• Korean Journal of Fisheries and Aquatic Sciences
• /
• 제47권5호
• /
• pp.501-507
• /
• 2014

Norovirus (NoV) is a major cause of food poisoning outbreaks in Korea. Most NoV outbreaks originate from environmental contamination, but bivalves such as oysters are also important vectors. Oyster Crassostrea gigas contamination by NoV has been reported in Korea, but no quantitative analyses of NoV have been performed. We investigated the NoV concentration in 21 oyster samples from a Korean commercial oyster-growing area with confirmed fecal contamination from January to December 2012, using real-time reverse transcription-polymerase chain reaction. Additionally, we assessed the NoV concentration after heating to investigate the effects of heat treatment on NoV-infected oysters. In NoV-positive samples, the cycle threshold (Ct) values were 37.43-39.41 and 36.77-39.30, while viral concentrations were $8.97{\times}10^2-2.24{\times}10^2$ and $3.05{\times}10^2-7.47{\times}10^1$ copies/g for genogroups I and II, respectively. After heat treatment, NoV genogroup I decreased by 83.4%, 88.0%, 89.4% and 100% at $60^{\circ}C$, $68^{\circ}C$, $70^{\circ}C$, and $100^{\circ}C$, respectively, for 15 min, while genogroup II respectively decreased by 67.3%, 76.3%, 80.1%, and 89.8% under the same conditions.

• Environmental Analysis Health and Toxicology
• /
• 제21권2호
• /
• pp.115-126
• /
• 2006

This study was conducted to find out survival rate, activity, growth and change of the organ structure of bivalves exposed to heavy metal. The results of the study confirmed that zinc (Zn) induces reduction of survival rate and activity, abnormality of organ structure of the equilateral venus, Gomphina veneriformis. Experimental groups were composed of one control condition and three Zn exposure conditions ($0.64mg\;Zn{\iota}^{1},\;1.07mg\;Zn{\iota}^{-1},\;1.79mg\;Zn{\iota}^{-1}$). As the concentration of zinc increased the accumulation of lipofucin increased in the digestive gland. Survival rate was the lowest in the lowest Zn exposure group at $0.64mg;Zn{\iota}^{-1}$. Growth was not significantly different between the control and exposure group. Activity. with the exception of the lowest Zn exposure group at $0.64mg\;Zn{\iota}^{-1}$, was similar between the control and exposure group. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis, increase in the number of mucous cell in the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and foot.

• Fisheries and Aquatic Sciences
• /
• 제5권3호
• /
• pp.183-190
• /
• 2002

To determine the effect of body size on the clearance rate and ingestion rate of small intertidal bivalves, Glauconome chinensis, feeding experiments were conducted on individuals of 12 different size classes, from 4 to 16 mm in shell length. Relationships between morphological parameters were also determined. The clearance and ingestion rates of G. chinensis responded similarly to their body size, ranging from 1.3 to 28.2 mL/hr/ind. and from 24.0 to 458.5, ${\mu}gC/hr/ind$., respectively. Both rates increased significantly (p<0.001) as shell length increased from 4 to 9 mm, although neither rate changed significantly when shell length was in the range from 12 to 16 mm. The weight-specific clearance rate $(CR_w)$ and ingestion rate $(IR_w)$ decreased with increasing body size, with values from 1.0 to 3.1 L/hr/g and from 17.9 to 51.3 mgC/hr/g, respectively. The $CR_w$ of G. chinensis was intermediate compared to those of larger bivalve species. The clearance rate (CR) relative to flesh dry weight (FDW) of G. chinensis were fitted well to the power function: $CR=0.43\times(FDW)^{0.71}\;(r^2=0.89)$. The exponent of fitting equation (0.71) of G. chinensis was higher than those of Mytilus edulis (Walne, 1972), Crassostrea gigas (Walne, 1972), and Placopecten magellanicus (MacDonald and Thompson, 1986).

• The Korean Journal of Malacology
• /
• 제18권1호
• /
• pp.1-8
• /
• 2002

Seasonal changes in biochemical composition of Crassostrea giggas and Scapharca broughtonii in the Kosong bay and Kangjin bay, southern Korea were studied over 12 months in relation to their habitat. In Crassostrea giggas, protein was high during summer in all experimental station, but lipid and carbohydrate were high during winter, and no clear difference was between experimental station. Glycogen content was sharply decreased during summer, and energy content was low but carbohydrate was high during summer. In Scapharca broughtonii, protein was low, but carbohydrate was high during summer. Lipid was high from February to March, glycogen content was low from June to September. Energy content was low during winter and no clear difference biochemical composition was between experimental station of Scapharca broughtonii.

• The Korean Journal of Malacology
• /
• 제27권3호
• /
• pp.253-259
• /
• 2011

Boring behaviour and drilling mechanism were investigated by visual observations. In this study, of two kinds of holes (the outer and inner holes) which are formed by drilling of boring gastropod Lunatia fortunei (Naticidae), the diameters of the outer holes are broader and larger than those of the inner holes, and their holes look like the crater in shape, as seen in all valves of bivalves bored by Naticidae species. Two kinds of glands (the accessory boring gland and accessory salivary gland) on the foots of boring gastropods have been investigated. Of them, it has been confirmed that only the accessory salivary glands on the foots secreted sulphuric (acidic) components in the mucus (secretion), while the accessory boring glands on the foots did not secrete their components. In this study, we confirmed that L. fortunei possess the accessory boring gland on the foot, as seen in most species in Naticidae. Accoeding to the results of the experiment of the blue litmus paper tests of the mucus (secretions) secreted from the accessory boring gland the color of the blue litmus paper did not turn red in color because chemical components of mucus (secretion) secreted from the accessory boring gland on the foot of L. fortunei (boring gastropod) were not acidic components. It is supposed that the mucus, which is secreted from the accessory boring gland, contained gelatin-like substances or enzymes without acidic components, as already reported in Naticidae species. Therefore, these substances may be involved in softening the surface of the valves of M. veneriformis. Consequently, it is assumed thar L. fortunei bores holes through the shells of molluscs by means of following 3 methods: (1) a softening of the calcareous shells of M. veneriformis with chemical secretions (including gellatin-like substances or enzyme except for acidic components) from the accessory boring glands, (2) mechanical rasping with the radula, (3) a combination of both. In this study, particularly, acidic components, which are involved in softening the surface of the shells, are not associated with the boring mechanism of L. fortunei because chemical acidic components were not detected in the mucus (secretion), as found in Naticidae species.

• The Korean Journal of Malacology
• /
• 제28권3호
• /
• pp.277-291
• /
• 2012

Aquaculture is challenged by a number of constraints with future efforts towards sustainable production. Global climate change has a potential damage to the sustainability by changing environmental surroundings unfavorably. The damaging parameters identified are water temperature, sea level, surface physical energy, precipitation, solar radiation, ocean acidification, and so on. Of them, temperature, mostly temperature elevation, occupies significant concern among marine ecologists and aquaculturists. Ocean acidification particularly draws shellfish aquaculturists' attention as it alters the marine chemistry, shifting the equilibrium towards more dissolved CO2 and hydrogen ions ($H^+$) and thus influencing signaling pathways on shell formation, immune system, and other biological processes. Temperature elevation by climate change is of double-sidedness: it can be an opportunistic parameter besides being a generally known damaging parameter in aquaculture. It can provide better environments for faster and longer growth for aquaculture species. It is also somehow advantageous for alleviation of aquaculture expansion pressure in a given location by opening a gate for new species and aquaculture zone expansion northward in the northern hemisphere, otherwise unavailable due to temperature limit. But in the science of climate change, the ways of influence on aquaculture are complex and ambiguous, and hence are still hard to identify and quantify. At the same time considerable parts of our knowledge on climate change effects on aquaculture are from the estimates from data of fisheries and agriculture. The consequences may be different from what they really are, particularly in the temperature region. In reality, bivalves and tunicates hung or caged in the longline system are often exposed to temperatures higher than those they encounter in nature, locally driving the farmed shellfish into an upper tolerable temperature extreme. We review recent climate change and following environment changes which can be factors or potential factors affecting shellfish aquaculture production in the temperate region.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• 제1권2호
• /
• pp.115-119
• /
• 1968

1. The basic investigations on soil textures, water holding capacity, pH, exchangeable calcium, and organic matter contents in four species bed were conducted in order to improve the natural flat for the bivalve beds. 2. It was statistically significant that there were differences in the soil textures among all beds of four species, D. japonica, C. sinensis, M. veneriformis, and T. philippin-arum. 3. There was no significant difference in water holding capacity, pH, and exchange-able calcium contents among different bivalve beds statistically, 4. It was highly significant that there were diffences in soil organic matter content among all groups of beds except that of D. japonica - C. sinensis. 5. It is considered that that soil textures and soil organic matter contents are most important factors to restrict the distribution of each species bivalve within a certain limited area of tidelands.

• Fisheries and Aquatic Sciences
• /
• 제12권4호
• /
• pp.276-285
• /
• 2009

Despite great commercial interest, relatively little has been described about molecular mechanism of bivalve reproduction. We investigated genes involved in ovarian maturation of the Yesso scallop, Patinopecten yessoensis. GSI index and histological analysis revealed that maturation of ovary begin in February and spawning period is from April to June which is similar to the previous study in the East Sea. As result of combination analysis of differential display RTPCR (DDRT-PCR) and histological examination, vitellogenin (Vg), ferritin (Ft) and ADT/ATP carrier protein (ACC) were identified as differently expressed genes in maturating ovary. Endpoint RT-PCR results showed that Vg is ovary-specific genes whereas Ft and ACC are expressed ubiquitously suggesting that Vg can be good molecular markers for ovarian development and sex determination in bivalves. Quantitative PCR results revealed that Vg were expressed highest during growth stage and appears to play a major role in oocyte maturation. On the contrary, expression of Ft was highest after spawning stage, which suggests that up-regulation may be involved in spawning and inactive stages in which the scallops recover from spawning. In addition, high level of the mitochondrial gene, ACC, may play a role in energy metabolism in maturating oocytes. Isolation and molecular studies of these key genes will expand our knowledge of the physiological changes from various exogenous factors including temperature, salinity, pH, even or numerous endocrine disrupting chemicals (EDCs) during reproductive cycle. In addition, further study of these genes implicates various industrial applications including the stable seed production, increased food quality, or economic aquaculture system.

• Korean Journal of Ichthyology
• /
• 제24권1호
• /
• pp.35-40
• /
• 2012

The feeding habits of yellowback seabream, $Dentex$ $tumifrons$, were studied using 317 specimens collected in the coastal waters of Busan, from January to December, 2004. The size of the specimens ranged from 10.2 to 27.8 cm in standard length (SL). D. tumifrons had turned out a carnivore and opportunistic predator that consumed mainly shrimps. Fishes were next important prey items. Its diet also included anomurans, amphipods, crabs, echinodermata and cephalopods. Polychaetes, stomatopods, bivalves, ostracods, mysids and so on were minor preys. The individuals of smallest size class (10~13 cm SL) fed mainly on shrimps. In the next size class (13~16 cm SL), the proportion of shrimps decreased, whereas the consumption of anomurans, echinodermata and crabs increased. The proportion of these prey items decreased as body size increased, whereas the consumption of fishes gradually increased. Fishes accounted for almost stomach contents of larger individuals (more than 25 cm SL).

• Korean Journal of Ichthyology
• /
• 제22권1호
• /
• pp.41-48
• /
• 2010

The feeding habits of Chaenogobius gulosus were studied based on an examination of the stomach contents of 333 specimens collected between October 2008 and September 2009 in the coastal waters of Tongyeong, Korea. The specimens ranged in standard length (SL) from 2.0 to 12.6 cm. C. gulosus is an omnivore and consumes mainly seaweeds (such as Ulva pertusas), crabs and gastropods. Its diet also included small quantities of bivalves, polychaetes, insects, shrimps, mysids and amphipods. Smaller individuals (<6 cm SL) consumed mainly gastropods. The proportion of these prey items decreased with increasing fish size, and this decrease paralleled the increased consumption of seaweeds. Seasonal changes in the diet of C. gulosus were significant. Seaweeds were consumed more during spring and summer compare with other seasons.