• Title/Summary/Keyword: Barrel Alignment

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Local structural alignment and classification of TIM barrel domains

  • Keum, Chang-Won;Kim, Ji-Hong;Jung, Jong-Sun
    • Bioinformatics and Biosystems
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    • v.1 no.2
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    • pp.123-127
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    • 2006
  • TIM barrel domain is widely studied since it is one of most common structure and mediates diverse function maintaining overall structure. TIM barrel domain's function is determined by local structural environment at the C-terminal end of barrel structure. We classified TIM barrel domains by local structural alignment tool, LSHEBA, to understand characteristics of TIM barrel domain's functionalvariation. TIM barrel domains classified as the same cluster share common structure, function and ligands. Over 80% of TIM barrels in clusters share exactly the same catalytic function. Comparing clustering result with that of SCOP, we found that it's important to know local structural environment of TIM barrel domains rather than overallstructure to understand specific structural detail of TIM barrel function. Non TIM barrel domains were associated to make different domain combination to form a different function. The relationship between domain combination, we suggested expected evolutional history. We finally analyzed the characteristics of amino acids around ligand interface.

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Study on Analyzing and Correction of Dynamic Battery Alignment Error in Naval Gun Fire Control System by using Image of Boresight Telescope (포배열카메라 영상을 활용한 함포 사격통제시스템의 동적배열오차 분석 및 보정방법)

  • Kim, Eui-Jin;Suh, Tae Il
    • Journal of the Korea Institute of Military Science and Technology
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    • v.16 no.6
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    • pp.745-751
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    • 2013
  • In naval gun firing, firing accuracy comes from the combination of each component's accuracy in CFCS (Command and Fire Control System) like tracking sensors and gun. Generally, battery alignment is done to correct the error between gun and tracking sensor by using boresight telescope on harbor and sea. But normally, the battery alignment can compensate only the static alignment error and ignore dynamic alignment error which is caused by own ship movement. There was no research on this dynamic alignment error until now. We propose a new way to analyze dynamic arrangement error by using image of boresight telescope. In case of the dynamic alignment error was due to time delay of own ship attitude information, we propose the way to compensate it.

Opto - Mechanical Design of IGRINS Slit-viewing Camera Barrel

  • Oh, Hee-Young;Yuk, In-Soo;Park, Chan;Lee, Han-Shin;Lee, Sung-Ho;Chun, Moo-Young;Jaffe, Daniel T.
    • Bulletin of the Korean Space Science Society
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    • 2011.04a
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    • pp.31.2-31.2
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    • 2011
  • IGRINS (Immersion GRating INfrared Spectrometer) is a high resolution wide-band infrared spectrograph developed by Korea Astronomy and Space Science Institute (KASI) and the University of Texas at Austin (UT). The slit-viewing camera is one of four re-imaging optics in IGRINS including the input relay optics and the H- and K- band spectrograph cameras. Consisting of five lenses and one Ks-band filter, the slit viewing camera relays the infrared image of $2'{\times}2'$ field around the slit to the detector focal plane. Since IGRINS is a cryogenic instrument, the lens barrel is designed to be optimized at the operating temperature of 130 K. The barrel design also aims to achieve easy alignment and assembly. We use radial springs and axial springs to support lenses and lens spacers against the gravity and thermal contraction. Total weight of the lens barrel is estimated to be 1.2 kg. Results from structural analysis are presented.

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Alteration of Spindle Formation and Chromosome Alignment in Post-Ovulatory Aging of Mouse Oocytes

  • Kang, Hee-Gyoo;Cha, Byung-Hun;Jun, Jin-Hyun
    • Development and Reproduction
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    • v.15 no.3
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    • pp.231-237
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    • 2011
  • The objective of this study was to elucidate the dynamics of microtubules in post-ovulatory aging in vivo and in vitro of mouse oocytes. The fresh ovulated oocytes were obtained from oviducts of superovulated female ICR mice at 16 hours after hCG injection. The post-ovulatory aged oocytes were collected at 24 and 48 hours after hCG injection from in vivo and in vitro, respectively. Immunocytochemistry was performed on ${\beta}$-tubulin and acetylated ${\alpha}$-tubulin. The microtubules were localized in the spindle assembly, which was barrel-shaped or slightly pointed at its poles and located peripherally in the fresh ovulated oocytes. The frequency of misaligned metaphase chromosomes were significantly increased in post-ovulatory aged oocytes after 48 hours of hCG injection. The spindle length and width of post-ovulatory aged oocytes were significantly different from those of fresh ovulated oocytes, respectively. The staining intensity of acetylated ${\alpha}$-tubulin showed stronger in post-ovulatory aged oocytes than that in the fresh ovulated oocytes. In the aged oocytes, the spindles had moved towards the center of the oocytes from their original peripheral position and elongated, compared with the fresh ovulated oocytes. Microtubule organizing centers were formed and observed in the cytoplasm of the aged oocytes. On the contrary, it was not observed in the fresh ovulated oocytes. The alteration of spindle formation and chromosomes alignment substantiates the poor development and the increase of disorders from the post-ovulatory aged oocytes. It might be important to fertilize on time in ovulated oocytes for the developmental competence of embryos with normal karyotypes.

Automatic Focus Control for Assembly Alignment in a Lens Module Process (렌즈 모듈 생산 공정에서 조립 정렬을 위한 자동 초점 제어)

  • Kim, Hyung-Tae;Kang, Sung-Bok;Kang, Heui-Seok;Cho, Young-Joon;Park, Nam-Gue;Kim, Jin-Oh
    • Journal of the Korean Society for Precision Engineering
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    • v.27 no.2
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    • pp.70-77
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    • 2010
  • This study proposed an auto focusing method for a multi-focus image in assembling lens modules in digital camera phones. A camera module in a camera phone is composed of a lens barrel, an IR glass, a lens mount, a PCB board and aspheric lenses. Alignment among the components is one of the important factors in product quality. Auto-focus is essential to adjust image quality of an IR glass in a lens holder, but there are two focal points in the captured image due to thickness of IR glass. So, sharpness, probability and a scale factor are defined to find desired focus from a multi-focus image. The sharpness is defined as clarity of an image. Probability and a scale factors are calculated using pattern matching with a registered image. The presented algorithm was applied to a lens assembly machine which has 5 axes, two vacuum chucks and an inspection system. The desired focus can be determined on the local maximum of the sharpness, the probability and the scale factor in the experiment.

Ultrasonic Inspection of RPV Internal Structures (원자로 내부 구조물 초음파검사 현황)

  • Sim, C.M.;Choi, H.L.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.16 no.1
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    • pp.46-51
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    • 1996
  • 원자로는 압력용기 및 내부 구조물로 구분되어 있다. 내부 구조물들의 경련 열화 현상에 따라 결함이 많이 발생하여 이에 대한 초음파검사가 요구되고 있다. 따라서 원자로 내부 구조물에 대한 초음파검사 현황 및 각각 구조물들의 검사 원리를 기술하였다. 특히 원자로 내부 구조물 중 CRDM, core baffle bolt, core barrel bolt, CRGT-support pin 및 fuel alignment pin에 대한 유럽 및 독일을 중심으로 한 검사 현황 및 검사방법을 간략하게 기술하였다. 이 기술에 대한 지침안(guideline)이 독일, 프랑스, 일본을 중심으로 하여 마련되고 있다.

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Effect of Incubation Time after Cooling on the Meiotic Spindle and Chromosomes of Mouse Oocytes (냉각 후 배양시간이 생쥐 난자의 방추체와 염색체에 미치는 영향)

  • Yu I.
    • Journal of Embryo Transfer
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    • v.19 no.3
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    • pp.283-289
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    • 2004
  • This study was conducted to determine the effects of incubation time after cooling on mouse meiotic spindle and chromosome alignment and the optimal incubation time for their restoration. Oocytes at the metaphase II were obtained from superovulated mice. Control oocytes were held at 37$^{\circ}C$ during the experiment. Oocytes were rapidly cooled to $0^{\circ}C$, held for 30 minutes, warmed and incubated at 37$^{\circ}C$ for 5, 15, 30, 60 and 120 minutes, respectively. The morphological features of spindle and chromosomes in oocytes were evaluated by immunofluorescent staining. Meiotic spindle of control oocytes exhibited a normal-looking bipolar configuration(barrel-shaped) and highly fluorescent microtubles. The chromosomes were clustered in a discrete bundles at metaphase plate. Disassembly of meiotic spindle and chromosome dispersion were occurred immediately after chilling of oocyte. Fluorescence intensity index(FIS), normal chromosomes aligned and normal spindle configuration were compared according to incubation time at 37$^{\circ}C$. Restoration of a barrel-shaped spindle and normal chromosome alignment was occurring after 5 minutes incubation at 37$^{\circ}C$, improved as a incubation time increased, and decreased gradually after 120 minutes incubation(P<0.05). The optimal incubation time for restoration of meiotic spindle and chromosomes in cooled oocytes was 60 minutes.

FOCAL REDUCER FOR CQUEAN (Camera for QUasars in EArly uNiverse)

  • Lim, Juhee;Chang, Seunghyuk;Pak, Soojong;Kim, Youngju;Park, Won-Kee;Im, Myungshin
    • Journal of The Korean Astronomical Society
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    • v.46 no.4
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    • pp.161-172
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    • 2013
  • A focal reducer is developed for CQUEAN (Camera for QUasars in EArly uNiverse), which is a CCD imaging system on the 2.1 m Otto Struve telescope at the McDonald observatory. It allows CQUEAN to secure a wider field of view by reducing the effective focal length by a factor of three. The optical point spread function without seeing effects is designed to be within one pixel ($0.283^{\prime\prime}$) over the field of view of $4.82^{\prime}{\times}4.82^{\prime}$ in optimum wavelength ranges of 0.8-1.1 ${\mu}m$. In this paper, we describe and discuss the characteristics of optical design, the lens and barrel fabrications and the alignment processes. The observation results show that the image quality of the focal reducer confirms the expectations from the design.

Focal Reducer for CQUEAN

  • Lim, Ju-Hee;Chang, Seung-Hyuk;Kim, Young-Ju;Kim, Jin-Young;Park, Won-Kee;Im, Myung-Shin;Pak, Soo-Jong
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.2
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    • pp.62.2-62.2
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    • 2010
  • The CQUEAN (Camera for QUasars in EArly uNiverse) is an optical CCD camera optimized for the observation of high redshift QSOs to understand the nature of early universe. The focal reducer, which is composed of four spherical lens, is allowed to secure a wider field of view for CQUEAN, by reducing the focal length of the system by one third. We designed the lens configuration, the lens barrel, and the adapters to assemble to attach focal reducer to the CCD camera system. We performed tolerance analysis using ZEMAX. The manufacturing of the focal reducer system and its lab test of optical performance were already finished. It turned out that the performance can meet the original requirement, with the aberration and alignment error taken into account. We successfully attached the focal reducer and CQUEAN to the cassegrain focus of 2.1m telescope at McDonald Observatory, USA, and several tests of CQUEAN system were carried out. In this presentation, I will show the process of focal reducer fabrication and the result of performance test.

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The Effects of Sight Alignment Method on Pistol Shooting Records of Security Guard (경호요원 권총사격시 조준선정렬방식과 기록변화와의 관계)

  • Lee, Sang-Chul;Shin, Seung-Cheol
    • Korean Security Journal
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    • no.14
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    • pp.367-388
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    • 2007
  • Some of individuals working at various fields of security guard are given the legal right to use a gun to cope with expected risks and crimes. Not only in Korea but also throughout the world, a pistol is being used as a device for the workers of security guard such as the army, the police and intelligence agencies to keep the law and social order. A pistol, which is regarded as the last safety measure of security guard, is easy to carry but, because of its short barrel for high portability, its hit ratio and long-range shooting performance are much lower than ordinary guns. Despite such weak points, a pistol can be a great tool because of its high portability and if the users make steady efforts to improve their skills and, for this reason, it has been being used widely until now. National agency workers' pistol shooting skills depend on several important factors, but for the fast and accurate shooting, the mot important factor is how to aim at the target. Because it takes a relatively longer time to align the sight on the target, the change of the sight alignment method is expected to improve pistol shooting skills a lot. In Korea, however, most of national agency training centers teach the basic aiming method that crosses the foresight and the backsight and aligns the crossing to the target. That is, they teach the sight alignment method for fixed targets. Some agencies in Korea and foreign countries that have used guns more frequently and longer than us are teaching different sight alignment methods as well. Representative ones of them are aiming only with the foresight and pointing shooting. These aiming methods are expected to produce good results against moving targets. While the basic sight alignment method has to cross the foresight and the backsight and then to align the crossing to the target, so takes a longer time than other aiming methods, the other two aiming methods are expected to be effective in fields where both swiftness and accuracy are require. Because domestic agencies are teaching mainly the basic sight alignment method, the present study purposed to examine the effects of different aiming methods on shooting records and ultimately to contribute to national agency workers' operation. For this purpose, we selected 20 policemen from local police commandos under the National Police Agency who were accustomed to pistol shooting and measured their pistol shooting. According to the results, the pointed shooting method showed the best record in 5m short.range shooting. As to hit ratio in 15m shooting, which is relatively long.range for pistol shooting, the basic aiming method showed the best record, but the results of statistical analysis showed that pointed shooting is most superior. Of course, the results were obtained from a group who were used to and skillful in pistol shooting, but they are considered meaningful in that all the police and national agency workers should have such high shooting skills.

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