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Effect of magnesium and calcium phosphate coatings on osteoblastic responses to the titanium surface

  • Park, Ki-Deog;Lee, Bo-Ah;Piao, Xing-Hui;Lee, Kyung-Ku;Park, Sang-Won;Oh, Hee-Kyun;Kim, Young-Joon;Park, Hong-Ju
    • The Journal of Advanced Prosthodontics
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    • v.5 no.4
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    • pp.402-408
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    • 2013
  • PURPOSE. The aim of this study was to evaluate the surface properties and in vitro bioactivity to osteoblasts of magnesium and magnesium-hydroxyapatite coated titanium. MATERIALS AND METHODS. Themagnesium (Mg) and magnesium-hydroxyapatite (Mg-HA) coatings on titanium (Ti) substrates were prepared by radio frequency (RF) and direct current (DC) magnetron sputtering.The samples were divided into non-coated smooth Ti (Ti-S group), Mg coatinggroup (Ti-Mg group), and Mg-HA coating group (Ti-MgHA group).The surface properties were evaluated using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy (AFM). Cell adhesion, cell proliferation and alkaline phosphatase (ALP) activity were evaluated using MC3T3-E1 cells. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed. RESULTS. Cross-sectional SEM images showed that Mg and Mg-HA depositionson titanium substrates were performed successfully. The surface roughness appeared to be similaramong the three groups. Ti-MgHA and Ti-Mg group had improved cellular responses with regard to the proliferation, alkaline phosphatase (ALP) activity, and bone-associated markers, such as bone sialoprotein (BSP) and osteocalcin (OCN) mRNA compared to those of Ti-S group. However, the differences between Ti-Mg group and Ti-MgHA group were not significant, in spite of the tendency of higher proliferation, ALP activity and BSP expression in Ti-MgHA group. CONCLUSION. Mg and Mg-HAcoatings could stimulate the differentiation into osteoblastic MC3T3-E1 cells, potentially contributing to rapid osseointegration.

A Study on the Technology Evaluation of Development of New Variety of Citrus Unshiu (감귤 신품종(하양조생)개발의 기술가치의 평가)

  • Ko, Seong-Bo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.127-132
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    • 2016
  • This study evaluated the economic value of the development of a new variety of Citrus unshiu using the income approach, Net Present Value (NPV), Internal Rate of Return (IRR), and Benefit/Cost (B/C) ratio. The new variety of Citrus unshiu was developed by the national institute of horticultural and herbal science in Korea, rural development administration. The technological evaluation of the development of a new variety of Citrus unshiu can be used to improve the efficiency and practicality of the development of a new variety of citrus. From the research results, the technological value of development of a new variety of Citrus unshui was evaluated at 109,455(discount rate=8%, minimum), 195,040(discount rate=4%, maximum), and 145,375(discount rate=6%, average) million won. The IRR was 51.4%, which was greater than the discount rate(4~8%). The NPV was evaluated at 145.3 billion won(discount rate=6%, average), 195.0 billion won(discount rate=4%, maximum) and 109.4 billion won(discount rate=8%, minimum), all of which were greater than 0. The B/C ratio was evaluated at 60.9(discount rate=6%, average), 81.3(discount rate=4%, maximum) and 46.1(discount rate=8%, minimum), all of which were greater than 1. Therefore, the economic validity of the development of a new variety of Citrus unshiu was identified by this technological evaluation.

THE EFFECT OF GOLD ELECTROFORMING PROCEDURE ON GOLD-SILVER-PALLADIUM ALLOY

  • Hwang, Bo-Yeon;Kim, Chang-Whe;Lim, Young-Jun;Kim, Myung-Joo
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.3
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    • pp.303-309
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    • 2007
  • Statement of problem. The effect of gold electroforming on gold alloy was not studied. Purpose. This in vitro study investigate the effect of gold electroforming on gold-silver-palladium alloy. Material and methods. Three pieces of gold strips had undergone the electroforming procedures on one side and then half of the side again electroformed. The set mode for this study was program 1 ($200{\mu}m$). And the processing time was 15min (1/20 time to form $200{\mu}m$ coping). The confocal laser scanning microscope (PASCAL 5, Carl Zeiss, Bernried, Germany) was used to measure the thickness of the pure gold layer electroformed on the gold strips. Half of the gold strip was coated two times with electroformed gold, and the other half one time. The data from the cone focal laser system was processed to get the vertical profile of the strips and the difference of the vertical height between the double coated and single coated layer was regarded as the thickness of the gold coating. The layer thickness value to built 3D image of the cone-focal laser was set $0.5{\mu}m$. Next to the measurement of the thickness of the coating, the Vicker's hardness test was done. It was performed on the double coated surface, single coated surface and non-coated surface (back side) three times each. Results. The mean thickness value gained from gold electroforming technique was measured to be $22{\mu}m$ for sample 1, $23{\mu}m$ for sample 2, $21{\mu}m$ for sample 3. In the same condition of time, power and the amount of electrolyte, the data showed no difference between samples. According to the results of variance analysis, the differences among the variations in number of coating were statistically insignificant (p>0.05), meaning that the two times of gold electroforming coating did not change the hardness of gold-silver-palladium alloy. Conclusion. The test of thickness of gold coating proved the coherency of the gold electroforming procedure, in other words, when the power, the exposed surface area, processing time and the amount of electrolytes were set same, the same thickness of gold would be coated on. The hardness test showed that the electroformed gold coating did not change the hardness of the gold-silver-palladium alloy when it is coated not more than $45{\mu}m$.

Effects of Coronal Thread Pitch in Scalloped Implant with 2 Different Connections on Loading Stress using 3 Dimensional Finite Element Analysis (연결부 형태가 다른 두 가지 scallop 임플란트에서 경부 나사선 피치가 응력 분포에 미치는 영향 : 삼차원적유한요소분석)

  • Choi, Kyung-Soo;Park, Seong-Hun;Lee, Jae-Hoon;Huh, Jung-Bo;Yun, Mi-Jung;Jeon, Young-Chan;Jeong, Chang-Mo
    • Journal of Dental Rehabilitation and Applied Science
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    • v.29 no.2
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    • pp.111-118
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    • 2013
  • Purpose of present study is to investigate the effects of thread pitch in coronal portion in scalloped implant with 2 different connections on loading stress using 3 dimensional finite element analysis. Scalloped implant with 4 different thread pitches (0.4mm, 0.5mm, 0.6, and 0.7mm) in the coronal part was modeled with 2 different implant-abutment connections. Platform matching connection had the same implant and abutment diameter so that they were in flush contact at the periphery while platform mismatching connection had smaller abutment diameter than implant so that their connection was made away from periphery of implant-bone interface. Occlusal loading of 100N force was applied vertically and 30 degree obliquely to all 8 models and the maximum von Mises bone stress was identified. Loading stress as highly concentrated in cortical bone. Platform mismatching scalloped implant with small thread pitch (0.4mm) model had consistently lowest maximum von Mises bone stress in vertical and oblique loads. Platform matching model had lowest maximum von Mises bone stress with 0.6mm thread pitch in vertical load and with 0.4mm thread pitch in oblique load. Platform mismatching connection had important roles in reducing maximum von Mises bone stress. Scalloped implant with smaller coronal thread pitch showed trend of reducing maximum von Mises bone stress under load.

1 Case of Bronchial Leiomyoma with Intralobar Pulmonary Sequestration (폐 격리증을 동반한 기관지내 평활근종 1례)

  • Jung, Bo Yong;Kim, Do Hwan;Park, Eun Seo;Han, Seung Hoe;Kim, Young Tong;Oh, Mi Hye;Lee, Seok Yul;Choi, Jae Sung;Na, Joo Ock;Seo, Ki Hyun;Kim, Yong Hoon
    • Tuberculosis and Respiratory Diseases
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    • v.60 no.6
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    • pp.678-683
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    • 2006
  • Both bronchial leiomyoma and pulmonary sequestration are rare conditions, and to the best of our knowledge there are no reports of the two conditions coexisting. We report a female patient with bronchial leiomyoma with acquired pulmonary sequestration who presented with dyspnea, cough and purulent sputum. The patient had been treated for pneumonia at a local medical clinic. but was transferred to our clinic beacausr there was no clinical improvement. A 3-D computed tomography scan revealed a 1.5 cm sized mass near the distal portion of the left main bronchus and an anomalous artery arising from the aorta. The patient showed clinical improvement after a left lower lobectomy and a ligation of the anomalous artery.

Changes in Microbial and Chemical Composition and Sensory Characteristics of Fermented Soybean Paste, Chungkukjang, by High Dose Gamma Irradiation $(10{\sim}120\;kGy)$ (고선량$(10{\sim}120\;kGy)$ 감마선 조사 청국장의 미생물 변화와 화학적 성분과 관능적 특성 변화)

  • Ahn, Bo-Sun;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.166-172
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    • 2003
  • Changes in the number of microorganisms, chemical composition, and sensory quality of chungkukjang by gamma-irradiation up to 120 kGy were investigated. The total viable cell counts in chungkukjang irradiated with 10 kGy decreased from $10^9\;CFU/g$ of the control to $10^5\;CFU/g$. Proximate chemical composition and pH of chungkukjang were not much affected by irradiation. Acidity of chungkukjang decreased by irradiation with over 20 kGy. Fatty acid contents of chungkukjang irradiated under 20 kGy were the same as those of the nonirradiated ones. Polyunsaturated fatty acids content, decreased at over 40 kGy. The color and odor of chungkukjang were not significantly affected by irradiation up to 20 kGy. Unpalatable odor (p<0.01), off-flavor (p<0.001), and fish-odor (p<0.001) increased, and brown intensity (p<0.05) and acceptability (p<0.001) decreased at over 20 kGy. Results indicate 20 kGy is sufficient to decrease the microbial count of chungkukjang and prevent decrease in the content of polyunsaturated fatty acids.

Beck Depression Inventory Score and Associated Factors in Korean Patients with Lumbar Spinal Stenosis (척주관협착증 환자의 Beck Depression Inventory 점수와 이와 관련된 요인들의 분석)

  • Kim, Ae Ra;Seo, Bo Byoung;Kim, Jin Mo;Bae, Jung In;Jang, Young Ho;Lee, Yong Cheol;Kang, Chul Hyung;Jung, Sung Won;Hong, Ji Hee
    • The Korean Journal of Pain
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    • v.20 no.2
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    • pp.138-142
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    • 2007
  • Background: Depression is a frequent comorbid disease of chronic pain patients. This study was conducted to evaluate the prevalence of depression and to correlate associated factors and depression in patients with lumbar spinal stenosis. Methods: The data of this survey was collected from 97 patients that visited our pain clinic for the management of lumbar spinal stenosis. Depression was examined by a self-reported survey using the Korean version of the Beck Depression Inventory (BDI). The Oswestry Disability Index (ODI) and the life satisfaction scale score were also obtained. Demographic and clinical characteristics (including spouse status, employment status, smoking status, the number of patients with multiple painful areas, the number of patients with combined disease, pain duration, visual analogue scale, Roland 5-point scale and walking distance) were obtained from an interview with the patient. The patients were divided into group N ($BDI{\leq}14$, n = 43) and group 0 (BDI > 14, n = 54) according to the BDI scale. Of the 97 patients, 55,7% had a high BDI score. Results: The patients in group N had a higher rate of employment (48.0%, P < 0.05) and had higher life satisfaction scale scores ($9.4{\pm}2.5$, P < 0.01) as compared to group D patients. The BDI score showed a close correlation with employment status and the life satisfaction scale. Conclusions: Many lumbar spinal stenosis patients had high BDI scores. Employment status and the life satisfaction scale were closely correlated with the BDI score.

A Basic Study of Verbs List for Vocabulary Learning Based on Augmented Reality (증강현실 기반 어휘 지도에서 동사 목록에 대한 기초 연구)

  • Hwang, BoMyung;Kwon, SoonBok;Kim, SeonJong;Shin, BeomJoo
    • 재활복지
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    • v.21 no.2
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    • pp.233-246
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    • 2017
  • The present study is a basic study for application of Augmented Reality (AR) to verb teaching for children with language developmental disorders and is intended to examine validity for the list of verbs at the beginning of development. To confirm the validity of the verbs list, the appropriateness of the verbs was evaluated by three professors with certification of KSLP (Korean Speech-Language Pathologist) working in the department of Speech-Language Pathology at the university. The motion validity test was conducted by showing motion implemented as AR to eight master's students in Speech-Language Pathology major, having them record verbs that came to their mind, and evaluating in the conformity. The second motion validity test was conducted by using 5-point Likert scales to 87 undergraduates in Speech-Language Pathology major and having them see the motions in AR and marked the degrees to which them see the motions conform to the relevant verbs on the scales. Using the SPSS 21.0 program, descriptive statics analyses of the results were conducted. Through this all process, thirty verbs were selected as having content validity. It could be seen that when AR based communication system are applied, things and backgrounds that complement the insufficient movements of motions and help motion recognition should be also provided. In future studies, the 3D images of the AR based communication system will be complemented and the content validity will be verified with typically developing children and the children with language developmental disorders.

Fundamental study on sound absorption of a dental hand piece using micro-porous EPP substrate processed by UV laser (UV 레이저응용 마이크로 다공성 EPP 기판의 치과용 핸드피스 흡음성능에 관한 기초연구)

  • You, Dong-Bin;Shin, Myung-Ho;Byun, Hyo-Jin;Choi, Do-Jung;Sung, Kuo-Won;Ma, Yong-Won;Shin, Bo-Sung
    • Journal of Convergence for Information Technology
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    • v.9 no.5
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    • pp.158-164
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    • 2019
  • Recently many studies to reduce the noise of dental hand piece which generate inevitably mechanical sound to offend to the ear of a patient have been spotlighted. Generally, methods of adding a sound absorbing material inside the exhaust valve, air pump of machine or automobile are widely reported as optimal way to reduce the mechanical noise. In this paper we studied a new UV laser aided manufacturing of micro-porous structure of EPP substrate and applied dental hand piece to improve the efficiency of sound absorption. A lot of micro-sized pores were fabricated with UV laser processing on the surface of sliced EPP substrate. From fundamental experiments, more high-performance of micro-porous EPP substrate has finally demonstrated for sound-absorbing structure of the micro muffler inside dental hand piece, which actually has the excellent potential to apply a lot of potable machine.

In-vitro Neuroprotective Effect of Aricumin(Turmeric extract) (아리큐민의 In-vitro 신경보호 효과)

  • Yoon, Nam kyu;Kim, Byung Kwon;Ryu, Hyeon yeol;Seo, Bo Seung;Shin, Chang Ho;Kim, Kwan Kyu;Lee, Han Joo
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.3
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    • pp.291-296
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    • 2022
  • This study was conducted on curcumin which had increased bioavailability as a potential AChE inhibitor for the treatment of neurodegenerative diseases. The purpose of this study is to confirm the in vitro neuroprotective effect on Aricumin (turmeric extract). To confirm the neuroprotective effect, AChE inhibition for Aricumin was evaluated, and cell viability was analyzed for HT-22cell, and oxidative stress (glutamate, H2O2)-induced HT-22 cytotoxicity was evaluated. As a result of the change in the AChE inhibition rate of Aricumin (Turmeric extract), it was confirmed that Aricumin at a concentration of 39.06㎍/ml or higher inhibited AChE activity by about 20% and more. And it was confirmed that the cytotoxicity of HT-22 cells induced by oxidative stress (Gluamate 5 mM and H2O2 500 µM) was significantly inhibited from 0.01 to 0.1 mg/ml concentration (p<005). These results suggest that Aricumin (turmeric extract) have potential neuroprotective effects.