• 한국미생물·생명공학회지
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• 제39권1호
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• pp.49-55
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• 2011

토양으로부터 분리한 Streptomyces sp. 30여 균주들과 한국전통식품에서 분리한 Bacillus sp. 200여 균주들로부터 ${\alpha}$-glucosidase 의 활성을 저해하고 동시에 DNJ를 생산하는 유용균주를 선발하였다. 실험결과 한국전통식품인 청국장으로부터 ${\alpha}$-glucosidase 저해능이 높고 DNJ 생산능이 우수한 한개의 균주를 선발하였다. 이 균주의 동정을 위하여 API kit에 의한 균의 당 이용능 분석, HPLC와 GC에 의한 균체의 quinone 및 지방산 분석 등과 함께 균의 16S rDNA 염기서열을 분석하였으며 주사전자현미경에 의해 균주의 형태적 특성을 관찰하였다. 그 결과 본 연구를 통해 선발한 균주는 건강기능성식품 개발 등에 적용할 수 있는 GRAS에 속하는 균주임을 확인하여 B. subtilis MORI로 명명하였다.

• Journal of Microbiology and Biotechnology
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• 제20권8호
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• pp.1210-1214
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• 2010

Many bacteria are involved in the fermentation of doenjang, and Bacillus species are known to perform significant roles. Although SDS-PAGE has been frequently used to classify and identify bacteria in various samples, the microbial diversity in doenjang has not yet been investigated. This study aims to determine the identity and distribution of dominant Bacillus species in doenjang using SDS-PAGE profiles of whole-cell proteins and 16S rRNA gene sequencing. Reference Bacillus strains yielded differential SDS-PAGE banding patterns that could be considered to be highly specific fingerprints. Grouping of bacterial strains isolated from doenjang samples by whole-cell protein patterns was confirmed by analysis of their 16S rRNA gene sequences. B. subtilis was found to be the most dominant strain in most of the samples, whereas B. licheniformis and B. amyloliquefaciens were less frequently found but were also detected in several samples. The results obtained in this study show that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully identify Bacillus species isolated from doenjang.

• 산업식품공학
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• 제14권3호
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• pp.263-270
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• 2010

Xylanase를 생산하는 호알칼리성 균주를 토양으로부터 분리하여 동정하였다. 토양으로부터 분리한 호알칼리성 균주 3000여종 중 xylanase 활성이 가장 높은 균주인 strain DK-2386 균주를 선별하였다. Gram 염색과 SEM을 통한 형태학적 특성을 관찰하였으며, Methods for General and Molecular Bacteriology와 Bergey's manual of systematic bacteriology, 그리고 Biochemical tests for identification of medical bacteria에 준하여 생화학적 특성을 확인한 결과 배양액의 색은 붉은 빛을 나타내었으며, Gram양성 간균, 내생포자형성, catalase 양성, oxidase 양성, 혐기성 양성, glucose로부터 gas 형성 양성, casein 가수분해 양성, starch 가수분해 양성, CMC 가수분해 양성, xylan 가수분해 양성, nitrate 환원능 양성이며 10% NaCl 농도에서도 생육하는 것을 확인하였다. 균체의 지방산 조성 분석 결과 $C_{15:0\;ISO}$ 27.35%와 $C_{15:0\;ANTEISO}$ 31.54%, $C_{16:0}$ 9.70%로 이루어져 Bacillus속으로 동정되었으며, 16S rDNA 염기서열 분석결과 B. agaradhaerens와 100%의 유사성을 갖는 것을 확인하였다. (사)한국종균협회로부터 분양받은 Bacillus agaradhaerens 40952, B. alcalophilus와 strain DK-2386 균주사이의 생화학적 특성을 비교한 결과 B. agaradhaerens와 속과 종이 같으나 미세하게 특성이 다른 균주로 판단되어 B. agarad haerens DK-2386이라 명명하였다.

• 미생물학회지
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• 제1권1호
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• pp.30-37
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• 1963

Ten(10) strains of aerobic bacteria and two(2) strains of microaerobic bacteria were isolated out of soysauce which was brewed by the conventional process. The following bacteria were identified by studying their morphorogies and physiological characters. Bacillus pumillus-R-2; Bacillus subtilis var., aterimus-S-1; Bacillus licheriformis var-S-2; Bacillus subtilis-T-1; Sarcina maxima-T-2; Pediococcus acidi lactici-Z-2; Bacillus citreus var, . soyaB-Z-5. T-2 and Z-5 of the isolated bacteria were found good in growth even in the 24%-salted density, and Z-5 was more vigorous than T-2 though stinking. S-1 produced black-brown pigment from the medium containing various kinds of carbohydrate and the medium of soysauce which are available to S-1.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1111-1114
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• 2001

An extracellular fibrinolytic-enzyme-producing bacterium was isolated from Doen-Jang, a Korean traditional fermented flood, and identified as Bacillus sp. DJ based on its morphology and cellular fatty acid composition. The total extracellular fibrinase (EF) from Bacillus sp. DJ was analyzed using three fibrin zymographic techniques, SDS-fibrin zymography (SDS-FZ), isoelectrofocucing-fibrin zymographs(IEF-FZ), and a two-dimensional SDS-fibrin zymographic analysis (2D SDS-FZ). As a result, the EP map of Bacillus sp. DJ was established. The results suggest that the 2D SDS-FZ method will be a useful tool for the proteomic approach for many other bacterial pretenses.

• 한국식품영양학회지
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• 제11권6호
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• pp.622-628
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• 1998

Microorganisms isolated from soil were tested for their flocculating activity in kaolin suspension, Identification of the best producing CH-23 strain showed that the strain belonged to the Bacillus megaterium. The maximum production of the flocculating from Bacillus megaterium CH-23 was observed in the culture medium containing 2% sucrose, 3% NaNo3, 0.1% K2HPO4, 0.5% NaCl, 0.5% MgSO4.7H2O and 0.01% tryptone at initial pH 7.0 and 25~3$0^{\circ}C$. Flocculating activity was improved to 57% when the culture medium contained Mn2+(0.01% MnSo4). In the culture medium containing Mg2+(0.01% MgSO4.7H2O) and Ca2+(0.01% CaCO3), flocculating activity were reached to 48% and 33%, respectively.

• 한국환경보건학회지
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• 제12권2호
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• pp.67-74
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• 1986

The study was carried out to investigate for the property of Bacillus strains, on the native growthed microflora in Korean native soybean paste, and Bacillus strains of the high enzyme producing, were selected and identificated, from the microflora, that is, identificated Bacillus strains beared resemblance to B. subtills, on the colony, appearance was pellicle, surface's spreading, color creamy-thin browned, colony elevation flated, and edge lobated, the identfficated B. subtills strain named for the B. subtilis SCF. For the protease activity of B. subtilis SCF, according to the variation with pH, the pH stability was pH 7~8, and on the its protease activity, optimum temperature was 40$\circ$C, on the other hand, temperature of the highest stability of the protease was 50$\circ$C.

• 한국미생물·생명공학회지
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• 제21권5호
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• pp.486-493
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• 1993

A gram(+) bacteria that produced microbial flocculant was isolated from soil and classified as a Bacillus species and named as Bacillus sp. A56. The culture conditions of the strain for fluocculant production were studied in a shake flask. Optimum temperature and initial pH for flcculant production were 30C and 6.5, respectively. The optimized medium has gollowing composition: glucose 20g/l, NH4NO3 0.5g/l, K2HPO4 1.0g/l, KH2PO4 0.8g/l, MgSO4.7H2O 0.2g/l, MnSO4.4-6H2O 0.3g/l, CaCO3 0.5g/l, yeast extract 0.3g/l, tryptone 0.3g/l in tap water.

• 대한미생물학회지
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• 제35권1호
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• pp.31-40
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• 2000

Bacillus anthracis, the etiological agent of anthrax has been classified into the Bacillus subgroup I with B. cereus, B. mycoides and B. thuringiensis based on morphological and DNA similarity. DNA studies have further indicated that these species have very AT-rich genomes and high homology, indeed it has been proposed that these four sub-species be recognized as members of the one species. Several methods have been developed to obtain good differentiation between these species. However, none of these methods provides the means for an absolutely correct differntiation. The analysis of fatty acid methyl esters (FAMEs) was employed as a quick, simple and reliable method for the identification of 21 B. anthracis strains and closley related strains. The most significant differences were found between B. anthracis and B. anthracis closely related strains in FAMEs profiles. All tested strains of B. anthracis had a branched fatty acid C17:1 Anteiso A, whereas the fraction of unsaturated fatty acid Iso C17:1 w10c was found in B. anthracis closely related strains. By UPGMA clustering analysis of FAMEs profiles, all of the tested strains were classified into two clusters defined at Euclidian distance value of 24.5. The tested strains of B. anthracis were clustered together including Bacillus sp. Kyungjoo 3. However, the isolates of B. anthracis closely related spp. Rho, S10A, 11R1, CAU9910, CAU9911, CAU9912 and CAU9913 were clustered with the other group. On the basis of these results, isolates of B. anthracis Bongchon, Kyungjoo 1, 2 and Bacillus sp. Kyungjoo 3 were reclassified as a B. anthracis. It is concluded that FAMEs analysis provides a sensitive and reliable method for the identification of B. anthracis from closely related taxa.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.359-363
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• 2019

국내 5개 지역에서 수집한 12개 메주로부터 Bacillus, Enterococcus, Staphylococcus 속 bacteria를 선택배지를 이용하여 분리하고, 16S ribosomal RNA 유전자 및 gmk(guanylate kinase) 유전자 염기서열분석을 통해 동정하여, 이들 속의 우점종을 검토하였다. Bacillus 속과 Enterococcus 속은 모든 시료로부터 검출되었고, Bacillus 속은 11개 시료에서 총균수 대비 15% 이상 검출되어 메주 발효에서 가장 우점하는 bacteria로 확인되었다. Staphylococcus 속은 6개 시료에서만 검출되었다. Bacillus 속으로 동정된 151개 분리주는 B. velezensis, B. sonorensis 순으로 우점하였고, B. subtilis, B. licheniformis가 그 뒤를 이었다. Enterococcus 속으로 동정된 165개 분리주 중, 163균주가 E. faecium으로 확인되었다. Staphylococcus 속으로 동정된 82개 분리주에는 6종의 coagulase-negative Staphylococcus 존재가 확인되었고, S. xylosus가 우점종으로 확인되었다.