• The Korean Journal of Food And Nutrition
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• v.14 no.4
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• pp.333-338
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• 2001

In research. which Korean pickled cucumber was treated wish various methods, including blanching. brining in hot solution, and treating with trehalose. I examined the changes of properties of material. The Korean pickled cucumber were fermented 4∼5$^{\circ}C$ for 42 days in 1% salt solution. The physiochemical properties were pH, total acidity, total cell count, lactic acid bacteria and texture properties were also evaluated. The result showed that the effect of blanching and soaking cucumber in 100$^{\circ}C$ hot salt solution significantly reduced the softening rate of texture while a rather rapid fermentation was found for those preserved with salt. The effect of trehalose treatment inhenced fermentation but it was significantly reduced softening rate of texture. The texture evaluation of Korean pickled cucumber was found that heat treatment with blanching after soaked in hot solution and trehalose treatment had a positive effect for reduction of softening of cucumber tissue.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.808-811
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• 2001

The rheological properties of exopolysaccharide(EPS-R) produced by marine bacteria Hahella chjuensis KCTC 2395 was investigated. EPS-R solution showed a characteristic non-Newtonian behavior fluid properties. In aqueose dispersions of EPS-R 1%, consistency index(K) and flow behavior index(n) were 1,410 cp and 0.73. EPS-R solution was pseudoplastic fluid by power-low model. Rheological propertie of EPS-R was found to be influenced by the concentration of salt, pH, temperature and ionic compounds.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.796-797
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• 2001

The $Ag^{2+}$ solution was constructed by supplying pure metal silver in filtrated distilled with constant voltage. The solution at the $Ag^{2+}$ concentration of 10 ppm showed specific activity against Gram positive and negative bacteria. More than 90% activity showed against Candida albicans ATCC 102321 at the same concentration. The addition of DASA, $Ag^{2+}$ and artemisia mixture, in the EVLN had same activity by comparison with chemical synthetic antiseptic.

• Journal of Soil and Groundwater Environment
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• v.17 no.4
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• pp.9-18
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• 2012

This study was carried out to leach valuable metal ions from the mine waste ore using the adapted indigenous bacteria. In order to tolerance the heavy metals, the indigenous bacteria were repeatedly subcultured in the adaptation-medium containing $CuSO_4{\cdot}5H_2O$ for 3 weeks and 6 weeks, respectively. As the adaptation experiment processed, the pH was rapidly decrease in the adaptation-medium of 6 weeks more than the 3 weeks. The result of bioleaching with the adapted bacteria for 42 days, the pH value of leaching-medium in the 3 weeks tend to increased, whereas the pH of the 6 weeks decreased. In decreasing the pH value in the adaptation-medium and in the leaching-medium, it was identified that the indigenous bacteria were adapted $Cu^{2+}$ the ion and the mine waste ores. The contents of Cu, Fe and Zn in the leaching solution were usually higher leached in 6 weeks than 3 weeks due to the adaptation. Considering the bioleaching rates of Cu, Fe and Zn from these leaching solutions, the highest increasing the efficiency metal ion were found to be Fe. Accordingly, it is expected that the more valuable element ions can be leached out from the any mine waste, if the adapted bacteria with heavy metals will apply in future bioleaching experiments.

• Food Science and Preservation
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• v.16 no.5
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• pp.605-611
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• 2009

Korean Baechu cabbage(known as Chinese cabbage) brined in 0%, 5% and 10% (all w/v) salt solutions were packed using high-density polyethylene film (HDPE-film), low-density polyethylene film (LDPE-film), or Mirafresh film (MF-film, US patent No. 5972815), and stored at 4C for 4 weeks. Changes in pH and salinity, and microorganism counts (lactic acid bacteria and total bacteria), were investigated. There was no significant difference in the pH change in cabbage stored using various films when the vegetables were not salted. However, the pH was most stable in Baechu cabbage prepared using 10% salt solution. Cabbage treated with 0%, 5%, and 10% salt showed salinity values of 0.83%, 1.17% and 1.62%(all w/w), respectively, after 4 weeks of storage by LDPE-film. When cabbage was treated with the highest concentration of salt solution, the count of lactic acid bacteria increased but that of total bacteria decreased. The pH from pH 6.10 to pH 4.32, pH 5.68, and pH 5.92 in brined cabbage packed in HDPE-film, LDPE-film, and MF-film, respectively, after 4 weeks. When MF-film was used, the pH showed the greatest stability of all films tested, regardless of the concentration of salt solution employed in brining. The counts of lactic acid bacteria and total bacteria increased by all tested films during storage. Cabbage packed by MF-film showed the lowest increase in bacterial counts. In conclusion, MF-film was found to be the most effective packaging material for Baechu cabbage and brining in 10% salt solution was optimal to enhance the shelf life of the vegetable. LDPE-film was more effective than was HDPE-film for storage of brined cabbage.

• International Journal of Oral Biology
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• v.38 no.4
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• pp.149-154
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• 2013

In general, oleanolic acid (OA) and ursolic acid (UA) have antimicrobial effect against Gram-positive bacteria but not Gram-negative bacteria whereas sophoraflavanone G has antimicrobial activity against both bacterial types. However, the antimicrobial effects of OA, UA, and sophoraflavanone G against periodontopathogens have not been studied to any great extent. The aim of this study was to investigate antimicrobial effect of OA, UA, and sophoraflavanone G against 15 strains (5 species) of oral Gram-negative bacteria, which are the major causative bacteria of periodontal disease. The antimicrobial activity was evaluated by minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determinations. OA and UA showed antimicrobial effects against all of the Porphyromonas gingivalis strains tested and also Prevotella intermedia ATCC $25611^T$. Interestingly, P. intermedia ATCC 49046 showed greater resistance to OA and UA than P. intermedia ATCC $25611^T$. In contrast, sophoraflavanone G had antimicrobial activity against all strains, with MIC and MBC values below $32{\mu}g/ml$, except Aggregatibacter actinomycetemcomitans. These results indicate that sophoraflavanone G may have potential for use in future oral hygiene products such as dentifrices and gargling solution to prevent periodontitis.

• Journal of Environmental Health Sciences
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• v.32 no.1 s.88
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• pp.77-88
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• 2006

This study were conducted to investigate the microbial contamination level in 5 tomato farms in Western Gyeongnam. A total of 130 samples was examined for sanitary indicator bacteria, such as aerobic plate count (APC), coliforms, and Escherchia coli, and pathogenic bacteria such as E. coli O157:H7, Salmonella spp., Staphylococcus aureus, and Listeria monocytogenes. APC and coliform count ranged $0\~6.62\;and\;0\~4.52 log_{10}\;CFU/(ml,\;g,\;100\;cm^2,\;hand)$, respectively, and $32.5\%$ were contaminated with E. coli. Especially, most of the samples from employees are high as above $4.0\;log_{10}\;CFU/(ml,\;g,\;100\;cm^2,\;hand)$ in APC. S. aureus, detected at $10.7\%$, was found in employees' hands, irrigation water, and hydroponic solution. whereas E. coli $O157:H7$, Salmonella spp, and L. monocytogenes were not detected. These results will provide fundamental microbiological information for introduction of good agricultural practice (GAP)system in tomato farms.

• Fisheries and Aquatic Sciences
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• v.5 no.3
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• pp.150-155
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• 2002

Marine bacteria producing algicidal substance against Cochlodinium polykrikoides was screened and isolated from seawater. Metabolite of marine bacteria Micrococcus sp. LG-5 showed strong algicidal activity against C. polykrikoides. C. polykrikoides was inhibited above $90\%$ at $5\%$ solution of the metabolite within 24 hrs. Algicidal substance from the metabolite was extracted with ethyl acetate, and then purified by PTLC and reversed-phase HPLC. Algicidal activity of purified compound against C. polykrikoides was above $90\%$ at 3.7, 11.0 and 33.0${\mu}g/mL$ concentration after 12, 9 and 3 hrs, respectively. Ninety percent inhibition of other red tides, Gymnodinium sanguineum and Gyrodinium impudicum was observed when treated with 3.7${\mu}g/mL$ of purified compound within a period of 12 hrs. The microscopic view of red tides treated with purified compound showed the deformations such as cell node cuts and swelling of cells.

• Journal of the Mineralogical Society of Korea
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• v.27 no.4
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• pp.207-222
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• 2014

In order to recover the metallic copper powder from the mine-waste rock, heap bioleaching, Fe removal and electrowinning experiments were carried out. The results of heap leaching with the mine-waste rock sample containing 0.034% Cu showed that, the leaching rate of Cu were 61% and 62% in the bacteria leaching and sulfuric acid leaching solution, respectively. Sodium hydroxide (NaOH), hydrogen peroxide ($H_2O_2$) and calcium hydroxide ($Ca(OH)_2$) were applied to effectively remov Fe from the heap leaching solution, and then $H_2O_2$ was selected for the most effective removing Fe agent. In order to prepare the electrolytic solution, $H_2O_2$ were again treated in the heap leaching, and Fe removal rates were 99% and 60%, whereas Cu removal rates were 5% and 7% in the bacteria and sulfuric acid leaching solutions, respectively. After electrowinning was examined in these leaching solution, the recovery rates of Cu were obtained 98% in bacteria and obtained 76% in the sulfuric leaching solution. The dendritic form of metallic copper powder was recovered in both leaching solutions.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1115-1120
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• 2002

The effects of seed-associated or free linseed oil on fermentation characteristics and long-chain unsaturated fatty acids composition, especially the formation of conjugated linoleic acid (CLA) and octadecenoic acid (trans-11 $C_{18:1}$, $t-C_{18:1}$) by mixed ruminal bacteria were examined in vitro. Concentrate (1% of culture solution, w/v, as-fed basis) with ground linseed (0.6% of culture solution, w/v, DM basis) or linseed oil as absorbed onto ground alfalfa hay was added to 600 ml mixed solution consisting of strained rumen fluid and artificial saliva at the ratio of 1:1 in a glass culture jar. The culture jar was covered with a glass lid with stirrer, and placed into a water-bath ($39^{\circ}C$) and incubated anaerobically up to 24 h. Seed-associated or free linseed oil did not significantly affect the pH and ammonia concentration in the culture solution. Molar percent of acetate tended to increase while that of propionate decreased with the addition of free oil treatment throughout the incubation. Differences in bacterial number were relatively small, regardless of the form of supplements. Decreasing trends in the compositions of linoleic acid ($C_{18:2}$) and linolenic acid ($C_{18:3}$) but increasing trends of stearic acid ($C_{18:0}$), $t-C_{18:1}$ and CLA compositions were found from culture contents up to 12h incubation when incubated with both ground linseed and linseed oil. The compositions of $C_{18:0}$, $C_{18:2}$ and $C_{18:3}$ were greater but those of oleic acid ($C_{18:1}$), $t-C_{18:1}$ and CLA were smaller in a culture solution containing ground linseed than those containing linseed oil. The ratio of $t-C_{18:1}$ to CLA was lower in the culture solutions containing linseed oil up to 12h incubations as compared to those containing ground linseed.