• Title/Summary/Keyword: Bi-stability

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전기화학적 방법을 이용한 다중 효소 전극 제작 및 글루코스 검출 특성에 관한 연구 (A Study on the Detection Characteristics in Glucose and Fabrication of Bi-Enzyme Electrode using Electrochemical Method)

  • 한경호;신인성;윤도영
    • 전기화학회지
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    • 제23권3호
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    • pp.66-72
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    • 2020
  • 본 연구에서는 글루코스 검출을 위한 HRP (horseradish peroxidase)와 GOD (glucose oxidase)를 혼합한 형태의 다중 효소 반응 바이오센서 개발연구를 수행하였다. 바이오센서는 제작 시간 단축을 위하여 전기전착법을 이용하여 제작하였으며, 경제적인 센서 제작을 위하여 SPE (screen printed electrode)를 사용하였다. 다중 효소 바이오센서의 효과를 확인하기 위하여 단일 효소 바이오센서를 제작하여 비교 및 분석하였다. 센서의 특성을 평가하기 위해서 주사전자현미경(scanning electron microscopy, SEM), 순환전위법(cyclic voltammetry, CV), 전기화학적 임피던스 분광법(electrochemical impedance spectroscopy, EIS), 시간대전류법(chronoamperometry, CA), 흐름 주입분석법(flow injection analysis, FIA)를 수행하였다. SEM, CV, EIS의 분석결과로부터 효소가 전극 표면에 고정화가 잘 된 것을 확인하였고, CA로부터 제작된 다중 효소 바이오센서가 단일 효소 바이오센서에 비해 신호성능이 200% 향상된 것을 확인하였다. 이로부터 HRP와 GOD가 서로 촉매적으로 반응한다는 것을 설명할 수 있었다. 또한, FIA의 결과에서 동일한 농도의 글루코스 용액을 4회 나누어 주입하였을 때, 전류신호량이 일정함을 확인하였고, 농도에 따른 전류신호량을 분석하여 신호민감성, 재현성, 안정성 등이 우수함을 설명할 수 있었다.

Cellular Biomarker of Membrane Stability and Hydrolytic Enzyme Activity in the Hemocytes of Benzo(a)pyrene-exposed Pacific oyster, Crassostrea gigas

  • Jo Qtae;Choy Eun-Jung;Park Doo Won;Jee Young-Ju;Kim Sung Yeon;Kim Yoon
    • Fisheries and Aquatic Sciences
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    • 제5권4호
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    • pp.263-270
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    • 2002
  • The Pacific oysters, Crassostrea gigas, were stressed with different concentrations of benzo(a) pyrene and depurated to determine the hemocyte lysosomal membrane stability and hydrolytic enzymatic activity as a biomarker candidate to the chemical, using NRR (neutral red retention) and API ZYM System, respectively. The membrane damage measured as NRR decrease was significant with the increase of chemical concentration and exposure time (P<0.05), providing a possible tool for biomarker. Interestingly, the control showed intrinsic stress probably due to captive life in the laboratory, and a recovering trend was also found during the depuration. The benzo(a)pyrene-exposed oysters showed increased enzyme activities in alkaline phosphatase, esterase (C4), acid phosphatase, naphthol-AS-BI-phospho­hydrolase, $\beta$-galactosidase, $\beta$-glucuronidase, and N-acetyl- $\beta$-glucosaminidase. Of them, only two enzymes, acid phosphatase and alkaline phosphatase, showed some potential available for the generation of enzymatic biomarker in the oyster. The results are suggestive of the potential availability of the cellular and enzymatic properties as a biomarker. However, considering that a robust biomarker should be insensitive to natural stress coming from normal physiological variation, but sensitive to pollutants, a concept of intrinsic stress the animal possesses should be taken into consideration. This reflects the necessity of further research on the intrinsic stress affecting the cellular and enzymatic properties of the chemical­stressed oysters prior to using the data as a biomarker.

Effect of Low-Temperature Sintering on Electrical Properties and Aging Behavior of ZVMNBCD Varistor Ceramics

  • Nahm, Choon-Woo
    • 한국재료학회지
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    • 제30권10호
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    • pp.502-508
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    • 2020
  • This paper focuses on the electrical properties and stability against DC accelerated aging stress of ZnO-V2O5-MnO2-Nb2O5-Bi2O3-Co3O4-Dy2O3 (ZVMNBCD) varistor ceramics sintered at 850 - 925 ℃. With the increase of sintering temperature, the average grain size increases from 4.4 to 11.8 mm, and the density of the sintered pellets decreases from 5.53 to 5.40 g/㎤ due to the volatility of V2O5, which has a low melting point. The breakdown field abruptly decreases from 8016 to 1,715 V/cm with the increase of the sintering temperature. The maximum non-ohmic coefficient (59) is obtained when the sample is sintered at 875 ℃. The samples sintered at below 900 ℃ exhibit a relatively low leakage current, less than 60 mA/㎠. The apparent dielectric constant increases due to the increase of the average grain size with the increase of the sintering temperature. The change tendency of dissipation factor at 1 kHz according to the sintering temperature coincides with the tendency of the leakage current. In terms of stability, the samples sintered at 900 ℃ exhibit both high non-ohmic coefficient (45) and excellent stability, 0.8% in 𝚫EB/EB and -0.7 % in 𝚫α/α after application of DC accelerated aging stress (0.85 EB/85 ℃/24 h).

추력 30톤급 액체산소/케로신 로켓엔진 연소장치 개발(I)-연소기 (Development of 30-Tonf LOx/Kerosene Rocket Engine Combustion Devices(I) - Combustion Chamber)

  • 최환석;한영민;김영목;조광래
    • 한국항공우주학회지
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    • 제37권10호
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    • pp.1027-1037
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    • 2009
  • 우주발사체용 30톤급 액체로켓엔진 재생냉각 연소기 개발과정에 관하여 기술하였다. 이중추진제 동축 와류형 분사기 개발에서부터 시작하여 축소형 연소기를 통해 요소기술을 검증하고 개발된 기술을 실물형 연소기에 적용하였다. 총 5기의 실물형 연소기 개발시제를 사용하여 점화성능, 연소안정성, 연소성능, 냉각성능, 내구성 등의 검증을 수행하였다. 이 과정에서 총 46회의 실물형 연소기 연소시험을 실시하였고 이 중 23회는 기폭장치를 이용한 연소안정성 평가시험을 병행하였다. 시험 결과 30톤급 재생냉각 연소기는 연소 성능 및 연소안정성 요구사항을 모두 만족시켜 단품 개발이 성공적으로 완료된 것으로 평가하였다.

Parametric Instability Boundaries for Spatial Subharmonics in Photorefractive Moving Gratings: Theory and Experiments

  • Kwak, Chong-Hoon;Lee, El-Hang
    • ETRI Journal
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    • 제16권4호
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    • pp.13-25
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    • 1995
  • We have derived a nonlinear spatiotemporal differential equation for space-charge fields from Kukhtarev’s material equations in a moving coordinate system and obtained the spatial subharmonic instability boundaries by using linear stability analysis. It is also found that there is an analogy between the temporal subharmonic and the spatial subharmonc instabilities in the sense that the governing differential equations describing the instability boundaries are formally identical. The experiments for generating spatial subharmonic waves are performed in a photorefractive $Bi_{12}SiO_{20}$ crystal by using conventional moving grating technique. The threshold detunings are experimentally determined and the results are compared with the theory.

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Experimental Realization of Matrix Converter Based Induction Motor Drive under Various Abnormal Voltage Conditions

  • Kumar, Vinod;Bansal, Ramesh Chand;Joshi, Raghuveer Raj
    • International Journal of Control, Automation, and Systems
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    • 제6권5호
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    • pp.670-676
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    • 2008
  • While the matrix converter has many advantages that include bi-directional power flow, a size reduction, a long lifetime, and sinusoidal input currents, it is vulnerable to the input voltage disturbances, because it directly exchanges the input voltage to the output voltage. So, in this paper, a critical evaluation of the effect of various abnormal voltage conditions like unbalanced power supply, balanced non-sinusoidal power supply, input voltage sags and short time blackout of power supply on matrix converter fed induction motor drives is presented. The operation under various abnormal conditions has been analyzed. For this, a 230V, 250VA three phase to three phase matrix converter (MC) fed induction motor drive prototype is implemented using DSP based controller and tests have been carried out to evaluate and improve the stability of system under typical abnormal conditions. Digital storage oscilloscope & power quality analyzer are used for experimental observations.

Design of a Wideband Antipodal Vivaldi Antenna with an Asymmetric Parasitic Patch

  • Bang, Jihoon;Lee, Juneseok;Choi, Jaehoon
    • Journal of electromagnetic engineering and science
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    • 제18권1호
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    • pp.29-34
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    • 2018
  • An antipodal Vivaldi antenna with a compact parasitic patch to overcome radiation performance degradations in the high-frequency band is proposed. For this purpose, a double asymmetric trapezoidal parasitic patch is designed and added to the aperture of an antipodal Vivaldi antenna. The patch is designed to efficiently focus the beam toward the end-fire direction at high frequencies by utilizing field coupling between the main radiating patch and the inserted parasitic patch. As a result, this technique considerably improves the gain and stability of radiation patterns at high frequencies. The proposed antenna has a peak gain greater than 9 dBi over the frequency range of 6-26.5 GHz.

Bi계 은시스산화물초전도테프의 쿠엔치전파특성의 온도의존성 (Temperature Dependance of Propagation Velocity in a Silver Sheathed Bi-family Superconducting Oxid Tape)

  • 김석범;아오끼게스케;이시야마아쯔시
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1994년도 하계학술대회 논문집 A
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    • pp.76-78
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    • 1994
  • Owing to the discover of oxide superconducting over critical temperature100K, it is being made experimentally somewhere under the possibility that there is superconducting magnet by cooling liquid nitrogen. The issues of thermal stability and quench process of Low-Tc superconductor has been studied and used application of oxide superconducting magnets. However the quench propagation property of oxide superconductor, especially experimental data about thermal behavior has not been reported yet. Therefore we measured the effect of temperature dependance of quench propagation velocity, Vq, by using the short samples made up of silver sheathed Bismuth-family (2223phase) superconducting oxide tape.

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An Efficient Monitoring Method of a Network Protocol for Downloadable CAS

  • 정영호;권오형;안충현;홍진우
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송공학회 2010년도 하계학술대회
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    • pp.32-35
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    • 2010
  • This paper presents an efficient monitoring method of a network protocol for a downloadable conditional access system (DCAS) that can securely transmit conditional access software via a bi-directional communication channel. In order to guarantee a secure channel based on mutual authentication between a DCAS head end server and set-top boxes, DCAS messages are encrypted and digitally signed. Owing to applied cryptographic algorithms, it is impossible to get information from messages directly without additional processing. Through categorizing DCAS messages into several groups, the proposed monitoring method can efficiently parse and trace DCAS messages in real-time. In order to verify the stability and effectiveness of the proposed monitoring method, we implement a DCAS monitoring system capable of capturing and parsing all DCAS messages. The experimental results show that the proposed monitoring method is well designed.

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아동의 항상성개념 발달에 관한 연구 - 자신 및 타인의 개인특성을 중심으로 - (Development of the Character Constancy Concept: Self and Others)

  • 김창하
    • 아동학회지
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    • 제8권1호
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    • pp.15-29
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    • 1987
  • The purpose of the present research was to investigate (I) the acquisition of character constancy of self and others as a function of age; (2) the pattern of acquisition of character constancy, and (3) the relationships between character constancy and conservation of number and mass. The subjects of this study were 160 children, 20 boys and 20 girls at each grade level, kindergarter through grade 3. The Character Constancy Tasks devised bi Rotenberg (1982) and conservation task of number and mass were administered to each child. Chi square, Guttman Scale analysis and Pearson's Correlation were used for the statistical analysis of data. The findings showed that there was increase with age in character constancy of others and of sell in that there was increase with age in children's belief that the characteristics of another person are stable across time and consistent despite changes in a person's appearance. Character constancy of others and of self were acquired in the developmental stages of identity, stability, and consistency. Character constancy of others and self correlated with the conservation of number and mass.

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