• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2192-2198
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• 2016

The myeloid-specific IgA Fc receptor ($Fc{\alpha}R$) is a cell surface molecule on immunocytes that provides a fundamental connection between humoral and cellular immunity. In this study, the full-length cDNA sequence of swine $Fc{\alpha}RI$ ($swFc{\alpha}RI$) was isolated and characterized and found to contain a 792-base-pair open reading frame, encoding a 264-amino-acid transmembrane glycoprotein with a predicted molecular mass of 29.4 kDa. The $swFc{\alpha}RI$ shares high amino acid sequence homology (>50%) with its counterparts from cattle, seal, and horse. Rosetting analysis confirmed that COS-7 cells transfected with an $swFc{\alpha}RI$ expression plasmid was able to combine with chicken erythrocytes sensitized with porcine IgA, but not IgG.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.309-316
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• 1990

The sequence of a 1795 bp restriction fragment containing the B. circulans F-2 gene for NaC1- dependent $\alpha$-amylase (CI-amylase) is reported. The probable coding region of the gene is 1005 base pairs (335 amino acida) long. The NaC1-dependent $\alpha$-amylase (el-amy) sequence shows an open reading frame (ORF) with the translated molecular weight of about 38, 006, which correspond to a molecular weight of about 35, 000 (Mi). The gene is preceded by the sequence resembling promoter for the vegetative B, subtitis RNA polymerases. These are followed by the sequences resembling a B. subtilis ribosome binding site 5 nucleotides before the first codon of the gene. Homologous regions with other amylases were found. The N-terminal sequences of the mature proteins expressed in E. eoli were identical to the N-terminal sequences which are anaIysed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.10
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• pp.1219-1227
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• 2013

A new type of wearable robot is developed for a disabled person with disabled limbs, that is, a person who cannot intentionally move his/her legs and arms. This robot can enable the disabled person to grip an object using eye movements. A gaze tracking algorithm is employed to detect pupil movements by which the person observes the object to be gripped. By using this gaze tracking 2D information, the object is identified and the distance to the object is measured using a Kinect device installed on the robot shoulder. By using several coordinate transformations and a matching scheme, the final 3D information about the object from the base frame can be clearly identified, and the final position data is transmitted to the DSP-controlled robot controller, which enables the target object to be gripped successfully.

• Journal of Korean Society of Rural Planning
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• v.24 no.3
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• pp.1-12
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• 2018

This study aims to offer base line data for efficient conflicts control measures by first, analyzing the current situation of the regional comprehensive development project and second, identifying level, type and cause of the conflicts appeared during the development business promotion. For this purpose, theoretical side of this study establishes a basic frame by reviewing the existing researches in order to analyze the characteristics of the conflicts between the participation subjects in the development business, and the empirical side conducts survey and analysis on the awareness on these conflicts. As a result of the analysis, the survey revealed the followings. First, the respondents were aware of the overall promotions of the business quite positively, second, the conflicts were considered as a major obstacle against the development project promotion just as the other negative factors, third, majority of the responses on the seriousness of the conflicts were neutral which meant that the conflicts could be deepened by situations, fourth, most respondents had positive recognition on the needs of conflict control training and its effectiveness, and last but not least, most respondents of the survey revealed their intentions to take parts in trouble shooting from the conflicts. Based on the results above, we were able to extract several elements to consider preparing the countermeasures for the conflicts. First, specific plan should be arranged and institutionalized in order to control the conflicts between the residents at the initial stage of the development business or even before. Second, the specific plan must lower its threshold for easy participation of the residents in that region and make the residents main body of the conflict control. Third, enhanced conflict control education must be provided to the residents of the region where comprehensive development plan is in progress or being prepared.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.10B
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• pp.911-918
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• 2008

In this paper, we propose new cell structures using multi-hop Relay Station(RS) based on IEEE802.16j in Narrow-Beam Trisector Cell (NBTC) and Wide-Beam Trisector Cell (WBTC), which are two methods for cell sectorization using 3-sector directional antennas. Then, we analyze our proposed structures compared with the existing system which does not use any RS about the numbers of optimized Base Station (BS) and multi-hop relay, the extended BS coverage, and the deployment cost according to the traffic density using optimization model. According to the results, we know the reduction of total deployment cost of the proposed systems and that WBTC is suitable when the traffic density is high and NBTC is suitable when the traffic density is low in our proposed multi-hop based NBTC and WBTC structures.

• Journal of Ginseng Research
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• v.34 no.4
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• pp.296-303
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• 2010

A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.5
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• pp.397-404
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• 2017

The purpose of this study is to evaluate the seismic performance of Staggered Truss Frame(STF) system while changing a shape of truss. The model of this project is a office building of ten floors with Pratt, Howe, Warren, K and Vierendeel truss system applied on each model. Next step is to select the section of elements which satisfy the highest demand capacity ratio by structure design considering gravity load, earthquake load and wind load and then calculate natural period, base shear and story drifts. On the basis of these values, Capacity Spectrum Method(CSM) shows the plastic behavior of STF system such as performance point of Design Earthquake(DE) and Maximum Considered Earthquake(MCE), yield state, plastic hinge etc. to be compared with other truss systems. As a result, Vierendeel STF system especially was found to have the highest strength and stiffness to the corresponding earthquake and all the models for each truss shape fulfilled the target performance level.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.273-277
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• 2004

$\alpha$-L-Arabinofuranosidase ($\alpha$-L-AFase, EC 3.2.1.55) was isolated from hyperthermophilic microorganism, Thermotoga maritima. The open reading frame (ORF) of $\alpha$-L-AFase gene is 1,455 bp long and encodes 484 amino acid residues with a molecular weight of 55,265 Da. The ORF of $\alpha$-L-AFase gene was introduced into the E. coli expression vector, $_p/RSET-B, and overexpressed in E. coli BL21. The purified recombinant $\alpha$-L-AFase showed the highest activity at 10$0^{\circ}C$ and pH 5.5. The purified enzyme appeared to have no metal cofactor requirement. The Km and specific activity values of the recombinant enzyme were 0.99 mM and 1,200 U/mg on p-nitrophenyl-$\alpha$-L-arabinofuranoside. It released only L-arabinose from sugar beet arabinan, sugar beet debranched arabinan and oat spelts arabinoxylan but had no activity onarabinogalactan and gum arabic. This result suggests that L-arabinose could be produced from natural polysaccharides using this enzyme. Mutant enzymes which Glu26, Glu172 and Glu281 residues were replaced to alanine, aspartic acid or glutamine caused Kcat to decrease by a factor of between 10$^3$ and 10$^4$. Glu172 and Glu281 residues of $\alpha$-L-AFase are seemed to be the acid/base and nucleophile in catalytic reaction, respectively, and Glu26 is supposed to playa key role in substrate binding.ng.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1653-1663
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• 2010

The first gene (${\alpha}$-gal1) encoding an extracellular ${\alpha}$-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The ${\alpha}$-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a 24 amino acid secretory signal sequence. The translated protein had highest identity with other fungal ${\alpha}$-galactosidases belonging to glycosyl hydrolase family 27. The ${\alpha}$-gal1 gene was overexpressed as a secretory protein with an N-terminal histidine tag in the methylotrophic yeast Pichia pastoris. Recombinant ${\alpha}$-Gal1 was secreted into the culture medium as a monomeric glycoprotein with a maximal yield of 10.75 mg/l and purified to homogeneity using Hisbinding nickel-agarose affinity chromatography. The purified enzyme was maximally active at $70^{\circ}C$, pH 4.5, and lost no activity over 10 days at $50^{\circ}C$. ${\alpha}$-Gal1 followed Michaelis-Menten kinetics ($V_{max}\;of\;240.3{\mu}M/min/mg,\;K_m\;of\;0.294 mM$) and was inhibited competitively by galactose ($K_m{^{obs}}$ of 0.57 mM, $K_i$ of 2.77 mM). The recombinant T. emersonii ${\alpha}$-galactosidase displayed broad substrate preference, being active on both oligo- and polymeric substrates, yet had strict specificity for the ${\alpha}$-galactosidic linkage. Owing to its substrate preference and noteworthy stability, ${\alpha}$-Gal1 is of particular interest for possible biotechnological applications involving the processing of plant materials.

• Journal of the Korean Society for Railway
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• v.20 no.4
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• pp.440-447
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• 2017

Spectrum level for the acoustic roughness of wheels and rail surface should be periodically maintained under the limitation of ISO to reduce rolling noise of railway vehicles. Thus, in maintaining railway track, displacement sensor-based measuring devices are broadly used to measure the surface roughness and to perform spectral analysis. However, these measuring devices cause unexpected measuring errors since the displacement sensors are fixed at moving platforms and the main frame produces pitching motion during measurement. To increase the accuracy of the measured values, this paper has investigated the effects of design variables such as wheel base, additional wheels, and elastic deformation of wheels on the surface roughness and acoustic roughness spectrum.