• BMB Reports
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• v.39 no.5
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• pp.626-635
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• 2006

Lysophosphatidic acid acyltransferase (LPAAT) is an intrinsic membrane protein that catalyzes the synthesis of phosphatidic acid (PA) from lysophosphatidic acid (LPA). It is well known that LPAAT is involved in lipid biosynthesis, while its role in tumour progression has been of emerging interest in the last few years. To date, seven members of the LPAAT gene family have been found in human. Here we report a novel LPAAT member, designated as LPAAT-theta, which was 2728 base pairs in length and contained an open reading frame (ORF) encoding 434 amino acids. The LPAAT-theta gene consisted of 12 exons and 11 introns, and mapped to chromosome 4q21.23. LPAAT-theta was ubiquitously expressed in 18 human tissues by RT-PCR analysis. Subcellular localization of LPAAT-theta-EGFP fusion protein revealed that LPAAT-theta was distributed primarily in the endoplasmic reticulum (ER) of COS-7 cells. Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.576-584
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• 2002

Bovine PAS-4 is an integral membrane glycoprotein expressed in mammary epithelial cells. Complementary DNA (cDNA) cloning of PAS-4 was performed by reverse-transcriptase polymerase chain reaction (RT-PCR) with oligonucleotide probes based on it's amino terminal and internal tryptic-peptides. The cloned PAS-4 cDNA was 1,852 nucleotides (nt) long and its open reading frame (ORF) was encoded 1,413 base long. The deduced amino acid sequence indicated that PAS-4 consisted of 471 amino acid residues with molecular weight of 52,796, bearing 8 potential N-glycosylation sites and 9 cysteine residues. Partial bovine CD36 cDNA from liver also was sequenced and the homology of both nucleotide sequence was 94%. Most of the identical amino acid residues were in the luminal/extracellular domains. Contrary to PAS-4, bovine liver CD36 displays 6 potential N-glycosylation sites, which were located, except for those at positions 101 and 171, at same positions as PAS-4 cDNA. Cysteine residues of PAS-4 and CD36 were same at position and in numbers. Northern blot analysis showed that PAS-4 was widely expressed, although its mRNA steady-state levels vary considerably among the analyzed cell types. PAS-4 possessed hydrophobic amino acid segments near the amino- and carboxyl-termini. Two short cytoplasmic tails of the amino- and carboxyl-terminal ends constituted of a 5-7 and 8-11 amino acid residues, respectively.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.2
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• pp.24-31
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• 2011

This paper proposes spatio-temporal denoising algorithm based on nonlocal means. Though the conventional denoising algorithms based on nonlocal means have good performance in noise removal, it is difficult to implement them into the hardware system due to much computational load and the need for several frame buffers. Therefore we adopted infinite impulse response temporal noise reduction algorithm in the proposed algorithm. Proposed algorithm shows less artificial denoised result in the motionless region. In the motion region, spatial filter based on efficiently improved nonlocal means algorithm conduct noise removal with less motion blur. Experimental results including comparisons with conventional algorithms for various noise levels and test images show the proposed algorithm has a good performance in both visual and quantitative criteria.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.18 no.2
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• pp.181-190
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• 2005

In the conventional outrigger system, the outriggers are located in the planes of the core walls and this system has disadvantage of obstructing flexibility in the interior layout. But thc facade riggers in the structure uc located In the exterior frames in the direction of the lateral loading. The interaction between the traced frames and facade riggers is through the floor diaphragms adjacent to the chords of the riggers. This paper presents an approximate analysis technique lot preliminary analysis of multiple facade rigger stiffened braced frames in tall buildings subjected to uniformly and triangularly distributed loads as well as a lateral point load at the top of the structure. Comparisons with the results by the program MIDAS for the structural models have shown that this analysis can give reasonably accurate results for highrise braced frames with multiple facade riggers. The method allows a simple procedure for obtaining the optimum level of the facade riggers in addition to a rapid assessment of the influence of the facade riggers on the performance of the highrise structure such as the reduction in lateral deflection at the top and the overturning moment at the base of the braced frame.

• Archives of Plastic Surgery
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• v.33 no.4
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• pp.510-513
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• 2006

Purpose: The Rectus abdominis muscle free flap is utilized in various reconstruction surgeries due to easiness in harvesting, consistency of vascular pedicle and reduced donor site morbidity. But rarely, femoral nerve injury during rectus abdominis harvesting can be resulted. We report a case of femoral nerve injury after rectus muscle harvesting and discuss the injury mechanism with the follow-up process of this injury. Methods: To reconstruct the defect of middle cranial base after wide excision of cystic adenocarcinoma of the external ear, rectus muscle free flap was havested in usual manner. To achieve a long vessel, inferior epigastric artery was dissected to the dividing portion of femoral artery and cut. Results: One week after the surgery, the patient noted sensory decrease in the lower leg, weakness in muscle strength, and disabilities in extension of the knee joint resulting in immobilization. EMG and NCV results showed no response on stimulation of the femoral nerve of the left leg, due to the defects in femoral nerve superior to the inguinal ligament. With routine neurologic evaluations and physical therapy, on the 75th day after the operation, the patient showed improvement in pain, sensation and muscle strength, and was able to move with walking frame. In 6 months after the operation, recovery of the muscle strength of the knee joint was observed with normal flexion and extension movements. Conclusion: Rarely, during dissection of the inferior epigastric artery, injuries to the femoral nerve can be resulted, probably due to excessive traction or pressure from the blade of the traction device. Therefore, femoral nerve injury can be prevented by avoiding excessive traction during surgery.

• Journal of Ocean Engineering and Technology
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• v.29 no.4
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• pp.322-330
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• 2015

As a new technical approach, a hydraulic and magnetic clamp device was developed to realize a magnetic clamp crane system by simultaneously actuating eight individual hydraulic cylinders. In this approach, an Sr-type of ferritic permanent magnet (SrO· 6Fe₂O₃), rather than the previous electromagnet, was utilized for the purpose of lifting and transporting the large curved steel plates used for manufacturing ships. This study had the goal of developing and manufacturing a hydraulic, magnetic clamp prototype composed of three main parts, including the base frame, cylinder joint, and magnet joint, in order to safely transport curved steel plates. Furthermore, this research included a performance evaluation of the manufactured prototype and acquired the purposed quantity value in the performance test. The most significant item, the magnetic adhesive force (G), was evaluated in a performance test, which utilized a ferritic permanent magnet (Sr type) with 3700~4000 G of residual induction (Br) and 2640/2770 Oe of coercive force (Hc). In particular, relevant items such as the hoist tension (kN), transportation time (s), and applied load (Kgf) on the hydraulic cylinders were also evaluated in order to determine the optimum values.

• Earthquakes and Structures
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• v.18 no.1
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• pp.27-44
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• 2020

This work presents a comparison of three performance-based seismic design methods (PBSD) as applied to plane steel frames having eccentric braces (EBFs) and buckling restrained braces (BRBFs). The first method uses equivalent modal damping ratios (ξ_k), referring to an equivalent multi-degree-of-freedom (MDOF) linear system, which retains the mass, the elastic stiffness and responds in the same way as the original non-linear MDOF system. The second method employs modal strength reduction factors (${\bar{q}}_k$) resulting from the corresponding modal damping ratios. Contrary to the behavior factors of code based design methods, both ξ_k and ${\bar{q}}_k$ account for the first few modes of significance and incorporate target deformation metrics like inter-storey drift ratio (IDR) and local ductility as well as structural characteristics like structural natural period, and soil types. Explicit empirical expressions of ξ_k and ${\bar{q}}_k$, recently presented by the present authors elsewhere, are also provided here for reasons of completeness and easy reference. The third method, developed here by the authors, is based on a hybrid force/displacement (HFD) seismic design scheme, since it combines the force-base design (FBD) method with the displacement-based design (DBD) method. According to this method, seismic design is accomplished by using a behavior factor (q_h), empirically expressed in terms of the global ductility of the frame, which takes into account both non-structural and structural deformation metrics. These expressions for q_h are obtained through extensive parametric studies involving non-linear dynamic analysis (NLDA) of 98 frames, subjected to 100 far-fault ground motions that correspond to four soil types of Eurocode 8. Furthermore, these factors can be used in conjunction with an elastic acceleration design spectrum for seismic design purposes. Finally, a comparison among the above three seismic design methods and the Eurocode 8 method is conducted with the aid of non-linear dynamic analyses via representative numerical examples, involving plane steel EBFs and BRBFs.

• Structural Engineering and Mechanics
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• v.61 no.4
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• pp.527-538
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• 2017

The evaluation of the loss-of-support conditions of frictional beam-to-column connections using simplified numerical models describing the transverse response of a portal-like structure is presented in this paper considering the effects of the seismic-hazard disaggregation. Real earthquake time histories selected from European Strong-motion Database (ESD) are used to show the effects of the seismic-hazard disaggregation on the beam loss-of-support conditions. Seismic events are classified according to different values of magnitudes, epicentral distances and soil conditions (stiff or soft soil) highlighting the importance of considering the characteristics of the seismic input in the assessment of the loss-of-support conditions of frictional beam-to-column connections. A rigid and an elastic model of a frame of a precast industrial building (2-DoF portal-like model) are presented and adopted to find the minimum required friction coefficient to avoid sliding. Then, the mean value of the minimum required friction coefficient with an epicentral distance bin of 10 km is calculated and fitted with a linear function depending on the logarithm of the epicentral distance. A complete parametric analysis varying the horizontal and vertical period of vibration of the structure is performed. Results show that the loss-of-support condition is strongly influenced by magnitude, epicentral distance and soil conditions determining the frequency content of the earthquake time histories and the correlation between the maxima of the horizontal and vertical components. Moreover, as expected, dynamic characteristics of the structure have also a strong influence. Finally, the effect of the column nonlinear behavior (i.e. formation of plastic hinges at the base) is analyzed showing that the connection and the column are a series system where the maximum force is limited by the element having the minimum strength. Two different longitudinal reinforcement ratios are analyzed demonstrating that the column strength variation changes the system response.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.417-421
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• 2004

A normal prion protein (PrPc) is converted to a protease resistant isoform (PrPsc) by an apparent self-propagating activity in bovine spongiform encephalopathies (BSE), which is a neurodegenerative disease. The cDNA encoding bovine PrP open reading frame (ORP) in Korean cattle was cloned by polymerase chain reaction (PCR). The cloned cDNA had a length of 795 base pairs which coded for a protein of 264 amino acid residues with a calculated molecular mass of 28.6 kDa. Identities of 90, 90, 79 and 78% on nucleotide and 94, 94, 84, and 84% on amino acid sequence were shown to PrP genes from sheep, goat, human, and mouse, respectively. The cloned DNA was ligated into the pQE30 expression vector and transformed into E. coli M15. The PrP was expressed by induction with isopropyl-$\beta$-D-thiogalactoside (IPTG) and purified on the Ni-NTA affinity column. High specific activities of the recombinant PrP were observed in the fraction of pH 5.8 eluate and showed a molecular mass of-29 kDa on SDS-PAGE and Western blot analysis.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.160-166
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• 2008

A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.