• Radiation Oncology Journal
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• v.14 no.4
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• pp.265-279
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• 1996

Damage produced by radiation elicits a complex response in mammalian cells, including growth rate changes and the induction of a variety of genes associated with growth control and apoptosis. At doses of 10,000 cGy or greater, the exposed individual was killed in a matter of minutes to a couple of days, with symptoms consistent with pathology of the central nervous system(CNS) including degenerative changes. The nature of the damage in irradiated cells underlies the unique hazards of ionizing radiation. Radiation injury to CNS is a rare event in clinical medicine, but it is catastrophic for the patient in whom it occurs. The incidence of cerebral necrosis has been reported as high as 16% for doses greater than 6,000 cGy. In this study, the effect of radiation on brain tissue was studied in vivo. Jun and p53 genes in the rat brain were induced by whole body irradiation of rat with 600Co in doses between 1 Gy and 100 Gy and analyzed for expression of jun and p53 genes at the postirradiation time up to 6 hours. Northern analyses were done using 1.8 Kb & 0.8 Kb-pGEM-2-JUN/Eco RI/Pst I fragments, 2.0 Kb-php53B/Bam HI fragment and ,1.1 Kb-pBluescript SK--ACTIN/Eco RI fragment as the digoxigenin or [${\alpha}^{32}P$] dCTPlabeled probes for Jun, p53 and ${\beta}$-actin genes, respectively. Jun gene seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 1 hour after irradiation of $^{60}$Co in dose of 30 Gy. Jun was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in doses of 1 Gy and 10 Gy. After irradiation of $^{60}$Co in dose between 20 Gr and 100 Gy, the expression of Jun was however increased to peak in 2 hours and decreased thereafter. p53 gene in this study also seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 6 hours after irradiation of $^{60}$Co in dose of 1 Gy, p53 was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in dose between 1 Gy and 40 Gy. After irradiation of $^{60}$Co in doses of 50 Gy and 100 Gy, the expression of p53 was however increased to peak in 2 hours and decreased thereafter. The expression of Jun and p53 genes was not correlative in the brain tissue from rats. It seemed to be very important for the establishment of the optimum conditions for the animal studies relevant to the responses of genes inducible on DNA damage to ionizing radiation in mammalian cells. But there are many limitations to the animal studies such as the ununiform patterns of gene expression from the tissue because of its complex compositions. It is necessary to overcome the limitations for development of in situ Northern analysis.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.153-160
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• 2006

Brassica rape is an important species used as a vegetable, oil, and fodder worldwide. It is related phylogenically to Arabidopsis thaliana, which has already been fully sequenced as a model plant. The 'Multinational Brassica Genome Project (MBGP)'was launched by the international Brassica community with the aim of sequencing the whole genome of B. rapa in 2003 on account of its value and the fact that it has the smallest genome among the diploid Brassica. The genome study was carried out not only to know the structure of genome but also to understand the function and the evolution of the genes comprehensively. There are two mapping populations, over 1,000 molecular markers and a genetic map, 2 BAC libraries, physical map, a 22 cDHA libraries as suitable genomic materials for examining the genome of B. rapa ssp. pekinensis Chinese cabbage. As the first step for whole genome analysis, 220,000 BAC-end sequences of the KBrH and KBrB BAC library are achieved by cooperation of six countries. The results of BAC-end sequence analysis will provide a clue in understanding the structure of the genome of Brassica rapa by analyzing the gene sequence, annotation and abundant repetitive DHA. The second stage involves sequencing of the genetically mapped seed BACs and identifying the overlapping BACs for complete genome sequencing. Currently, the second stage is comprises of process genetic anchoring using communal populations and maps to identify more than 1,000 seed BACs based on a BAC-to-BAC strategy. For the initial sequencing, 629 seed BACs corresponding to the minimum tiling path onto Arabidopsis genome were selected and fully sequenced. These BACs are now anchoring to the genetic map using the development of SSR markers. This information will be useful for identifying near BAC clones with the seed BAC on a genome map. From the BAC sequences, it is revealed that the Brassica rapa genome has extensive triplication of the DNA segment coupled with variable gene losses and rearrangements within the segments. This article introduces the current status and prospective of Korea Brassica Genome Project and the bioinformatics tools possessed in each national team. In the near future, data of the genome will contribute to improving Brassicas for their economic use as well as in understanding the evolutional process.

• Journal of Sasang Constitutional Medicine
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• v.13 no.2
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• pp.28-48
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• 2001

1. Background and Purpose Since ${\ulcorner}$Dongri Sasang Shinpyun${\lrcorner}$ was published in 1929, it had great effects on the publications related to Sasang Constitutional Medicine. However there had been no practical research or its applications about ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ at all in spite of its importance. So through the study on the ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ we highly intend to utilize the book. 2. Methods In this dissertation, you'll find our research of ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ regarding the author, the people who were involved in the publishing, the formations and the contents, our another research of ${\ulcorner}$Dongyi Sasang Shinpyun Chebang${\lrcorner}$(東醫四象新編劑方) to make its medical characteristics and meaning clear. 3. Results and Conclusion 1) Won Chi Sang was Chang Bong Young's granduncle's son-in-law. Nam Dae-hee is suspected of Nam Tae-hee who lived in YeoJu Bam Gol at that time though, it's not certain. 2) ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ is consisted of two pans. ${\ulcorner}$The Internal part${\lrcorner}$ is the basic explanation and ${\ulcorner}$The External part${\lrcorner}$, is the prescriptions depending on the symptoms. ${\ulcorner}$Sasang Byunron${\lrcorner}$,(四象辯論) is mostly that's for the explanations about analysis of Sasang Constitution. ${\ulcorner}$Sasang Kyunghum${\lrcorner}$(四象經驗) is mainly it's for the prescriptions depends on Sasangin's symptoms. ${\ulcorner}$Sasang Kukyul${\lrcorner}$(四象口訣), ${\ulcorner}$Tong Sasang Changbu Sochaedo${\lrcorner}$(通四象臟腑所在圖), ${\ulcorner}$Tong Sasang Chunche Sosokdo${\lrcorner}$(通四象全體所屬圖) are about the analysis of Sasangin's characteristics from metaphysical concepts to Changbustic concepts. In ${\ulcorner}$Sasangin oyak${\lrcorner}$(四象人要藥), among them, 10 types of medicines were categorized separately by constitutional difference in ${\ulcorner}$Dongui Sasang Shinpyun${\lrcorner}$ and ${\ulcorner}$Dongmu Youg${\lrcorner}$(東武遺槁). The unique style of ${\ulcorner}$Dongyi Sasang Yongyak Huebun${\lrcorner}$(東醫四象用藥索分) is originated from ${\ulcorner}$Bangyak Happyun${\lrcorner}$ ${\ulcorner}$Euibang Whaltu${\lrcorner}$(${\ulcorner}$方藥合編${\lrcorner}$${\ulcorner}$醫方活套${\lrcorner}$), ${\ulcorner}$Whaltu Chimsun${\lrcorner}$,(${\ulcorner}$方藥合編${\lrcorner}$ ${\ulcorner}$活套鎭線${\lrcorner}$). 3) There are 293 prescriptions in ${\ulcorner}$Dongyi Sasang Shinpyun Chebang${\lrcorner}$. 36 out of 44 prescriptions in ${\ulcorner}$Gapont${\lrcorner}$(甲午本) are quoted in ${\ulcorner}$kyunghumbang${\lrcorner}$. Therefore it could be very possible that those unknown prescriptions in ${\ulcorner}$Kyunghumbang${\lrcorner}$ can be from lee Je Ma. 4) We are assumed that ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ was made by some other doctors not from one single person, based on Lee Je Ma's prescriptions, after Lee Je Ma died. ${\ulcorner}$Dongyi Sasang Shinpyun${\lrcorner}$ is very different from ${\ulcorner}$Dongeyi Suse Bowon${\lrcorner}$, ${\ulcorner}$Dongmu Yougo${\lrcorner}$(東武遺稿). ${\ulcorner}$Chobonkwun${\lrcorner}$(草本卷) by lee Je Ma since it was published for utilizing Sasang Constitutional Medicine and medical practice not for the basic principles of Sasang Constitution by Lee Je Ma. Therefore it could be highly possible to look over the spirit of Sasang Constitution by lee Je-ma.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.922-929
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• 2019

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.