• 비파괴검사학회지
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• 제33권6호
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• pp.511-518
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• 2013

Steam Generator (SG) tube, as a barrier isolating the primary coolant system from the secondary side of nuclear power plants (NPP), must maintain the structural integrity for the public safety and their efficient power generation. So, SG tubes are subject to the periodic examination and the repairs if needed so that any defective tubes are not in service. Recently, corrosion related degradations were detected in the tubes of the domestic OPR-1000 NPP, as a form of axially oriented outer diameter stress corrosion cracking (ODSCC). According to the studies on the factors causing the heat fouling as well as developing corrosion cracking, densely scaled deposits on the secondary side of the SG tubes are mainly known to be problematic causing the adverse impacts against the soundness of the SG tubes [1]. Therefore, the processes of various cleaning methods efficiently to dissolve and remove the deposits have been applied as well as it is imperative to maintain the structural integrity of the tubes after exposing to the cleaning agent. So qualification test (QT) should be carried out to assess the perfection of the chemical cleaning and QT is to apply the processes and to do ECT. In this paper, the chemical cleaning processes to dissolve and remove the scaled deposits are introduced and results of ECT on the artificial crack specimens to determine the effectiveness of those processes are represented.

• Journal of Korean Neurosurgical Society
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• 제58권5호
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• pp.494-498
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• 2015

Objective : To describe a novel spinous process-splitting hemilaminoplasty technique for the surgical treatment of intradural and posterior epidural lesions that promotes physiological restoration. Methods : The spinous process was split, the area of the facet lamina junction was drilled, and en bloc hemilaminectomy was then performed. After removing intradural and posterior epidural lesions, we fitted the previously en bloc-removed bone to the pre-surgery same shape, and held it in place with non-absorbable sutures. Surgery was performed on 16 laminas from a total of nine patients between 2011 and 2014. Bony union of the reconstructed lamina was assessed using computed tomography (CT) at 6 months after surgery. Results : Spinous process-slitting hemilaminoplasty was performed for intradural extramedullary tumors in eight patients and for ossification of the ligament flavum in one patient. Because we were able to visualize the margin of the ipsilateral and contralateral dura, we were able to secure space for removal of the lesion and closure of the dura. None of the cases showed spinal deformity or other complications. Bone fusion and maintenance of the spinal canal were found to be perfect on CT scans. Conclusion : The spinous process-splitting hemilaminoplasty technique presented here was successful in creating sufficient space to remove intradural and posterior epidural lesions and to close the dura. Furthermore, we were able to maintain the physiological barrier and integrity after surgery because the posterior musculature and bone structures were restored.

• BMB Reports
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• 제49권4호
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• pp.214-219
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• 2016

A nucleosomal protein, high mobility group box 1 (HMGB1) is known to be a late mediator of sepsis. Dabrafenib is a B-Raf inhibitor and initially used for the treatment of metastatic melanoma therapy. Inhibition of HMGB1 and renewal of vascular integrity is appearing as an engaging therapeutic strategy in the administration of severe sepsis or septic shock. Here, we examined the effects of dabrafenib (DAB) on the modulation of HMGB1-mediated septic responses. DAB inhibited the release of HMGB1 and downregulated HMGB1-dependent inflammatory responses by enhancing the expressions of cell adhesion molecules (CAMs) in human endothelial cells. In addition, treatment with DAB inhibited the HMGB1 secretion by CLP and sepsis-related mortality and pulmonary injury. This study demonstrated that DAB could be alternative therapeutic options for sepsis or septic shock via the inhibition of the HMGB1 signaling pathway.

• 한국임상수의학회지
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• 제30권4호
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• pp.283-287
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• 2013

Canine skin is a highly dynamic organ that is constantly adapted to changes in its environment. It provides structural, sensory, immunologic, and physiologic functions and contributes an essential barrier function against potential environmental insults. We assessed the changes of canine skin hydration, pH, and protein contents by noninvasive method according to the frequency of application with canine commercial shampoo. Canine commercial shampoo was applied topically every other day, 5 days, and 8 days on different sites, respectively in 8 dogs. Saline-applied site was as a control. Skin hydration was increased and skin surface pH was decreased significantly in canine commercial shampoo-applied site every other day (p < 0.05). Also, skin protein concentration measured by D-squame$^{(R)}$ tape stripping method was increased significantly in canine commercial shampoo-applied site every other day (p < 0.05). There were alterations on every 5 days and 8 days, but not significantly. These results may be recognized that the frequent use of canine commercial shampoo cause abnormalities of the skin barrier function and alteration of stratum corneum integrity/cohesion. Consequently it was suggested that bathing the dog with canine commercial shampoo was appropriate every 5 or 8 days.

• 방사성폐기물학회지
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• 제15권3호
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• pp.219-230
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• 2017

본 연구는 경주 중 저준위처분장 2단계 표층처분시설의 폐쇄 후 안전성에 대한 불확실성을 예측 평가하기 위하여 수행되었다. 다중덮개와 처분고의 건전/열화를 고려한 총4가지의 시나리오를 도출하여 강우침투 시 예상되는 처분시설 내부의 유체 이동을 모사하였다. 강우 조건은 총 30년(1985~2014) 간의 월평균 데이터를 적용하였으며, 시뮬레이션 기간은 제도적 관리기간인 300년으로 설정하였다. 처분덮개와 처분고 콘크리트 모두 건전성을 유지하는 조건의 기본 시나리오 평가 결과, 처분시설 내부의 처분고를 완전히 포화시키지 못하는 것을 확인할 수 있었다. 다중 덮개층을 구성하는 8개 층의 각 매질의 모세관 압력과 투과도 차이로 인하여 다중 덮개층이 효과적으로 차수 배수 역할을 하는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.863-876
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• 2019

Farm animals such as piglets are often affected by environmental stress, which can disturb the gut ecosystem. Antibiotics were commonly used to prevent diarrhea in weaned piglets, but this was banned by the European Union due to the development of antibiotic resistance. However, the use of probiotics instead of antibiotics may reduce the risk posed by pathogenic microorganisms and reduce the incidence of gastrointestinal diseases. Therefore, this study was conducted to investigate the effects of Lactobacillus casei Zhang on the mechanical barrier and immune function of early-weaned piglets infected using Escherichia coli K88 based on histomorphology and immunology. Fourteen-day-old weaned piglets were divided into a control group and experimental groups that were fed L. casei Zhang and infected with E. coli K88 with or without prefeeding and/or postfeeding of L. casei Zhang. The L. casei Zhang dose used was $10^7CFU/g$ diet. Jejunum segments were obtained before histological, immunohistochemical, and western blot analyses were performed. In addition, the relative mRNA expression of toll receptors and cytokines was measured. Piglets fed L. casei Zhang showed significantly increased jejunum villus height, villus height-crypt depth ratio, muscle thickness, and expression of proliferating cell nuclear antigen and tight junction proteins ZO-1 and occludin. The use of L. casei Zhang effectively reduced intestinal inflammation after infection. We found that L. casei Zhang feeding prevented the jejunum damage induced by E. coli K88, suggesting that it may be a potential alternative to antibiotics for preventing diarrhea in early-weaned piglets.

• 한국동물생명공학회지
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• 제39권2호
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• pp.105-113
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• 2024

Background: Aflatoxin B1 (AFB1) is a toxic metabolite generated by Aspergillus species and is commonly detected during the processing and storage of food; it is considered a group I carcinogen. The hepatotoxic effects, diseases, and mechanisms induced by AFB1 owing to chronic or acute exposure are well documented; however, there is a lack of research on its effects on the intestine, which is a crucial organ in the digestive process. Dogs are often susceptible to chronic AFB1 exposure owing to lack of variation in their diet, unlike humans, thereby rendering them prone to its effects. Therefore, we investigated the effects of AFB1 on canine small intestinal epithelial primary cells (CSIc). Methods: We treated CSIc with various concentrations of AFB1 (0, 1.25, 2.5, 5, 10, 20, 40, and 80 μM) for 24 h and analyzed cell viability and transepithelial-transendothelial electrical resistance (TEER) value. Additionally, we analyzed the mRNA expression of tight junction-related genes (OCLN, CLDN3, TJP1, and MUC2), antioxidant-related genes (CAT and GPX1), and apoptosis-related genes (BCL2, Bax, and TP53). Results: We found a significant decrease in CSIc viability and TEER values after treatment with AFB1 at concentrations of 20 μM or higher. Quantitative polymerase chain reaction analysis indicated a downregulation of OCLN, CLDN3, and TJP1 in CSIc treated with 20 μM or higher concentrations of AFB1. Additionally, AFB1 treatment downregulated CAT, GPX1, and BCL2. Conclusions: Acute exposure of CSIc to AFB1 induces toxicity, and exposure to AFB1 above a certain threshold compromises the barrier integrity of CSIc.

• 한국산림과학회지
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• 제101권3호
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• pp.412-425
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• 2012

산지계곡부의 토석류 유출을 저지하는 콘크리트 사방댐은 콘크리트의 재료 및 시공 불량, 콘크리트의 열화로 인한 내구성 저하, 국부적으로 발생한 균열 등의 이유로 인하여 사방댐의 일부 또는 전체 구조물이 붕괴되어 그 기능을 손실하는 경우가 있다. 본 논문에서는 이러한 콘크리트 사방댐의 구조적 문제점과 안전성을 파악하여 사전에 대책을 강구하고 효율적인 유지관리를 할 수 있는 방안을 제안하였다. 그 방안은 콘크리트 사방댐 구조물의 콘크리트 압축강도를 비파괴적으로 평가하여 품질등급을 평가하는 것으로, 등급평가 단계에서 비파괴 콘크리트 압축강도 평가를 위해 SASW 기법이라고 하는 표면파 기법을 도입하였다. 또한 SASW 기법을 콘크리트 사방댐의 안정성 가에 활용할 수 있도록 SASW 시험의 현장적용방안, 콘크리트 결함에 따른 자료분석 기법, 평가기준에 대한 제안등에 대한 연구를 수행하였다. 제안된 기법의 적용성과 신뢰성을 평가하기 위하여, 20년 이상 경과된 노후 콘크리트 방댐 5곳에 제안된 표면파 기법의 적용방안을 적용하여 보았다. 그 결과 콘크리트 사방댐의 여러 가지 콘크리트 함에 대해서도 SASW 기법에 의한 품질평가는 신뢰성 있게 수행될 수 있음을 확인할 수 있었고, 본 연구에서 제 안한 품질등급 평가 결과는 사방협회에서 실시한 사방댐 점검결과와 일치하는 결과를 제시하였다.

• 한국전산구조공학회논문집
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• 제28권5호
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• pp.535-542
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• 2015

원자로 격납건물은 냉각재상실사고와 같이 내부의 과도한 압력이 유발되는 사고에 있어서도 방사성 물질이 외부로 누출되지 않도록 막는 최종의 방벽이다. 이러한 격납건물의 기능적 중요성에 기인하여, 건설 초기 구조건전성시험(SIT)을 수행한다. 이러한 SIT거동을 가장 실제와 가깝게 예측하기 위한 해석 연구를 수행하였다. 해당 연구의 결과는 2편의 논문으로 정리되었는데, 본 논문은 그 중 II편으로 I편의 해석모델 구성 시의 주요 고려사항의 분석 및 예비해석 결과를 반영한 상세 해석 모델의 구성 과정 및 해석 결과를 제시하고 있다. 특히 비부착식 텐던으로 시공된 구조물에서 덕트관에 의한 강성 저감효과 및 덕트관을 사이에 둔 텐던과 콘크리트간의 밀착 여부에 따른 영향을 해석 시 최대한 고려하고자 하였다. 이러한 과정을 통해 구축된 해석 모델에 따른 변위과 신고리 3호기 SIT 측정변위를 비교한 결과, ASME CC-6000 기준을 충분히 만족시키는 결과가 나타남을 확인하였다.

• 한국전산구조공학회논문집
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• 제28권5호
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• pp.523-533
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• 2015

원자로 격납건물은 냉각재상실사고와 같이 내부의 과도한 압력이 유발되는 사고에 있어서도 방사성 물질이 외부로 누출되지 않도록 막는 최종의 방벽이다. 이러한 격납건물의 기능적 중요성에 기인하여, 건설 초기 구조건전성시험(SIT)을 수행한다. 신고리 3호기 SIT 시험 당시 계측된 변위를 예측하기 위한 초기 해석 모델은 일부 위치에서 실제 변위를 과소 평가하는 경향을 보임에 따라 이를 개선하고자 하는 연구가 수행되었다. 해당 연구의 결과를 I 편과 II 편의 논문으로 정리하였으며, 본 I 편에서는 초기 해석모델을 개선해가는 과정에서의 해석모델 구성 시의 주요 고려사항의 분석 및 예비해석 결과를 제시하고 있다. 우선적으로 콘크리트 자체의 해석요소(mesh) 구성과 라이너, 철근, 텐던 등의 요소간의 연결 설정이 중요함을 확인하였다. 또한, 다양한 예비해석의 결과를 통해 비부착식 텐던으로 시공된 구조물에서 덕트관에 의한 강성 저감 효과 및 덕트관을 사이에 둔 텐던과 콘크리트간의 밀착 여부에 따른 강성 영향을 적절히 고려하는 것이 중요함을 확인하였다.