• Korean Journal of Food Science and Technology
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• v.21 no.3
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• pp.446-452
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• 1989

The pigment of the Korean cockscomb flower, a betacyanin, was evaluated for its stability in terms of temperature, pH, and its behavior upon exposure to water, light, and air. The pigment was the most stable at pH 4.0, and its activation energy (Ea) for degradation was shown to be 17.55Kcal/mol. In general, sugars protected against color degradation at the concentration of 0.1M. Degradation of this pigment in the presence of food constituents, such as organic acids , metal ions, or antioxidants, at the concentrations normally present in food preparations, can be kept to a minimum by selective adjustment of conditions. This pigment, therefore, has potential value as a food colorant under selected conditions.

• Proceedings of the Korea Contents Association Conference
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• 2013.05a
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• pp.361-362
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• 2013

As one of the steps toward understanding how the plant is well adapted to strongly saline habitats, the purple pigment compound that is accumulated in Suaeda japonica was extracted and characterized. The extracted pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. LC-MS of the extracted pigment compound showed the presence of two major protonated molecular ions ($[M+H]^+$) at m/z 651.1 and m/z 827.1. According to the DPPH assay, it was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 mg/ml. The cytotoxic activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.

• Journal of Food Hygiene and Safety
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• v.36 no.5
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• pp.376-381
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• 2021

Red beet (Beta vulgaris L.) is a root vegetable and a popular functional food ingredient of dark red-purple appearance due largely to betacyanins, principally betanine (75-95%) and its isomer, isobetanine (15-45%). This study developed an analytical method for beet red in terms of betanine and isobetanine in processed food products labeled with beet red as a food additive. High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) was used with a C₁₈ column. Linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision and uncertainty in measurement were calculated for method validation. Matrix-matched calibration was applied to the candy, ice cream, and cocoa product, respectively, and R² was ≥0.9998, showing a high level of linearity. The LOD and LOQ were 0.16 to 0.32 and 0.48 to 0.97 mg/L, respectively. As a result of repeated intra-day and interday experiments to validate the accuracy and precision of the analytical method, the recovery rates were 96.0-103.1% and 100.0-102.2%, respectively and the RSD% was 0.5-3.3% and 0.9-3.8%, respectively. Moreover, the measurement uncertainty was estimated to be 1.71-12.43% depending on the matrix and the measured concentration. In this study, betanine and isobetanine were quantified (8.4-3,823.4 mg/kg) by applying the developed analytical method to processed food products (n= 26; e.g., candy, ice cream, and other processed foods) labeled with beet red as a food additive.

• Korean Journal of Environmental Agriculture
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• v.37 no.3
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• pp.197-206
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• 2018

BACKGROUND: The present study aimed to examine the crops capable of growing and adapting to the external environment and various stresses of reclaimed agriculture land for the development of high value-added agricultural utilization technology based on reclaimed land through standardization and empirical study of cultivation environment for cultivating crops. METHODS AND RESULTS: Two crops namely turnips and beets were selected for the salt tolerance test of soil environmental conditions on reclaimed land. Turnip and beet seedlings were planted on the soil collected at the 'Seokmun' reclaimed land. There are five treatments such as non-treatment, 1.0, 2.0 (control), 4.0 and $8.0dS{\cdot}m^{-1}$ of EC. The contents of betacyanin in beet roots was highest in control and decreased with increasing salt concentration. The GSL contents in the turnip roots waswere highest at EC 2.0 and decreased with increasing salt concentration, whereas those in turnip leaves waswere high both in the non-treated control and atthe EC 1.0-treatment. But, tThere was, however, no statistical differences among the treatments. CONCLUSION: The degree of salt tolerance of crops was examined, and the limit EC iswas expected to be $3.0{\sim}4.0dS{\cdot}m^{-1}$ as reported to date. If the soil improvement is performed and irrigation systems are used in the actual reclaimed land, the EC of supplied irrigation will be low, and desalination effecttreatment by the lower EC of the supplied irrigation on the soil will lead to more favorable soil condition of the rhizosphere and cultivation environment offor the crops than those in the port experiment. Therefore, monitoring the salinity, water content and ground water level will enable prediction of the rhizosphere environment, and setting up irrigation management and supplying irrigation will lead to crop cultivation results that are close to normal.