• Title/Summary/Keyword: Bacterial Se

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Pleuritis and pericarditis associated with Klebsiella pneumoniae in a Eurasian beaver (Castor fiber)

  • You, Mi Hyeon;Kim, Ji Hyung;Kim, Dae Yong;Gomez, Dennis Kaw;Jung, Tae Sung;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.48 no.4
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    • pp.501-503
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    • 2008
  • An adult one-year-old male Eurasian beaver (Castor fiber) died of a traumatic injury to its right leg from a fall. At necropsy, fibrinopurulent exudates were observed in the thoracic cavity. Histopathologic examination showed that the pericardium, thoracic wall, pulmonary pleura and the lungs were markedly thickened due to mixtures of necrotic cellular debris, neutrophils, fibrin, red blood cells, and bacterial aggregates. Pure culture of Klebsiella (K.) pneumoniae was isolated from the thoracic exudates, pleura and heart tissues. Based on these findings, this is the first report describing pleuritis and pericarditis associated to K. pneumoniae in a beaver.

Comparison of two diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR to discriminate between Salmonella enterica serovars Gallinarum and S Pullorum (Salmonella enterica serovars Gallinarum과 S Pullorum의 감별을 위한 2가지 진단법: allele-specific real-time PCR과 3'-tailed PCR의 비교)

  • Lee, Se-Mi;Seo, Ja-Young;Lee, Jae-Il;Kim, Tae-Jung
    • Korean Journal of Veterinary Service
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    • v.31 no.4
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    • pp.485-492
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    • 2008
  • Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms ($237^{th}\;and\;598^{th}$). In both methods, $237^{th}$ allele was found to be a good target for differential diagnosis, while $598^{th}$ allele produced some non-specific reactions.

Functional Implications of the Conserved Action of Regulators of Ribonuclease Activity

  • Yeom, Ji-Hyun;Shin, Eun-Kyoung;Go, Ha-Young;Sim, Se-Hoon;Seong, Maeng-Je;Lee, Kang-Seok
    • Journal of Microbiology and Biotechnology
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    • v.18 no.8
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    • pp.1353-1356
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    • 2008
  • RNase E (Rne) plays a major role in the decay and processing of numerous RNAs in E. coli, and protein inhibitors of RNase E, RraA and RraB, have recently been discovered. Here, we report that coexpression of RraA or RraB reduces the ribonucleolytic activity in rne-deleted E. coli cells overproducing RNase ES, a Streptomyces coelicolor functional ortholog of RNase E, and consequently rescues these cells from growth arrest. These findings suggest that the regulators of ribonuclease activity have a conserved intrinsic property that effectively acts on an RNase E-like enzyme found in a distantly related bacterial species.

Nonsurgical interventions for treating peri-implantitis and prognosis (임상가를 위한 특집 1 - 임플란트 주위염의 비외과적 치료 방법과 예후)

  • Park, Se-Hwan;Lee, Jae-Kwan
    • The Journal of the Korean dental association
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    • v.52 no.7
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    • pp.396-401
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    • 2014
  • Peri-implantitis is an inflammatory disease of the peri-implant tissue by bacterial infection or other factors, which results in peri-implant bone loss. Many nonsurgical treatments were tried on initial to moderate peri-implantitis lesion to reduce the inflammation. Some of these treatments made effective results, however, they were not definitively predictable. To prevent peri-implantitis and further peri-implant bone loss, early intervention is the most important. Early detection of peri-implant infection through the regular maintenance care can make it possible to do early nonsurgical intervention. Nonsurgical intervention is effective on peri-implant mucositis and can also be effective on initial peri-implantitis lesion. If the peri-implantitis is not resolves by nonsurgical treatment, surgical approach should be considered.

Quinolone Alkaloids from the Arctic Bacterium, Pseudomonas aeruginosa (북극해 박테리아, Pseudomonas aeruginosa에서 분리된 퀴놀론 알칼로이드)

  • Youn, Ui Joung;Han, Se Jong;Kim, Il Chan;Yim, Jung Han
    • Korean Journal of Pharmacognosy
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    • v.49 no.2
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    • pp.108-112
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    • 2018
  • Four quinolone alkaloids, 2-heptyl-4-quinolone (1), 2-nonyl-4-quinolone (2), 2-undecyl-4-quinolone (3), and 2-undecen-1'-yl-4-quinolone (4), together with two nitrogen derived benzoic acid derivatives, N-acetylanthranilic acid (5) and o-acetamidobenzamide (6) have been isolated from the Arctic bacterial strain, Pseudomonas aeruginosa. The structures of the compounds were determined by 1D and 2D NMR, and MS experiments, as well as by comparison of their data with published values. To the best of our knowledge, compounds 3-6 were isolated for the first time from P. aeruginosa.

Biological control of Flavobacterium psychrophilum infection in ayu (Plecoglossus altivelis altivelis) using a bacteriophage PFpW-3

  • Kim, Ji Hyung;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.58 no.1
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    • pp.39-43
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    • 2018
  • The efficacy of using a bacteriophage (phage) to control Flavobacterium psychrophilum (F. psychrophilum) infection of ayu (Plecoglossus altivelis altivelis) was evaluated in this study. Intramuscular challenge failed to induce sufficient infection levels; therefore, a newly designed net-scratch challenge method was also used to induce bacterial infection. Administration of phage PFpW-3 in F. psychrophilum-infected ayu showed notable protective effects, increased survival rates and mean times to death. Additionally, the fate of inoculated bacteria and phage in ayu were investigated. Our results suggest that the phage PFpW-3 could be considered an alternative biocontrol agent against F. psychrophilum infections in ayu culture.

Coating using chitosan as a means to prevent dispersion of moist pellet feed in water of aquacultural farm, and bacterial pollution and its lipid oxidation during storage

  • Lee, Ki-Wan;Jeon, You-Jin;Heo, Moon-Soo;Song, Choon-Bok;Ha, Jin-Hwan;Kim, Se-Kwon
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.149-150
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    • 2001
  • A fish feed being used most widely in aquacultural farm is a moist pellet (MP) feed which has been being prepared by mixing of commercial dry feed and nutritional oil to raw fish ky. The two major problems in the use of MP feed is to be readily dispersed in water of an aquacultural farm, and to cause lipid oxidation and bacteria infection during storage. The former induce an environmental pollution of the farm and the latter lower nutritional values and develop fish diseasees. (omitted)

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Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum

  • Han, Jee Eun;Gomez, Dennis K.;Kim, Ji Hyung;Choresca, Casiano H. Jr.;Shin, Sang Phil;Baeck, Gun Wook;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.49 no.1
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    • pp.35-38
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    • 2009
  • The zebra shark Stegostoma fasciatum which had been reared in the commercial aquaria was found dead and submitted for postmortem examination. A pure bacterial culture was isolated from pale and enlarged liver. The analysis of ureC and 16S rRNA genes confirmed the isolate as Photobacterium (P.) damselae subsp. damselae and this pathogen was sensitive to gentamicin. Although, no mortality in mouse was observed in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark.

PRELIMINARY X-Ray DIFFRACTION STUDY OF Pseudomonas sp. DJ77 GLUTATHIONE S-TRANSFERASE

  • Park, Heung-Soo;Chung, An-Sik;Ryu, Seong-Eon;Suh, Se-Won;Kim, Young-Chang;Chung, Yong-Je
    • Proceedings of the Korean Biophysical Society Conference
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    • 1996.07a
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    • pp.20-20
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    • 1996
  • Crystals of a bacterial glutathione S-transferase(pGST) from pseudomonas sp. DJ 77 have been grown by hanging drop method of vapour diffusion from ammonium sulfate solution. The low concentration of polyethylene glycol 400 as additive were found to be essential for the reproducible growth of large single crystal of pGST. (omitted)

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An X-ray Crystallographic Analysis of UDP-N-Acetylglucosamine Enolpyruvyl Transferase from Haemophilus influenzae in Complex with UDP-N-Acetylglucosamine and Fosfomycin

  • Yoon, Hye-Jin;Ku, Min-Je;Ahn, Hyung-Jun;Kim, Hyung-wook;Suh, Se-Won
    • Proceedings of the Korean Biophysical Society Conference
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    • 2002.06b
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    • pp.28-28
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    • 2002
  • Peptidoglycan is an extensively cross-linked polymer essential for the integrity of the bacterial cell wall. Many antibiotics act by disruption of its biosynthesis and assembly, several are targeted against the cytoplasmic enzymes that synthesize the key intermediate UDP-N-acetylmuramyl pentapeptide. One such drug is fosfomycin, which inactivates the first enzymes in this pathway, UDP-N-acetylglucosamine enopyruvyl transferase (murZ).(omitted)

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