• Title/Summary/Keyword: Batch method

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Nonthermal Sterilization of Dried Laver by Intense Pulsed Light with Batch System (회분식 광펄스 처리에 의한 마른 김의 비가열 살균)

  • Kim, Ae-Jin;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.778-781
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    • 2014
  • Intense pulsed light (IPL) is a nonthermal technology emerging as an alternative to conventional thermal treatment. The purpose of this study was to investigate the effect of IPL treatment on the microbial inactivation, color alteration, and temperature change of dried laver to evaluate the commercial feasibility of IPL as a sterilization method. IPL treatment (10 min at 1,000 V and 5 pps) resulted in approximately 1.6 log CFU/g decrease in microbial cell viability. After IPL treatment, the surface temperature of dried laver increased by $1.9^{\circ}C$. The color lightness of dried laver increased with increased treatment time, while redness and yellowness decreased. However, these color differences were not significant.

Production of Green Fluorescent Protein (GFP) from Transgenic Rice Cell Suspension Culture (형질전환된 벼세포배양에서 green fluorescent protein (GFP) 생산)

  • Lee, Jae-Hwa
    • Journal of Life Science
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    • v.17 no.2 s.82
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    • pp.293-297
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    • 2007
  • Green fluorescent protein (GFP) is an attractive reporter for bioprocess monitoring. A fluorescence-based method was developed to quantify GFP levels in transgenic plants and protein extracts. In this study, GFP was produced and secreted from suspension cells derived from transgenic rice. The RAmy3E promoter placed before the GFP gene controlled by sugars such as sucrose. The effects of sucrose concentration on the secretion of GFP and total protein into the medium were investigated in batch suspension culture. It was possible, therefore, to induce the expression of the GFP by removing sucrose from the cultured media or by allowing the rice suspension cells to deplete sucrose catabolically. The dry cell weight (7.06 g/L) and GFP level were detected as highest at 12%, 3% sucrose after 20 day culture, respectively. However secreted GFP fluorescence at the other sucrose concentrations (6%, 12%, 18% and 24%) were a little amount in media.

Fabrication of Thick SmBCO/IBAD-MgO coated conductor (후막 SmBCO/IBAD-MgO 초전도 박막선재의 제조)

  • Lee, J.H.;Kang, D.K.;Ha, H.S.;Ko, R.K.;Oh, S.S.;Kim, H.K.;Yang, J.S.;Jung, S.W.;Moon, S.H.;Youm, D.;Kim, C.J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.05a
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    • pp.9-9
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    • 2009
  • Coated conductor is required to have good critical current property for high efficiency of electric power applications. Until now, long coated conductor does not show high Jc over 3 MA/$cm^2$ in thick superconducting layer because of texture degradation by thick superconducting layer. In this study, in order to overcome this issue, thicker superconducting layer was deposited with optimized conditions to reduce the degradation of critical current density. SmBCO superconducting coated conductor was deposited with 1~3 um of thickness at $750\sim850^{\circ}C$ under 15~20 mTorr of oxygen partial pressure using batch type EDDC( evaporation using drum in dual chamber). The buffered substrate for superconducting layer deposition was used IBAD-MgO template with the architecture of $LaMnO_3/MgO/Y_2O_3/Al_2O_3$/Hastelloy. After fabrication of coated conductor, critical current was measured by 4-prove method under self-magnetic field and 77K. In addition, surface morphology and texture were analyzed by SEM and XRD, respectively. 3 um thick SmBCO coated conductor shows highest $I_C$ values of 638A/cm-w in 1 m long in the world.

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The Fabrication and Magnetoresistance of Nanometer-sized Spin Device Driven by Current Perpendicular to the Plane (수직전류 인가형 나노 스핀소자의 제조 및 자기저항 특성)

  • Chun, M.G.;Lee, H.J.;Jeung, W.Y.;Kim, K.Y.;Kim, C.G.
    • Journal of the Korean Magnetics Society
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    • v.15 no.2
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    • pp.61-66
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    • 2005
  • In order to make submicron cell for spin-injection device, lift-off method using Pt stencil and wet etching was chosen. This approach allows batch fabrication of stencil substrate with electron-beam lithography. It simplifies the process between magnetic film stack deposition and final device testing, thus enabling rapid turnaround in sample fabrication. Submicron junctions with size of $200nm{\times}300nm$ and $500nm{\times}500nm$ 500 nm and pseudo spin valve structure of $CoFe(30{\AA})/Cu(100{\AA})/CoFe(120{\AA}$) was deposited into the nanojunctions. MR ratio was 0.8 and $1.1{\%}$, respectively and spin transfer effect was confirmed with critical current of $7.65{\times}10^7A/cm^2$.

Adsorption Behavior of Monosubstituted-Halophenols by Amberlite XAD Resins (Amberlite XAD 수지에 대한 일치환 할로 페놀들의 흡착거동에 관한 연구)

  • Lee, Taek Hyeok;Lee, Dae Un
    • Journal of the Korean Chemical Society
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    • v.34 no.3
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    • pp.267-279
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    • 1990
  • The adsorption mechanisms of phenols on XAD-2 and XAD-7 resins were studied by using the distribution coefficient(log Kd) measured in the optimum adsorption conditions. It was observed that the Langmuir adsorption isotherm, indicating a molecular size-dependent adsorption, was appropriate for characterizing the adsorption behaviors of phenols on XAD-2 and XAD-7 resins. The adsorption energies of phenols on XAD resins were calculated by Lennard-Jones potential equation. In the calculation of the adsorption energy, the molecular radii and dipole moments of the resins and phenols were calculated by their van der Waals volumes and Debye equation, respectively. The stacking factor (F) were determined from the radio of the equilibrium distance to the stacking distance of molecules. In order to explain the adsorption energy calculated from the stacking factor it was compared with the adsorption enthalpy for each of phenols which was experimentally determined by batch adsorption shake method. It was observed that the adsorption enthalpy of phenolate ions on the XAD resins was likely to be more seriously affected by dispersion interaction than by a dipole or a charge interaction.

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Synthesis of Lithium Manganese Oxide by Wet Mixing and its Removal Characteristic of Lithium Ion (습식혼합에 의한 리튬망간 산화물의 합성과 리튬이온 제거특성)

  • You, Hae-Na;Lee, Dong-Hwan;Lee, Min-Gyn
    • Clean Technology
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    • v.19 no.4
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    • pp.446-452
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    • 2013
  • In this paper, the wet mixing method was introduced to prepare spinel lithium manganese oxide (LMO) with $Li_2CO_3$ and $MnCO_3$. The physical properties of the resulting lithium manganese oxide were characterized by the XRD and SEM. The adsorption properties of LMO for $Li^+$ were investigated by batch methods. The maximum adsorption capacity of lithium was calculated from Langmuir isotherm and found to be 27.25 mg/g. The LMO are found to have a remarkable lithium ion-sieve property with distribution coefficients ($K_d$) in the order of $Ca^{2+}$ < $K^+$ < $Na^+$ < $Mg^{2+}$ < $Li^+$, which is promising in the lithium extraction from seawater.

A Study about Analysis of Weld Distortion using Genetic Algorithm (유전적 알고리듬을 이용한 용접변형 해석에 관한 연구)

  • Kim, Ill-Soo;Kim, Hak-Hyoung;Jang, Han-Kee;Kim, Hee-Jin;Kwak, Sung-Kyu;Ryoo, Hoi-Soo;Shim, Ji-Yeon
    • Journal of Welding and Joining
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    • v.27 no.4
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    • pp.54-59
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    • 2009
  • In the process to manufacture for metallic structures, control of welding deformation is one of an important problems connected with reliability of the manufactured structures so that welding deformation should be measured and controlled with quickly and actively. Also, welding parameters which have as lot of effects on welding deformation such as arc voltage, welding current and welding speed can also be controlled. The objectives for this study were to develop a simple 2-D FEM to calculate not only the transient thermal histories but also the sizes of fusion and heat-affected zone (HAZ) in multi pass arc welds including the butt and fillet weld type with dissimilar thickness, and to concentrate on a developed model for the finding the parameters of Godak's moving heat source model based on a GA. The developed model includes a GA program using MATLB and GA toolbox, and a batch mode thermal model using ANSYS software. Not only the thermal model was verified by comparison with Goldak's work but also the developed model was validated with molten zone section experimental data.

Biodesulfurization of Dibenzothiophene and Its Derivatives Using Resting and Immobilized Cells of Sphingomonas subarctica T7b

  • Gunam, Ida Bagus Wayan;Yamamura, Kenta;Sujaya, I. Nengah;Antara, Nyoman Semadi;Aryanta, Wayan Redi;Tanaka, Michiko;Tomita, Fusao;Sone, Teruo;Asano, Kozo
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.473-482
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    • 2013
  • The desulfurization ability of Sphingomonas subarctica T7b was evaluated using resting and immobilized cells with dibenzothiophene (DBT), alkyl DBTs, and commercial light gas oil (LGO) as the substrates. The resting cells of S. subarctica T7b degraded 239.2 mg of the initial 250 mg of DBT/l (1.36 mM) within 24 h at $27^{\circ}C$, while 127.5 mg of 2-hydroxybiphenyl (2-HBP)/l (0.75 mM) was formed, representing a 55% conversion of the DBT. The DBT desulfurization activity was significantly affected by the aqueous-to-oil phase ratio. In addition, the resting cells of S. subarctica T7b were able to desulfurize alkyl DBTs with long alkyl chains, although the desulfurization rate decreased with an increase in the total carbon number of the alkylated DBTs. LGO with a total sulfur content of 280 mg/l was desulfurized to 152 mg/l after 24 h of reaction. Cells immobilized by entrapment with polyvinyl alcohol (PVA) exhibited a high DBT desulfurization activity, including repeated use for more than 8 batch cycles without loss of biodesulfurization activity. The stability of the immobilized cells was better than that of the resting cells at different initial pHs, higher temperatures, and for DBT biodesulfurization in successive degradation cycles. The immobilized cells were also easily separated from the oil and water phases, giving this method great potential for oil biodesulfurization.

DESTRUCTION OF HUMIC MATTERS AND AMMONIA IN THE LANDFILL LEACHATE BY SUPERCRITICAL WATER OXIDATION

  • Kim, Y.K.;Ahn, J.S.;Leung, W.
    • Environmental Engineering Research
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    • v.11 no.6
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    • pp.311-317
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    • 2006
  • Feasibility of destroying synthetic and actual leachate containing humic acids and ammonia compounds by supercritical water oxidation (SCWO) was evaluated. In this study, destruction efficiencies of humic acids and ammonia respectively were investigated at various reaction temperatures and residence times under pressure a supercritical pressure (280 atm). To lower reaction temperature, chemical oxidants were used. The experiment was carried out in a cylindrical batch reactor made of Hastelloy C-276 that can withstand high temperature and pressure. Concentrations of humic acids and ammonia were measured using a $COD_{Cr}$ method and an ammonia selective electrode, respectively. The optimal destructive condition of humic acids in the presence of stoichiometric oxygen(air) was 3 min at $380^{\circ}C$, but the temperature could be lowered to subcritical region ($360^{\circ}C$) along with $H_2O_2$ as an oxidant. For ammonia, the optimal destructive condition with air was 5 min at $660^{\circ}C$, but it was possible to operate the process for 3 minutes at $550^{\circ}C$ or 2 min at $600^{\circ}C$ along with $H_2O_2$ as an oxidant. At 2 min and $550^{\circ}C$ along with $H_2O_2$ as an oxidant, humic and ammonia compounds in the actual leachate were easily destructed and the effluent quality met the Korea Standard Leachate Quality.

Processing of Protein Concentrate and Fatty Acid Extraction from Tuna Viscera using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 가다랑어 내장으로부터 지방산 추출 및 단백질 농축물의 제조)

  • CHUN Byung-Soo;YOON Sung-Ok;LEE Seung-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.207-212
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    • 2001
  • Supercritical fluid extraction was explored as a method for removing lipids and bad odor from tuna viscera. Selected conditions of extraction pressure, time, temperature and sample size were evaluated for effective removal of lipids and bad odor, Supercritical carbon dioxide was used as a solvent and the extraction was performed at semi-batch flow type. The experimental conditions used in this work was the range of pressure from 1,500 psig to 2,000 psig, the temperature from $25^{\circ}C\;to\;40^{\circ}C$ and dried sample size from 0.2 mm to 1.0 mm. The main fatty acids extracted from tuna viscera were palmitic acid (16: 0) heptadecenoic acid (17: 1) oleic acid (18: 1) and docosahexaenoic acid (22: 6). Protein concentrate was obtained without deformation the optimum condition at $35^{\circ}C$, 1,800 psig and 0.25 mm of the size. In the concentrate after supercritical carbon dioxide extraction, the major amino acids were glutamic acid, leucine and lysine.

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