• Parasites, Hosts and Diseases
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• v.33 no.3
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• pp.155-164
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• 1995

The location of actin and myosin of the several stages of Cwptosporinium parvum was observed. The tissue antigen of C. pcruum was prepared through immunosuppression of IgG mice with Depomedrol . The thin sectioned specimens, which were incubated with the IgG fraction of the rabbit polyclonal antibodies raised against chicken back muscle actin and bovine uterus myosin, were treated with 10 nm gold-conjugated goat anti-rabbit IgG, Electrodense particles were located mainly on the pellicles of all observed developmental stages of the parasites. The number of actin gold particles in the cytoplasm increased when the parasite was dividing actively as in case of meronts. Especially in macrogametocytes, a lot of actin and myosin particles were synthesized and storaged as amilopectin-like bodies. There were many actin gold particles along the microspikes of cytoplasmic membranes in various developmental stages. The actin and myosin observed in this study may play important roles to control the shape of the parasites and movement of cytoplasmic membranes as cvtoskeletal proteins.

• Journal of International Academy of Physical Therapy Research
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• v.10 no.4
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• pp.1897-1902
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• 2019

Background: Tension-type headaches, which make up the highest proportion of headaches, are prone to develop into chronic tension-type headaches (CTTH). The characteristic of CTTH in patients is that the active myofascial trigger point (ATrP) which causes pain in the muscles of the back of the head is increased, compared to the normal headache and moves the head position forward. Objective: The aim of this study was to investigate the effects of myofascial release (MFR) and posture correction in effectively improving neck function and sleep quality in the symptoms of CTTH patients. Design: Observer-blind study Methods: To reduce ATrP, MFR was applied and exercise was also applied to correct posture. The subjects of this study were 48 individuals randomly divided into three groups; The MFR group using the MFR technique; The MFR with exercise group subject to both the MFR technique and forward head position correction exercises (MFREx), and the control group. MFR and MFREx groups were given the relevant interventions twice a week for four consecutive weeks, and went through the number ATrPs, range of motion (ROM) of neck, Neck Disability Index (NDI) and the Pittsburgh Sleep Quality Index (PSQI) before and after the intervention. A physical therapist, who was fully familiar with the measuring methods of the equipment, was the measurer and not aware of the target's condition was blinded to take measurements only before and after intervention. Results: There was a significant improvement in the ATrP, Neck ROM, NDI and PSQI in the group of patients to whom the MFR technique and MFREx were applied. MFREx was more effective in increasing neck mobility. Conclusions: According to this study, the application of MFR is effective in improving neck movement and sleep quality in chronic tension headache patients.

• Food Science of Animal Resources
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• v.39 no.3
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• pp.388-401
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• 2019

This study was conducted to investigate the effect of the total digestible nutrients (TDN) level of commercial concentrates on growth performance, carcass characteristics, and meat composition of late fattening Hanwoo steers. A total of 28 steers were randomly assigned to one of four dietary groups; T1 (73.30% TDN), T2 (74.50% TDN), T3 (76.40% TDN), and T4 (77.10% TDN). Average daily gain (ADG) was slightly but not significantly higher in the T2 than in the other treatments. Dry matter intake (DMI) and feed conversion ratio (FCR) were higher in the T2 than in the other treatments; however, the differences were not statistically significant. Carcass back fat thickness was thicker in the T4 and marbling score was higher in the T2 than in the other treatments; however, the differences were not statistically significant. The TDN level of concentrates had no effect on the physicochemical characteristics and fatty acid composition of the longissimus muscle. The finding of this study indicate that less than 74% or greater than 75% TDN in the commercial concentrate did not contribute to improve ADG, FCR, marbling score; therefore, in the present study, the recommendable TDN level in the commercial concentrate for late fattening period was 74% to 75% in terms of growth performance and marbling score of Hanwoo steer.

• Korean Journal of Agricultural Science
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• v.46 no.2
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• pp.293-301
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• 2019

This study was done to estimate the effect of progeny numbers and pedigree depth on the accuracy of the estimated breeding value (EBV) using best linear unbiased prediction (BLUP) method in Hanwoo. The experiment groups (sire = 100, 200, and 300; progeny = 4 and 8) were made by random sampling and by genetic evaluation of the following traits: Body weight (BW), carcass weight (CW), eye muscle area (EMA), back fat thickness (BFT) and marbling score (MS9). As a result of the genetic evaluation, the accuracy of the EBV was roughly 30 - 60% with 4 progenies, and the accuracy of the EBV increased by about 50 - 75% with 8 progenies. In the other words, when the number of progenies increased from 4 to 8, the accuracy of the EBV simultaneously increased by about 15 - 20%. Moreover, when the number of sires was higher, variations in the accuracy of the EBV within the groups for each trait decreased. Therefore, this result indicates that not only the number of progeny but also the number of sires can affect the accuracy of the EBV. Consequently, collecting information on the progeny and careful management of that information are very important things in the Hanwoo breeding system. Therefore, the EBV can show more precise results when conducting genetic evaluations.

• Journal of Acupuncture Research
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• v.38 no.2
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• pp.170-174
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• 2021

A patient diagnosed with Guillain-Barre syndrome (GBS) suffering from numbness in the toes and fingers, paralysis in the face, and back pain received intravenous immunoglobulin on December 28, 2019. Without an improvement in symptoms the patient was admitted to a Korean medicine hospital on January 1, 2020 to receive combined Korean medicine treatment including herbal medicine (Sipjeondaebo-tanggami and Hyangbujasunkipalmul-tang), acupuncture treatment, moxibustion, cupping treatment, and physical therapy. Treatment was administered over 51 days, of which 17 days were during hospitalization. Paralysis of facial muscle, and numbness in fingers and toes were evaluated using the Yanagihara score and numeric rating scale (NRS) score, respectively. Facial paralysis improved (21 to 40 based on Yanagihara score) and paresthesia of the hands and feet disappeared (NRS score 6-7 to NRS score 0). There were no side effects or sequela. This study suggested combined Korean medicine treatment may be effective for the treatment of GBS.

• Archives of Plastic Surgery
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• v.48 no.1
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• pp.33-43
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• 2021

Background Acellular dermal matrices (ADMs) have become an essential material for implant-based breast reconstruction. No previous studies have evaluated the effects of sterility of ADM under conditions of radiation. This study compared sterile (irradiated) and aseptic (non-irradiated) ADMs to determine which would better endure radiotherapy. Methods Eighteen male Balb/C mice were assigned to the control group with no irradiation (group 1) or one of two other groups with a radiation intensity of 10 Gy (group 2) or 20 Gy (group 3). Both sterile and aseptic ADMs were inserted into the back of each mouse. The residual volume of the ADM (measured using three-dimensional photography), cell incorporation, α-smooth muscle actin expression, and connective tissue growth factor expression were evaluated. The thickness and CD3 expression of the skin were measured 4 and 8 weeks after radiation. Results In groups 2 and 3, irradiated ADMs had a significantly larger residual volume than the non-irradiated ADMs after 8 weeks (P<0.05). No significant differences were found in cell incorporation and the amount of fibrosis between irradiated and non-irradiated ADMs. The skin was significantly thicker in the non-irradiated ADMs than in the irradiated ADMs in group 3 (P<0.05). CD3 staining showed significantly fewer inflammatory cells in the skin of irradiated ADMs than in non-irradiated ADMs in all three groups after 4 and 8 weeks (P<0.05). Conclusions Under radiation exposure, irradiated ADMs were more durable, with less volume decrease and less deposition of collagen fibers and inflammatory reactions in the skin than in non-irradiated ADMs.

• PNF and Movement
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• v.19 no.3
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• pp.375-382
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• 2021

Purpose: The objectives of the present study were to investigate the disadvantages of tire pressure asymmetry of a wheelchair tire and recommend the criterion for appropriate tire pressure without generating negative changes in the musculoskeletal system in asymptomatic participants. Methods: Fourteen asymptomatic participants were asked to sit in pressure-controlled wheelchairs and perform desk work for 20 minutes in each tire condition. The asymmetry of the tire conditions was set as 0% difference, 25% difference, and 50% difference from the recommended pressure. The pelvic alignment and muscular recruitment represented as a flexion-relaxation ratio (FRR) were measured at pre-test, and after each condition of desk work. The displacement of the center of pressure (COP) was measured during the desk work. Results: The tire air pressure condition significantly affected the FRR and COP (P < 0.05). Both sides of the FRR values were significantly higher under the symmetrical tire conditions (0% difference) and pre-test, compared with the asymmetrical tire condition of 50% difference (P < 0.05). The mediolateral COP displacement of the asymmetrical tire conditions (25% and 50% difference) was significantly higher than that of the symmetrical tire conditions (0%) (P < 0.05). Conclusion: Asymmetrical tire conditions could cause changes in the muscle recruitment pattern of the erector spine and mediolateral COP displacement. Tire pressure asymmetry higher than 50% could be a risk factor for prevalence of back pain, so this level of asymmetry in tire pressures should be cautioned against for wheelchair users.

• PNF and Movement
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• v.20 no.3
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• pp.431-441
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• 2022

Purpose: The purpose of this study was to compare the changes in the contact area, maximum pressure, maximum mean pressure, and maximum force of functional insoles and general insoles when walking. Methods: Foot pressure was measured by the ignition of functional insoles and general insoles on Company N shoes. The foot pressure was measured using a precision pressure distribution meter (Pedar - X mobile system, Novel, Germany). Each insole sensor contained 99 independent cells and was inserted between the foot and the shoe. A wireless Bluetooth-type program was used to measure the pressure detected by the measuring insoles. In order to eliminate adaptation and fatigue caused by wearing the guide during the experiment, sufficient rest was taken between each experiment, and the wearing order was randomly selected. Results: Functional insole significantly increased the forefoot and midfoot (medial, lateral) (p<0.05), while total foot, forefoot, and rearfoot peak pressure significantly decreased (p < 0.05) compared to the general insole. Conclusion: In the functional insole, a high contact area was measured inside, even in the middle of the foot, leading to a proper change in foot pressure. It was confirmed that the contact area was reduced and dispersion occurred well. In addition, it was found that the maximum pressure in the front and back of the entire foot was reduced, so the weight pressure dispersion in the functional insole was evenly distributed, and the maximum average pressure change was similar.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.1
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• pp.32-37
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• 2023

This study has evaluated the genomic estimated breeding value (GEBV) of the commercial Hanwoo population using the genomic best linear unbiased prediction (GBLUP) method and genomic information. Furthermore, it analyzed the accuracy and realized accuracy of the GEBV. 1,740 heads of the Hanwoo population which were analyzed using a single nucleotide polymorphism (SNP) Chip has selected as the test population. For carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), and marbling score (MS), the mean GEBVs estimated using the GBLUP method were 3.819, 0.740, -0.248, and 0.041, respectively and the accuracy of each trait was 0.743, 0.728, 0.737, and 0.765, respectively. The accuracy of the breeding value was affected by heritability. The accuracy was estimated to be low in EMA with low heritability and high in MS with high heritability. Realized accuracy values of 0.522, 0.404, 0.444, and 0.539 for CWT, EMA, BFT, and MS, respectively, showing the same pattern as the accuracy value. The results of this study suggest that the breeding value of each individual can be estimated with higher accuracy by estimating the GEBV using the genomic information of 18,499 reference populations. If this method is used and applied to individual selection in a commercial Hanwoo population, more precise and economical individual selection is possible. In addition, continuous verification of the GBLUP model and establishment of a reference population suitable for commercial Hanwoo populations in Korea will enable a more accurate evaluation of individuals.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.681-691
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• 2021

The estimated breeding value (EBV) and accuracy of Hanwoo steer (Korean cattle) is an indicator that can predict the slaughter time in the future and carcass performance outcomes. Recently, studies using pedigrees and genotypes are being actively conducted to improve the accuracy of the EBV. In this study, the pedigree and genotype of 46 steers obtained from livestock farm A in Gyeongnam were used for a pedigree best linear unbiased prediction (PBLUP) and a genomic best linear unbiased prediction (GBLUP) to estimate and analyze the breeding value and accuracy of the carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS). PBLUP estimated the EBV and accuracy by constructing a numeric relationship matrix (NRM) from the 46 steers and reference population I (545,483 heads) with the pedigree and phenotype. GBLUP estimated genomic EBV (GEBV) and accuracy by constructing a genomic relationship matrix (GRM) from the 46 steers and reference population II (16,972 heads) with the genotype and phenotype. As a result, in the order of CWT, EMA, BFT, and MS, the accuracy levels of PBLUP were 0.531, 0.519, 0.524 and 0.530, while the accuracy outcomes of GBLUP were 0.799, 0.779, 0.768, and 0.810. The accuracy estimated by GBLUP was 50.1 - 53.1% higher than that estimated by PBLUP. GEBV estimated with the genotype is expected to show higher accuracy than the EBV calculated using only the pedigree and is thus expected to be used as basic data for genomic selection in the future.