• Title/Summary/Keyword: BHT effect

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Antioxidant Effect of Extracts Obtained from Three Chrysanthemum Species (국화과 Chrysanthemum속 식물 3종의 항산화 효과)

  • Woo, Jeong-Hyang;Shin, So-Lim;Jeong, Heon-Sang;Lee, Cheol-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.4
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    • pp.631-636
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    • 2010
  • To develop a natural antioxidant from three Chrysanthemum species, flower and shoot extracts of Chrysanthemum frutescens, Chrysanthemum morifolium and Chrysanthemum zawadskii ssp. naktongense were obtained and their phenolic compound contents, scavenging effects on DPPH and ABTS radicals, ferrous ion chelating effects and inhibition activity on lipid peroxidation of linoleic acid were studied. Shoots of C. morifolium showed the highest levels in all above mentioned analyses. Especially, shoot extract of C. morifolium had high scavenging activities on ABTS radicals, similar to ascorbic acid or BHT. Ferrous ion chelating effect was the lowest in a C. morifolium shoot extract, but the highest in a C. morifolium flower extract. Inhibition activity on lipid peroxidation of linoleic acid was the highest with C. frutescens and C. morifolium shoots, but activity was lower than BHT. From present study, a shoot extract of C. morifolium is demonstrated as a valuable source for the development of a natural antioxidant. However, due to its low levels of ferrous ion chelating effects and inhibition activity on lipid peroxidation, a combination of other antioxidants with C. morifolium extract is recommended for the development of a new antioxidant.

Antioxidant Atctivity of Crude GingerolI. Thermal Stability of Gingerol from Ginger and Effect of its Concentration on the Oxidation of Soybean Oil (Crude Gingerol의 항산화 효과 I. 생강 Gingerol의 열안정성 및 대두유에 대한 농도에 따른 항산화 효과)

  • 백숙은;우상규
    • Korean journal of food and cookery science
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    • v.9 no.1
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    • pp.33-36
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    • 1993
  • This study was carried out to investigate the thermal stability of gingerol from Ginger and effect of its concentration on the oxidation of soybean oil. After Heating gingerol, the contents of remained gingerol was measured by HPLC. After heat treatment at $105^{\circ}C$ for 3 hours, 94.5% of gingerol was remained, whereas at $165^{\circ}C$ for 30 min., only 10% of gingerol was remained. Crude gingerol was also added to soybean oil at the concentration of 0.02%, 0.1% and 0.2% by weight of oil, respectively. The oils with crude gingerol were heat-treated at $105^{\circ}C$ for 5 hours and $165^{\circ}C$ for 30 minutes, respectively, and then the heat-treated oils were incubated at $45^{\circ}C$ for 25 days. The peroxide value of the oils was measured in order to estimate the antioxidant activity of crude gingerol compared with BHT. In the soybean oil heated at $105^{\circ}C$ for 5 hours, crude gingerol showed antioxidant activity at all concentration and the activity was more effective than 0.02% BHT. And during the storage of the heat-treated oils at $45^{\circ}C$, the antioxidant activity of crude gingerol increased in direct proportion to its concentration. In the soybean oil heated at $165^{\circ}C$ for 30 minutes and then stored at $45^{\circ}C$, the antioxidant activity of crude gingerol increased in direct proportion to its concentration.

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Antioxidant Effect of Sargassum hemiphyllum Extracts (짝잎모자반(Sargassum hemiphyllum) 추출물의 항산화 효과)

  • Park, Ji-Hye;Park, Sun-Hee;Kim, Min-Ji;Kim, Koth-Bong-Woo-Ri;Choi, Jung-Su;Ahn, Dong-Hyun
    • Microbiology and Biotechnology Letters
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    • v.45 no.2
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    • pp.118-123
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    • 2017
  • This study was conducted to determine the antioxidant activities of ethanol and water extracts of Sargassum hemiphyllum. Antioxidant activities were evaluated by assessing total phenolic contents, 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, chelating effect, reducing power, and using the rancimat method. Total phenolic contents in the ethanol and water extracts were 17.91 and 13.44 mg gallic acid equivalents/g, respectively. Ethanol extract showed higher DPPH radical scavenging activity than water extract and similar activity to that of BHT. The reducing power of ethanol and water extracts increased in a concentration-dependent manner. Particularly, ethanol extract was more effective than water extract. Water extract showed a higher chelating effect compared to ethanol extract. The antioxidant index measured by rancimat was lower than those in BHT, but the ethanol extract showed a higher value than the water extract. The ethanol extract showed higher antioxidant activity than the water extract, except for the chelating effect. These results suggest that the ethanol extract of Sargassum hemiphyllum has more potent antioxidant activity and may be used as a source of natural antioxidants.

The study on thw Inhibitory effect of Allerg by SoPungSan (消風散의 Allerhy 抑制 效能에 관한 硏究)

  • Seo, Hyung-shikk;Roh, Seok-sun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.13 no.1
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    • pp.22-29
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    • 2000
  • This study was carried out to prove the effects of SPS on antioxidants, iching by histamine in human model and skin stimulus. The result were obtained as follows : 1. Antioxidants of SPS is better than Vitamine C and is not less than BHT(Dibutylated Hydroxytoluene). 2. Inhibitory effect of iching by histamine is better than Dermobate. 3. Skin stimulus is not in ROAT(Repeated Open Application Test).

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The Antioxidative Effects of Maillard Reaction Mixtures of Oligosaccharides (올리고당의 Maillard 반응물질의 유지에 대한 항산화효과)

  • Lee, Su-Mi;Ahn, Myung-Soo
    • Journal of the Korean Society of Food Culture
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    • v.12 no.2
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    • pp.195-200
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    • 1997
  • The purposes of this study were to investigate the Maillard reactions of some oligosaccharides with lysine and the antioxidative effects of the ethanol extracts from their reaction mixtures on the soybean oil. The Maillard reactions were carried out of 2% oligosaccharides such as palatinose (PN), fructooligosaccharide (FO), isomaltooligosaccharide (IMO) with 2% lysine (L) for 24 hours heating at 60, 80, $100^{\circ}C$. The color intensity of Maillard reaction mixtures were determined by UV-VIS spectrophotometer upon reaction time and temperature. And the antioxidative effects on the soybean oil of each ethanol extract from Maillard reaction mixture of each oligosaccharide were measured by peroxide value (POV). POV's of soybean oil including reaction extracts were determined regularly every 2 days during 20 days storaged at $60{\pm}1^{\circ}C$. The results were obtained as follows: 1. The color intensity of the Maillard reaction mixtures were raised highly as the browning temperature and time increased. The color intensity of PN L browning mixture was the highest. The order of high color intensity at $100^{\circ}C$ was PN L>FO L>Glu L>IMO L. 2. Comparing the antioxidative effect of Maillard reaction product (at $100^{\circ}C$, for 12 hours) of each oligosaccharide to that of BHT and TBHQ, the order of high antioxidative effect was TBHQ>IMO L>BHT>Glu L>PN L>FO L. 3. From these results, it was known that PN L shown as high brown color intensity was appeared low antioxidative effect, while IMO L shown as low brown color intensity was appeared high antioxidative effect. So, it was recognized that there was no relation between brown color intensity and antioxidative effect.

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The effect of Kami-Saenghwatang and Kami-Bohertang to postpartum weight retention (가미생화탕과 가미보허탕의 연속 투여가 산후 체중정체와 체성분 변화에 미치는 영향)

  • Ha, Jeong-Il;Kim, Dong-Il
    • The Journal of Korean Obstetrics and Gynecology
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    • v.20 no.2
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    • pp.164-175
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    • 2007
  • Purpose: The purpose of this study was to investigate the relationships between the herbal medicines used for women in puerperium on postpartum care, Kami-Saenghwatang(SHT) and Kami-Bohertang(BHT) and postpartum body composition change. We also examined the influence of gestational age, the number of parity and the route of delivery for gestational weight gain and postpartum weight loss. Methods: 239 women followed by postpartum care center. Variety factor s were conformed and then checked body composition analysis. 8 weeks later, we followed up body composition of 36 women among the former checked. and additionally asked the body weight of another 42 former checked women by telephone call. Results: $Mean{\pm}S.D$(standard deviation) of pre-pregnancy weight is $55.22{\pm}8.98kg$, $Mean{\pm}S.D$ of gestational maximun weight is $69.26{\pm}9.77kg$, $Mean{\pm}S.D$ of weight loss in postpartum 8 weeks(means sub-tract postpartum 1 week weight from postpartum 8 week weight) is $57.86{\pm}8.60kg$. The number of parities had corrrelation with gestational weight gain. Age, number of parities and route of delive were not significantly correlated with postpartum weight loss during 8 weeks after delivery, and also it was not correlated with body water mass, body fat mass, body mass index and body weight on postpartum 8 weeks whether they treated with SHT and BHT. conclusion: It was not correlated with body water mass, body fat mass, body mass index and body weight on 8 weeks postpartum whether they treated with SHT and BHT.

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Antioxidative Activities of Castanea Crenata Flos. Methanol Extracts (밤꽃(Castanea Crenata Flos.) 메탄올 추출물의 항산화 효과)

  • Choi, Chang-Suk;Song, En-Sung;Kim, Jang-Su;Kang, Myung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1216-1220
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    • 2003
  • The antioxidative activities of methanol extracts of chestnut flower (Castanea Crenata Flos.) were determinated in vitro using an experimental model system. Solid yield of chestnut flower extracts was 6.26% and total phenolic acid accounted for about 20% of the crude extract. The DPPH radical scavenging activity of methanol extracts prepared from chestnut flower was 17.22%. Although the DPPH radical scavenging activity of chestnut flower extracts was lower than that of other antioxidants, chestnut flower extracts showed continuous activity by time course. The SOD-like activities of methanol extracts prepared from chestnut flower were 65.10%, 95.70% in BHT, 93.29% in quercetin, and 30.30% in ascorbic acid. Chestnut flower extracts showed 51.45% inhibitory effect on peroxidation of egg yolk lecithin.

Antioxidant Activities and Cell Viability against Cancer Cells of Adenophora remotiflora Leaves (모시잎의 항산화 효과 및 암세포주에 대한 세포 독성)

  • Kim, In-Sook;Park, Kwon-Sam;Yu, Hyeon-Hee;Shin, Mee-Kyung
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.3
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    • pp.384-394
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    • 2009
  • This study was performed to determine the antioxidative and anticancer effects of extracts from Adenophora remotiflora leaves. The antioxidative effects of the extracts were measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and hemoglobin-induced linoleic acid oxidative inhibition assays. The results indicated that the extracts had stronger effects than the synthetic antioxidant BHT at the same concentration. The $SC_{50}$ values (50% radical scavenging effect on $1{\times}10^{-4}$ M DPPH) of the methanol fraction, water extract, and BHT were 47.5 ${\mu}g$/mL, 74.6 ${\mu}g$/mL and 102.2 ${\mu}g$/mL, respectively. In addition the $IC_{50}$ values (hemoglobin-induced linoleic acid oxidation inhibition) of the methanol fraction, water extract, and BHT were 120.8 ${\mu}g$/mL, 135.6 ${\mu}g$/mL, and 150.2 ${\mu}g$/mL, respectively. This research also assessed decreases in the survival of BNLcl2 cells (normal liver cells) by solvent fractions of the A. remotiflora leaf extracts at various concentrations (1, 5, 10, 25, 50, 100, 250, 500, 1,000, 2,000 ${\mu}g$/mL). The water extract did not decrease survival at any of the concentrations when compared to the control group. The hexane, ethyl acetate, and methanol fractions decreased survival as compared to the control group by inducing cell toxicity at a concentration of 1,000 ${\mu}g$/mL and above. Therefore, an anticancer activity experiment was conducted using concentrations below 500 ${\mu}g$/mL. At 500 ${\mu}g$/mL, the methanol fraction decreased A549 cell (human lung carcinoma cells) survival by 46% as compared to the control group, presenting the greatest effect against cell survival. All extracts showed greater anticancer activity in Hep G2 cells (human liver carcinoma cells) as compared to the A549 cells. For the Hep G2 cells, the methanol extract decreased survival by 28% as compared to the control group at the concentration of 500 ${\mu}g$/mL, thus restraining lung cancer cell growth.

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Antioxidative Effect of Angelica acutiloba Kitagawa Ethanol Extract (일당귀 에탄올 추출물의 항산화 효과)

  • Kim, Ah-Ra;Lee, Jae-Joon;Lee, Myung-Yul
    • Journal of Life Science
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    • v.19 no.1
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    • pp.117-122
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    • 2009
  • This study was conducted to investigate the antioxidant effect of 80% ethanol extracts from Angelica acutiloba Kitagawa (A. acutiloba Kitagawa) in vitro. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value (POV) in linoleic acid in comparison with the commercial antioxidant butylated hydroxy toluene (BHT). Antioxidant activities of n-hexane fraction of Angelico acutiloba Kitagawa ethanol extract were the highest among fractions and were a little less than that of BHT. Nitrite scavenging activity showed the most remarkable effect at pH 12. These results suggest that ethanol extracts of A. acutiloba Kitagawa can be used in natural antioxidant source.

Antioxidative Effect of Chaenomelis Fructus Ethanol Extract (모과 에탄올 추출물의 항산화 효과)

  • Lee, Yu-Mi;Shin, Hyoung-Duck;Lee, Jae-Joon;Lee, Myung-Yul
    • Food Science and Preservation
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    • v.14 no.2
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    • pp.177-182
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    • 2007
  • This study was performed to investigate the antioxidant effect of 80% (v/v) ethanol extract from Chaenomelis Fructus (CF). Total flavonoids and total polyphenols in the extract were also measured spectrophotometrically. The extraction yield was 9.23g/100g CF. The extract was further fractionated by partition with n-hexane, chloroform, ethylacetate, butanol, and water. The water fraction showed the highest extraction yield of all fractions. The n-hexane method and compared with the properties of the commerical antioxidant BHT. The activities of the n-hexane fraction were the highest of all fractions. In addition, there was strong positive correlation between antioxidant activities and levels of antioxidative compounds, such as flavonoid and polyphenols, in CF fractions, suggesting that these antioxidative compounds may contribute to the antioxidative effect of CF.