• 한국버섯학회지
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• 제7권2호
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• pp.45-48
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• 2009

느타리버섯 재배에 있어서 부레옥잠과 바나나 잎, 줄기는 단독으로 배지재료로 쓰기는 어려운 것으로 나타났다. 면실피를 주재료로 하고 부레옥잠과 바나나 잎, 줄기를 첨가재료로 사용할 경우 부레옥잠을 20% 혼합하였을 때 115mm로 대조구와 같은 균사생장속도를 나타냈고 50%를 혼합하였을 때 103mm로 균사생장속도는 약간 느리지만 균사밀도가 높아 50%까지 대체 사용할 수 있음을 확인하였다. 바나나는 20%를 혼합하였을 때 106mm의 생장속도를 보였고 그 이상의 혼합비에서는 균사생장속도가 늦어지고 균사밀도가 약해졌다. 이는 부레옥잠에서는 주재료의 대체 비율이 20%이상 50%미만 이었고, 바나나 잎, 줄기에서는 20%미만 이었다. 부레옥잠과 바나나 잎, 줄기는 대체배지로 그 생산량이 많고 저가로 구입할 수 있기 때문에 앞으로 더 많은 연구와 실증재배를 해 볼 필요가 있다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.245-247
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• 2005

A cellulolytic fungus isolated from Agave plantation in northeastern Thailand was identified as Acrophialophora nainiana. The fungus was capable of growing at pH between 3 - 7 and 25 - 45 $^{\circ}C$, with the optimum conditions at pH 5.0 and 40 $^{\circ}C$. The wild isolate produced cellulases, comprising of exoglucanase (0.019 U/mg protein), endoglucanase (0.366 U/mg protein), and ${\beta}$-glucosidase (0.001 U/mg protein). Mutations with UV and NTG produced the UV 10-2 mutant with cellulases activities including exoglucanase (0.093 U/mg protein), endoglucanase (0.585 U/mg protein), and ${\beta}$-glucosidase (0.013 U/mg protein). Purification of the enzymes with ultrafiltration, ammonium sulfate precipitation, and ion-exchange chromatography yielded the maximal cellulase specific activities of 2.736 U/mg protein (exoglucanase), 0.235 U/mg protein (endoglucanase), and 0.008 U/mg protein (${\beta}$-glucosidase). The mutant's cellulases were the most active at pH 5.0 and 60 $^{\circ}C$. Ion-exchange chromatography revealed that A. nainiana UV 10-2 cellulases were comprised of two peaks with one peak showing the single endoglucanase activity while the other peak showed a mixture of the three enzyme activities. Production of A. nainiana UV 10-2 cellulases using banana leaf stalk as the sole carbon source gave comparable yields to that of the pure ${\alpha}$-cellulose. The enzymes were used in the simultaneous saccharification and fermentation (SSF) of plant residue (Coix aquatica) along with Kluveromyces marxianus to produce ethanol. Moreover, when the enzymes were used in the bioscouring process of fabric, the desiravle traits of textile processing including immediate water absorbency, increased in whiteness and reduction of yellowness of the treated fabric were observed.