• Title/Summary/Keyword: Bacterial size

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Isolation of a Bacterium That Inhibits the Growth of Anabaena cylindrica

  • Kim, Chul-Ho;Leem, Mi-Hyea;Choi, Yong-Keel
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.284-289
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    • 1997
  • A Gram (-), rod-shaped bacterium $2.3{\sim}2.8{\times}0.45{\mu}m$ in size which exhibited growth-inhibiting effects against a cyanobacterium (Anabaena cylindrica) was isolated from Daechung Dam Reservoir. This isolate was identified as Moraxella sp. and designated Moracella sp. CK-1. Hollow zones formed around bacterial colonies on the cyanobacterial lawn. In a mixed-culture of A. cylindrica and the isolate, each microorganism grew inverse-proportionally, and the cyanobacterial vegetative cells completely disappeared within 24 hours. On treatment with Moraxella sp. CK-1, cell walls of A. cylindrica disappeared, but sheathes remained in a more electron dense form. The unit membrane such as thylakoidal membrane was stable to bacterial lysing activity. This bacterium showed a broad action spectrum against cyanobacteria. The growth-inhibiting activity of Moracella sp. CK-1 against A. cylindrica is believed to be performed through the excretion of active substances.

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Antimicrobial Action of Dental Cements (치과용 시멘트의 항미생물 작용)

  • Kim, Cheol-We;Seonoo, Young-Gook;Paik, Dae-Il;Kim, Jong-Bai;Choe, Son-Jin
    • The Journal of the Korean dental association
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    • v.21 no.7 s.170
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    • pp.573-577
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    • 1983
  • The antimicrobial action of various dental cements evaluated against common micro-organisms most frequenty found within the components of the normal bacterial flora of oral cavity. They include Streptococcus mutans (2 strains), Lactobacillus acidophilus, Actinomyces viscosus, and Streptococcus sanguis. The test was done by the use of brain heart infusion (BHI) agar plates. A standard mix of each cement was made and placed on the plates which were seeded with a standard culture of microorganisms. After incubation, the halo of bacterial growth inhibition around the cement was identified and its size was measured. Some of the cements tested had obvious antibacterial effect. The cements listed in decreasing order of effectiveness are 1) zinc phosphate and oxyphosphate, 2) silicate, 3) zinc oxide-eugenol, 4) calcium hydroxide, and 5) carboxylate.

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Distribution and Activity of Hheterotrophic Bacteria in the Mudflat of Nakdong River Estuary (난동감 하구 간석지에 존재하는 세균의 분포 및 생리적 활성도)

  • Kim, Sang-Jong;Hong, Soon-Woo;Rhie, Youn;Choi, Sung-Chan
    • Korean Journal of Microbiology
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    • v.23 no.3
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    • pp.215-222
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    • 1985
  • Distribution pattern and activity of heterotrophec bacteria were measured in the mudflat of Nakdong river estuary. In March and June, 1985, community sizes of amylolytic, lipolytic and proteolytic bacteria as well as total viable counts were measured. Vertical distribution of bacterial community size increased a few orders of magnitude from Narch to June. Heterotrophic activity was estimated in turnover time with $U-[^{14}C]-glucose$. Turnover time reduced considerably in June compared to that of March. To sxamine correlations for measured bacterial groups, turnover time and environmental factors, correlation coefficient matrix was obtained. These measured characteristics did not consistently correlate well with one another.

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Disinfection Efficacy of an Ultraviolet Light on Film Cassettes for Preventive of the Nosocomial Infection (병원감염 예방을 위한 Film Cassette의 자외선 소독 효과)

  • Kweon, Dae-Cheol;Jeon, Yong-Woong;Cho, Am
    • Journal of radiological science and technology
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    • v.24 no.1
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    • pp.27-32
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    • 2001
  • The bacteria infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient prevention from nosocomial infection and for improvement of the hospital environment. The laboratory result was identified non-pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection is proven suitable for bacteria. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In addition education of nosocomial infection for radiographers will be required. In conclusion, ultraviolet is considered effective to irradate bacteria. Additionally, two minutes are required to sterilize film cassettes.

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Property Evaluation of Breathable Blend Fabric of MPCE Copolymer and Wet Coagulated Polyurethane (MPCE copolymer와 습식 폴리우레탄의 블렌드에 의한 투습방수 나일론 직물의 제반 물성변화에 관한 연구)

  • Yi, Jong-Woo;Chae, Ei-Jung;Bae, Jin-Seok
    • Textile Coloration and Finishing
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    • v.26 no.4
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    • pp.322-330
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    • 2014
  • The phospholipid-based MPCE(2-Methacryloxyethyl Phosphoryl Chlorine) copolymer was mixed with wet polyurethane for coating of nylon fabrics. The substitution rate of water in coagulation bath with DMF was changed under control of the size of formed hydrophilic microporous cell enable to manufacture excellent breathable, anti-bacterial and moisture control fabrics. Biocompatible property, vapor permeability, hydrostatic pressure, moisture management and anti-bacterial property were investigated for treated nylon fabrics. In result, increased moisture transmission rates, decreased water resistance and outstanding moisture control property could be confirmed by enhanced hydrophilicity of wet-coated nylon fabric with MPCE copolymer.

A Study on Contamination and Disinfection of Film Cassette (Film Cassette의 세균 오염도와 소독에 관한 연구)

  • Kweon, Dae-Cheol;Chung, Kyung-Mo;Choi, Ji-Won
    • Journal of radiological science and technology
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    • v.23 no.2
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    • pp.55-61
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    • 2000
  • In July 2000, a bacteria infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient to prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic bacterial in the four different cassette size of the contact surface. The study concludes that presence of a bacterial infection wilt prevent a using antiseptic technique on film cassette contact surface. Also the education of nosocomial infection for radiographer will be required.

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The Experimental Study on Anti-bacterial Potency of Jinpi-san on Staphylococcus aureus & Staphylococcus epidermidis. (진피산(秦皮散)이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.1 s.32
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    • pp.195-200
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Jinpi-san on Staphylococcus aureus(S. aureus) and Staphylococcus epidermidis(S. epidermidis) that induce keratitis. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Jinpi-san(100%, 10%, 1%, 0.1 %). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on S. aureus in Jinpi-san was $40{\mu}l$ undiluted solution. 2. MIC on S. epidermidis in Jinpi-san was $20{\mu}l$ undiluted solution. Conclusions : These results indicate that Jinpi-san can be used to cure S. aureus, S. epidermidis that induce eye disease(keratitis). If further study is performed, the use of eye drops will be valuable and beneficial in the clinical medicines.

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The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture on Staphylococcus aureus & Staphylococcus epidermidis (소염 약침액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.73-77
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture(100%, 10%, 1%, 0.1%). Antibacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture disappear all sample. Conclusions : The present author think that Soyum Herbal-acupuncture must be extract a new method for anti-bacterial potency.

Change in Cell Size and Buoyant Density of Pseudomonas diminuta in Response to Osmotic Shocks

  • Lee, So-Hee;Cho, Yu-Ree;Choi, Yong-Jin;Kim, Chan-Wha
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.326-328
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    • 2001
  • Pseudomonas diminuta (ATCC 19146) has been typically used in the bacterial challenge test for validation of the sterilizing filtration process. Cell size is critical for determining the retention characteristics of membrane filters with pore-size of $0.2{\mu}m$. The changes of cell sizes after osmotic shocks at 150, 260, 500, and 700 mosM were measured by a particle size analyzer and the changes of their buoyant densities were analyzed with a Percoll gradient. The results indicated that there were no significant differences when cells were cultured in 260 mosM medium and osmotically shocked at 500 and 700 mosM. However, the osmotically shocked cells at 150 mosM showed a 38% increase of the cell size compared to the cells at 260 mosM. From these study, we concluded that the worst case condition for validation of a sterilizing filter would be 500 mosM, not because of changes in the cell size, but due to decrease in cell viability under those conditions.

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Distribution and Properties of Microorganisms in Soil of Representative Vegetation of Mt. Nam (남산 주요 식생의 토양 미생물의 분포 및 생리적 특성)

  • 성치남;백근식;김종홍;전영문;김정근
    • The Korean Journal of Ecology
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    • v.21 no.5_3
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    • pp.703-712
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    • 1998
  • Physicochemical factors, microbial population size and the properties of the bacterial isolates were estimated to find out the nature of soil ecosystem of Mt. Nam. Samples were obtained from the surface layer of soils on which specific plant community is developed. Average content of moisture and organic matter of the soils were 21.6% and 17.3%, respectively. These values were similar to those of developing forest soils, but were slightly lower than those of climax ecosystem such as Piagol in Mt. Chiri. Chiri. Content of phosphate was higher than those of other forest soils. The population size of soil bacteria ranged from 27.4 to 195.8 ${\times}\;10^5$ CFU/g. duy soil, and the size is somewhat dependent on the moisture and oranic matter content of soils. A large number of bacteria were able to decompose macromolecules such as starch, elastin and gelatin. Bacterial species composition of each soil was comparatively simple. Pseudomonas, Agrobacterium, Flavobacterium and Xanthomonas which are Gram-negative short rods were widely distributed in the forest soils. The endospore forming Bacillus species were also the main constituents of the soil microflroa. Actinomycetes were widely distributed in the forest soils, but the distribution pattern varied in each site. Most of the actinomycetes were also able to decompose organic macromolecules. The rate of resistant actinomycete strains to antibiotics and heavy metals were lower than those from cultivated soils, but higher than those from well-preserved forest soils. Antibiosis pattern of the actinomycete isolates was similiar to the resistance pattern. This means the forest soils of Mt. nam was somewhat interferred by artificial behabiour.

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