• 한국균학회소식:학술대회논문집
• /
• 2014.05a
• /
• pp.17-17
• /
• 2014

Over five thousand higher fungal specimens were collected from 32 forest areas of Chungcheong and Gyeongsang province from 2012 to 2013. We obtained 513 strains and 3,120 ITS sequences. Mushrooms were first identified with macro- and micro-scopic characters, and their identification was confirmed on the basis of ITS sequences. Voucher specimens were designated for each species found in Korea. Construction of DNA barcoding Database is currently underway with sequences of 409 species. During the development of the database, some new species were recognized, along with several Korean new records. When the system has been completed, it will provide essential molecular information for metagenomic and phylogenetic researches for higher fungi.

• Animal Systematics, Evolution and Diversity
• /
• v.36 no.1
• /
• pp.10-14
• /
• 2020

Pagurus is the most diverse hermit crab genus in Korea. In this study, the cytochrome c oxidase subunit I (COI) and 16S rRNA of 24 individuals from four Korean Pagurus species (i.e., 7 Pagurus brachiomastus, 8 P. proximus, 8 P. simulans, and 1 P. rectidactylus) were sequenced and analyzed. No genetic difference was found between the COI and 16S rRNA sequences of P. brachiomastus and P. simulans, and the COI sequences of P. rectidactylus and P. quinquelineatus (comparative species from NCBI). Considering the morphological and ecological characteristics together, we assume that P. simulans and P. rectidactylus are subspecies of P. brachiomastus and P. quinquelineatus, respectively. This study should facilitate further research on the taxonomic status of these species.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.31 no.2
• /
• pp.40-47
• /
• 2015

The genus Acteniceromorphus is taxonomically reviewed for the first time in Korea. From the previously recorded three species, two species, A. selectus (Candèze, 1894) and A. fulvipennis (Lewis, 1894) are confirmed as misidientification of species which are endemic to Japan. Another species, A. chlamydatus (Lewis, 1894) is unavailable any Korean specimens. Additionally, we found A. ferrugineipennis (Candèze, 1879) as new to Korea. We also provide a comparison of DNA barcoding for two species previously misidentified and the newly recorded species, except A. chlamydatus. DNA barcoding result shows that each species is clearly delimited at species-level from each other by large interspecific genetic distance over 7.2%.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.5
• /
• pp.794-799
• /
• 2020

In April 2019, a single Peristediidae juvenile (14.1 mm SL) was collected from the waters off of Jeju-do Island, Korea. DNA barcoding identified the juvenile as Peristedion liorhynchus, an unrecorded species in Korea. P. liorhynchus has eight dorsal fin spines, 22 dorsal and 19 anal fin rays, and a long third pectoral fin ray that passes through the middle of the tail at the juvenile stage. Juvenile also have large heads, extensive head spination, and serrated edges on the ocular and parietal spines. This is the first record of P. liorhynchus in Korea; therefore, we propose the new Korean name, "Nam-bang-hwang-seong-dae".

• Animal Systematics, Evolution and Diversity
• /
• v.37 no.4
• /
• pp.354-357
• /
• 2021

In Korean waters, the cirolanid isopod, Eurydice longiantennata Nunomura and Ikehara, 1985 has been reported only from the subtidal zone of Jeju island. We obtained the mitochondrial cytochrome c oxidase subunit I (COI) sequences of this species and determined the DNA barcoding data of E. longiantennata based on a genetic comparison of E. longiantennata and its congeners. The intra-specific genetic distance between the three COI sequences of E. longiantennata ranged from 0 to 0.6%. The inter-specific distances between E. longiantennata and other cirolanid isopods ranged from 24 to 33.2%. In this study, we provided the DNA information of E. longiantennata with a morphological diagnosis and images of the species.

• Animal Systematics, Evolution and Diversity
• /
• v.35 no.1
• /
• pp.30-32
• /
• 2019

The brachyuran crab Bythograeidae Williams, 1980 is common in hydrothermal vent fields worldwide and has recorded to sixteen species of six genera. In this study, we firstly determined the cytochrome c oxidase subunit 1 (CO1) DNA barcodes for the fifth species of Austinograea, A. hourdezi, from hydrothermal vent regions of the North Fiji Basin in southwestern Pacific Ocean. All CO1 DNA barcodes of A. hourdezi were identical. The interspecies variations of three bythograeid genera were 10.9-13.3% for Austinograea, 6.6-15.7% for Bythograea, and 9.7% for Gandalfus. These results would be helpful to understand taxonomy of brachyuran crabs living in hydrothermal vent fields using CO1 DNA barcodes.

• Animal Systematics, Evolution and Diversity
• /
• v.35 no.3
• /
• pp.136-139
• /
• 2019

The pulmonate gastropod Ellobium chinense (Pfeiffer, 1864) is an endangered marine species along the South Korean coasts due to habitat destruction and population declines. We sequenced the cytochrome c oxidase subunit 1 (COI) of 25 E. chinense specimens collected from five coastal sites in South Korea, and identified 16 unique haplotypes. The maximum intraspecific variation among individuals was 1.6%, while interspecific differences from another ellobiid species, Auriculastra duplicata (Pfeiffer, 1854), ranged from 21.9 to 23.0%. Our barcoding data will be useful to elucidate the phylogenetic relationships among pulmonate gastropods and infer the population genetic structure of E. chinense.

• Animal Systematics, Evolution and Diversity
• /
• v.35 no.3
• /
• pp.156-159
• /
• 2019

The phyllodicd polychaete species, Nereiphylla hera Kato and Mawatari, 1999 is reported from the intertidal habitats of the eastern coast of South Korea. We determined the DNA barcoding region of the mitochondrial cytochrome c oxidase subunit I (COI) of N. hera and compared nucleotide variation with its congeners. The intra-specific genetic distance between the three COI sequences of N. hera was ranged from 0 to 0.4%. The inter-specific distances between N. hera and other Nereiphylla species ranged from 18.8 to 22.3%. In this study, we reported the first COI barcodes of N. hera with the morphologcial diagnosis and the photographs. These results would be helpful to understand taxonomy of Nereiphylla.

• Animal Systematics, Evolution and Diversity
• /
• v.37 no.1
• /
• pp.26-30
• /
• 2021

Botrylloides violaceus is native to the Northwest Pacific, including Korea. This species has many color variations in alive condition and a variety of zooid compound forms, and therefore difficult to identification in the field survey. This is the first report of COI DNA barcodes of B. violaceus from Korea. The intra-specific pairwise distance between Korean and UK populations had ranged from 1.4% to 2.6%. The inter-specific variations between B. violaceus and other Botrylloides species were 21.0-36.8%. The new DNA barcodes for Korean B. violaceus may be helpful in the identification of colonial ascidians, which is a difficult task when based on morphological identification.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.54 no.3
• /
• pp.340-349
• /
• 2021

Three specimens (33.6-44.8 mm in standard length) of the family Bregmacerotidae, collected from the waters off south-western Jeju-do Island in October 2016, were identified as Bregmaceros nectabanus on the basis of morphological and molecular methods (DNA barcoding). This species is characterized by having 50-52 2nd dorsal fin rays, 52-53 anal fin rays, 13 principal caudal fin rays, an unpigmented abdomen, irregularly distributed dorsal chromatophores, and the origin of a dorsal fin slightly behind the anal fin. This is the first reliable record of B. nectabanus in Korea. According to Yoo et al. (1992), we propose a Korean name "Tae-Pyeong-Yang-Nal-Gae-Myeol" for this species. Also, we suggest key to species of these eight bregmacerotids from the northwestern Pacific.