• Archives of Pharmacal Research
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• v.25 no.4
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• pp.505-510
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• 2002

An improved kinetic assay for prekallikrein activator (PKA), a potential vasodilator, has been developed to be used as an indicator for quality control during production of human albumin preparations. It consists of two reaction stages. In the first stage, PKA and prekallikrein are incubated at $37^{\circ}C$ for 45 min to allow the transformation into kallikrein. Kallikrein, a serine protease, catalyzes the splitting of p-nitroaniline (pNA) from its substrate H-D-Pro-Phe-Arg-pNA(S-2302). The rate at which pNA is released was measured spectrophotometrically at 405 nm. Prekallikrein, a substrate of PKA was purified by DEAE ion-exchange chromatography and the major potential variations in the assay were optimized; pH 8.0 and 150 mM sodium chloride were chosen to give a proper ionic strength. Reaction times in the range of 10 to 360 min provided linear dose-response curves. The concentration of prekallikrein was adjusted to fall between 1:1 and 1:3 dilutions to generate a linear standard calibration curve. Under the optimized conditions, reproducibility was checked. In a precision test, the coefficient of variation (CV) stayed within ${\pm}4%$ and the dose-response curve showed a good correlation (${r^2}=0.999$). An accuracy test with an international standard of PKA afforded a mean recovery of 97.5%.

• Journal of Radiation Industry
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• v.5 no.1
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• pp.47-54
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• 2011

In this study, we investigated to evaluate differential expression of genes encoding lipid transfer proteins (LTP) by acute and chronic gamma irradiation in rice. After acute and chronic gamma irradiation by 100 Gy and 400 Gy to rice plant, necrotic lesion was observed in the leaf blade and anthocyanin contents were increased. We isolated a total of 21 rice lipid transfer protein (LTP) genes in the TIGR database, and these genes were divided into four different groups on the basis of nucleotide sequences. The LTP genes also were classified as different four classes according to expression pattern using RT-PCR. Group A, B contained genes with increased expression and decreased expression in acute and chronic, respectively. Group C contained genes with contrasted expression pattern. Group D wasn't a regular pattern. But the specific affinity was not obtained between two grouping.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.6
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• pp.666-670
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• 2015

Competition among anion species in solution for same sorption sites and soil surface can be of major significance in determining the effective mobility of any potentially adsorbing species. Major soil anion species include $OH^-$, $F^-$, $Cl^-$, $HCO_3{^-}$, $CO_3{^-}$, $NO_3{^-}$, $SO_4{^{2-}}$, and $PO_4{^{3-}}$. And some micro nutrients such as boron and molybdenum exist as $H_2BO_3{^-}$ and $MoO_4{^{2-}}$, as do some heavy metals such as chrome and arsenic as $CrO_4{^{2-}}$ and $HAsO_4{^{2-}}$. Pesticides such as 2,4,5-T and 2,4-D also exist as anions. Many anion species are retained by more complex mechanisms than the simple electrostatic attractions involved in most cation adsorption reactions. In binary system composed of two anions, the adsorption of one anion is influenced by the other anion due to the competition for the available and limited binding sites in soil constituents. The specifically adsorbed anions may compete more effectively for sorption sites than that of nonspecifically adsorbed anion. In this study, we aim to evaluate the mathematical models to determine the magnitude of concentration variations in adsorption due to competitive interactions between anions introduced to a system in binary mixtures.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.2
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• pp.125-134
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• 2023

The swimming behavior of pacific bluefin tuna (Thunnus orientalis) in the offshore sea cage of the brass fishing net was observed and analyzed by imaging sonar techniques. The cultured fish spent most of the time swimming a circular path along the circular cage wall and continued to swim only clockwise direction without completely changing the swimming direction during the 23-hour observation time. In addition, changed swimming behaviors were divided into four categories: (a) the behavior of a large group temporarily swimming in the opposite (counter clockwise) direction, (b) the behavior of a small group temporarily swimming in a small circular path, (c) the behavior swimming small circular path in the center of the cage, and (d) the behavior of a large group swimming across the center of the cage. The maximum swimming speed of the cultured fish was from 3.5 to 3.8 TL/s, the mode was from 1.2 to 1.4 TL/s and the swimming speed during the day time was faster than at night time. It was confirmed the cultured fish swam not only on the surface but also near the bottom net of the cage during the day, but swam mainly at the upper part of the cage at night.

• Korean Journal of Veterinary Service
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• v.46 no.4
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• pp.315-324
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• 2023

This study was carried out to investigate the genotypic diversity of PCV2 and co-infection of PCV3 in the hilar lymph nodes of 700 randomly-selected slaughter pigs. Fourteen samples per each farm were obtained from 50 farms between February and August in 2022. Of the 50 farms, 44 farms that had been positive for PCV2 by RT-PCR were genotyped. As a result of PCV2 genotyping, positive rate of PCV2 DNA was 62.3% (436/700). Among the PCV2 DNA-positive samples, positive rate of a single PCV2 genotype was 79.1% (345/436), while multiple PCV2 genotypes were only detected in 20.9% (91/436). Of the 436 single infection cases, PCV2d genotype was most prevalent. Positive rates of PCV2 and PCV3 were 53.6% and 26.0% at the sample level, 5.1% and 8.0% at the farm level, respectively. And the co-positive rate of two viruses was 8.7% (61/700) at the sample level, 62.0% (31/50) at the farm level. These results demonstrate that PCV2 prevalence in slaughter pigs is very high and co-infection between different PCV2 genotypes and between PCV2 and PCV3 is relatively common. Therefore, genetic diversity and co-infection between other porcine circoviruses should be consistently monitored in the future.

• Korean Journal of Environmental Agriculture
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• v.22 no.2
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• pp.79-86
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• 2003

This research was conducted to present reference data to be used as newly reclaimed tidal land management. We investigated vegetation succession at 4 reclaimed/reclaiming project areas and discussed relationship with soil and vegetation trhrough investigation and analysis soil chemical characteristics at 2 areas. 14 families 58 kinds were investigated. Vegetation were variou at Dea-Ho conservation polt and Seok-Mun National Industrial Area which are maintaining naturally. Vegetation were simple at Hong-Bo and Dongjin and MinKyong river areas which effected sea water. Common species that were investigated at 9 sites were Suaeda asparagoides, Aster tripolium, Phragmites australis, Suaeda maritima, Suaeda japonica, Carex scabrifolis. As soil desalinization progressing, soil classified at first saline-soidc soil, the nest saline soil and then normal soil. Chenopodiaceae revealed at about 30 dS/m of soil ECe and existed to 10 dS/m of soil ECe. At about 20 dS/m of soil ECe. Aster tripolium, Calamagrostis epigeios, and Sonchus brachyotus revealed and then non-halophytes and common plants at inland revealed at low soil ECe of about 10 dS/m. However it was not to progress vegetation sucdession and soil desalinization at the same time, owing to input of seeds or plants ect from out-ecosystem. So for promotion of vegetation at newly reclaimed tidal land, we proposed that it was very effective to plant artificially halophytes or suitable species through soil test.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.2
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• pp.122-128
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• 2001

In order to find out the effect of nitrogen fertilizers on soil pH, EC, $NO_3-N$ and lettuce growth, this study was conducted by pot experiment in plastic film house condition. The square-pot which was $42{\times}54.5{\times}22cm$ in length, width and height, respectively, was filled with two kinds of soils in different soil EC as $0.20dS\;m^{-1}$ and $1.13dS\;m^{-1}$. Seven kinds of nitrogen fertilizers (urea, potassium nitrate, calcium nitrate, ammonium nitrate, ammonium sulphate, complex fertilizer A(11-10-10) and complex fertilizer B(12-12-12)) were treated in same standard rate of nitrogen for lettuce, transplanted the six lettuce seedlings of 10 days grown per pot, and have been grown for 38~44 days with three times harvesting. Soil pH was increased with the potassium and calcium nitrate treatments and decreased with ammonium nitrate, ammonium sulphate, complex fertilzer A and B, and the pH of urea treatments was kept the same value as the pH of before experiment. The growing status of lettuce seedling were surveyed during the early period after transplanting and withering of seedling was occured in all treatments. The withering rates were 10% in soil of EC $0.20dS\;m^{-1}$ and 44% and 42% in complex fertilizer and ammonium sulphate treaments, respectively, in soil of EC $1.13dS\;m^{-1}$. $NO{_3}^-$ contents of lettuce were about $1,000{\sim}2,000mg\;kg^{-1}$ based on fresh weight and these contents were considered to be lower to compare the $NO{_3}^-$ level of EU countries.

• Journal of Nutrition and Health
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• v.51 no.6
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• pp.556-566
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• 2018

Purpose: This study was designed to determine the relationship between health behaviors and high levels of low-density lipoprotein-cholesterol (LDL-cholesterol) according to cardiovascular risk factors among Korean adults. Methods: This cross-sectional study was based on the sixth Korea national health and nutrition examination survey (KNHANES VI). Participants were 13,841 adults aged 19 years and older. Cardiovascular risk factors were stroke, myocardial infarction or angina, diabetes mellitus, smoking, hypertension, aging, high density lipoprotein-cholesterol (HDL-cholesterol) under 40 mg/dL and HDL-cholesterol over 60 mg/dL. Cardiovascular risk groups were classified as very high risk (stroke, myocardial infarction or angina), high risk (diabetes mellitus), moderate risk (over 2 risk factors), and low risk (below 1 risk factor). The prevalence of high LDL-cholesterol was calculated using the LDL-cholesterol target level according to cardiovascular risk group. Results: The prevalence of high LDL-cholesterol was 25.5% in males and 21.7% in females. Complex sample cross tabulation demonstrated that the high LDL-cholesterol and normal groups differed significantly according to age, education, body mass index, percentage of energy from carbohydrate, fat, saturated fat and n-6 in males and females. These two groups were also significantly different according to smoking in males and the percentage of energy from n-3 in females. Complex sample multiple logistic regression analysis adjusted for multiple confounding factors demonstrated that the probability of high LDL-cholesterol was significantly associated with current smoking (OR: 1.66, 95% CI: 1.40-1.99), obesity (OR: 1.95, 95% CI: 1.64-2.31) in males, and current smoking (OR: 1.73, 95% CI: 1.19-2.52), obesity (OR: 1.63, 95% CI: 1.39-1.90), percentage of energy from n-3 (quartile 1 vs. quartile 2; OR: 0.77, 95% CI: 0.62-0.96; quartile 1 vs. quartile 3; OR: 0.73, 95% CI: 0.56-0.94; quartile 1 vs. quartile 4: OR: 0.67, 95% CI: 0.51-0.87) in females. Conclusion: This study reveals the impact of smoking, obesity, energy percentage of nutrient intake on LDL-cholesterol.

• Journal of Life Science
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• v.27 no.1
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• pp.95-99
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• 2017

A previous study demonstrated that four kinds of natural compounds, Corydaline, (${\pm}$)-Car-3-ene-2,5-dione, cinobufagin, and corilagin, enhanced the gene expression of ERAP1 and FOXO1 (DFA16) more than two-fold in a cell culture system. In this study, the experimental food was made finally 30 ml in which included 1% agar, 5% sucrose, and each natural compound $20{\mu}l$. A fruit fly of Drosophila melanogaster fed a natural product for 4 hr after 4 hr starvation. Both natural compounds of Cinobufagin and Corilagin induced 6-8 days more survival comparing than it controls group. The resulting fruit flies were estimated the gene expression of ERAP1 and FOXO1 by RT-PCR that also demonstrated meaningful results with the same lifespan results. Cinobufagin from BufonisVenennumis has $C_{26}H_{34}O_6$ molecular formula and 442 kDa molecular weight. Corilagin from Euphorbiapekinensisis has $C_{27}H_{22}O_{18}$ molecular formula and 634 kDa molecular weight. The two types of natural products screened in this study will be used in the early diagnosis and treatment of insect industry in the near future. In addition, the natural products will be used in longevity experiments in a mouse model. The results may give one of the clues for studying new drug development candidates of the longevity.

• Environmental Engineering Research
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• v.10 no.5
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• pp.213-226
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• 2005

To treat wastewater efficiently by a one-step process of nitrogen removal, a new bacterial strain producing $N_2$ gas from ${NH_4}^+$ under an aerobic condition was isolated and identified. The cell was motile and a Gram-negative rod, and usually occurred in pairs. By 16S-rDNA analysis, the isolated strain was identified as Enterobacter asburiae with 96% similarity. The isolate showed that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. Thus, the consumption of ${NH_4}^+$ by the isolate was significantly different in the metabolism of $N_2$ production under the two different environmental conditions. The optimal conditions of the immobilized isolate for $N_2$ production were found to be pH 7.0, $30^{\circ}C$ and C/N ratio 5, respectively. Under all the optimum reaction conditions, $N_2$ production by the immobilized isolate resulted in reduction of ORP with both the consumption of DO and the drop of pH. The removal efficiencies of $COD_{Cr}$, and TN were 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$, and TN were the highest for the first 2.5 hrs with the removal $COD_{Cr}/TN$ ratios of 32.1, and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous operation was executed with a synthetic medium of a low C/N ratio. The continuous bioreactor system exhibited a satisfactory performance at 12.1 hrs of HRT, in which the effluent concentrations of ${NH_4}^+$-N was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of ${NH_4}^+$-N reached 1.6 mg ${NH_4}^+$-N/L/hr at 12.1 hrs of HRT(with N loading rate of $0.08\;Kg-N/m^3$-carrier/d). As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.