone a gene encoding ADSF/resistin and to characterize its function in Korean Native Cattle (Hanwoo). The coding sequence was 330 base pairs and it encoded a protein of 109 amino acids. An NCBI BLAST-search revealed the cloned cDNA fragment shared significant homology (82%) with the cDNA encoding the human ADSF/resistin. The nucleotide sequence homology of the Hanwoo sequence was 73% and 64% for the rat and mouse, respectively. A 654 bp ADSF/resistin gene promoter was cloned and putative binding sites of transcription factors were identified. Tissue distribution of ADSF mRNA was examined in liver, skeletal muscles (tenderloin, biceps femoris), subcutaneous fat, and perirenal fat by RT-PCR. ADSF mRNAs were detected in fat tissues but not in liver and muscles, suggesting that ADSF/resistin expression may be induced during adipogenesis. Although, the physiological function of ADSF/resistin in the cow remains to be determined, these data indicate ADSF is related to the adipocyte phenotype and may have a possibly regulatory role in adipocyte function.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.4
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• pp.458-462
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• 2005

The study was aimed at detecting polymorphism of the fifth intron in lipoprotein lipase (LPL) gene and analyzing association between the polymorphism and productive traits. A pair of primers was designed for amplifying the fifth intron. Sequence analysis indicated that a G1171C substitution existed in Large White breed. The mutation was detected by PCR-AfaI-RFLP. Polymorphism analysis in a pig resource family showed that there existed significant effects on carcass and meat quality traits. Thoraxwaist fat thickness of BB genotype was significantly higher (14.2%, p<0.05) than that of AA on carcass traits, while BB genotype was significantly lower (3.6% p<0.01, 4.1% p<0.01; 2.3% p<0.01, 1.9% p<0.01; 1.8% p<0.01, 1.4% p<0.05) than AA and AB genotype in pH of m. Longissimus Dorsi (LD), m. Biceps Femoris (BF), m. Semipinali Capitis (SC). The allelic frequencies were also significantly different between indigenous Chinese breeds and exotic breeds. Data analyzed revealed that the mutation locus affected production traits mostly by additive effects. Based on these results, it is necessary to do more studies on LPL gene before making the LPL locus into the application of marker-assisted selection (MAS) programs.

• Journal of Animal Science and Technology
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• v.60 no.9
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• pp.22.1-22.7
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• 2018

Background: Cooking temperature and consequently doneness of beef muscles are most important for the palatability and consumer acceptability. Current study assessed the response of mechanical texture of Hanwoo muscles as a function of cooking temperature at different ageing days. Six muscles (Psoas major (PM), Longissimus thoracics (LT), Gluteus medius (GM), Semimembranosus (SM), Biceps femoris (BF) and Triceps brachii (TB)) were collected from each 10 Hanwoo steers. Warner-Bratzler WB-shear force (WBSF) and texture profile analysis (TPA) texture profiles were determined after 3 or 21 days of chiller, and randomly assigned to four groups; non-cooked, cooked at 55, 70 or $85^{\circ}C$. Results: Toughness of WBSF and TPA hardness of Hanwoo muscles were presence in the order of LT = PM = GM = SM < BF = TB (p < 0.001) for non-cooked raw muscle, and PM < LT = GM = SM < TB=BF (p < 0.001) for cooked meat aged for 3 days. WBSF linearly increased in 3 days aged meats after cooked at a higher temperature (P < 0.05). On the other hand, toughening of the muscles were significantly (P < 0.05) differed at various temperature when muscles were aged for 21 days. WBSF of PM and LT muscles were significantly increased at a higher cooking temperature, while other muscles (i.e., GM, SM, BF, TB) showed the lowest values at $70^{\circ}C$. In the case of TPA hardness, the effect of cooking temperature was very less in the toughness of the muscle (P > 0.05). Conclusion: Taken together, these findings clearly showed that the toughness of the muscle highly depends and varies upon the temperature and ageing of the muscle. Moreover, the effect of cooking temperature was very limited on aged muscles. The results mirror the importance of cooking temperature for objective measurements which ultimately estimate sensory tenderness and other quality traits.

• Korean Journal of Applied Biomechanics
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• v.17 no.4
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• pp.209-219
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• 2007

The purpose of this study was to analyze biomechanical mechanism (posture, moment of back joint, EMG) when athletes (Judo, Ssirum) and lay people lifted a load according to two different lift methods; backlift and leglift. The number of subjects was 12; 8 athletes (4 for Judo, 4 for Ssirum) and 4 lay people. We recorded a lift motion in backlift and leglift using 7 real time infrared cameras (vicon) and analyzed EMG pattern of major muscles for a lift (lattisimus dorsi, erector spinae, biceps femoris). In a backlift Judo players showed a biggest range of back flexion and extension motion and lay people flexed more than other groups at phase 2 in which an interaction between groups and events was statistically significant (p=.024). In a leglift Ssirum players more flexed their back in a barbell lift and there was a statistical significance (p=.021) between groups and events. For moment of back joints, 1) in a backlift a larger loading on back joints in all three groups at phase 2 when lifting down a barbell, 2) in a leglift a larger loading on back joints when lifting down a barbell in two athlete groups but a larger loading when lifting up a barbell in lay people group, and all groups did not show any statistical significance. For EMG, right lattisimus dorsi muscle in a backlift was statistical significant (p=.006) in an interaction between groups and phase but left lattisimus dorsi muscle was insignicant, and there was not any significance in a leglift. Generally atheletes (Judo and Ssirum) used more their muscles of lower extremity in lifting up and down and lay people did more their ones of upper extremity.

• Physical Therapy Korea
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• v.12 no.1
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• pp.111-119
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• 2005

The purpose of this study was to compare the differences of hip and thigh muscle activities between subjects with increased and decreased femoral anteversion during stair ascent. Twelve healthy female volunteers participated in this study. The subjects were divided into two groups (group 1 with increased anteversion of the hip, group 2 with decreased anteversion of the hip). This study analyzed differences in each mean peak gluteus maximus (GM), gluteus medius (GD) and tensor fascia lata (TLF) EMG amplitude: composite mean peak hip muscles (GM, GD, TFL) EMG amplitude ratios and in each mean peak vastus medialis oblique (VMO), vastus lateralis (VL), biceps femoris (HM) and semitendinosus (HL) EMG amplitude: composite thigh muscles (VMO, VL, HM, HL) EMG amplitude ratios among subjects with decreased or increased relative femoral anteversion. EMG ratios were compared in the stance and swing phase of stair ascent. Group 1 showed an increased standardized mean GM and GD EMG amplitude and decreased standardized mean TFL to composite mean hip muscles EMG amplitude ratios in stair ascent during both stance and swing phase. Also, group 1 showed an increased standardized mean HL EMG amplitude and decreased standardized mean VL and HM to composite mean thigh muscles EMG amplitude ratios in stair ascent during both stance and swing phases. There was no statistically significant difference in vastus medialis oblique between subjects with increased or decreased relative femoral anteversion. In order to provide rehabilitation professionals with a clearer picture of the specific requirements of the stair climbing task, further research must be expanded to include a wider range of age groups that represent the general public, such as including middle-aged healthy persons.

• Korean Journal of Applied Biomechanics
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• v.20 no.2
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• pp.221-230
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• 2010

The purpose of this study was to analyze biomechanical factors of trail running shoes applied to korean shoe-lasts. 10 healthy male subjects with an average age of 37.2 years(SD=8.28), weight of 69.6 kg(SD=10.56) and a height of 171 cm(SD=4.93) were recruited for this study. Ten males walked on a treadmill wearing four different shoes. Foot pressure data was collected using a Pedar-X mobile system(Novel Gmbh., Germany) operating at the 1000 Hz. Surface EMG signals for tibialis anterior, gastrocnemius, vastus lateralis and biceps femoris were acquired at 1000 Hz using Noraxon TeleMyo DTS system(Noraxon Inc., USA). Foot pressure and leg muscle fatigue were measured and calculated during walking. The results are as follows: After walking 60 minutes, Type A showed a lower MPF. MPF values were significantly different from each muscle(p<.05). Therefore, Type A shoe might decrease muscle fatigue in the legs while walking. In addition, Type It showed that Type A shoe has the highest contact area and the lowest maximum pressure. As a result of the analysis, Trail running shoes will use a new design to reduce muscle fatigue and are expected to increase comfort and fitting.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.17 no.2
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• pp.25-32
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• 2011

Background: This study was to examine the effect of applying leg exercise using visual informations on improving stroke patient's muscle power, balance and gait. Methods: The subjects of this study were hemiplegic patients by stroke, 30 patients were picked up, who were agreed with this research and were having hospital care for 8 weeks at RS, RB and HH medical centers. The study group was 15 and comparison group was 15, totally 30 patients were confirmed. leg exercise using visual informations was applied to study group, leg exercise without visual informations was applied to comparison group. These were proceeded for 8 weeks (5 days a week, 20 minutes a day), change of muscle power, balance and gait was checked to investigate the effect. Electromyography apparatus was used for checking muscle power, K-BBS (Korean version of Berg Balance Scale) and COP (center of pressure) were used for balance, then Functional Gait Assessment (FGA), timed up and go test (TUG) were used for checking the capacity of gait. Results: Vastus lateralis (p=.002), biceps femoris (p=.000), K-BBS (p=.000), COP (p=.007), FGA (p=.002), TUG (p=.009) are significant difference between the two groups. Conclusion: This study showed that visual informations impact change of muscle activity, balance and locomotor ability in stroke patients.

• Journal of the Ergonomics Society of Korea
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• v.28 no.1
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• pp.9-19
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• 2009

Repetitive movement, inadequate working posture, overuse of force, physical contact with sharp edge, vibration and temperature were well known risk factors of WMSDs (Work-related Musculoskeletal Disorders). Many researchers have investigated the relationship between inadequate working postures and incidences of WMSDs of the upper extremities, whereas relatively few researchers have tried to evaluate workload associated with the lower-body postures. The effects of lower-limb postures including various knee flexion angles on the subjective discomfort, heart rate and EMG of lower-limb muscles were investigated in this study. Thirty graduate students were asked to maintain thirteen different body postures, and heart rate and EMG data of five muscle groups (electro spine, biceps femoris, vastus medialis, gastrocnemius and tibialis anterior) from each posture were collected during fifteen minutes sustaining tasks. All participants were also asked to report their discomfort ratings of body parts. Results showed that high subjective discomfort ratings and heart rates were reported at the postures of knee angles of $60^{\circ}$ and $90^{\circ}$, whereas low discomfort ratings were founded at the postures of chair heights with 20cm, 40cm, and sitting with crossed legged. The change of median frequency for each muscle group during fifteen minutes tasks was investigated for each body posture to evaluate the relationship between muscle fatigue and body posture. It was found that the trends of changes of median frequency were different based on muscle group as well as lower-limb body posture from this study.

• Physical Therapy Rehabilitation Science
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• v.6 no.1
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• pp.39-44
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• 2017

Objective: The purpose of this study is to show the effectiveness of performing squat exercises at various angles to show the maximum muscle activity of the Vastus Medialis Oblique (VMO) and Biceps femoris (BF). Design: Cross-sectional study. Methods: A total of seventeen healthy young adults (8 males and 9 females) voluntarily participated in the study. All subjects randomly performed three different squat variations as follows: A squat performed with the ankle joint at $0^{\circ}$ of incline, a squat performed with the ankle joint at $5^{\circ}$ of incline, and a squat performed with the ankle joint at $10^{\circ}$ incline. Muscle activity was measured using surface electromyography. Electrodes were placed on the VMO and BF to measure the muscle activity on the various ankle angles for comparison analysis. Results: There was a significant increase in bilateral VMO muscle activation at $10^{\circ}$ of incline compared to $0^{\circ}$ and $5^{\circ}$ (p<0.05). Greater increases in muscle activation and exercise effect was observed with increasing incline angles of the board. Changes in bilateral BF muscle activity were found; however, none were found to be significant. Conclusions: Bilateral VMO activity was found to be significant when the squats were performed at an ankle angle of $10^{\circ}$ of incline when compared to at an ankle angle of $0^{\circ}$ and $5^{\circ}$ of incline. Squats performed on an incline can be recommended as an effective method to facilitate lower extremity muscle activities.

• Physical Therapy Rehabilitation Science
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• v.6 no.1
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• pp.14-19
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• 2017

Objective: To investigate the effect of performing three different bridge exercise conditions on the activities of four different muscles using surface electromyography (sEMG) in healthy young adults. Design: Cross-sectional study. Methods: A total of 20 healthy young adults (10 males, 10 females) voluntarily participated in this study. All subjects randomly performed three different bridge conditions as follows: general bridge exercise, isometric hip abduction (IHAB) with a blue Theraband (Hygenic Corp., USA), and isometric hip adduction (IHAD) with a Swiss ball (Hygenic Corp.). The muscle activities of bilateral erector spinae (ES), gluteus maximus (GM), biceps femoris (BF), and external oblique (EO) muscles during the bridge exercises were measured using sEMG. Subjects performed each of the three bridge conditions three times in random order and mean values were obtained. Results: For bilateral ES and BF, there was a significant increase in muscle activity in the IHAD condition compared to the general bridge and IHAB condition (p<0.05). For bilateral GM, there was a significant increase in muscle activity in the IHAB condition compared to the general bridge condition (p<0.05) and there was a significant increase in muscle activity in the IHAB condition compared to IHAD condition (p<0.05). For left EO, a significant increase was observed in the IHAD condition compared to the general bridge condition (p<0.05). Conclusions: ES and BF muscle activity increases were observed with hip adduction and increased GM activity was observed with hip abduction. These findings may be applicable within the clinical field for selective trunk and lower extremity muscle activation and advanced rehabilitation purposes.