• Title/Summary/Keyword: Bacteriological study

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Occurrence Of Fecal Pollution Bacteria In The Water Of Lake Eui-Am (의암땜 하류수역의 분변성 오염세균류의 분포)

  • Choe, Sang;Kim, Geon Chee
    • 한국해양학회지
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    • v.5 no.2
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    • pp.59-64
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    • 1970
  • The purpose of the study was to gather basic bacteriological data regarding the quality of Korean surface waters. Total coliforms, fecal coliforms and fecal Streptococci survey in the water of Lake Eui-Am were undertaken by the membrance filter technique. A total of 37 samples were collected in August 14, 1970. Total coliforms were detected in all samples, fecal coliforms and fecal Streptococci were detected in 68% and 82% of samples, respectively. Bacterial densities of the lake water were varied by station and depth. The numbers of bacteria per 100ml of the lake water were; 8-225(65.3 average) for the total coliforms, 0-112(26.2 average) for the fecal coliforms, 0-77(25.8 average) for the fecal Streptococci, and 8-302 (91.1 average) for the total number of total coliforms and fecal Streptococci. These results suggest that the Eui-Am lake water is only lightly polluted and indicate that the lake water, properly maintained, is a source of raw water of good bacteriological quality. Three forms of fecal pollution bacteria tend to increase with depth. This is believed that the suspended matter with conglomerated bacteria plays an important role in regulating of bacterial densities in summer season.

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Assessment of the Bacteriological Safety of Seawater and Oysters Crassostrea gigas in Yongnam-Gwangdo Area, Korea 2014-2016 (2014-2016년 경남 용남·광도해역 해수 및 굴(Crassostrea gigas)의 세균학적 위생평가)

  • Yoon, Hyun Jin;Kwon, Ji Young;Lee, Ka Jeong;Kwon, Soon Jae;Mok, Jong Soo;Kim, Poong Ho;Jung, Yeoun Joong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.5
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    • pp.535-541
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    • 2018
  • This study evaluated the sanitary state of seawater and shellfish in Yongnam-Gwangdo area from January 2014 to December 2016. The sampling stations for sanitary survey in Yongnam-Gwangdo area were composed of 41 seawater stations and 5 oyster Crassostrea gigas stations. The samples were collected monthly at each station. Shellfish-borne disease is associated with bacteria and viruses in the presence of fecal coliforms. Bacteriological pollution levels of shellfish increase with seawater quality. Therefore, fecal coliforms are very important criteria for evaluating the safety of fisheries in coastal areas. The geometric mean and the estimated 90th percentile ranges of total and fecal coliforms for seawater were 1.8-11.7, 2.8-233.6, <1.8-6.7 and 1.8-100.2 MPN/100 mL, respectively. The range of E. coli levels for oysters was <20-1,300 MPN/100 g. Based on various standards, the sanitary condition in Yongnam-Gwangdo area was evaluated as follows: clean area (Korean criteria), conditionally approved area (US criteria) and class B (EU criteria).

A Bacteriological Study on the Sea Waters and Oyster in Puk Ma , Korea (북만의 해수 및 굴의 세균학적 특성)

  • Park, Jong-Duck;Jeong, Woo-Geon
    • The Korean Journal of Malacology
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    • v.14 no.1
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    • pp.19-26
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    • 1998
  • 북만 해수의 물리 화학적 및 미생물학적 특성과 북만에서 양식되고 있는 굴에 대한 세균학적 수질을 조사하여 수출용 패류생산지정 해역수질에 합당한가를 파악함과 동시에 지표세균의 조성, 병원성 세균 등을 조사한 결과를 요약하면 다음과 같다.사 기간중 불반 해수의 수온은 6.8 -23.$0^{\circ}C$, 투명도는 2.7-5.9m, COD 1.55-2.12 mg/1, DO 6.3-10.1 mg/1, 용존질소 1.63-20.87 $\mu\textrm{g}$-at/1, 인산염 0.16-1.66 $\mu\textrm{g}$-at/1, Chlorophyll. a는 1.04-6.78 mg/ ㎥범위였으며 염분농도는 31.57-34.47$\textperthousand$였다. 북만 해수의 세균학적 수질은 통영항, 인평포, 법송포 및 북신만 안쪽의 일부 해역을 제외하고는 수출용 패류의 생산해역의 수질기준에 합당하였다. 대장균군의 최확수는 양식을 하지 않는 조사지점 1과 2를 제외하고 해수 100ml 당 < 3.0-4,600의 범위였으며 230을 초과하는 시료의 비율은 10.7%였고, 분변계 대장균의 최확수는 <3.0-1,100의 범위였으며 43을 초과하는 시료의 비율은 9.5%로 한계치 10%를 약간 상회하였다. 대장균군의 분류겨로가 Escherichia coli가 약 52%나 되어 오염원의 주류가 분변오염임을 알 수 있었다. 살모넬라, 시겔라, 콜레라균 등 수인성 병원세균은 검출되지 않았다. 병원성 비브리오균은 여름철인 6-8월 사이에는 시료의 9-22%에서 양성으로 나타났다. 굴 내에 생균수는 2.0 x $10^{2}$-1.6 x $10^{3}$범위였고 대장균군의 최확수는 <18 - 16,000, 중앙치는 176, 분변계 대장균은 <18-2,400, 중앙치는 었다.

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Clinical Bacteriological and Observations on Infectious Bovine Keratoconjunctivitis (IBK) in an Integrated Dairy Farm (대단위(大單位) 낙농목장(酪農牧場)에서의 소전염성결막각막염(傳染性結膜角膜炎)(IBK)의 임상적(臨床的) 및 세균학적(細菌學的) 관찰(觀察))

  • Seok, Ho-bong
    • Korean Journal of Veterinary Research
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    • v.25 no.1
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    • pp.53-59
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    • 1985
  • A group of 80 Holstein calves, many with clinical signs of infectious bovine keratoconjunctivitis in summer seasons, was assembled for bacteriologic and ophthalmologic studies at an integrated dairy farm. Observations were started from 1980 to 1984 but bacteriological study was only carried out from thor spring in 1981 and continued during the Autumn in 1981. Corneal lesions and drugs sensitivity were also observed and the results obtained were as follows: 1. Occurrences of IBK-affected cattle during the 5 years were predominent to calves in summer. 2. Among 142 eyes from 80 cattle, 53 isolates of Moraxella bovis(37.3%) were isolated from 37 cattle(46.3%). The prevalence of the infection for clinical eyes were higher than nonclinical eyes calves. 3. Miscellaneous organisms were found from age groups of 5 months to 9 months old. It were 8 hemolytic Neisseria spp., 3 Fungi, Mycoplasma sp. and 1 Pseudomonas sp., but 9 samples were unknown. 4. Prevalence of infection with M. bovis in bilateral clinical eyes were higher than unilateral eyes. 5. Incidence of corneal lesion was predominent in early stage as a watery tears(21.0%) and late stage as a leukomas of corneal opacity(22.8%). 6. The number of isolations of M. bovis and incidence of IBK varied from year to year; Higher incidence of IBK clinical signs were showed in calves from 5 months to 10 months old and the number of isolations of M. bovis was declined in order 8 months(100.0%), 4 months(75.0%), 5 months(66.6%) and 8 months(66.6%) old calves. 7. Chloramphenicol, Nitrofurantoin and Cephalothin were highly sensitive against M. bovis isolates and then Tetracycline, Neomycin, Erythromycin and Kanamycin were intermediate but low sensitive to Streptomycin, Colistin and Penicillin.

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Pathological, immunohistochemical, and bacteriological findings in dogs infected with Brucella canis

  • Jung, Ji-Youl;Yoon, Soon-Seek;Lee, Seunghee;Park, Jung-Won;Lee, JinJu;Her, Moon;So, ByungJae;Kim, Jae-Hoon
    • Korean Journal of Veterinary Research
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    • v.60 no.1
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    • pp.9-14
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    • 2020
  • This study describes pathological, immunohistochemical, and bacteriological findings in adult dogs and fetuses naturally infected with Brucella (B.) canis. A total of 42 dogs including 40 dogs and 2 aborted fetuses were examined. The most common gross lesions in infected dogs were swelling of lymph nodes and spleen. The testes showed marked swelling with multifocal to diffuse reddish discoloration. The most significant histopathological lesions were observed in the placenta. Placental trophoblasts were markedly hypertrophied due to the accumulation of intra-cellular gram-negative bacteria. Lymphocytic inflammation of varying severity was observed in the reproductive organs such as male testis, epididymis, and prostate gland and female uterus. Strong immunolabelling was observed in the cytoplasm of most trophoblasts in the placental tissues using immunohistochemistry. However, immunohistochemical staining did not demonstrate any organisms in other organs of dogs and fetuses. B. canis isolates were most frequently obtained from the whole blood (82.5%) and superficial inguinal lymph node (77.5%) in both sexes. In addition, the isolation rate was higher in male genital organs than in those of females. Hence, management of male dogs is most important because infected dogs can play a role as carriers.

Clinical study of empyema thoracis (농흉의 임상적 연구)

  • 남구현
    • Journal of Chest Surgery
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    • v.16 no.1
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    • pp.171-175
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    • 1983
  • The authors made a clinical study of 107 cases of empyema who were treated at Department of Thoracic Surgery of Chungnam National University Hospital during the period of Jan. 1976 through Aug. 1982 and compared the empyema of infant and children with that of adults. Following was the remits: 1. Male was predominant to female with the ratio of 2.5 to 1 and adult to infant was 2.6 to 1. 2. The cardinal symptoms were dyspnea, coughing, chest pain and fever. 3. Positive remit of bacteriological culture study was reported in 57% and the most common causative organism was staphylococcus in infant and childhood, E. coil in adult. 4. Most frequent predisposing factors of thoracic empyema in infant and childhood was pneumonia [53%], and in adult was pulmonary tuberculosis [40%]. 5. Treatment were combined with antibiotics therapy and several surgical procedures for empyema. 24 cases [22.4%] required open thoracotomy. 6. The mortality rate was 7.4% [8/107]. 86 cases [80.4%] were discharged with recovery and improvement.

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Clinical Study of Empyema Thoracis (I) (농흉의 임상적 고찰 (제 1보))

  • 유회성
    • Journal of Chest Surgery
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    • v.4 no.2
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    • pp.95-100
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    • 1971
  • The incidence of empyema has been drastically reduced with the advent of antimicrobial drugs,however,there is still complicated and difficult problems of management of empyema remaining. During the period of December, 1958 to December, 1962, 90 patients nf empyema thoracis were managed in the Department of Thoracic Surgery of the National Medical Center, and this series deals with the incidence,etiologic consideration, bacteriology and management of empyema with its result. 1] Male predominates with the ratio of 3.1: 1, and peak age incidence lies in 3rd decade. 2] Most common etiologic factor is bronchorespiratory infection among which tuberculosis remains highest incidence. 3] 56.7% of patients shows positive result of bacteriologic study and about half of positive culture series shows mixed infection or changing pattern of bacteriological strains during serial examinations. 4] Complete cure is obtained in 84.4% of patients with 5 deaths.

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Study on the Identification of Enteropathogenic Bacteria from Diarrheal Patients (설사 환자에서의 원인균 분리동정에 관한 연구)

  • Jun, Sung-Sook;Seo, Su-Yung;Kim, Yung-Bu;Oh, Yang-Hyo;Yang, Hak-Do
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.4
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    • pp.417-422
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    • 1986
  • The specimens were collected from 89 diarrheal patients who had visited Pusan National University Hospital from June to September 1985. They were cultured and tested for the bacteriological identification of causative agents. In this study we identified 5 strains of Salmonella species, 5 strains of Shigella species, 2 strains of Y. enterocolitica, and 17 strains of enteric pathogenic E. coli. Enteric pathogenic E. coli were classified into enterotoxigenic E. coli, enteropathogenic E. coli, and enteroinvasive E. coli by serological type. We tried to isolate V. cholerae and V. parahaemolyticus too but we cannot find them out.

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Hygienic Status of Kitchen Utensils used at College Student's Home in Seoul City (서울시내(市內) 대학생가정(大學生家庭)에서 사용(使用)하는 식품(食器)의 위생학적(衛生學的) 조사(調査))

  • Chung, Kyu-Sun
    • Journal of Nutrition and Health
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    • v.4 no.3
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    • pp.13-25
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    • 1971
  • During a period from October 1st to November 15th in 1971, We made a survey on hygienic status of kitchen utensils used at 270 college student's home in 9 districts of Seoul city. The results obtained could be summarized as follows. In bacteriological study on the kitchen utensils 20.7% of them revealed contaminations with general bacterial counts of over 100/m1 and 20.0% of them with coliform bacteria. It was probable that degrees of bacterial contamination of the kitchen utensils were chiefly attributable to levels of education of housewives and living standard of individual household. Factors that were presumed to be directly responsible for the contamination were sources and storage of water and methods of washing the utensils. One of the major sources of contamination was derived from the use of a piece of cloth (Hangjoo) to wipe up the utensils after soashing. Hygienic treatment of Hangjoo in urgently needed. Otherwise, it is desirable not to used the Hangjoo and leave the utensils dry after washing them with boiling water.

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A Study on the Effects of Livestock Wastewater on Stream Pollution(with Bacteriological Examination) (축산폐수가 하천오염에 미치는 영향에 관한 연구 (하) -세균학적 조사를 중심으로-)

  • Ryu J. K.;Lee H. S.;Lee W. C.;Kim T. J.;Lee T. J.
    • Journal of the korean veterinary medical association
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    • v.22 no.4
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    • pp.238-245
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    • 1986
  • To evaluate effects of livestock wastewater on stream pollution, the survey on the environmental sanitation, hydrology, water quality, Pollutant loads and bacterial indicators in a stream receiving livestock wastewater discharged from Chun Sung livestock

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