• Korean Journal of Environment and Ecology
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• v.37 no.1
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• pp.35-58
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• 2023

In this study, we conducted a flora survey at Mt.Bakdal (363m) and Mt. Myeongbong (245.3m) in Paju-si, Gyeonggi-do. A total of 405 taxa, including 2 forms, 32 varieties, 8 subspecies, 363 species, 261 genera, and 87 families, were surveyed in the two mountain areas. The ferns were identified as 12 taxa from 6 families, the gymnosperms as 6 taxa from 2 families, the dicotyledonous plants as 290 taxa from 70 families, and the monocotyledonous plants as 97 taxa from 9 families. The remarkable plants included 8 taxa of the Korean endemic plants, which were Populus tomentiglandulosa, Salix koriyanagi, Clematis brachyura, Viola seoulensis, Forsythia koreana, Paulownia coreana, Weigela subsessilis, and Hemerocallis hakuunensis, and 3 taxa of the rare and endangered plants as designated by the Korea Forest Service, including Tylophora floribunda as Vulnerable (VU) species, and Viola albida and Chionanthus retusus as Least Concern (LC) species. The invasive alien plants were 35 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Chenopodium album, Chenopodium ficifolium, Lepidium virginicum, Amorpha fruticosa, Robinia pseudoacacia, and Trifolium repens, accounting for 8.5 percent of the 405 taxa of the surveyed plants. We intend to further identify plants through the studies on unsurveyed areas in the western part of the DMZ, identify the native habitats of rare and specialty plants, and use them as basic data for managing ecosystem-disturbing plants.

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.381-389
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• 2023

The germination characteristics of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz., a rare and endemic plant designated by the Korea Forest Service, were investigated according to light conditions, temperature, and pretreatment. As a result of the germination experiment according to light conditions, it was determined that P. kiusianum is a photoblastic seed that does not germinate at all in dark conditions. The optimum germination temperature of the seeds was found to be 20-25℃, considering the final germination rate and germination time. Three growth regulators (IAA, GA₃, kinetin) and two inorganic salts (KNO₃, KCl) were pretreated to improve the germination rate of P. kiusianum seeds. The growth regulators IAA and kinetin had no significant effect on improving the germination rate of P. kiusianum seeds. On the other hand, GA₃ significantly increased the final germination percentage and germination rate regardless of the concentration, especially the treatment of more than 500 mg·L^-1 at 20℃ was more than 4 times more effective than the untreated. The inorganic salts KNO₃ and KCl had no significant effect on the seeds of P. kiusianum at low concentrations, but at higher concentrations (40 mM and 300 mM, respectively), they improved the germination rate and germination age by 2 times compared to the untreated. The results of this study will be useful for the mass propagation of P. kiusianum, which has the potential to be utilized as a native plant for restoration.

• Korean Journal of Plant Resources
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• v.37 no.2
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• pp.171-202
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• 2024

If the plant genetic resources and information-sharing systems held by arboretums worldwide are effectively utilized, it is believed that a conservation system for plant diversity in the currently inaccessible North Korean region could be established. This study was conducted to review the scientific names of plants native to North Korea but not to South Korea and to assess the status of genetic resources held in domestic and international arboretums. To compile a list and status of North Korean plant's genetic resources, updated checklists of vascular plants in Korean Peninsula and online plant information databases were consulted to compile synonym, distribution range, and other related information. A total of 486 taxa (449 species, 13 subspecies, 21 varieties, 1 forma and 2 hybrids) from 236 genera and 64 families, representing 12.34% of the total native flora of the Korean Peninsular were presented in the North Korea plant list, and the presence of rare, endemic and northern lineage species was confirmed. It was found that 384 taxa from 190 genera, 53 families of North Korean plants are held as genetic resources in 333 arboretums and plant research institutions across 46 countries and 5 continents worldwide. This study is expected to contribute to the construction and application of a species list for plants native to the Korean Peninsula.

• Journal of Korean Society of Rural Planning
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• v.15 no.4
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• pp.173-182
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• 2009

This study is a plan for the Jeju Sanghyo Botanical Garden construction, and it is prepared to maintain the identity of the local landscape by composing the garden using the local landscape resources. The goals of becoming one of world's best, garden centered botanical garden and at the same time one that represents culture of Jeju. Unusual species are included in the collection and with the general collection, special collection and exhibition by different kinds are planned to be offered to the public. The garden will demonstrate the world's various types of gardens in a compressed way. It is our endeavor to become center of culture beyond plant education and exhibition, drawing a large number of people to our garden. Moreover, conservation of Jeju's unique botanic resources will be carried out and the garden will show the garden culture. Due to the size of the site, different themes will be exhibited in one zone in a parallel. Unique combination of each local landscape resource will create own theme. The display will be divided into three different stages: the 1st stage is an educational exhibition, assisting more understanding of distinguishing different locations, the second is exhibition by various species and the final stage will show an ideal way to develop a garden with harmony of various plant species.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.92-92
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• 2018

Silene seulensis Nakai was used as traditional medicines in Korea, we collected plant from demilitarized zone (DMZ). S. seulensis was extracted with 30, 50, and 70% ethanol and partitioned successively with n-hexane, EtOAc, dichloromethane and BuOH. These extracts (30, 50 and 70% ethanol) were evaluated the cytotoxicity on B16F10 and Hacat cell lines. The LC-MS/MS data of each fractions (n-hexane, EtOAc, dichloromethane, and BuOH) were compared with MS library, combined with ultraviolet/visual (UV/Vis) and MS data for faster determine structure by database search results. This led to the identification of four compounds (1-4) from S. seulensis. These compounds was isolated first time from S. seulensis. Their chemical structures are elucidated by combinations of NMR and mass spectrometry techniques.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.102-102
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• 2019

This study developed a HPLC analysis method for the determination of avicularin (AL) and quercitrin (QT) in Astilbe chinensis by HPLC. HPLC was performed on a Capcell Pak C18 MGII column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$ and a UV detection wavelength of 254 nm. The analytical method was validated for its specificity, precision, accuracy, and linearity. The limits of detection and quantitation were AL 0.09 and 0.28 mg/mL, QT 0.03 and 0.09 mg/mL respectively. Calibration curves showed good linearity (AL r2 > 0.99990 and QT r2 > 0.99994), and the precision of analysis was satisfied (less than AL 0.59%, QT 0.63%). Recoveries of quantified compounds ranged from AL 100.57 to 102.08%, QT 99.94 to 100.64%. These results indicate that the developed HPLC method was applied successfully to the detection of AL and QT in A. chinensis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.100-100
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• 2019

This study attempted to establish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh extracts. HPLC was performed on a Unison US-C18 column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r2 > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.580-587
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• 2023

Androsace septentrionalis is a grass species restricted to North Korea; however, it is at the brink of extinction due to habitat loss and environmental changes caused by natural disasters and anthropogenic activities. This study was conducted to characterize the dormancy conditions of A. septentrionalis in an effort to conserve this North Korean plant resource. For this purpose, the morphological characteristics and vigor of A. septentrionalis seeds were examined, and its germination characteristics under different temperature conditions (15/6℃ and 25/15℃ ), low-temperature stratification, and gibberellin (GA₃) treatment were determined. The results revealed that A. septentrionalis exhibits non-deep type morpho-physiological dormancy, and low-temperature stratification treatment was not effective in breaking the dormancy of A. septentrionalis seeds. Meanwhile, GA₃ treatment significantly increased the mean germination time, rate, and speed of the seeds. The optimal conditions for the germination of A. septentrionalis seeds were 25/15℃ fluctuating temperature and 500 mg·L¹ GA₃ treatment. The results of this study are useful for the mass propagation of A. septentrionalis.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.317-345
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• 2022

This study carried out a survey to utilize basic data for the conservation of northern lineage plants that are vulnerable to climate change, targeting algific talus slope of a specific forest biodiversity region in Yeotan-ri, Jeongseon-eup, Jeongseon-gun, Gangwon-do. The survey was conducted by dividing the wind hole into 625 m² inside and 6,925 m² outside based on the wind hole. The vascular flora were identified as 84 families, 203 genera, 296 species, 6 subs., 27 var., 2 for., and a total of 331 taxa. This was found to be about 7% of 4,724 species of vascular plants in Korea. Plants endemic to the Korean peninsula were identified as 19 taxa, including Syringa fauriei, and 11 taxa for rare plants designated by IUCN. Critically endangered grade (CR) was Paeonia obovata 1 taxa, Endangered grade (EN) was Forsythia saxatilis and Prunus choreiana 2 taxa. A total of 78 taxa were florisitc target species, and 19 taxa were classified for indicator plants in the limestone area. As for alien plants, 20 taxa such as Taraxacum officinale were identified, and the naturalization rate was 6.04% and the urbanization rate was 5.33%. Geographical plants of the study area are 2 taxa of Syringa fauriei and Syringa villosa subsp. wolfii.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.30-55
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• 2022

This study conducted a flora survey in the areas of Mt. Sambong (282m) and Mt. Jaung (263m), located in Paju-si, Gyeonggi-do. A total of 434 taxa, including 6 forms, 34 varieties, 11 subspecies, 383 species, 271 genera, and 94 families, were surveyed in the two mountain areas. The ferns were identified as 24 taxa from 10 families, the gymnosperms as 8 taxa from 2 families, the dicotyledonous plants as 322 taxa from 73 families, and the monocotyledonous plants as 80 taxa from 9 families. Five taxa, including Salix koriyanagi, Cimicifuga heracleifolia, Forsythia koreana, Paulownia coreana, and Hemerocallis hakuunensi, were identified as the Korean endemic plants. Eight taxa, including Cimicifuga heracleifolia, Tylophora floribunda, and Iris minutiaurea as Vulnerable species, andAristolochia contorta, Viola diamantiaca, Lithospermum erythrorhizon, Lilium distichum, andArisaema heterophyllumas Least Concern species, were designated as rare and endangered plants designated by the Korea Forest Service. The invasive alien plants were 40 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Silene armeria, Chenopodium album, Chenopodium ficifolium, Magnolia obovata, Potentilla supina, Amorpha fruticosa, Medicago sativa, Robinia pseudoacacia, Trifolium repens, Euphorbia supina, Hibiscus trionum, and Oenothera biennisaccounting for 9.2 percent of the 434 taxa of the surveyed plants. As this area is a mountainous area close to settlement facilities, it is believed that ecological preservation measures and measures of specialized plants should be implemented.