• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.93-97
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• 2006

Objective : This study was to evaluate the effectiveness of Bee Venom acupuncture therapy on external epicondylitis. Methods : We divided chronic arthritis of ankle patient into 2 groups; one group combined bee venom acupuncture therapy and acupuncture therapy, another group was only acupuncture therapy. To estimate the effectiveness of treatment that applied for two groups, we used visual analog scale(VAS). We compared the VAS score of two groups statistically. Results : 1. As a result of evaluation by using visual analog scale(VAS), treatment score at final was marked more higher than score before treatment on each groups. 2. treatment at final, acupuncture and bee venom acupuncture therapy group had significant result on visual analog scale(VAS) compared with acupuncture therapy group. Conclusion : Bee Venom acupuncture therapy can be used with acupuncture therapy for highly effective treatment for external epicondylitis.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.167-171
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• 2006

Objective : This study was to evaluate the effect of Bee venom-Acupuncture therapy on patients with sprain of the wrist. Methods : We investigated 31 cases of patients with sprain of the wrist. We flip a coin and divide patients into two groups. Bee venom-Acupuncture was performed at on group, and the other group we didn't do it. We evaluated the treatment effect of each group by using the visual analog scale(VAS). Results : 1. As a result of evaluation by using the VAS, the score after treatment was marked lower than that before treatment within each group. 2. After treatment, Bee venom-Acupuncture therapy group showed significant difference on visual analog scale(VAS) compared with acupuncture therapy group. Conclusion : These results suggested that Bee venom-Acupuncture treatment should be more effective in the patient with sprain of the Wrist joint.

• Journal of Pharmacopuncture
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• v.7 no.1 s.12
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• pp.71-75
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• 2004

Objectives : This study was conducted to establish a standard and classify suitability in the treatment of limb impediment among the children with development disabilities using bee venom and eight principles herbal acupuncture. Methods : 10 patients with pediatric development disabilities with limb impediment as the main symptoms were chosen in this study. Bee venom herbal acupuncture and eight principles herbal acupuncture treatments were rendered and evaluated responses as well as pursuing most proper treatment methods. Results : 1. Bee venom herbal acupuncture showed a significant effects when used as supplement treatment technique for the children with partial movement, but insignificant for the children whom were unable to move. 2. Bee venom was effective for severe limb impediment and limb asthenia, whileas eight principles herbal acupuncture effective for mild limb impediment and spasticity. 3. Bee venom herbal acupuncture used in conjunction with eight principles herbal acupuncture for treating limb impediment among the children with development disabilities showed general improvement by intensifying muscular strength.

• Journal of Pharmacopuncture
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• v.10 no.2 s.23
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• pp.93-98
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• 2007

Objectives : The aim of this study was to observe variation of Bee Venom content from the collection period. Methods : Content analysis of Bee Venom was rendered using HPLC method by standard melittin Results : Analyzing melittin content using HPLC, 478.97mg/g at june , 493.89mg/g at july, 468.18mg/g at August and 482.15mg/g was containing in Bee Venom at september. So the change of melittin contents was no significance from June to September. Conclusion : Above these results, we concluded carefully that collecting time was not important factor for the quality control of Bee Venom, restricted the period from June to September.

• Journal of Acupuncture Research
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• v.31 no.2
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• pp.1-10
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• 2014

Objectives : The aim of this study was to analyze the interrelation of therapeutic effects according to the bee venom pharmacopuncture-induced skin hypersensitivity reaction and the sasang constitution. Methods : Data were collected from 62 patients who had been treated with bee venom pharmacopuncture due to low back pain. The patient's symptoms were evaluated with visual analogue scale(VAS), oswestry disability index(ODI). And we classified the patients according to the manifestation of skin hypersensitivity reaction, sasang constitution by QSCC II. Results : 22 patients had the skin hypersensitivity reaction, 40 patients hadn't. And 12 patients were Soeumin, 30 patients were Soyangin, 17 patients were Taeeumin. After treatment with bee venom pharmacopuncture 6 times(three times per week for 2 weeks), VAS was changed from $6.15{\pm}1.63$ to $4.00{\pm}1.64$. ODI was changed from $19.61{\pm}8.15$ to $14.76{\pm}8.07$. And there were significant associations. By the change of VAS and ODI between skin hypersensitivity reaction group and not, there were no significant associations. By the occurrence of skin hypersensitivity reaction between the sasang constitution, there were no significant associations. By the change of VAS and ODI between the Sasang constitution, there were no significant associations. Conclusion : This study shows that there were no significant associations in the frequency of skin hypersensitivity reaction according to the Sasang constitution. And the therapeutic effects of bee venom pharmacopuncture had no concern with skin hypersensitivity reaction and the Sasang constitution.

• Journal of Acupuncture Research
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• v.33 no.4
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• pp.165-180
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• 2016

Objectives : This study is aimed to report the effectiveness of ultrasound-guided bee venom pharmacopuncture in rotator cuff disease patients. Methods : We treated 4 rotator cuff disease patients with ultrasound-guided bee venom pharmacopuncture and integrative Korean Medical Treatment. The Numeric Rating Scale(NRS), Shoulder Pain And Disability Index(SPADI), and Range Of Motion(ROM) were applied as outcome measures. Results : Comparing before and after treatment, both NRS and SPADI scores were decreased, and ROM was increased in all cases. And adverse events about bee venom pharmacopuncture have not been observed. Conclusion : This study shows ultrasound-guided bee venom pharmacopuncture has a meaningful clinical effect on the improving shoulder pain, disability and ROM.

• Journal of Pharmacopuncture
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• v.14 no.4
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• pp.23-32
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• 2011

Objectives: This study was performed to examine the antigenic potential of pure melittin (Sweet Bee Venom - SBV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), authorized a non-clinical studies institution, under the regulations of Good Laboratory Practice (GLP). Antigenic potential of SBV was examined by active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) in guinea pigs. SBV was subcutaneously administered at 0.07 and 0.28mg/kg and also as a suspension with adjuvant (Freund's complete adjuvant: FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control response ($5mg/m{\ell}$-FCA). Results: 1. In the ASA test, experimental groups showed some symptoms of anaphylaxis like piloerection, hyperpnea and staggering gait. 2. In the PCA test, low dosage group did not show any antibody responses, whereas high dosage group showed positive responses. 3. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. 4. In the autopsy of body, the abnormalities of lung were detected in the corpse. This means that the cause of death may induced anaphylactic shock. Conclusions: Above findings suggested that SBV had antigenic potential in guinea pig. Further studies on the subject should be conducted to yield more concrete evidences.

• Journal of Pharmacopuncture
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• v.14 no.3
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• pp.47-53
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• 2011

Objectives: This study was performed to analyse the effects of Sweet Bee Venom(SBV-purified melittin supported by G&V Co., the major component of honey bee venom) on the respiratory system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice(GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, SBV was administered in thigh muscle of rats in 0.175, 0.35 and 0.7 mg/kg dosage. And checked the effects of SBV on the respiratory system using the whole body plethysmography. And respiratory rate, tidal volume and minute volume of rats were checked after administered SBV (melittin). Results: 1. In the measurement of respiratory rate, there were not observed any significant differences compared with control group. 2. In the measurement of tidal volume, there was not observed any significant differences compared with control group. 3. In the measurement of minute volume, 0.35mg/kg dosage group showed significant differences compared with control group. But we estimated that this result was caused by individual differences. Conclusions: Above findings suggest that SBV seems to be safe treatment in the respiratory system of rats. And further studies on the subject should be conducted to yield more concrete evidences.

• Journal of Pharmacopuncture
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• v.11 no.2
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• pp.55-62
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• 2008

Objective Sweet bee venom is made by removing allergen from the bee venom through gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis. The aim of this study was to verify allergy inhibitory action in Sweet Bee Venom(SBV) and New Sweet Bee Venom(NSBV) removed enzymes and compounds of low molecular weight. Methods 84 healthy adult men and women were selected through a survey whom had never received the bee venom therapy in the past. The concentration of Normal Saline, SBV and NSBV pharmacopuncture was equally at 0.1mg/mL and the experiment was conducted as the double blind test. Results Participants of the study was comprised of 63 men and 21 women with the average age of 28.3 years. According to results of pain sense, SBV group showed significant higher score compared with NS group and NSBV group using VAS in treating time. And SBV and NSBV group showed significant higher score compared with NS group after 30 minutes. Other allergic responses were insignificant between the groups. Conclusions As a result of removed allergen and compounds of low molecular weight, NSBV significantly inhibits pain sense in treating time compared with SBV. This indicates wider and easier application of NSBV for the useful application in clinical treatment. Further comparative studies should be conducted to yield more objective verification.

• Journal of Pharmacopuncture
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• v.2 no.1 s.2
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• pp.1-11
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• 1999

We have studied Bee Venom Therapy to treat Sequalae of Sprain, and obtained following results. 1. The treatment of sprain is using the effect of the affected part on Bee Venom Therapy. 2. For the treatment of sprain, it is often used the near acupuncture point of injury(近位取穴) or painful point to press(阿是穴). 3. Bee Venom Therapy is one of the powerful treatment, so we are using the sequalae of sprain in the case of not cured by Acupuncture, Cupping or another therapy. 4. Bee Venom injection is started a small quantity, and is increased slowly by degrees. 5. The immune response by Bee Venom Therapy is a sort of Allergy, and it is classified large delayed reaction. It is considered positive reaction. 6. We have selected Acupuncture Points from one to four by the degree of injury. 7. The period of treatment is some different from the degree of injury, but generally we have obtained good results to treat from 5 to 20 by Bee Venom Therapy.