• Journal of environmental and Sanitary engineering
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• v.11 no.2
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• pp.1-7
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• 1996

The behavior of total organic carbon (TOC) and phosphate were observed for 15 days with immobilized activated sludge using polyacrylamide (PAA) by sequencing batch reactor (SBR). In the preparation of immobilized sludge by PAA, it was found that suitable acrylamide concentration for actual wastewater treatment was to be 15% through the batch test. When SBR system was operated in the repeated aerobic and anaerobic conditions, TOC removal efficiency was 92%. The uptake rate of phosphate was increased from 1.78 mg-P/g cell/hr on the 5th day of acclimation to 2.5 mg-P/g cell/hr on the 15th day of acclimation. And the total phosphorus content in PAA bead was increased from 40 mg-P/g cell on the 1st day of operation to 55 mg-P/g cell on the 15th day of operation. From this study, lowering the volume of aeration tank was possible when PAA bead was used in wastewater treatment and long operation was also possible without the settler.

• Journal of Microbiology and Biotechnology
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• v.7 no.5
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• pp.323-328
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• 1997

The recombinant Escherichia coli BL21(DE3)pLysE : pTCGT1 was grown to overproduce Bacillus macerans cyclodextrin glucanotransferase (CGTase) able to synthesize ${\alpha}$-cyclodextrin (CD) with a selectivity of 67%. A number of batch fermentations were performed to test the possibility of using lactose as an inducer of the E. coli T7 promoter system. A mixture of isopropyl ${\beta}$-D-thiogalactoside (IPTG) and lactose (1 : 1) gave a maximum CGTase activity of 2.4 U/ml, which was higher than the value obtained with induction by IPTG alone. Fed-batch fermentations involving a glucose-controlled growth period followed by a gene-expression phase with mixtures of IPTG and lactose were employed to achieve high cell density and thereby increase total CGTase activity. Optimized fed-batch fermentation using the modified inducer (IPTG : lactose=1 : 3) and 100 g/l yeast extract solution in the gene-expression phase resulted in a maximum CGTase activity of 62.9 U/ml and a final cell mass of 53.5 g/l, corresponding to a 31-fold increase in CGTase activity and a 29-fold increase in cell mass compared with the control batch fermentation.

• The Transactions of the Korea Information Processing Society
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• v.4 no.7
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• pp.1900-1906
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• 1997

In this paper, the efficient BILBO(named EBILBO) is designed for BIST that is able to batch the testing when circuit is designed on FPGA. The proposed algorithm of batch testing is able to test the normal operation speed with one-pin-count that can control all part of large and complex circuit. PRTPG is used for the test pattern and MISR is used for PSA. The proposed algorithm of batch testing is VHDL coding on behavioral description, so it is easily modified the model of test pattern generation, signature analysis and compression. The EBILBO's area and the performance of designed BIST are evaluated with ISCAS89 benchmark circuit on FPGA. In circuit with above 600 cells, it is shown that area is reduced below 30%, test pattern is flexibly generated about 500K and the fault coverage is from 88.3% to 100%. EBILBO for the proposed batch testing BIST is able to execute concurrently normal and test mode operation in real time to the number of $s+n+(2^s/2^p-1)$ clock(where, in CUT, # of PI;n, # of register, p is order # of polynomial). The proposed algorithm coded with VHDL is made of library, then it well be widely applied to DFT that satisfy the design and test field on sme time.

• Journal of Drive and Control
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• v.15 no.3
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• pp.43-48
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• 2018

The hydraulic control valve, used in the CPB (cold-pad-Batch) cold dyeing system, passes through a pressurized material that absorbs the dye. The hydraulic control of the hydraulic control panel shall be driven in a uniform and precisely controlled manner, as it interferes directly with the dyschromatism. In this study, an acceleration test model was employed to verify the durability of the hydraulic control of the hydraulic control panel, which was manufactured by the scenic model, and the pre-roll angle was analyzed before the performance of acceleration test. Based on the change in the amount of deformation of the padder roll the durability of the padder roll was analyzed along with verification of the durability of the skin and the rubber coating in contact with the fabric. Furthermore, the accelerated test method used for hydraulic controlled multi-cell padder rolls was verified.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.2
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• pp.225-234
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• 2006

Characteristics of electricity production from major fermentation products (acetate, propionate and butyrate) were evaluated in a microbial fuel cell (MFC). For each substrate, batch and continuous experiments were performed. The batch test result indicated that coulombic efficiency depended on the resistance connected in MFC circuit. With acetate, coulombic efficiency were 87% at $20{\Omega}$, but decreaced to 45% at$220{\Omega}$. In continuous tests, maximum power densities obtained was 220 Q with acetate. The maximum power densities of butyrate, acetate and propionate were 6.8, 6.1, and $5.2mW/m^2$, respectively. Propionate and butyrate were converted into acetate producing high currents. $H_2$ produced during butyrate and propionate probably used to produce electricity. In conclusion, butyrate conversion into acetate was faster than that of propionate with higher electricity production. If the production of propionate is inhibited during fermentation, anaerobically fermented liguor may be effectively applied for MFC.

• Environmental Engineering Research
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• v.10 no.2
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• pp.79-87
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• 2005

The effects of transformation capacity on cometabolic degradation of trichloroethene (TCE) were evaluated using TCE-degrading actinomycetes pure and mixed culture under various culture conditions. The TCE transformation capacity of the actinomycetes enrichment culture in a batch test with phenol addition was 1.0 mg of TCE/mg of volatile suspended solids (VSS). The resting cell TCE transformation capacity of the actinomycetes pure culture cell was 0.75 mg TCE/mg VSS, which increased to 2.0 mg TCE/mg VSS when phenol was added as an external substrate. When the pure culture had an internal substrate in the form of poly-β-hydroxybutyrate (PHB) at 19% of the cell mass, the resting cell TCE transformation capacity increased from 0.47 to 0.6 mg TCE/mg VSS. The presence of PHB increased transformation capacity by 57%, whereas, the addition of phenol caused more than two fold increase in transformation capacity. The actinomycetes culture showed the highest transformation capacity.

• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.212-219
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• 1991

Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis flbuligera, and Schwanniomyces castellii were evaluated for their ability to bioconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. castellii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25 $h^{-1}$. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23~38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.

• KSBB Journal
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• v.5 no.3
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• pp.307-313
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• 1990

A microcomputer-aided fermentation system was constructed for high density fed-batch culture using dissolved oxygen(DO) as a substrate feeding indicator. DO signal was processed prior to aquisition to computer. Agitation speed and oxygen flow rate was changed stepwisely to maintain DO value at a constant level. Agitation speed was controlled by the output signal of D/A converter. Oxygen flow rate was controlled by a flow rate control valve connected to a stepping motor. Substrate was fed with a feeding pump operated by the abrupt increase of DO signal. Methylobacillus sp. SK1 was cultivated to test the system and 16.53g/l of cell density was obtained after 10 hr.

• KSBB Journal
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• v.7 no.4
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• pp.296-301
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• 1992

A Pseudomonas fluorescens was selected from mushrooms and studied in both batch and fed-batch cultures in order to get maximal biomass concentration. P. fluorescens is an aerobic bacterium and antagonistic to Pseudomonas tolaasii which causes blotch disease on the mushroom cap. P fluarescens and P. tolaasii were identified by Gram staining, gelatin liquefaction, oxidase test, etc. and were characterized by pigment production, temperature sensitivity, salt tolerance and rapid pitting test, etc., Celts of P. fluorescens well in medium containing 30g/L of glucose, whereas the growth was inhibited at the glucose levels at higher than 30g/L. The highest values of specific growth rate and productivity were obtained when using 10g/1 of yeast extract. Optimum concentrations of $NH_4Cl$ and ${(NH_4Cl)}_2SO_4$ for culture were found to be 1.0g/L and 0.1g/L respectively. Optimum concentration of $MgSO_4{\cdot}7H_2O$ used as a sulfursource was 1.0g/L. It was also found that the cell concentrations reached the maximum level when grown on the medium containing 1.0g/L of $KH_2PO_4$ and 0.1g/L of $CaCl_2$. Also, the optimum culture conditions were $30^{\circ}C$ and pH 6.0. Cultivation of P. fluarescens at high dissolved oxygen (DO) concentration led to a decrease of bacterial productivity in batch culture. Maximum productivity was achieved at 40% DO concentration.

• Journal of Pharmaceutical Investigation
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• v.35 no.3
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• pp.207-224
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• 2005

The objective of this study was to explore the principles of SUPAC-SS and its regulatory application in handling postapproval changes to nonsterile semisolid dosage forms. The types of postapproval changes that SUPAC-SS described were modifications in formulation (components and composition), batch size, manufacturing equipment & process, and the site of manufacturing. SUPAC-SS defined the levels of postapproval changes and what chemistry, manufacturing, and control tests should be conducted for each change level. The guidance also specified several occasions the manufacturers should perform in vitro release test (Franz cell diffusion test) and/or in vivo bioequivalence test. Finally, SUPAC-SS classified appropriate filing forms to be used in supporting postapproval changes. It was crystal clear that SUPAC-SS helped maintain the safety and quality of approved semisolid dosage forms when they were subject to certain postapproval changes. The availability of SUPAC-SS made contributions to reducing regulatory burdens of the industry, as well as expediting the postapproval process of regulatory agencies. This study also shed light on the background of relevant pharmaceutical sciences that the SUPAC-SS guidance adopted. Finally, the KFDA and the industry were strongly urged to implant a similar guidance in handling postapproval changes to semisolid dosage forms available in the Korean marketplace.