• Journal of Animal Science and Technology
• /
• 제51권5호
• /
• pp.407-412
• /
• 2009

본 연구는 송아지 설사병의 주요원인체 중 소로타바이러스(bovine rotavirus; BRV)와 소코로나바이러스(bovine coronavirus; BCV)에 대한 난황항체를 생산하고 이의 면역 특이성을 확인하고자 실험을 실시하였으며, BRV 및 BCV를 2주 간격으로 5회 산란계에 근육주사를 실시하여 혈청과 난황내의 특이 항체 형성 유무를 확인하였다. 실험 6주차에 면역한 산란계로부터 획득한 혈청을 이용하여 교차반응 시험을 실시한 결과, BRV 및 BCV를 면역하여 얻은 혈청은 각각 BRV 및 BCV 항원과만 특이적인 결합반응을 보였다. 면역에 따른 혈청항체 및 난황항체의 수준은 실험 8~12주차에 최고도에 달했고, BRV에 대한 항체의 경우 혈청과 난황내에서 면역 후 12주째에 각각 약 104,000과 107,000의 역가 수준을 보였으며, BCV의 경우 8주째에 각각 약 145,000과 155,000의 수준을 보였다. BRV 및 BCV에 대한 중화능력 유무 확인을 위하여 분리된 난황항체를 이용하여 혈구응집억제반응 시험을 실시한 결과, BRV 및 BCV에 대한 혈구응집억제 희석비가 각각 5,120 및 1,260으로 면역하지 않은 대조군에 비하여 8배 이상 높은 중화력을 나타냈다. 이러한 결과를 종합하면, 산란계에 BRV 및 BCV를 면역하여 얻어진 난황항체는 BRV 및 BCV에 대한 면역 특이성을 가지고 중화할 수 있는 능력이 있으며, 이러한 난황항체는 BRV 및 BCV의 증식을 효과적으로 억제시킬 수 있어 임상에 적용할 경우 유용할 것으로 사료된다.

• 한국식품조리과학회지
• /
• 제17권6호
• /
• pp.589-597
• /
• 2001

This study was conducted to develop a standardized Korean cold noodle stock recipe which can be used in food service establishments. The qualities of three kinds of stock made of beef rib only(B group), beef rib added with chicken(BC group), and beef rib added with chicken and vegetable(BCV group) were investigated by using sensory evaluation and instrumental analyses for free amino acids and nucleotides during heating (2, 3, 4, and 6 hr) at 90$\pm$ 5$\^{C}$ The highest amino acid contained in B, BCV, and BCV groups was arginine followed by alanine, glycine, and glutamic acid. B and BC groups was extracted to the best contents of amino acids by heating for 4 hours but BCV group for 3 hours. Nucleotides were extracted from B and BC group between 3 and 4 hours of heating but in BCV group between 2 and 3 hours. In sensory evaluation, BCV group obtained the highest score for overall preference. In the measurement of color difference, BCV group was the lowest in L value(lightness) but the highest in b value(yellowness).

• 한국동물위생학회지
• /
• 제22권3호
• /
• pp.239-245
• /
• 1999

The reverse transcriptase polymerase chain reaction (RT-PCR) was used for the detection of bovine coronavirus (BCV) in fecal samples by using reverse transcriptase and two primers which flanked M gene sequence of 407bp. RT-PCR detected bovine coronavirus specifically, but did not detect mouse hepatitis virus (MHV), transmissible gastroenteritis virus (TGEV), and bovine rotavirus (BRV). The M gene sequences of MHV are homologus to that of BCV, but minor differences exist in the primer regions, preventing annealing of the primers. Detection of BCV using RT-PCR was compared with ELISA and the agreement of BCV detection by RT-PCR and ELISA was 95.3%. RNA detection in positive clinical specimens was significantly better by PCR than immunological detection of BCV by ELISA.

• 한국동물위생학회지
• /
• 제32권3호
• /
• pp.209-213
• /
• 2009

This survey was carried out to investigate the prevalence of bovine coronavirus (BCV) infection for the calves in Seosan-Taean Area. A total of 75 samples were collected from fecal swab to detect BCV by RT-PCR Results obtained through the survey were as follows; By RT-PCR(455bp) BCV was detected from 13 of the 75 sample of fecal swab from calves. The calves under 3 month showed the highest BCV detection rate.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2008년도 춘계학술대회논문집
• /
• pp.613-617
• /
• 2008

One of the important parts on multimedia era is acoustic ones. With increased demand of smallest multimedia products such as personal digital assistant (PDA) and mobile phones, it is necessary to develop acoustic devices which have higher performance and smaller size. Acoustic parts with various function for hearing impaired persons. This paper introduces a bone conduction vibrator (BCV) for hearing impaired persons to use portable acoustic device without additional devices. For vibration analysis of the BCV, electromagnetic, mechanical and their coupling effects are considered for the analysis. This paper shows that the development of design and analysis technique by finite element method (FEM) of BCV.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
• /
• pp.599-601
• /
• 2004

A new blue electroluminescent conjugated polymer, poly(BP-alt-BCV), was prepared by Hornor-Emmons polycondensation and used as a host polymer for the phosphorescent dopant, $(bsn)_2Ir(acac)$. Poly(N-vinylcarbazole) (PVK), known as a blue EL material, was also used for comparison with poly(BP-alt-BCV). Electrophosphorescence of PLEDs with these dopant/host systems was investigated in terms of luminescence, efficiency, emission color, and energy transfer.

• 한국동물위생학회지
• /
• 제21권2호
• /
• pp.127-132
• /
• 1998

To study the efficacy monoclonal antibody(MAb) against bovine rotavirus(BCV) in treatment of calf diarrhea, the MAb was fed to 166 calves with diarrhea from Chung-buk area. The results were summarized as follows. 1. Among the 1,049 calves investigated, 166(16%) calves were infected with BCV. 2. The monthly rate of BCV infection were higher in October to December compared with other months of the year. 3. Among the 166 calves with diarrhea, 137(83%) calves were recovered. 4. Young calves within 7-day-old were more effective in treatment than other ages and the rate of treatment was 86% 5. Most effective period for treatment of rotavirus was at the first stage of infection.

• 대한수의학회지
• /
• 제38권3호
• /
• pp.581-588
• /
• 1998

Eight monoclonal antibodies(MAbs) against bovine coronavirus(BCV) were produced and characterized. Three MAbs(1G9, 4H12, 5C1) specific to the S glycoprotein and two HE glycoprotein-specific MAbs(2A5, 5G4) were found to neutralize the BCV in fluorescence focus neutralization(FFN) test. Two HE-specific MAbs from the neutralizing MAbs inhibited the hemagglutinating activity of the BCV. None of the N protein-specific MAbs(1C1, 5A12, 6H1) neutralized the virus infectivity. Bovine coronavirus and mouse hepatitis virus, which belong to group II coronaviruses, were differentiated from other groups of coronaviruses(porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus, canine coronavirus) by all MAbs in fluorescence antibody test(FA), but not in FFN test.

• 현장농수산연구지
• /
• 제5권1호
• /
• pp.57-63
• /
• 2003

The bovine rotavirus(BRV), bovine coronavirus(BCV) and bovine viral diarrhea virus(BVDV) are main viruses of bovine viral diarrhea disease. These viruses could be rapidly amplified by the reverse transcriptase polymerase chain reaction(RT-PCR). This study was conducted to develop rapid and accurate diagnostic methods of these viral diseases by multiplex RT-PCR. Specific primers were designed based on the sequences reported by Chang KO et. al. (1997) and Schroeder BA, et. al. (1990), RNA were prepared from the cultured viruses, first-stranded DNAs were synthesised by reverse transcriptase. PCR were conducted to amplify specific regions of the viruses by multiplex. Three bands such as 1,062bp for BRV, 458bp for BCV, and 300bp for BVDV were successfully produced by multiplex RT-PCR. In conclusion, this result suggested that these viruses could be diagnosed rapidly and accurately by multiplex RT-PCR.

• Journal of applied mathematics & informatics
• /
• 제3권2호
• /
• pp.205-216
• /
• 1996

The field of nonparametrics has shown its appeal in re-cent years with anarray of new tools for statistical analysis. As one of those tools nonparametric regression has become a prominent statis-tical research topic and also has been well established as a useful tool. In this article we investigate the biased cross-validation selector, BCV, which is proposed by Oh et al. (1995) for a less smoothing regression function. In the simulation study BCV selector is shown to perform well in parctice with respect to ASE ratio.