• The Korea Journal of Herbology
• /
• v.29 no.1
• /
• pp.45-52
• /
• 2014

Objectives : The aim of this study was to investigate the effects water extract of fermented new korean medicinal mixture, combinations of Mori Folium, Adenophorae Radix, Phllostachyos Folium and Citri Pericarpium (F-MAPC), on adipocyte differentiation, adipogenesis and glucose uptake using undiffernentiated 3T3-L1 adipocytes and L6 myoblasts. Methods : Each herb and those mixture were respectively fermented and then extracted with water. We carried on MTT assay for check-up on cell toxicity, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ and Glut-4 protein expressions were performed. Results : F-MAPC showed significant inhibitory activity on adipocyte differentiation in 3T3-L1 preadipocytes without affecting cell toxicity as assessed by measuring fat accumulation, and this effect was 2 fold higher in 0.2 mg/ml F-MAPC than that of the same dose of each fermented herbal extract alone. In addition, these effects were associated with modulation of adipogenic transcription factors, such as $C/EBP{\alpha}$, $PPAR{\gamma}$, as well as stimulated phosphorylations of AMPK and ACC. Translocation of Glut-4 was significantly increased by 10.2% in L6 cells treated with 0.2 mg/ml F-MAPC compared with that of control. Conclusions : These results demonstrate that F-MAPC may be an ideal candidate for therapy of obesity and diabetes by disturbing the differentiation into adipocytes, as well as the inducement of intramuscular glucose uptake from blood.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.363-363
• /
• 2022

LID 시설에 대한 모니터링은 인력을 활용한 실강우 모니터링을 진행하고 있으나 LID 시설은 소규모 분산형시설로서 인력을 동원한 식생고사, 강우시 모니터링, 현장답사 등 꾸준한 시설확인에 한계가 있으며, LID 시설을 조성한 이후 적정한 유지관리 방법(주기, 빈도, 항목 등)을 인지하지 못하여 막힘현상, 효율저하, 식물고사 등의 문제가 발생한다. 따라서 본연구에서는 딥러닝 분석을 활용하여 강우시 강우모니터링 자료와 LID 시설 내 센서를 통해 측정된 자료를 통해 침투도랑 내 유입수 성상에 대한 예측분석을 수행하였다. 심지 내 LID 시설에 유입되는 오염물질을 예측을 위한 딥러닝 분석을 위해 과거 실강우시 모니터링 자료(TSS, COD, TN, TP)와 대기센서(대기습도, 대기온도, 강수량, 미세먼지) 데이터를 활용하여 딥러닝 모델에 대한 적용가능성 평가를 수행하였다. 측정항목에 대한 상관성 분석을 수행하였으며, 딥러닝 모델은 Tenser Flow를 이용하여 DNN(Deep Neural Network)모델을 활용하여 분석하였다. DNN 모델에 대한 MSE값은 0.31로 분석되었으며, TSS에 대한 평균 50.6mg/L로 분석되었으며, COD 평균 98.7 mg/L로 나타났다. TN의 평균 2.21 mg/L로 분석되었으며, TP 평균 0.67 mg/L로 나타났다. 상관계수분석결과 TSS는 0.53로 분석되었으며, TN과 TP의 상관계수는 0.10, 0.56으로 나타났다. COD의 상관계수는 0.63으로 TSS와 COD, TP에 대한 예측이 된 것으로 분석되었다. 딥러닝을 통한 LID 시설 내 농도변화 예측시 강우시 센서데이터 값은 조밀해야하며 오염물질 농도와 상관성이 높은 항목들에 대해 계측과 실강우 모니터링 자료를 축적하여 미래에 대한 활용성을 높여야 한다.

• The Korea Journal of Herbology
• /
• v.29 no.4
• /
• pp.77-82
• /
• 2014

Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.453-453
• /
• 2021

도시지역 비점오염원관리, 물순환 회복, 침투 및 증발산량 증가, 열섬현상 저감을 위한 주요한 방안으로 저영향개발(low impact development, LID)과 그린인프라 기법의 적용되고 있다. LID 시설은 소규모 분산형 시설로써 넓은 지역에 많고 다양한 시설들이 적용되어 시설의 개수가 많으며, 수질 및 토양 내 기성제품에 대한 센서들의 가격은 고가로 형성되어 있어 기기의 경제성 및 유지관리 등 적용하는데 제한적이다. 따라서 과거 모니터링 자료를 기반으로 오염물질들과의 상관성 분석을 통하여 계측이 어려운 항목들을 계측가능한 항목들로부터 예측 가능하며, 선정된 항목들에 대한 비용효율적인 센서를 개발하여 실시간 LID 모니터링이 가능한 비용효율적 모니터링을 개발하였다. 공주대학교 천안캠퍼스의 LID 시설들은 2013년에 조성되어 현재까지 시설이 운영되고 있으며, 5년이상의 과거 강우시 모니터링 자료들을 이용하여 오염물질 상관성 분석을 수행가능 하기에 대상지로 선정하였다. 오염물질 상관성 분석은 2013년부터 2017년도에 침투도랑에서 수행된 강우시 모니터링 자료를 활용하여 각 오염물질들의 상관성을 분석을 수행하였다. 침투도랑 내 유입되는 평균 유입수는 TSS 286.1±318.3 mg/L, BOD 22.6±39.5 mg/L, TN 8.96±5.85 mg/L, TP 1.01±1.11 mg/L로 나타났다. 겨울철에 비해 여름철에서의 오염물질의 유입농도가 높은 것으로 분석되었다. 이는 여름철 고온건조로 인한 노면 내 차량의 주행으로 인한 중금속, 폐타이어 등과 장마철 강우 시 유출된 토사로 인하여 유입수의 농도가 높은 것으로 분석되었다. 오염물질 부하량은 TSS와 COD 0.66으로 유의성이 높은 것으로 나왔으며, COD와 TSS, TP, TN 등 유의성이 높은 것으로 분석되었다. Arduino와 Raspberry PI를 활용하여 저비용 센서와 LTE 모뎀통신과 데이터 베이스 연결하여 개발된 프로그램을 통해서 무선으로 LID 시설에 대한 모니터링을 침투화분2와 식생체류지에 조성하였다. 전력공급이 어려운 식생체류지의 경우 태양열(Solar system) 시스템과 보조 전력 배터리를 조성하여 장마철이나 장기적인 악천후로 인한 전력을 생산하지 못할 경우 보조전력배터리에서 전력을 제공하여 지속적인 모니터링이 이루어지도록 설계하였다. 토양함수량, 토양온도와 Conductivity 등 3종류의 센서를 적용하였으며, 프로그램은 현재 2단계를 통한 2차수정을 통하여 프로그램을 구축하였다. 오차, 오작동, 계측값에 대한 검·보정 작업이 필요하다. 또한 대기자료의 구축을 통해 보다 토양과 LID 시설에 대한 영향분석이 필요한 것으로 사료된다.

• Korean Journal of Remote Sensing
• /
• v.25 no.2
• /
• pp.193-203
• /
• 2009

Snow cover is one of the important parameters since it determines surface energy balance and its variation. To classify snow and cloud from satellite data is very important process when inferring land surface information. Generally, misclassified cloud and snow pixel can lead directly to error factor for retrieval of surface products from satellite data. Therefore, in this study, we perform algorithm for detecting snow cover area with remote sensing data. We just utilize visible reflectance, and infrared channels rather than using NDSI (Normalized Difference Snow Index) which is one of optimized methods to detect snow cover. Because COMS MI (Meteorological Imager) channels doesn't include near infra-red, which is used to produce NDSI. Detecting snow cover with visible channel is well performed over clear sky area, but it is difficult to discriminate snow cover from mixed cloudy pixels. To improve those detecting abilities, brightness temperature difference (BTD) between 11 and 3.7 is used for snow detection. BTD method shows improved results than using only visible channel.

• The Korea Journal of Herbology
• /
• v.29 no.2
• /
• pp.15-21
• /
• 2014

Objectives : The purpose of this study was to investigate inhibitory effects of steamed Polygonatum odoratum extract (POE) on differentiation and adipogenesis in 3T3-L1 adipocytes. Methods : Polygonatum odoratum (P. odoratum) extract was extracted with ethyl acetate. Total phenolic and flavonoid contents in POE were measured for antioxidant activity. The spectrophotometric method was used to determine the DPPH and ABTS radical scavenging activity and ferric-reducing antioxidant potential (FRAP). MTT assay was examined for cell toxicity, oil red O staining was performed for intracelluar adipogenesis in differentiated 3T3-L1 adipocytes. Western blot analysis for measurement of CCAAT/enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$), peroxisome proliferator-activated receptor${\gamma}$ ($PPAR{\gamma}$) and AMP-activated protein kinase (AMPK) expressions were performed. Results : The results revealed that POE has antioxidant activities. Contents of total polyphenolics and flavonoids were $50.83{\pm}1.52$ GAE mg/100g dry weight of POE and $17.05{\pm}2.47$ RE mg/100g dry weight of POE, respectively. DPPH radical scavenging activity, and FRAP in 10 mg/ml concentration were $92.1{\pm}0.6%$, $244.8{\pm}9.0{\mu}M$ Fe(II) and ABTS inhibition in 5 mg/ml concentration was $84.8{\pm}4.1%$. Treatment of POE in adipocytes inhibited the differentiation and adipogenesis of 3T3-L1 adipocytes compared to those of vehicle control. Additionally, protein expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, major transcription factor for the adipogenic genes, were significantly decreased compared to those of vehicle control (p<0.05). Futhermore, phosphorylation of AMPK was increased in 3T3-L1 adipocytes treated with POE compared to that of vehicle control (p<0.05). Conclusions : we demonstrate that steamed P. odoratum extract (POE) has potentiating antioxidant activities, inhibits differentiation and lipid accumulation and also induces energy expenditure in adipocytes, which may contribute to antiobesity property.

• Journal for History of Mathematics
• /
• v.21 no.1
• /
• pp.79-96
• /
• 2008

In early 21st century, universities in Korea has been asked the new roles according to the changes of educational and social environment. With Korea's NURI and Brain Korea 21 project support, some chosen research oriented universities now should produce "teacher of teachers". We look 100 years back America's mathematics and see many resemblances between the status of US mathematics at that time and the current status of Korean mathematics, and find some answer for that. E. H. Moore had produced many good research mathematicians through his laboratory teaching techniques. R. L. Moore was his third PhD students. He developed his Texas/Moore method. In this article, we analyze what R. L. Moore had done through his American School of Topology and Moore method. We consider the meaning that early University of Texas case gives us in PBL(Problem Based Learning) process.

• Journal of agriculture & life science
• /
• v.45 no.6
• /
• pp.163-173
• /
• 2011

The objective of this study was to determine microbiological risk factors in hot pepper farms for the application of good agricultural practices (GAP). Samples were collected from cultivation environments and utensils, plants, workers, and air at 3 hot pepper farms located in Cheongsong, Korea and were tested to detect sanitary indications [aerobic plate bacteria (APC), coliform, and Escherichia coli], foodborne pathogens, and fungi. APC, coliform, and fungi were detected at the levels of 0.7~6.2, 0.2~4.7, and 0.4~4.3 log CFU, respectively, in the three farms. Four (4.4%; l leaf, l irrigation water, and 2 soil) of 90 samples collected were revealed to be E. coli positives. For foodborne pathogens, Staphylococcus aureus was only detected at $1.0log\;CFU/100cm^2$ in the worker's cloth of B farm, and Bacillus cereus was detected at the levels 1.0~2.5 log CFU in the cultivation environments and utensils and worker of B and C farms. However, other pathogens were not detected. The results demonstrated potential microbiological risks for hot pepper cultivated in the farms. Therefore, a management system to minimize the microbial risk such as GAP is required to ensure the safety of hot pepper.

• Journal of Life Science
• /
• v.28 no.12
• /
• pp.1523-1528
• /
• 2018

This study investigated the chemical composition of four Korean cultivated Chrysanthemum indicum L. (Gamguk 1 ho, Gamguk 2 ho, Gamguk 3 ho, and Wonhyang) which are used in the food and fragrance industries to identify their volatile flavor compounds. These compounds were analyzed using headspace GC-MS from plant samples cultivated in the same region of Korea (Sancheong-gun, Gyeongsangnam-do). A total of 23 compounds were identified, eight of which were common across the four cultivars. The major flavor components in the three Gamguk plants were identified as 3-carene, camphene, ${\beta}$-phellandrene, eucalyptol and (+)-camphor. Eleven compounds, including (+)-camphor at 31.40%, were identified in Gamguk 1 ho. Gamguk 2 ho was found to contain 12 flavor compounds, predominant of which was camphene at 25.60%. Thirteen compounds including (+)-camphor (26.88%) were identified in Gamguk 3 ho, while 17 were detected in the Wonhyang cultivar, including trans-piperitol (47.33%), sabinene, and ${\gamma}$-terpinyl acetate. These results indicate differences in the type and ratio of functional volatile flavor ingredients in Chrysanthemum indicum L. cultivars which is highly valuable as material for fragrance product development.

• Korean Linguistics
• /
• v.63
• /
• pp.103-132
• /
• 2014

This study compares various speech rates of Korean learners with those of native Korean. Speech data were collected from 34 native Koreans and 33 Korean learners (19 Chinese and 14 Japanese). Each participant recorded a 9 syllabled Korean sentence at three different speech rate types. A total of 603 speech samples were analyzed by speech rate types (normal, slow, and fast), native languages (Korean, Chinese, Japanese), and learners' proficiency levels (beginner, intermediate, and advanced). We found that learners' L1 background plays a role in categorizing different speech rates in the L2 (Korean), and also that the leaners' proficiency correlates with the increase of speaking rate regardless of speech rate categories. More importantly, faster speech rate values found in the advanced level of learners do not necessarily match to the native speakers' speech rate categories. This means that learning speech rate categories can be more complex than we think of proficiency or fluency. That is, speech rate categories may not be acquired automatically during the course of second language learning, and implicit or explicit exposures to various rate types are necessary for second language learners to acquire a high level of communicative skills including speech rate variation. This paper discusses several pedagogical implications in terms of teaching pronunciation to second language learners.