• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.369-374
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• 1997

Immature flower buds of 'Desio' carnation were cultured on MS agar medium supplemented with 1 ㎎/L 2,L-D. Embryogenic calli were formed from 5-10% of the buds less than 20 ㎜ in length, but only non-embryogenic calli were produced from explants of shoot apex leaf, internode, and flowere buds larger than 20 ㎜. The same method was applied to 16 cultivars of cut Sower carnation and embryogenic calli were obtained in 7 cultivars. Several embryogenic callus lines were selected and maintained through subcultures over 120 weeks without loss of embryogenic competence. The embryogenic cultures were also proliferated rapidly in liquid agitation cultures using MS medium supplemented with 1mg/L 2,4-D. Numerous embryos were formed on the periphery of the cell aggregates upon transfer to auxin-free MS agar medium. Plantlets were transplanted in potting soil and grown to bloom in six months.

• Mycobiology
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• v.28 no.1
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• pp.54-56
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• 2000

A black scab disease occurred on sword bean (Canavalia gladiata) in plastic film houses around Chinju area during the spring season of 1999. The disease started from flower bud, then moved to flower stalk, pod, petiole, cirrus, stem and leaves. The lesions started with small dark brown spots then were gradually expanded. Severely infected plants reached 37.4% of whole plant covered with scab. Numerous conidia were produced on the diseased flower disk, pod, floral axis, stem and leaves. Most of the conidia were appeared to be readily dispersed in the air, but the mycelia were not suggested causing of sooty mold by ectoparasitism. A fungus was isolated from the diseased stem, and inoculated to healthy plants to satisfy the Koch's postulates and proved the fungus was the causal agent of the disease. The isolated fungus grew on potato dextrose agar, forming greenish black to pale brown colonies. Conidia were ellipsoidal, fusiform or subspherical, mostly one-celled but occasionally septated. The conidia were $3.9{\sim}34.1{\times}2.7{\sim}5.1\;{\mu}m$ in size and formed in long branched chains on the erected conidiophores which were pale olivaceous brown and variable in length between $7.2{\sim}210.7\;{\mu}m$ in size. Ramoconidia were $7.6{\sim}29.2{\times}3.2{\sim}14.4\;{\mu}m$ in size. The fungus was identified as Cladosporium cucumerinum based on the above morphological characteristics. The optimum temperature for mycelial growth and conidial formation was about 15 to $25^{\circ}C$. Cladosporium scab of sword bean caused by the fungi has not been reported in Korea previously.

• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.331-338
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• 2016

The present study aimed to compare and investigate the morphological characteristics and yield components according to ploidy level of diploid and tetraploid Platycodon grandiflorum under vinyl-greenhouse and open field conditions. Plant height of diploid and tetraploid P. grandiflorum was 51.3 ㎝, 54.0 ㎝, respectively. The results revealed that the plants grown in the vinyl-greenhouse showed significantly higher growth compared to those grown in the open field. Regardless of the growing place, diploid and tetraploid of P. grandiflorum showed the rapid elongation of internodes after 4 and 3 internodes respectively and elongation tends to be decreased as entering the flower-bud differentiation period. The starting day of flowering in vinyl-greenhouse cultivation was found to be faster than that of the open field cultivation by 2∼3 days and tended to be delayed by about 5∼6 days in tetraploid P. grandiflorum compared to diploid. Fresh weight of roots from the vinyl-greenhouse cultivation showed a high quantity as 34.2g and 49.4g in diploid and tetraploid P. grandiflorum, respectively and especially tetraploid P. grandiflorum was found to be increased by approximately 44.4% compared to other plots.

• Korean Journal of Plant Resources
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• v.15 no.2
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• pp.89-95
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• 2002

In woody plant germplasms, using prefrozen dormant buds for materials is one way to achieve successful cryopreservation. The protocol of cryopreservation for White birch (Betula platyphylla var. japonica) winter vegetative buds is the following. First, the branches of White birch were collected in January 20, when the vegetative buds were still in a state of quiescence. The winter buds with about 5㎜ of xylem tissue were removed from the branches. They were dehydrated to moisture contents about 44% by air dry treatment. The buds were prefrozen, with the temperature being decreased by 5∼-20$\^{C}$ and then transfered to the LN(liquid nitrogen) maintained below -l96$\^{C}$. After cryopreservation, the vegetative buds were rapidly thawed in a water bath at 40$\pm$5$\^{C}$. In this case, the cell survival rate of samples was about 86%. After sterilization, buds were then cultured on MS medium. These results demonstrate the feasibility for cryopreservation of winter vegetation buds of Betula platyphylla var. japonica.

• Korean Journal of Medicinal Crop Science
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• v.18 no.2
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• pp.93-97
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• 2010

Cultural practices of Asparagus cochinchinensis in highland area were performed for a potential medicinal crop. These studies were examined to propagation methods, planting densities, nitrogen treatments, and cropping years. The results are summarized as follows. The adequate number of buds per tuberous root was 4 for vegetative propagation because the number of tuberous root harvested was 16.8 and the yield was also the highest, exhibiting 1,060 kg/10a. The suitable planting time for vegetative propagation was later than early April. If the earlier tuberous roots were planted, the less they emerged. The highest emergence rate was obtained from the planting density of $30{\times}20cm$ as 97.2% while the yield was highest in the $30{\times}15cm$ density, exhibiting 1,883 kg/10a with emergence rate at 94.9. It seemed that the higher planting density promoted plant height growth and yield in Asparagus cochinchinensis. The highest fresh weight was recorded at 6 kg/10a of nitrogen fertilizer into the sandy loam soil compared to the level of 0, 3, 9 kg/10a. The yield was increased with cropping years. However, the proper harvesting time was the second year of cultivation because the rate of weight increase was maximized in the 2-year-old tuberous root. The yield in the third year was decreased as compared to that of the second year.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.645-648
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• 2010

A new spray carnation cultivar 'Lyra' was released by the National Institute of Horticultural and Herbal Science in 2003. A cross was made between spray cultivar 'Ballantyne' and red spray cultivar 'Chateau' in 1999. After investigation of the characteristics during four years (from 2000 to 2003), the cultivar 'Lyra' was finally selected in 2003. 'Lyra' is a spray cultivar with bright pink color for a cut flower. The main characteristics are early flowering, multi branching, fragrance and long flower stalk. Also, it has medium resistance to Fusarium wilt and 7 days vase life. To avoid calyx splitting caused by high temperature, it is recommended that this cultivar be grown over $8^{\circ}C$ at night and under $25^{\circ}C$ at day time during a flower bud differentiation period and applied with additional nitrate fertilization ($30.0kg{\cdot}10^{-1}$).

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.815-826
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• 2018

The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.2
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• pp.195-200
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• 1985

This experiment was carried out to know the processes of protoplast isolation, culture and plant regeneration in aims of introducing foreign genes into plant cells through plant gene vector, and cellular selection for plant improvement. The main results indicated that 2% cellulase plus 0.5% macerozyme is proper for isolation of protoplasts from leaf mesophyll cells of N. plumbaginifolia, plating efficiency was higher in 1.4-2.0 x 10$^4$ cells/ml, complete cell wall was regenerated after 2 days culture, cell division and cell mass were observed after 4 days and 2 weeks, respectively, colony was developed after 3 weeks culture, addition of 1-2mg/l BA promoted shoot differentiation while root differentiation did not required hormone and seeds were harvested from more than 100 cell lines for further investigation and study.

• Korean Journal of Environmental Agriculture
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• v.32 no.1
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• pp.55-60
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• 2013

BACKGROUND: The buds of persimmon trees are susceptible to cold damage, often with the late frost, at the time of budburst. This study was conducted to determine effect of the cold damage on shoot and fruit growth the current season. METHODS AND RESULTS: 'Fuyu' trees, grown in 50-L pots, were placed for 1 h at $-2.2{\pm}0.5$, $-2.6{\pm}0.5$, or $-3.0{\pm}0.5^{\circ}C$ within a cold storage, at their budburst on April 5. Some trees under ambient temperature at $10-17^{\circ}C$ served as the control. Cold damage of the buds containing flower buds was 54% at $-2.2^{\circ}C$, and significantly increased to 95% at $-3.0^{\circ}C$. The bud damage included the complete death of all, complete death of main buds only, or the late and deformed shoot growth in the spring. Number of flower buds in early May dramatically decreased as the damage ratio increased. Since the thinning of flower buds in mid-May and fruitlets in early July was done in no or slightly damaged trees, the final number of fruits and yield did not decrease compared with the control when the damage increased by 60% and 70%, respectively. Average fruit weight and skin coloration tended to be better with increasing bud damage. Shoot growth was more vigorous in those trees whose buds were severely damaged by low temperature. CONCLUSION(S): Shoot growth and the yield may depend on the number of flower buds and percent fruit set after the cold damage.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.415-421
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• 1997

The objectives of this study were to obtain standardized basic data on cambial electrical resistance (CER) of ornamental trees to be used for estimation of tree vigor and to compare CER between shrubs, deciduous, and coniferous trees in relation to bud opening, flowering, and growth cessation. Eighty healthy trees with four trees each for twenty ornamental tree species growing at an university campus in Suwon were selected and their CER was measured using a Shigometer every week from March to May, once a month from June to October and every two weeks in November, 1996. The CER of all tree species was high in March, started to decrease in April, maintained minimum in May through August, began to increase in September, and returned in November to the similar high level to March. Among the 20 species, Metasequoia glyptostroboides showed the lowest($5.5k{\Omega}$) annual average CER, while Cercis chinensis and Ligustrum obtusifolium had the highest($22.7k{\Omega}$, $22.9k{\Omega}$) annual average CER. The lowest CER($2.4k{\Omega}$) in summer(June through August) was obtained from Wisteria floribunda, second lowest($2.5k{\Omega}$) from Metasequoia glyptostroboides, the highest CER($46.8k{\Omega}$) during dormant season from Euonymus japonica, and second highest($45.0k{\Omega}$) obtained from Ligustrum obtusifolium. The CER of most species, particularly shrub species, started to decrease with bud opening, and many species, particularly shrubs and deciduous trees with large amount of flowers, showed sharp decrease with flowering. When CER was compared between shrubs and trees, shrubs showed higher average CER than trees, and seasonal difference in CER of evergreen species was smaller than that of deciduous species. It is interesting to note that the deciduous Metasequoia glyptostroboides had the lowest annual average CER.