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Effects of Demineralized Bone Particle Loaded Poly(lactic-co-glycolic acid) Scaffolds on the Attachment and Proliferation of Costal Cartilage Cells (탈미네랄화된 골분/PLGA 지지체에서 늑연골 세포의 부착과 성장에 미치는 영향)

  • Cho, Sun Ah;Song, Jeong Eun;Kim, Kyoung Hee;Ko, Hyun Ah;Lee, Dongwon;Kwon, Soon Yong;Chung, Jin Wha;Khang, Gilson
    • Polymer(Korea)
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    • v.37 no.5
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    • pp.632-637
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    • 2013
  • It has been widely accepted that costal cartilage cells (CCs) have more excellent initial proliferation capacity than articular cartilage cells as well as the easiness for isolation and collection. This study demonstrated that CCs might be one of the substitutes for articular cartilage cells by tissue engineered cartilage. Poly(lactic-co-glycolic acid) (PLGA) has been extensively tested and used as scaffold material but it was limited by the low attachment of cells and the induction of inflammatory cells. Base on previous our studies, we confirmed demineralized bone particle (DBP) had the power of the reduction of inflammatory reaction and the stimulation proliferation of cells. We fabricated PLGA scaffold loaded with 10, 20, 40 and 80 wt% DBP and then tested the possibility of the regeneration of cartilage using CCs. Assays of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scanning electron microscope (SEM) carried out to evaluate the attachment and proliferation of CCs in DBP/PLGA scaffolds. Glycosaminoglycan (sGAG) and collagen contents assay were conducted to confirm the effects of DBP on formation of extracellular matrix. This study demonstrated that DBP/PLGA scaffolds showed significant positive effects on cell growth and proliferation due to the vitality of DBP as well as the possibility of the application of CCs for tissue engineered cartilage.

In vitro study of compressive fracture strength of Empress 2 crowns cemented with various luting agents

  • Kim Min-Ho;Yang Jae-Ho;Lee Sun-Hyung;Chung Hun-Young;Chang Ik-Tae
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.3
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    • pp.260-272
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    • 2001
  • All-ceramic restorations have had a more limited life expectancy than metal ceramic restorations because of their low strength. Their relatively lower strength and resistance to fracture have restricted the use of all-ceramic crowns to anterior applications where occlusal loads are lower. But there has been increasing interest in all-ceramic restorations because patients are primarily concerned with improved esthetics. Many efforts have been made to in prove the mechanical properties of dental ceramics. This study was designed to elucidate the influence of the luting agent on the strength of the Empress 2 crown (staining technique) cemented on human teeth. Seventy extracted human permanent molar teeth were chosen. Teeth were prepared for Empress 2 crowns with milling machine on a surveyor. A dental bur was placed in the mandrel that was positioned so that the long axis of the bur was perpendicular to the surveyor base. Dimensions of the Empress 2 crown preparation were $6^{\circ}$ taper on each side, $1.5{\pm}0.1mm$ shoulder margin, and 4mm crown height. The luting cements used in this study were as follow: 1. Uncemented 2. Zinc phosphate cements (Confi-Dental) 3. Conventional glass ionomer cement : Fuji 1 (GC) 4. Resin-modified glass ionomer cements : Fuji plus (GC) 5. Adhesive cements : Panavia F (Kuralay), Variolink II (Vivadent), Choice (Bisco). Fracture test using Instron. The crowns were loaded in compressive force to evaluate the effect of these cements on the breaking strength of these all-ceramic crowns. A steel ball with a diameter of 4mm was placed on the occlusal surface and load was applied to the steel ball by a cylindrical bolt with a crosshead speed of 0.5mm per minute until fracture occurred. The fractured surface was examined using Scanning Electron Microscopic Image (SEM) to discover the correlation between fracture strength and bonding capacity. Within the limitation of this in vitro study design, the results were as follows : 1. fomentations significantly increased the fracture resistance of Empress ceramic crowns compared to control. Uncemented (206.9 N): ZPC (812.9 N): Fuji 1 (879.5 N): Fuji Plus (937.7 N): Choice (1105.4 N): Variolink II (1221.1 N): Panavia F (1445.2 N). 2. Resin luting agent, treated by a silane bond enhancing agents, yielded a significant increase in fracture resistance. In some of the Panavia F group, a fracture extended into dentin. 3. According to SEM images of fractured Empress crowns, the stronger the bond at both interfaces(crown and die), the more fracture strength was acquired.

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Influence of Physico.Chemical Properties of Root Substrates on the Growth of Mother Plants and the Occurrence of Daughter Plants during the Propagation of 'Maehyang' Strawberry Using a Bag Culture System ('매향' 딸기 번식을 위한 플라스틱 백 재배시 상토 물리.화학성이 모주생육과 자묘 발생에 미치는 영향)

  • Choi, Jong-Myung;Park, Ji-Young;Ko, Kwan-Dal;Lee, Chi-Won W.
    • Korean Journal of Agricultural Science
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    • v.37 no.2
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    • pp.191-197
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    • 2010
  • The objective of this research was to determine the influence of the physical and chemical properties of root substrates used during the production of 'Maehyang' strawberry propaguleson the growth of the mother plants and the rate of daughter plant formation. Plants were cultured in plastic bags containing six different formulations of root substrates composed of: a) 50% coir dust and 50% perlite (5:5 by volume, A), b) 60% coir dust and 40% perlite (6:4, B), c) 70% coir dust and 30% perlite (7:3, C), d) 70% coir dust and 30% coconut chip (7:3 D), e) 60% coir dust and 40% coconut chip (60:40, E), or f) 50% sphagnum peat and 50% vermiculite (50:50, F). All media formulations contained a moderate level of base fertilizers. Physical and chemical properties of each formulation were determined before plant establishment and after 120 days of stock plant culture and runner production. Total porosity (TP) and container capacity (CC) of all substrate formulations were higher than 85% and 55%, respectively, allowing a suitable range of air and water holding characteristics. Formulation F provided the highest TP and CC values among the all substrate modifications evaluated. Substrate formulations A, B, C and F had higher electrical conductivity (EC) and $NO_3{^-}$-N concentrations than formulations D and E, when determined before and after plant culture. Formulations A, B, C, and F, having higher EC readings, also performed better as root substrates thanthe formulations D and E in increasing fresh and dry weights of the runners as well as the production of daughter plants per plant. The 'Maehyang' strawberry plants grown in the formulation F had the highest tissue N content, followed by those grown in substrate B, A, C, or D for 120 days after transplanting. Formulation F also facilitated accumulation of higher tissue phosphorus (P) and copper (Cu) contents compared to other treatments. Results of this experiment suggest that the chemical properties, rather than physical properties, of root substrates had a major influence on the growth of mother plants and the occurrence of healthy daughter plants during the bag-culture phase of propagation.

Characteristics of Aqueous Ammonia-CO2 reaction at Regeneration Condition of High Temperature and Pressure (고압고온 재생조건에서의 암모니아수-CO2 반응특성)

  • Kim, Yun Hee;Yi, Kwang Bok;Park, Sung Youl;Ko, Chang Hyun;Park, Jong-Ho;Beum, Hee Tae;Han, Myungwan;Kim, Jong-Nam
    • Korean Chemical Engineering Research
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    • v.48 no.2
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    • pp.253-258
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    • 2010
  • In the field of the $CO_2$ absorption process using aqueous ammonia, the effects of regeneration pressure and temperature on $CO_2$ absorption performances of the aqueous ammonia were investigated. The absorbents were prepared by dissolving ammonium carbonate solid in water to grant the resulted solution 0.5 $CO_2$ loading ($mol\;CO_2/mol\;NH_3$) and various ammonia concentration (14, 20, 26 and 32 wt%). As-prepared absorbents were regenerated at high pressure and temperature (over $120^{\circ}C$ and 6 bar) before the absorption test. The absorption test was carried out by injecting the simulated gas that contains 12 vol% of $CO_2$ into a bubbling reactor. The introduction of 26 wt% of the ammonia concentration for $CO_2$ absorption test resulted in the higher absorption capacities than other experimental conditions. In particular, when the absorbents with 26 wt% of the ammonia were regenerated at $150^{\circ}C$ and 14 bar, the highest absorption capacity, $45ml\;CO_2/g$, was obtained. According to the analysis of absorbents using acid-base titration, the ammonia loss during the regeneration of the absorbents with a fixed ammonia concentration decreased as the regeneration pressure increased, while it increased as the regeneration temperature increased. In the condition of fixed regeneration pressure and temperature, as expected, the ammonia loss increased as the ammonia concentration increased. The measured $CO_2$ loadings and ammonia concentrations of absorbents were compared to the values calculated by Electrolyte NRTL model in Aspen Plus.

Optimized Cell ID Codes for SSDT Power Control in W-CDMA System (W-CDMA 시스템의 최적의 SSDT 전력 제어용 셀 식별 부호)

  • Young-Joon Song;Bong-Hoe Kim;Hae Chung
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.13 no.8
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    • pp.804-810
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    • 2002
  • The code division multiple access(CDMA) system capacity is limited by the amount of interference of the system. To reduce the unnecessary interference, this paper proposes optimized cell identification codes for site selection diversity transmission(SSDT) power control in wideband code division multiple access system of third generation partnership project(3GPP). The main objective of SSDT power control is to transmit on the downlink from the primary cell, and thus reducing the interference caused by the multiple transmission. In order to select a primary cell, each cell is assigned a temporary identification(ID) and user equipment(UE) periodically informs a primary cell ID to the connecting cells during soft handover. The non-primary cells selected by UE do not transmit the dedicated physical data channel(DPDCH) to reduce the interference. A major issue with the SSDT technology is the impact of uplink symbol errors on its performance. These errors can corrupt the primary ID code and this may lead to wrong decoding in the base station receivers. The proposed SSDT cell ID codes are designed to minimize the problem and to be easily decoded using simple fast Hadamard transformation(FHT) decoder.

The Spatial Characteristics of Network in Zhongguancun Cluster - Focus on the Corporate Activities - (중관촌(中關村) 클러스터 네트워크의 공간적 특성 - 기업 활동을 중심으로 -)

  • Zhan, Jun
    • Journal of the Korean association of regional geographers
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    • v.18 no.3
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    • pp.298-309
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    • 2012
  • This paper studies the characteristics of the network of the Zhongguancun Cluster, the most representative innovative cluster of the high-tech industry in China at present. For this study, Zhongguancun Cluster was the first high-tech cluster created in China in 1988, the current Zhongguancun Cluster plays a leading role in the development of the high-tech industry in China. In addition, the Zhongguancun Cluster has attracted global attention and helped elevate China as a key region in terms of research development in relation to the high-tech industry. With regard to the spatial characteristics of the network belonging to the companies in Zhongguancun Cluster, purchase and producer services and information and R&D network have a strong tendency to be local, while on the other hand the product sales network has a strong tendency to be non-local. It is because the political support supplied by the government, institutional base that provides high-tech companies, producer services and information regarding producer services is relatively well prepared and managed in Zhongguancun Cluster. The spatial characteristics of the R&D network have a very strong local character is due to the location of the Zhongguancun Cluster where companies, universities and research centers with outstanding research development capacity as well as various support organizations for technology innovation within the cluster are included. On the other hand, because the high-tech products produced in this area are sold all across China as well as in foreign countries, the product sales network has a strong non-local character. Strengthening the local network in terms of the main agents of the cluster is the most important aspect in order to develop a certain industrial cluster into an innovative cluster. In this respect, if the Zhongguancun Cluster is seen from the perspective of a network, it has a basic network foundation. However, to strengthen international competitiveness, not only the local network but also the international network should be strengthened.

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Remediation of Petroleum-Contaminated Soil by a Directly-Heated Thermal Desorption Process (직접 가열식 열탈착 공정을 이용한 유류오염토양의 정화)

  • Min, Hyeong-Sik;Yang, In-Ho;Jeon, Sang-Jo;Kim, Han-S.
    • Journal of Soil and Groundwater Environment
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    • v.14 no.5
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    • pp.62-70
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    • 2009
  • A field soil highly contaminated with petroleum hydrocarbons (JP-8 and diesel fuels) was employed for its remediation by a lab-scale thermal desorption process. The soil was collected in the vicinity of an underground storage tank in a closed military base and its contamination level was as high as 4,476 ppm as total petroleum hydrocarbon (TPH). A lab scale directly-heated low temperature thermal desorption (LTTD) system of 10-L capacity was developed and operated for the thermal treatment of TPH contaminated soils in this study. The desired operation temperature was found to be approximately $200-300^{\circ}C$ from the thermal gravimetric analysis of the contaminated field soils. The removal efficiencies higher than 90% were achieved by the LTTD treatment at $200^{\circ}C$ for 10 min as well as at $300^{\circ}C$ for 5 min. As the water content in the soils increased and therefore they were likely to be present as lumps, the removal efficiency noticeably decreased, indicating that a pre-treatment such as field drying should be required. The analysis of physical and chemical properties of soils before and after the LTTD treatment demonstrated that no significant changes occurred during the thermal treatment, supporting no needs for additional post-treatments for the soils treated by LTTD. The results presented in this study are expected to provide useful information for the field application and verification of LTTD for the highly contaminated geo-environment.

The Morphology and Physical and Chemical Characteristics of the Changpyeong Series Derived from Old Alluvium (홍적층(洪積層)에 기인(基因)된 적황색토(赤黃色土)의 형태(形態) 및 물리적(物理的) 화학적(化學的) 특성(特性)에 관(關)한 연구(硏究) - 창평통(昌平統)에 관(關)하여 -)

  • Shin, Y.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.3 no.1
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    • pp.61-66
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    • 1970
  • This study examined the morphology and physical and chemical characteristics of the Changpyeong series developed on gently sloping to rolling relief on dissected old paddiplains and terraces. This soil has dark brown silty clay loam A horizons, very thick dark red to red silty clay or clay Bt horizons, and C horizons of old alluvial materials frequently with strongly weathered round cobbles and pebbles. It is strongly acid with a low organic matter content, relatively low in cation exchange capacity, but with relatively high base-status based on amount of extractable cations. There is no obvious changes in particle size distribution with depth and textural B horizons probably have not been formed by podzolization but formed by mechanical movement of clay. This soil is classified as Typic Hapludalfs in USDA 7th approximation and Brunic Luvisols in FAO classification system.

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Chemical Characteristics of soils in Cheju Island -II. Variations in Chemical Characteristics of the Citrus Orchard Soils as a Function of Years of Cultivation (제주도(濟州道) 토양(土壤)의 화학적(化學的) 특성(特性) 조사(調査) 연구(硏究) -II. 경작년대(耕作年代)에 따른 감귤원(柑橘園) 토양(土壤)의 화학적(化學的) 특성(特性) 변화(變化))

  • Yoo, Sun-Ho;Song, Kwan-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.2
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    • pp.161-166
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    • 1984
  • The Cheju citrus orchards have been chemically fertilized with both phosphates high in Ca and Mg contents and potassium. The amount of chemical fertilizers consumed per unit area of cultivated land and the ratios of $P_2O_5$ and $K_2O$ to N in chemical fertilizers applied were much higher in the Cheju Island than those of the Korean mainland. These trends were particularly prominant in Southern Cheju where 70% of the Cheju citrus orchards are located. As the result of heavy applications of phosphate fertilizers, the available phosphorus of citrus orchard soils increased with increasing number of years under cultivation. The available phosphorus of sub-soil increased slightly with the number of years under cultivation compared to that of surface soil. Base saturation and pH increased with the number of years under cultivation, inasmuch as exchangeable bases such as Ca, Mg and K increased predominantly, but the cation exchange capacity did not vary in the citrus orchard soils.

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A Study on the Development Lightweight Aggregate using Clink Ash for Reduction in Freezing Ground (지반의 동결저감 대책기술을 수립하기 위한 클링커애쉬 인공경량골재 개발에 관한 연구)

  • Moon, Jong-Wook
    • Fire Science and Engineering
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    • v.23 no.6
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    • pp.116-125
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    • 2009
  • This study is progressed function ratio, it's trued taste by an experiment to present data for human work light weight aggregate development that use clink ash progressed liquid limit, small success limit, wear loss in quantity, sand equivalent, sieve cutting examination. 80 : 20's match of function rain examination is 1.4, and that use rubble Goljae as ckink ash lightweight aggregate's capacity ratio increases by 1.0 increase of function rain many. Also, examination multiplied delicate flavor gradually according to increase of the mixing rate, and absorption coefficient increased. This is judged by phenomenon that appear by special quality upper clink ash of polystyrene bid and porosity's increase between lightweight aggregate. It is case that use aggregate of wear loss in quantity is 13.5 in sand equivalent and a wear loss in quantity experiment and although case that mix 20% increases by 14.4, this phenomenon by weak tissue of lightweight aggergate be judge. When it's as a these experiment, the statue prevention floor of a street improvement specifications is prescribing so that satisfy by sand equivalent 20, CBR 10. This is showed result that this satisfies in quality standard all in match experiment ago that see.