• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.243-251
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• 2007

It has been recently demonstrated the presence of not only $C_{28}-BRs$ biosysnthesis, but also $C_{27}-$ and $C_{29}-BRs$ biosynthesis in plants, suggesting that BRs biosynthesis are complicatedly connected to produce biologically active BR (s). This prompted us to investigation of metabolism of a $C_{29}-BR$, 28-homoCS in seedlings of rice from which $C_{29}-BRs$ such as 28-homoTE and 28-homoTY have been identified. In vitro enzyme conversion study using a crude enzyme solution prepared from rice seedlings revealed that 28-homoCS is converted into both CS and 26-nor-28-homoCS, but their reversed reaction did not occur. This indicated that 28-homoCS is biosynthetically converted into more biologically active $C_{28}-BR$, CS by C-28 demethylation and biodegraded into 26-nor-28-homoCS by C-26 demethylation. Next, bio-conversion of 28-homoCS to 28-homoBL was examined by the same enzyme solution. No 28-homoBL as a metabolite of 28-homoCS was detected, meaning that biosynthetic reaction for 28-homoCS to 28-homoBL is not contained, and main connection of $C_{28}-BRs$ and $C_{29}-BRs$ biosynthesis is between CS and 28-homoCS in the rice seedling. This study is the first demonstrated that $C_{29}-BRs$ and $C_{28}-BRs$ bionsynthetic pathways are connected, and that $C_{29}-BRs$ biosynthetic pathway is an alternative biosynthetic pathway to produce more biologically active $C_{28}-BR$, CS in plant.

• Annals of Clinical Neurophysiology
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• v.12 no.2
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• pp.61-65
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• 2010

Background: Orthostatic hypotension (OH) refers to a fall in systolic blood pressure (BP) of 20 mmHg or more, or in diastolic BP of 10 mm Hg or more within 3 minutes of standing up. The head-up tilt test (HUT) is the most useful, but potentially invasive test for the diagnosis of OH. The purpose of this study was to identify the usefulness of spontaneous baroreflex sensitivity (sBRS). Methods: Ninety one patients with orthostatic intolerance, in whom the HUT data were available, were included in the study. Patients were classified into HUT-positive (group I) and HUT-negative (group II) group. Twenty five healthy volunteers served as normal controls, and were designated as group III. In all subjects, beat-to-beat BP and heart rate were recorded using BeatScope 1.1a. We collected the 50 sBRS data in each patient in a supine position. The average value of one to ten of 50 sBRS data was defined as sBRS10, one to twenty as sBRS20, one to thirty as sBRS 30, one to forty as sBRS 40, and one to fifty as sBRS 50. Differences in sBRS10 and sBRS50 levels were statistically analyzed and compared between groups I, II, and III. Results: No significant difference in the sBRS50 level was found between Groups II and III. sBRS50 was significantly lower in Group I than in Groups II and III (p<0.05), and the same pattern of differences was observed for sBRS40, sBRS30, sBRS20, and sBRS10. Conclusions: Patients with OH showed significantly lower sBRS levels than HUT-negative patients or normal controls. Our study implies that a supine-position sBRS would provide additional diagnostic information for OH.

• Journal of Internet Computing and Services
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• v.2 no.2
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• pp.51-63
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• 2001

In this paper, we design and implement a XML document retrieval system to support structure-based retrieval using the BRS/Search system that is a commercial search engine, The implemented system in this paper represents the logical structure of XML documents as the directory structure of the Unix file system. In addition, we define the database schema of BRS/Search system to store documents, We also implement a ETID extractor, a structure information extractor, a storage manager and a query processor additionally to support content retrieval, structure retrieval, mixed retrieval and attribute retrieval in the BRS/Search system.

• Journal of Biomedical Engineering Research
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• v.31 no.1
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• pp.14-19
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• 2010

Baroreflex sensitivity (BRS) is a parameter of the cardiovascular system that is reflected in changes in pulse interval (PD and systolic blood pressure (SBP). BRS contains information about how the autonomic nervous system regulates hemodynamic homeostasis. Normally the beat-to-beat SBP measurement and the pulse interval measured from the electrocardiogram (ECG) are required to estimate the BRS. We investigated the possibility of measuring BRS in the absence of a beat-to-beat SBP measurement device. Pulse arrival time (PAT), defined as the time between the R-peak of the ECG and a single characteristic point on the pulse wave recorded from any arterial location was measured by photoplethysmography. By comparing the BRS obtained from conventional measurements with our method during controlled breathing, we confirmed again that PAT and SBP are closely correlated, with a correlation coefficient of -0.82 to -0.95. The coherence between SBP and PI at a respiration frequency of 0.07-0.12 Hz was similar to the coherence between PAT and PI. Although the ranges and units of measurement are different (ms/mmHg vs. ms/ms) for BRS measured conventionally and by our method, the correlation is very strong. Following further investigation under various conditions, BRS can be reliably estimated without the inconvenient and expensive beat-to-beat SBP measurement.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.26-32
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• 2013

Butanol-resistant bacteria were isolated from butanol solvent. The cell growth of isolated strains declined with increasing concentrations of butanol, and isolated strain BRS02 displayed more resistance to 12.5 g/L of butanol than other isolated strains. In addition, strain BRS251, which was resistant to even higher concentrations of butanol, was developed by the mutation of BRS02 using UV. BRS251 could grow in LB medium containing up to 17.5 g/L of butanol, 32.5 g/L of propanol, or 6 g/L of pentanol, whereas the control strain Escherichia coli was found to be tolerant to 7.5 g/L of butanol, 20 g/L of propanol, or 2 g/L of pentanol. The isolated BRS02, a Gram(+) bacterium seen to have a cocci form under the microscope, grew in 6.5% NaCl. According to biochemical tests, BRS02 can metabolize and produce acid with D-galactose, D-maltose, D-mannitol, D-mannose, methyl-${\beta}$-Dglucopyranoside, D-ribose, sucrose, or D-trehalose, as carbon sources. Also, this strain showed resistance to bacitracin, vibriostatic agent O/129, and optochin, alongside positive activities for arginine dihydrolase, ${\alpha}$-glucosidase, and urease. The BRS02 strain was identified as Staphylococcus sp. by analyses of the 16S rRNA gene, phylogenetic tree, and biochemical tests.

• Journal of Life Science
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• v.21 no.7
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• pp.969-975
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• 2011

We focused on relationship between phytohormones and AtEXPA3 gene in gravitropic response of A. thaliana. RT-PCR analysis shows that AtEXPA3 was highly expressed in actively developing tissues such as leaf, rosette, root and flower tissues. AtEXPA3 gene expression was enhanced by gravistimulation, BR and IAA. Furthermore, decreased gravitropism was observed when treatment of AVG, an ethylene biosynthetic inhibitor, suggesting that ethylene has a gravistimulating effect itself as well as BRs and IAA. Inhibition of gravitropism in AtEXPA3 RNAi mutant suggests that BR, auxin and ethylene are playing roles as regulators of AtEXPA3. In addition, altered gravitropism in BRs signaling mutant (decreased in bri1-301, bak1, and increased BRI-GFP) indicated that BRs signaling mediated the gravitropism. In conclusion, gravitropic responses of Arabidopsis root resulting from root growth were mediated by increased expression of AtEXPA3 gene, which is stimulated by phytohormones.

• Journal of Integrative Natural Science
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• v.1 no.1
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• pp.24-31
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• 2008

Brassinosteroids (BRs) are a special class of plant steroid hormones that are essential for normal growth and development. Part of confusion is whether BRs are unique to plants, because they have overlapping physiological roles with other better-studied hormones and with physiological responses caused by light. In systems designed to assay for cytokinins, the effects of BRs vary. We measured hypocotyl length for testing the ability of brassinolide (BL) to rescue double mutant between det2 and the photoreceptor null mutant phytochrome B (phyB). PHYB involved in controlling hypocotyl elongation in increased concentration of BL whereas phyBdet2 double mutant just partially rescue to phyB in white and red light indicated the involvement of BRs in PHYB regulated cell elongation. BRs regulated hypocotyl growth was delayed by BAP, a cytokinin treatment but inhibitory effects of BAPs on hypocotyl growth was slightly recovered by BL. The result indicated that the mode of action of BR and cytokinin is independent or sequential in the downstream light-regulated response control on hypocotyl elongation and also light modulated the action of BR and cytokinin in some extent.

• Journal of the Semiconductor & Display Technology
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• v.21 no.4
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• pp.50-52
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• 2022

Brownish rings (BRs) with white interiors are formed during the manufacture of silicon ingots in quartz glass crucibles. These BRs inhibit the yield of silicon ingots. However, the composition and mechanism of the formation of these BRs remain unclear thus far. Therefore, in this study, we analyzed the color and shape of these BRs. Raman analysis revealed that the brown and white colors appear owing to oxygen deficiency rather than crystallization from excess oxygen supply as previously assumed. Moreover, the dark shade of the brown areas depends on the degree of oxygen deficiency and the asymmetrical width of the brown areas is attributed to the direction of the molten silicon flow, which is influenced by the rotation and heat of the ingot crucible.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.23 no.2
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• pp.50-68
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• 2024

This study developed a bus route sketch (BRS) methodology for utilizing bus route design and operation steps in practice and evaluated the feasibility of the method. The BRS methodology consists of three steps: transportation zoning suitable for the provider and users of bus transit service; determining the bus operation route based on established transportation zones and path combination; optimizing the operation route based on the estimation of route alternatives in terms of the multi-performance measures from the standpoints of bus-transit service provider and user. The results of a case study showed that the estimation scores from the perspectives of provider and user were improved significantly from 8.83 and 7.13 to 9.50 and 9.89, respectively. Because the BRS method was designated and developed to be suitable for field application for route planning and operation, the method can be used instantly and directly to estimate and adjust the on-operation bus transit line and route design.

• Korean Journal of Food Science and Technology
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• v.53 no.6
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• pp.731-738
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• 2021

This study investigated the physicochemical properties of rice starch isolated from broken rice using alkaline steeping (AKL) and enzymatic digestion (ENZ) methods. Broken rice starch (BRS) by AKL and ENZ possessed crude protein contents (0.6-1.4%) acceptable to commercial products of native starch and belonged to an intermediate amylose rice starch. AKL-BRS and ENZ-BRS showed a typical A-type crystal packing arrangement with small variations in their relative crystallinity. ENZ-BRS exhibited higher gelatinization onset and peak temperatures, and a narrower gelatinization temperature range than AKL-BRS, indicating that annealing occurred in ENZ-BRS. Lower swelling power and solubility were generally observed in the ENZ-BRS. ENZ-BRS also showed slower viscosity development, higher peak and trough viscosities, and lower breakdown, final, and setback viscosities, compared to those in AKL-BRS. These results are ascribed to the annealing phenomenon in ENZ-BRS. Overall, BRS from cheap broken rice using AKL and ENZ could contribute to the expansion of rice starch utilization in food and non-food industries.