• BMB Reports
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• 제33권4호
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• pp.332-336
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• 2000

Phenazine 1-carboxylic acid (PCA) is an antifungal antibiotic isolated from a culture filtrate of Bacillus sp. B-6 producing an acyl CoA synthetase inhibitor. This antibiotic is reported as an inhibitor of an acyl CoA synthetase from Pseudomonas sp.. Bacillus sp. B-6 was resistant to PCA up to 350 ${\mu}g/ml$. We investigated the mechanism of the resistance of Bacillus sp. B-6 to PCA. The rate of growth in a medium containing up to 100 ${\mu}g/ml$ was as rapid as the PCA-free medium. At a PCA concentration of 300 ${\mu}g/ml$, the growth rate was more than half that of the control. In this work, we purified acyl CoA synthetase from Bacillus sp. B-6 and found that this acyl CoA synthetase was much less sensitive to PCA than the acyl CoA synthetase from other source. These findings suggested that the insensitivity of Bacillus sp. B-6 acyl CoA synthetase plays an important role in the PCA resistance of this bacterium.

• 약학회지
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• 제42권6호
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• pp.552-557
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• 1998

Acyl CoA synthetase inhibitor, was purified from the culture broth of a Bacillus sp. B-6, which had been isolated from soil, by chloroform extract, silica gel column chro matography and preparative TLC. The purified acyl CoA synthetase inhibitor showed higher Antifungal activity against C. al-bicans (MIC: 8${\mu}$g/ml). Though the analysis of UV spectrum, melting point, IR spectrum, Mass-spectrum, $^1H$-NMR and $^{13}C$-NMR spectrum, the inhibitor could be identified as phenazine-l-carboxylic acid.