• The Korean Journal of Mycology
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• v.47 no.2
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• pp.143-152
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• 2019

A new cultivar 'Sanjanghyang' was bred from monokaryotic strains of 'Sanbaekhyang' and 'Jangan 1ho'. Pileus was flat, round, and reddish brown. The diameters of the pilei and stipe length of the fruiting bodies were 67.1 mm and 16.9 mm, respectively. The scales were white or slightly brown and distributed evenly. The gill density was sparse and showed a rippled texture. The stipe was cream in color and the fluff was medium. 'Sanjanghyang' had a short cultivation period and fruiting bodies occurred sporadically. Temperature for fruiting body formation was a medium, between 15 to $19^{\circ}C$. 'Sanjanghyang' was different from 'Sanbaekhyang' with regard to its pileus diameter (67.1 mm) and autumn and spring fruiting body production period. 'Sanbaekhyang' had pileus diameter of 74.7 mm, and fruiting body formation occurred in spring and autumn. The rate of fruiting body formation was 89% (first flush), 4% (second flush), and 7% (third flush).

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.18-18
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• 2021

Gomchwi using stuffed leaves is many cultivated for 'Gondalbi(Ligularia stenocephala)' among Gomchwi species. 'Gondalbi' species like to be cultivation on farm because of low the incense and the bitter taste, and high yield. But 'Gondalbi' caused to curtail yield that susceptibility of powdery mildew disease and shriveling and death of plant on summer season. To solve this problems, we crossed a Gomchwi and 'Handeari-gomchwi' to have resistance of powdery mildew disease and high yield. A new Gomchwi cultivar 'Jinmani' was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2007 to 2020 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. trichome of petiole and leaf back non-existed, and luster of leaf back existed. Density of leaf vein was 4 degree among 1-5 degree in a newly developed cultivar 'Jinmani'. Plant height, leaf length, leaf width and petiole length were 55.7, 21.8, 22.2, and 33.9 cm, respectively in the 2nd year of growth characteristics. Plant size was similar with that of 'Gommany'. Bolting and flowering time were Aug. 5 and Sept. 5, respectively, and Bolting and flowering time of 'Gommany' showed similar to Aug. 8 and Sept. 1, respectively. 'Jinmani' showed higher number of leaves (202 ea.) per plant compared to 'Gommany' (159 ea.). Furthermore, yield was 67.9% higher in 'Jinmani' (2,569 g/plant) than in 'Gommany' (1,530 g/plant). 'Jinmani' showed lower leaf thickness (0.66mm) than 'Gommany' (0.69 mm), and consequently showed more hardness in leaf characteristics (25.1 kg/²) compared to 'Gommany' (24.3kg/cm²). 'Jinmani' showed similar strong resistance compared to 'Gommany' in the susceptibility of powdery mildew disease.

• Korean Journal of Environment and Ecology
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• v.34 no.1
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• pp.9-17
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• 2020

Domestic cats (Felis catus) introduced to insular environments can be invasive predators that often threaten endemic species and cause biodiversity loss or local extinction on the island. This study was conducted from March to July 2018 to understand the population size, home range, and spatial use of cats introduced to Mara Islet (N 33° 07', E 126° 16') in Jeju Special Governing Province, the Republic of Korea. Observation records based on their natural marks revealed that there were 20 adult cats on Mara Islet. A capture-recapture method also estimated 20 adult individuals (95% confidence interval: 20-24 individuals). According to our telemetry study on ten adults deployed with GPS-based telemetry units, the home range size was 12.05±6.99 ha (95% KDE: kernel density estimation), and the core habitat size was 1.60±0.77 ha (50% KDE). There were no significant differences in the home range and core habitat sizes by sex. The home range of domestic cats overlapped with the human residential area, where they might secure easy foods. Five of ten tracked cats were active at potential breeding colonies for the Crested Murrlet (Synthliboramphus wumizusume), and six approached potential breeding areas of the Styan's Grasshopper Warbler (Locustella pleskei), suggesting the predation risk of the two endangered species by cats. This study provides novel information on the population size and home range of introduced cats on Mara Islet which is an important stopover site of migratory birds as well as a breeding habitat of the two endangered avian species. Reducing the potential negative impacts of the introduced cats on migratory birds and the endangered species on Mara Islet requires monitoring of the predation rate of birds by cats, the population trends of cats and endangered breeding birds as well as the effective cat population control and management.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.33-48
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• 2012

As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.329-335
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• 2014

This study was performed to study characteristics of new zoysiagarass cultivar 'Green Ever' (Plant Variety Protection Application no. 2014 - 02). 'Green Ever' was developed by selection breeding among 100 native zoysiagrasses collected in South Korea. 'Green Ever' showed specific bands (1.48 kb with OPC14 and 1.05 kb with OPD5) which were distinct from 'Anyang Joonggi' or 'Dongrae Koryogi' in RAPD analysis. 'Green Ever' was classified as Z. matrella in morphological evaluations including plant height ($6.8{\pm}0.5cm$), leaf width ($2.0{\pm}0.1mm$), height of lowest leaf ($1.9{\pm}0.1cm$) and seed length ($3.0{\pm}0.1mm$), which was similar with morphological characteristics of 'Dongrae Koryogi'. Turf quality, shoot density and chlorophyll content of 'Green Ever' were higher than them of 'Anyang Joonggi' and 'Dongrae Koryogi' under intensive management such as fairway on golf course, especially shoot densities of 'Green Ever' were excellent higher than 'Anyang Joonggi' and 'Dongrae Koryogi' during experimental periods. It could be extensively used in fairway, teeing ground of golf courses and landscape garden after further study about various environmental adaptabilities such as winter kill, wear tolerance etc.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.462-469
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• 1982

Since 1962, varietal development and dissemination for summer upland crops have been actively initiated by the Crop Experiment Station, Office of Rural Development, Suweon, Korea. The major breeding objectives of soybeans have been to develop varieties which are early maturing, disease resistant, and adaptable to late planting for after-barley cropping. Development of eleven new soybean varieties including Hwangkeumkong, Jangyeobkong, Danyeobkong, and Kwangkyo has greatly increased the soybean yield throughout country. For com, after development of Hwangok #2, a synthetic, in early 1960's, nine corn hybrids-single crosses, double-crosses, and three-way crosses-such as Jecheon-ok, Hoengseong-ok, Kwangok, Suweon #19, etc., have been disseminated mainly to Kangweon province, a major corn producing area in Korea, and drew up the yield over 4 tons per hectare. The major breeding objectives of sweet potato have been to develop varieties which have high starch content and root yield. Hwangmi, Hongmi, and Shinmi are three sweet potato varieties developed and disseminated by the Crop Experiment Station, Office of Rural Development and are grown most widely in Korea. Most of researches on cultural practices of upland crops have begun on a full scale from early 1960's. In soybeans, for example, no fertilizer but for barley was applied although the effects of phosphate and potassium fertilizers were great on soybeans in after-barley soybean croppings. The effects of heavy application of phosphate and calcium fertilizers on soybeans in newly reclaimed soils were recognized. Recently a mixed fertilizer for soybean (N; 40, P:70, K:60 kg/㏊) was developed and sold for soybean growers. The optimum planting densities of 220, 000 plants/ha in full-season cropping and 330, 000 pts/ha in after-barley cropping of soybeans were known from repeated experiments. For higher yield, a means of cultural practices such as transplanting-pinching, direct planting-pinching, and hilling-up, etc., were developed along with barley-stubble planting with no tillage and integrated herbicide application for labour savings. For sweet potato, cultural practices for planting date, harvesting date, fertilizer, and planting density were fully established. For early marketing, a technique of vinyl-mulching on sweet potato has also fully developed. For com, planting density of 37, 000 pts/ha in early 1960's has been changed to 55, 000 pts/ha for grain production and 67, 000 pts/ha for silage. The amounts of fertilizers have also been changed from 120-120-120kg/ha (N-P-K) in early 1960s to 180-150-150 kg/ha. These increases in number of plants per unit area and fertilizer levels have resulted in greater production for both grain and silage. At the same time, the production techniques of F1 seeds have also improved.

• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.133-144
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• 2007

This study was conducted to determine the effective environmental control measures in preventing calf diarrheal disease in farms. It was found that the high numbers of calves in a cow house was directly related with the high incidence rate of diarrheal diseases. When the number of calves was increased in a cow house, it was difficult to reduce the diarrheal diseases of calves. It was revealed that the incidence rates of calf diarrheal disease were dramatically reduced when the delivery house which faced south. The periodical changes of bedding materials seemed beneficial good to control the possible resident infectious agents, including pathogenic viruses and bacteria. The control of the delivery time was important for reduction of calf diarrhea. It was revealed that September and October were the best time as the delivery period. In order to use old cow house as a delivery house, it should be sterilized by the disinfectant and the flame. When the diarrheal feces were removed as soon as possible, the transmission of diarrheal diseases among calves In the same house was reduced. When the diarrheal feces were not disposed, the incidence rate of diarrhea reached up to 50%. When the morbid calves were not quarantined, the incidence rate of diarrhea was 100%. In contrast, when the diseased calves were quarantined from other calves, the incidence rate of diarrhea was reduced, remarkably. The mean recovery time from the diarrhea of the treated calves in the southern cow house was much faster than that in the north cow house. The treatment of calves in a clean and well-ventilated cow house with dry bedding was more effective than that in a cold, wet, and dark house. It could be postulated that the most important environmental factor in the reduction of diarrheal diseases of calves is the blocking of the entry of the infectious agents. The complete sanitation and control of the delivery house in the using farm is more important than building a new cow house, for prevention of calf diarrheal diseases.

• Korean Journal of Breeding Science
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• v.42 no.2
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• pp.157-162
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• 2010

This study was carried out to develop of macro-protocol and the biosafety guide for 'Nakdong', and 'Golden Rice' (genetically modified vitamin A rice) by large scale field trial of GM crops. Typically, when a new GM crop is created, breeders should conduct field test to make sure the GM crop is safe, and provide some information on GM crops for approval. A total of isolated 4,700 $m^2$ field for trial of GM crops were prepared, and 'Nakdong' and 'Golden rice' were cultivated by standard method of RDA (Rural Development Administration, Korea). Field studies indicated that the population densities of insect pests and natural enemies have no difference between two varieties. While insect pest density on 'Nakdong' was slightly higher than on Golden Rice, but natural enemy density on Golden Rice was a little higher. These results provided the insect diversity for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.135-146
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• 2011

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.326-335
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• 2015

Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.