• Title/Summary/Keyword: Bacillus thuringiensig subsp. israelensis

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Isolation of Oligosporogenous (Spo-Cry+)Mutant Containing Relatively tow Hemolytic Activity in $\delta$-Endotoxin from Bacillus thuringiensig subsp, israelensis (Bacillus thuringiensig subsp, israelensis의 용혈성 약화 및 무포자 변이주의 분리)

  • 배점순;김광현
    • Microbiology and Biotechnology Letters
    • /
    • v.17 no.4
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    • pp.273-276
    • /
    • 1989

  • The isolate SPO 3, an oligosporogenous and crystalliferous mutant, which was isolated from Bacillus thuringiensig subsp, israelensis by heat treatment at 42$^{\circ}C$ for 24 hit. The $\delta$-endotoxin of the mutant had a relatively low hemolytic activity on human red blood cells; the $\delta$-endotoxin of the mutant had 25 times less hemolytic activity compared to that of wild strain. The loss of 28 KDa hemolytic protein subunit in $\delta$-endotoxin of the mutant was confirmed by means of double immunodiffusiori, immunoelectrophoresis, and SDS-PAGE.

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