• Title/Summary/Keyword: Beta-mixture

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Phytochemical Studies on Paeoniae Radix (4);Cerebrosides and Other Constituents

  • Kim, Yoon-Jung;Yean, Min-Hye;Lee, Eun-Ju;Kim, Ju-Sun;Lee, Je-Hyun;Kang, Sam-Sik
    • Natural Product Sciences
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    • v.14 no.3
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    • pp.161-166
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    • 2008
  • A mixture of sixteen cerebrosides, which comprised four cerebroside molecular species (PL-1 ${\sim}$ PL-4) was separated from the roots of Paeonia lactiflora. The structures of cerebrosides were characterized as $1-O-{\beta}$-D-glucopyranosides of phytosphingosines, which comprised a common long-chain base, (2S,3S,4R,8E/Z)-2-amino-8-octadecene-1,3,4-triol with eight fatty acids or 2-hydroxy fatty acids of varying chain lengths ($C_{16}$, $C_{18}$, $C_{20-26}$) linked to the amino group. Aralia cerebroside and its 8Z isomer (PL-1), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S, 4R,8E/Z)-2-[(2'R)-2'-hydroxytetracosanoylamino]-8-octadecene-1,3,4-triol (PL-2), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxydocosanoylamino]-8-octadecene-1,3,4-triol (PL-3), and $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxytricosanoylamino]-8-octadecene-1,3,4-triol (PL-4) were identified as major components of these cerebroside molecular species. All the major cerebrosides were shown to be a mixture of geometrical isomers (8E and 8Z) of phytosphingosine-type glucocerebrosides possessing 2R-hydroxy fatty acids. In addition, three ${\beta}-sitosterol$ derivatives and adenosine were also separated. The structures of these isolates have been determined on the basis of chemical and spectroscopic evidence.

A Study for the Physical Properties of Artificial Admixtured with β-NSF Base & Vinsol Base Surfactants (β-NSF계와 빈졸계 계면활성제로 변성된 인조석의 물성)

  • Cho, Heon-young;Park, Seong-ki;Suh, Jung-mok;Kim, Jin-man
    • Applied Chemistry for Engineering
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    • v.10 no.4
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    • pp.592-598
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    • 1999
  • Exterior finishing materials of artificial stones are manufactured with the mixture of water, cement, stone powder and light-weight aggregate. In this research, we tried to find a way of increasing the physical properties and decreasing the manufacturing cost of artificial stone. So, we used ${\beta}$-NSF base surfactant and vinsol base surfactant to the artificial stone mixture instead of light-weight aggregate. The optimum dosage of the ${\beta}$-NSF and vinsol surfactants for artificial stone are found to be 1.0 wt % of cement, respectively. The physical properties increased ca. 20% and the durability for freezing & thawing of the new artificial stone increased ca. 300%. While the manufacturing cost of the new artificial stone decreased as much as 30%.

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Radioassay of Multiple Beta-Labeled Mixtures using Least-Square Method (최소자승법을 이용한 다수 베타 방출 핵종 혼합물의 방사능 분석)

  • Seon, Kwang-Il;Nam, Uk-Won;Kong, Kyoung-Nam;Kim, Chang-Kyu;Lee, Dong-Myung;Lee, Sang-Kook
    • Journal of Radiation Protection and Research
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    • v.26 no.4
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    • pp.375-384
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    • 2001
  • It is possible to count and perform quench correction on two ${\beta}$ -label samples so long as the maximum ${\beta}$-energies are sufficiently different. However, when 4he conventional technique is applied to the radioassay of a mixture of more than three nuclides, the reliability of the activities determined is considerably reduced, resulting from the large overlapping of liquid scintillation pulse height distributions of each nuclide. A technique that allows the activities of multiple ${\beta}$-labeled samples to be radioassayed was proposed by using the least square method. The technique was applied to mixture samples of $^3H,\;^{14}C,\;^{36}Cl$, and $^{90}Sr$. The analytical values were in good agreement with the reference values within 7% relative error.

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Studies on the Constituents of the Roots of Rubus parvifolius (I) -Isolation of (-)-Epicatechin- (멍석딸기뿌리의 성분(成分)에 관한 연구(硏究)( I ) -(-)-Epicatechin의 분리(分離)-)

  • Do, Jae-Chul;Son, Kun-Ho;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.19 no.3
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    • pp.170-173
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    • 1988
  • (-)-Epicatechin, $mp\;234{\sim}6^{\circ}$, was isolated from the fresh roots of Rubus parvifolius together with a sterol mixture, which was found to be a mixture of ${\beta}-sitosterol\;(major)$, stigmasterol and campesterol.

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Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip

  • Park, Nam-Yong;Na, Sung-Ho;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.30 no.4
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    • pp.505-510
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    • 2007
  • This study was conducted to detect ${\beta}$-lactam antibiotic-resistant genes in the 400 E coli isolates from porcine fecal samples in Korea by a DNA chip. The DNA chip contains the specific probe DNAs of the ${\beta}$-lactam antibiotic-resistant genes that had been labeled with a mixture of primer set designed to amplify specific genes (PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY and AmpC) using a multiplex polymerase chain reaction (PCR). Of 400 isolates 339 contained at least one ${\beta}$-lactamases gene. Resistance to ${\beta}$-lactamases was mediated mainly by AmpC (n = 339, 100%), and followed by TEM (n = 200, 59.0%), CMY (n = 101, 29.8%), PSE (n = 30, 8.9%) and both OXA and SHV genes (n = 20, 5.9%), while the FOX, MEN and OXY genes were not detected. The other sixty-one did not contain any ${\beta}$-lactamase genes even though they were resistant to antimicrobial drugs. In conclusion, the DNA chip system can be used as a rapid and reliable method for detecting of ${\beta}$-lactamases genes, which will help veterinarians select the antibiotics for monitoring and treating of animal diseases.

A Study on Synthesis of High Purity $\beta$-SiC Fine Particle from Ethylsilicate(I) -Reaction Conditon, Yeild and Properties of $\beta$-SiC- (Ethyl Silicate를 고순도 $\beta$-SiC미분말 합성에 관한 연구(I) 반응조건과 $\beta$-SiC의 생성율 및 특성)

  • 최용식;박금철
    • Journal of the Korean Ceramic Society
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    • v.25 no.5
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    • pp.473-478
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    • 1988
  • In order to obtain the high purity $\beta$-SiC powder that possesses the excellent sinterability and is close to the spherical shape, the carbon black was mixed into the composition of Si(OC2H5)4-H2O-NH3-C2H5OH which the monodispersed spherical fine particles is formed the hydrolysis of Ethylsilicate and the mixture was carbonized under an argon atmosphere. Particle shpae, size and the yield of $\beta$-SiC powder were investigated according to the molar ratio of carbon/alkoxide and variations of reaction temperature and reaction time. The results of this study are as follow ; 1) The yield of $\beta$-SiC gained from the reaction for one hour at 150$0^{\circ}C$ almost got near 100% and the particle size of $\beta$-SiC from the reaction for 15 hrs at 150$0^{\circ}C$ was 0.2${\mu}{\textrm}{m}$ on the average and close to the spherical shape agglomerate state. 2) When the molar ratio carbon/alkoxide is over 3.1 and the reaction occurs at 145$0^{\circ}C$ for 5hrs, the carbon content has not an effect on the kind of crystal of product.

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Fabrication of Blended PCL/β-TCP Scaffolds by Mixture Ratio of β-TCP using Polymer Deposition System (폴리머 적층 시스템을 이용한 β-TCP 혼합 비율에 따른 PCL/β-TCP 인공지지체의 제작)

  • Ha, Seong-Woo;Kim, Jong Young
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.9
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    • pp.791-797
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    • 2014
  • Abstract Scaffold used as a carrier of the cell has been actively conducted using plenty of technology in tissue engineering. ${\beta}$-tricalcium phosphate (${\beta}$-TCP) material has shown good biocompatibility and osteoconductive ability when it was implanted as a bone graft substitute in osseous defect in human and animal studies for bone regeneration. In this study, we fabricated the blended polycaprolactone (PCL) and ${\beta}$-TCP scaffold by the polymer deposition system (PDS). The PCL/${\beta}$-TCP scaffold was fabricated at a temperature of $110^{\circ}C$, pressure of 650 kPa, and scan velocity of 100 mm/sec. The Overall geometry and size of the scaffold were fixed circle type with a diameter of 10 mm and a height of 4 mm. PCL/${\beta}$-TCP scaffold was observed by scanning electron microscopy. Cell attachment and proliferation of the scaffold containing 30 wt% ${\beta}$-TCP was superior to those containing 10 wt% and 20 wt% ${\beta}$-TCP.

Detection of $\beta$-lactam Antibiotic-resistant Genes in Eschericia coli using DNA Chip from Porcine Fecal Samples

  • Na, Sung-ho;Cho, Ho-seong;Kim, Yong-hwan;A.W.E. Effendy;Park, Nam-yong
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2003.10a
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    • pp.33-33
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    • 2003
  • There prevalence of $\beta$-lactamases bacteria in animals has been increased since 1990s [1]. The resistance in E coli which is mediated by $\beta$-lactamases hydrolyze the $\beta$-lactam ring eventually inactivate the antibiotics [2]. Generally, $\beta$-lactamases can be classified into four main groups and eight subgroups according to their functional and structural characteristics [3]. The detection of $\beta$-lactam antibiotic-resistant bacteria by DNA chip has been described [4]. The chip has a specific probe DNAs that contained the $\beta$-lactam antibiotic-resistant genes which was labeled by multiplex PCR reaction with a mixture of primer sets that were designed to amplify specific gene. Here we report the susceptibility of enteropathogenic E. coli isolated from pigs in Korea using the DNA chip in detecting $\beta$-lactam antibiotic-resistant genes. (omitted)

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Electrospinning of Asiaticoside/2-Hydroxypropyl-β-cyclodextrin Inclusion Complex-loaded Cellulose Acetate Fiber Mats: Release Characteristics and Potential for Use as Wound Dressing (Asiaticoside/2-Hydroxypropyl-β-cyclodextrin 포접화합물 함유 셀룰로오스 아세테이트 섬유 매트의 전기방사: 창상피복제로서 사용가능성과 방출특성)

  • Panichpakdee, Jate;Pavasant, Prasit;Supaphol, Pitt
    • Polymer(Korea)
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    • v.38 no.3
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    • pp.338-350
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    • 2014
  • Cellulose acetate (CA) fiber mats containing inclusion complexes of asiaticoside (AC) in 2-hydroxypropyl-${\beta}$-cyclodextrin ($HP{\beta}CD$) for potential usage as wound dressings were developed. The AC/$HP{\beta}CD$ complex-loaded CA fibers at various $HP{\beta}CD$ to AC molar ratios of 0.5, 1, and 2 were prepared in 90:10 v/v mixture of 80% (v/v) acetic acid and N,N-dimethylacetamide (DMAc) via electrospinning. The maximum released amounts of AC depended on the $HP{\beta}CD$ content and were much greater than those released from the AC-loaded CA fiber mat. In the in vitro study, indirect cytotoxic evaluation with human dermal fibroblasts (HDFa) showed that these materials released no substances in the levels that were harmful to the cells and the cells appeared to attach and proliferate well on these substrates. However, only the CA fiber mats containing AC/$HP{\beta}CD$ complexes at the $HP{\beta}CD$ to AC molar ratio of 0.5 was effective in upregulating the production of collagen of the cultured cells.

Preparation of β-SiAlON Powder by Combustion Reaction in the System of Si-Al-SiO2-NH4F(β-Si3N4) (Si-Al-SiO2-NH4F(β-Si3N4)계에서 연소반응에 의한 β-SiAlON분말의 제조)

  • Min, Hyun-Hong;Shin, Chang-Yun;Won, Chang-Whan
    • Journal of the Korean Ceramic Society
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    • v.43 no.10 s.293
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    • pp.595-600
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    • 2006
  • The preparation of $\beta$-SiAlON powder by SHS in the system of $Si-Al-SiO_2-NH_4F(\beta-Si_3N_4)$ was investigated in this study. In the preparation of SiAlON powder, the effect of gas pressure, compositions such as Si, $NH_4F$, \beta-Si_3N_4$ and additive in mixture on the reactivity were investigated. At 50 atm of the initial inert gas pressure in reactor, the optimum composition for the preparation of pure $\beta$-SiAlON was $3Si+Al+2SiO_2+NH_4F$. The $\beta$-SiAlON powder synthesized in this condition was a single phase $\beta$-SiAlON with a rod like morphology.