• 한국초지조사료학회지
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• 제19권3호
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• pp.273-280
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• 1999

알팔파의 하배축(hypocotyl)으로부터 캘러스 유도 및 식물체 재분화를 위하여 2.4-D 와 kinetin이 조합 처리된 MS 배지에 조직을 치상하였을 때 4주 후 캘러스가 유도되었으며, 2.4-D $4mg/{\ell}$와 kinetin $0.1mg/{\ell}$ 그리고 2.4-D $4mg/{\ell}$와 kinetin $0.5mg/{\ell}$ 조합에서 체세포배가 형성되었다. 성숙한 체세포배를 MS 기본배지로 계대배양하였을 때 정상적인 식물체로 재분화 하였다. 이차 체세포배 발생을 위하여 재분화된 기내식물의 자엽으로부 터 이차 캘러스를 유도하였다. 2.4-D의 농도에 따라 배발생 캘러스의 형성률에 차이를 보였으며, 2.4-D $4mg/{\ell}$의 MS 배지에서 배양하였을 때 배발생 캘러스의 유도가 가장 좋았다. 배발생 캘러스로부터 이차 체세포배의 발생률을 2.4-D $0.1mg/{\ell}$ 첨가한 MS 배지에서 가장 좋았으며, 캘러스당 배 발생률이 일차 캘러스 보다 평균 18배 증가하여, 이차 체세포배 배양에 의한 재분화 식물체의 대량증식이 가능하였다. 성숙한 이차 체세포배는 MS 기본배지에 계대배양 하였을 때 뿌리가 유도되었으며 정상적인 식물체로 발달하였다.

• Journal of Microbiology and Biotechnology
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• 제18권12호
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• pp.1903-1907
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• 2008

Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin production by Hahella chejuensis KCTC 2396, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among 12 components included in basal medium, $NaHCO_3$, ${Na}_{2}{SiO}_{3}$, ${NH_4}{NO_3}$, ${Na}_{2}{SO}_{4}$ and $CaCl_2$ were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: 1% sucrose; 0.4% peptone; 0.1 % yeast extract; and (g/l): NaCl, 20.0; ${Na}_{2}{SO}_{4}$, 9.0; $CaCl_2$, 1.71; KCl, 0.4; and (mg/l): ${H_3}{BO_3}$, 10.0; KBr, 50.0; NaF, 2.0; $NaHCO_3$, 45.0; ${Na}_{2}{SiO}_{3}$, 4.5; ${NH_4}{NO_3}$, 4.5. The predicted maximum yield of prodigiosin in the optimized medium was 1.198 g/l by the Box-Behnken design, whereas the practical production was 1.495 g/l, which was three times higher than the basal medium (0.492 g/l).

• 생명과학회지
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• 제11권4호
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• pp.340-345
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• 2001

Effects of growth regulators(2,4-D, IAA, NAA, BA and kinetin) and chilling treatment on callus induction. embryogenesis and regeneration through anther cultures of B. falcatum L. were examined. Frequency of callus induction and embryogenesis was effectively increased by the treatment of chilling at 5$^{\circ}C$ for 10 days before anther inoculation. Optimal level of growth regulator for callus induction and embryogenesis from anther was 2,4-D 1.0 mg/L in Murashige and Skoog(MS) basal medium supplemented with 30 g/L sucrose, 8 g/L agar. Frequency of embryogenesis from anther derived callus was increased up to 48% or 45% by addition of IAA 0.1+ kinetin 1.0 mg/L of IAA 0.1+ BA 1.0 mg/L in MS medium, respectively, Optimal medium for obtaining green callus was MS basal supplemented with BA 1.0mg/L. Addition of auxins(IAA or NAA) inhibited the formation of green callus from anther derived callus.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.218-225
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• 2007

A lactic acid bacterium capable of anaerobic respiration was isolated from soil with ferric iron-containing glucose basal medium and identified as L. garvieae by using 16S rDNA sequence homology. The isolate reduced ferric iron, nitrate, and fumarate to ferrous iron, nitrite, and succinate, respectively, under anaerobic $N_2$ atmosphere. Growth of the isolate was increased about 30-39% in glucose basal medium containing nitrate and fumarate, but not in the medium containing ferric iron. Specifically, metabolic reduction of nitrate and fumarate is thought to be controlled by the specific genes fnr, encoding FNR-like protein, and nir, regulating fumarate-nitrate reductase. Reduction activity of ferric iron by the isolate was estimated physiologically, enzymologically, and electrochemically. The results obtained led us to propose that the isolate metabolized nitrate and fumarate as an electron acceptor and has specific enzymes capable of reducing ferric iron in coupling with anaerobic respiration.

• 한국수정란이식학회지
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• 제7권2호
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• pp.125-131
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• 1992

The nudear changes of bovine oocytes during 24 hrs. of culture for mejotic maturation were examined. Bovine oocytes were collected from small(<2 mm), medium(2~6 mm) and large(>6mm) follicles and classified into three grades by their morphological characteristics. A total of 242 oocytes collected were obtained:from 184 small, 157 medium and 1 large follicles, respectively and were classified into 95 grade I, 155 grade H and 92 grade III oocytes. All the bovine oocytes collected and graded were washed with a basal medium and incubated in groups of 10 for 24 hrs in 5% $CO_2$ and 39$^{\circ}C$. The basal medium used was composed of TCM-199 supplemented with sodium bicarbonate, sodium pyruvate, streptomycin, penicillin G and 10% FCS. The oocytes were cultured in drops of 50,$\mu$l basal medium supplemented with 35$\mu$g /ml FSH, 10$\mu$g /ml LH and 1$\mu$g /ml estradiol-17$\beta$. The oocytes were fixed and examined on their chromosomal status by 1% acetorcein staining in the interval of 3 hrs. Most of the grade I oocytes developed to germinal vesicule stage at 0 to 3 hrs., germinal vesicle breakdown at 6 hrs., metaphase I at 9 to 15 hrs., anaphase I and telophase I at 18 hrs., and metaphase II and the first polar body at 24 hrs. after culture for meiotic maturation. However, it was found that compared to grade I oocytes, grade H and W oocytes reached earlier to germinal vesicle breakdown and most of them developed earlier to M II stage at 21 hrs. after culture.

• 한국미생물·생명공학회지
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• 제10권2호
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• pp.101-108
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• 1982

인삼extract와 인삼saponin의 세균 세포 증식에 대한 영향은 세균의 종 및 인삼 성분농도에 따라 차이가 있었다. Serratia marcescens, Aevobacter aerogenes등은 인삼extract 0.1%첨가 농도에서 최대 증식을 나타내었으나 1%, 농도 이상에서는 증식이 억제되었으며 Staphylo. coccus aureus, Escherichia coli등은 1% 농도에서 최대 증식을 나타내었으나 5% 농도에서는 증식이 억제되었다. 인삼 saponin 0.002 %, 농도에서는 Micrococcus flavus, Aerobacter aerogenes등은 증식이 약간 촉진되나 0.02%, 이상의 농도에서는 증식이 현저히 억제되고 Serratia속 세균에서는 인삼saponin이 증식 억제 인자로 작용하였으나 Erwinia aroideae, Sarcina margtnata등에서는 세균 증식에 무관한 것으로 나타났다. 이러한 인삼 saponin이 나타내는 항균활성의 원인 물질은 ginsenoside Rg$_1$이었다.

• 한국산림과학회지
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• 제84권2호
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• pp.145-150
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• 1995

기내배양된 아까시나무(Robinia pseudoacacia L.)의 줄기기부에서 발생된 callus로부터 기관발생을 통하여 식물체재분화 시스템을 확립하였다. Callus는 줄기를 BA 또는 NAA가 함유된 MS배지에 배양하였을 때 유도되었으며, BA처리구가 NAA처리구보다 유도율이 높았다. BA가 첨가된 배지에 줄기를 배양하였을 때 기부 callus의 생장은 광조건 및 암조건에서도 잘 증식되었다. 줄기기부에서 발생된 callus는 녹색과 희면서 노란색을 띠는 callus로 분리되어 2.0mg/l BA와 0.5mg/l NAA가 함유된 mMS배지에 배양하였을 때 녹색의 callus로부터 줄기가 재분화되었다. 재분화된 줄기는 생장조절제가 함유되지 않은 ${\frac{1}{2}}MS$배지에서 발근되었다.

• 한국자원식물학회지
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• 제35권4호
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• pp.508-514
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• 2022

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 mg/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 mg/L BAP and 1.0 mg/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

• Current Research on Agriculture and Life Sciences
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• 제6권
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• pp.75-85
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• 1988

포푸라(양황철)의 기내배양(器內培養)에서 대량증식(大量增殖)한 유전적(遺傳的)으로 균일(均一)한 시료(試料)를 이용(利用), NaCl내성(耐性)을 검정(檢定)한 결과(結果) 50mM이 생육한계(生育限界)임을 밝혀냈다. 엽표피(葉表皮)를 침(針)으로 뚫어 MS 기본배지(基本培地)에 Auxin과 Cytokinin의 효과(效果)를 검정(檢定)한 결과(結果) Cytokinin에서는 많은 개체(個體)가 발생(發生)하였으며 특(特)히 BAP $0.8mg/{\ell}$에서는 127.6개의 많은 개체(個體)가 발생(發生)하였다. NaCl 내성개체(耐性個體) 선발(選拔)을 위(爲)해 같은 배지(培地)에 NaCl 10mM부터 100mM까지 10단계로 처리(處理)하여 침(針)으로 자극한 시료(試料)를 접종(接種)한 결과(結果) 50mM 이상(以上)에서 13.7~15.7개의 여러가지 개체(個體)를 얻었다. 특(特)히 90mM에서 10.7개 100mM에서도 8.3개의 개체(個體)를 얻었다. 비선발(非選拔) 개체(個體)는 50mM 이상(以上)의 NaCl 첨가(添加)배지에서 생장(生長)이 완전(完全)히 중지(中止)된 반면(反面)에 기내선발개체(器內選拔個體)는 고농도(高濃度) NaCl의 계대배양에서도 생장(生長)를 계속하였다. 이상(以上)의 결과(結果)에서 임목(林木)에 있어서도 기내배양법(器內培養法)를 이용(利用)하여 내성(耐性) 개체(個體)를 선발(選拔)할 수 있는 가능성(可能性)을 추정(推定)할 수 있었다.

• Journal of Plant Biotechnology
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• 제50권
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• pp.183-189
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• 2023

This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.