• Bulletin of the Korean Chemical Society
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• 제25권12호
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• pp.1845-1849
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• 2004

Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 ${\AA}^2$ and the total RMSD was 0.5-1.5 ${\AA}. Moreover, pB1 structure included a weak $3_{10}$-helix from $Ile^7$,/TEX> to $Trp^{13}$.

• 한국직업건강간호학회지
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• 제12권2호
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• pp.156-163
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• 2003

The 5 year follow up study was conducted to assess the positive seroprevalence of HbsAg and magnitude of new cases in a steel manufacturing big scale workpalce. About 2,000 serum samples collected from 1998 to 2002 for hepatititis B surface antigen(HBsAg), antibody to HBsAg(anti-HBs), sAST, sALT, r-GTP, total cholesterol, and triglyceride were tested. The commercially available enzyme-linked immunosorbent assay was utilized in serologic test of hepatititis B surface antigen (HBsAg), and antibody to HBsAg(anti-HBs). The preceived seroprevalence rates in the study were ranged 6.3~6.9%. The seroprevalences of HbsAg were 4.3~4.9% among the age of thirties, a significantly decreased seroprevalence compared with those among other age groups(in forties, 7.1~8.2%, and in fifties 7.1~7.6%). The positive seroprevalence of anti-HBs were 71.0~77.9%. A new case was not detected in the group.

• 대한수의학회지
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• 제39권6호
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• pp.1151-1160
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• 1999

This study was conducted to detect the toxoplasma-specific DNA in peripheral blood collected from cats experimentally infected with Toxoplasma gondii (RH strain) and from domiciled cats by B1 gene-base polymerise chain reaction(PCR). The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with parasite detection by mouse inoculation(MI). And also, latex agglutination test(LAT) and indirect fluorescent antibody test(IFAT) were conducted to detect the fluctuation of serum antibodies compared with the detection of toxoplasma by PCR and MI. Toxoplasma B1 gene PCR was shown consistently high sensitivity and the results obtained by PCR agreed completely with those from MI. All blood samples collected before infection with T gondii gave negative results by PCR and MI. Also, toxoplasma Bl gene PCR was not cross reaction with Neospora caninum DNA and normal cat leucocyte as controls. The toxoplasma-specific DNA was detected by PCR in blood of 5 cats experimentally infected with T gondii 6 days after infection and the detection of this specific-DNA was long lasted in blood for 64 days after infection. The detection of toxoplasma-specific DNA by PCR could be identified as few as 10 tachyzoites and the isolation of T gondii by MI could be isolated as few as 1 tachyzoite from tenfold serial dilution of T gondii with normal cat blood, respectively. In healthy domiciled cats, the toxoplasma-specific DNA and the parasite were detected and isolated in blood from 3 of 56(5.3%) cats by both PCR and MI, respectively. In the results of antibody test from the total 56 heads of healthy domiciled cats, the positive rates are 15(26.7%) by LAT and 19(33.9%) by IFAT. These results suggest that PCR detection of toxoplasma can be applied as a sensitive and specific diagnostic and research tool.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1336-1344
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• 2017

Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma. With recent identification of HBV receptor, inhibition of virus entry has become a promising concept in the development of new antiviral drugs. To date, 10 HBV genotypes (A-J) have been defined. We previously generated two murine anti-preS1 monoclonal antibodies (mAbs), KR359 and KR127, that recognize amino acids (aa) 19-26 and 37-45, respectively, in the receptor binding site (aa 13-58, genotype C). Each mAb exhibited virus neutralizing activity in vitro, and a humanized version of KR127 effectively neutralized HBV infection in chimpanzees. In the present study, we constructed a humanized version (HzKR359-1) of KR359 whose antigen binding activity is 4.4-fold higher than that of KR359, as assessed by competitive ELISA, and produced recombinant preS1 antigens (aa 1-60) of different genotypes to investigate the binding capacities of HzKR359-1 and a humanized version (HzKR127-3.2) of KR127 to the 10 HBV genotypes. The results indicate that HzKR359-1 can bind to five genotypes (A, B, C, H, and J), and HzKR127-3.2 can also bind to five genotypes (A, C, D, G, and I). The combination of these two antibodies can bind to eight genotypes (A-D, G-J), and to genotype C additively. Considering that genotypes A-D are common, whereas genotypes E and F are occasionally represented in small patient population, the combination of these two antibodies might block the entry of most virus genotypes and thus broadly neutralize HBV infection.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제27권2호
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• pp.104-112
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• 2024

Purpose: Immunosuppressive therapy is frequently administered to patients with inflammatory bowel disease (IBD), which may make them more susceptible to infections like hepatitis B. Methods: A cross-sectional study was conducted on patients aged 5-18 years diagnosed with IBD who visited a gastroenterology clinic along with controls who were the same age as the patients with IBD and were healthy overall. A logistic regression analysis using the independent variables of age, sex, race, disease phenotype, surgery, and medications and the dependent variable of adequate hepatitis B surface antibody (HBsAb) titers (>10 mIU/mL) was performed on quantitative serum HBsAb titers. Results: The study enrolled 62 patients, including 37 males and 25 females. Crohn's disease, ulcerative colitis, and indeterminate colitis were diagnosed in 16, 22, and 24 patients, respectively. Thirty-nine patients were taking corticosteroids at the time of the study, 42 were taking immunomodulators, and four were taking biologics. Compared to 44.7% of the control group, 9.3% of the patients had protective titers. Only 12 out of 62 patients had HBsAb titers greater than 10 million IU/mL. None of the patients who received biologics or corticosteroids and 3.2% of those who received immunomodulators were found to be seroimmuned. Conclusion: The younger patients had the highest titers. Patient-specific factors that may impact these low titers include the length of the patient's illness and the use of immunosuppressants.

• 한국동물위생학회지
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• 제24권2호
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• pp.147-159
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• 2001

This Study was conducted to determine vaccination programs for the control of Newcastle Disease(ND) in chickens and investigate protective effect against Newcastle disease virus (NDV) after live ND vaccination. Maternal HI antibody titer level of chickens according to day(age) 1, 7, 14, 21, 28 and 35 were decreased gradually as 7.10$\pm$0.74, 6.57$\pm$0.74, 3.71$\pm$1.25, 2.20$\pm$1.03, 1.20$\pm$1.23 and 0.50$\pm$0.71. As a result of HI test and ELISA, both chickens vaccinated with VG/GA strain live vaccine at 1-day-old and chickens not vaccinated do not have antibody titer for protection against NDV at 14-day-old. Except for LaSota strain vaccine, in case of vaccination with VG/GA spray and VG/GA, B1 and LaSota strain drinking water at 14-day-old, the protective effect was 100% in chickens inoculated NDV($10^{7.2}$ $EID_{50}$/50${\mu}\ell$, eye drop) at 21-day-old, but not 10～50% at 28-day-old. These data suggest that live NDV vaccination should be given at 10-day-old 20-25day-old for protect against NDV at periodic outbreaks of ND caused by velogenic viscerotropic NDV in the environment of a farm.

• Journal of Preventive Medicine and Public Health
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• 제27권4호
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• pp.747-761
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• 1994

Workers' periodic health examination is the main tools used to manage the health problems of most workers in Korea. The most common health problem found in workers' periodic health examination is liver disorder. Liver disorder is also one of the most common health problems in general population and one of the leading causes of mortality in adult population. Regulation proposed by government (No. 207, Ministry of Labor, 1992) defines the criteria for selection of workers with the liver dysfunction for further evaluative examination and the examination items used for diagnosis of the workers with liver dysfunction. This study was designed to evaluate the proficiency of each examination items presently defined in Regulation and propose the optimal examination items for detection of the liver disorders found by workers' periodic health examination. Study subjects are 186 workers with abnormal liver function tests in screening examination of workers' periodic health examination. Questionnaire survey including past history of liver disorder, drinking history, height and weight was done. Physical examination by physician, routine test items defined by Regulation (SGOT, SGPT, $\gamma$-GTP, protein, albumin, total and direct bilirubin, alkaline phosphatase, $\alpha$-feto protein, HBsAg and anti-HBs), anti-HCV antibody test and liver ultrasonography were done. Results are as follows; 1. Result of evaluative examination utilizing only the items defined in Regulation was; There were 75 workers with suspected live. disorder(40.3%), 63 with no liver dysfunction (33.9%), 13 with suspected hepatitis B(7.0%), 10 workers with hepatitis B(5.4%), 10 workers with hepatitis B carrier state(5.4%), 10 with alcoholic liver disorders(5.4%), 5 with fatty liver(2.7%). When alternative diagnostic criteria applying additional examination items (drinking history, body mass index, anti-HCV antibody and ultrasonography) diagnosability of liver disorder was increased. When all four items were included, final results were; 23 workers (17.8%) with hepatitis B (10 carriers, 13 suspects and 10 hepatitis B), 10 (5.4%) with hepatitis C(4 carriers, 5 suspects and 1 hepatitis C), 13(7.0%) with alcoholic liver disorder, 45(24.2%) with fatty liver (40 suspects, 5 fatty liver), 410%) with suspected liver disorders and 44 (23.7%) with normal liver. 2. Of examination items defined by Regulation, only SGOT, SGPT, $\gamma$-GTP and HBsAg were significantly different in abnormal rate and mean value, and all other laboratory findings did not showed significant difference between two groups. Drinking history, body mass index and anti-HCV antibody test which are the items that authors included in this study, also showed significant difference between two groups. Utilization of body mass index (BMI) for abnormal liver function group in diagnosis of fatty liver had high specificity (97.6%) but sensitivity (22.3%) was low. Therefore we suggest that SGOT, SGPT, $\gamma$-GTP, HBsAg, alcohol drinking history, BMI and anti-HCV Ab were useful for diagnosis of liver disorders among worker's periodic health examination.

• 핵의학기술
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• 제12권1호
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• pp.78-81
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• 2008

목적: 본 연구의 목적은 치료제를 복용하는 만성 B형간염환자의 혈청에서 seroconversion되는 과정 중에 불안정하게 나타나는 HBeAg과 HBeAb 사례를 연구하여, 검사자가 검사결과에 대한 정확한 이해를 하는데 도움을 주고자 하는 것이다. 방법: 만성 B형간염 환자로 진단을 받고 치료제를 복용하고 HBeAg 검사와 HBeAb검사를 시행한 3명을 대상으로 하였다. HBeAg과 HBeAb검사는 Radioimmunoassay법과 Immunoradiometric assay법으로 실시되었다. 결과: HBeAg이 음성화되는 과정에서 결과가 양성에서 음성으로, 다시 양성으로 불안정한 결과를 보였다. HBeAb가 양성화되는 과정에서 결과가 음성에서 양성으로, 다시 음성으로 변하는 불안정한 결과를 보였다. 결론: 이번 사례는 seroconversion 유도를 위해 약물치료중인 만성 B형간염 환자에서 볼 수 있는 불안정한 HBeAg과 HBeAb 혈액 결과이다. 불안정한 HBeAg과 HBeAb 검사 결과를 통하여 검사자는 환자가 바이러스 치료제를 복용하여 seroconversion되는 과정 중에 있을 수 있다는 것과 불안정한 결과는 치료제에 의해 일어나는 자연스러운 현상임을 알 수 있다.

• 대한핵의학회지
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• 제37권6호
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• pp.402-417
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• 2003

목적: 종양의 진단과 치료에 널리 이용되고 있는 단클론항체를 수용체에 결합하는 수송체로 이용할 수 있는지에 대한 가능성 여부를 평가하기 위하여, 간의 asialoglycoprotein 수용체에 결합할 수 있는 갈락토즈접합 단클론항체를 $^{111}In$로 표지하여 체내에서의 분포와 간을 중심으로 한 체내대사를 분석하였고, 그 결과를 갈락토즈를 접합하지 않은 $^{111}In$ 표지 항체와 비교하였다. 재료 및 방법: 인체 림프 구성백혈병 세포에 대한 T101 단일클론항체를 cyclic DTPA dianhydrate(DTPA) 나 2-p-isothiocy-anatobenzyl-6-methyl-DTPA(IB4M) 로 접합하고 갈락토즈를 붙인후 $^{111}In$으로 표지하였다. 생쥐와 흰쥐에서 갈락토즈를 접합한 화합물과 접합하지 않은 화합물의 체내분포와 간대사를 비교분석하였다. 결과: $^{111}In$ 표지 T101항체와 갈락토즈 접합체는 투여량의 대부분이 10분 이내에 간에 섭취되었다. DTPA 접합자를 사용한 경우 IB4M 접합자를 사용한 경우보다 간에 오랫동안 저류되어 주사 후 44시간 간 섭취율이 각각 55%와 20% 였다. 이 기간동안의 DTPA화합물의 방사성 대사산물은 24%가 소변으로 17%가 대변으로 배설되어 유사하였으나 IB4M 화합물은 68%가 대변으로 8%가 소변으로 배설되어 배설경로에 차이가 있었다. 1B4M화합물을 주사후 3시간의 담즙과 간 현탁액을 HPLC로 분석한 결과 IgG와 저류시간(Rt)이 같은 첫 절정에 35%,유리 $^{111}In$과 유사한 절정의 Rt에 65%가 관찰되어 대사산물이 빠르게 답즙으로 배출됨을 알 수 있었고, DTPA 화합물 주사후 3시간 대사산물은 90%가 $^{111}In-DTPA$와 유사한 Rt의 절정을 보였다. 그러나 대변의 $^{111}In$ 의 축적량은 낮아 DTPA 접합화합물은 담도를 통한 빠른 배설이 일어나지 않음을 알 수 있었다. 결론: 단일클론항체에 갈락토즈를 접합한 경우보통의 항체에 비하여 간 섭취가 많고, 간에서의 대사가 촉진된다. 이 경우 사용되는 접합자의 선택에 따라서 대사산물의 성분이 달라지고 간에서의 제거도 차이가 있다. 이러한 대사의 차이점은 향후 종양세포나 조직의 탐색에 이용할 방사능 표지 항체의 제조에 응용될 수 있을 것이다.

• 대한의생명과학회지
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• 제23권3호
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• pp.175-184
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• 2017

Cholera toxin (CT) is an ADP-ribosylating bacterial exotoxin that has been used as an adjuvant in animal studies of oral immunization. The mechanisms of mucosal immunogenicity and adjuvanticity of CT remain to be established. In this study, we investigated the role of indoleamine 2,3-dioxygenase (IDO), which participates in the induction of immune tolerance, in CT-mediated breakdown of oral tolerance. When IDO-deficient ($IDO^{-/-}$) mice and their littermates were given oral ovalbumin, significant changes in antibody responses, footpad swelling and $CD4^+$ T cell proliferation were not observed in $IDO^{-/-}$ mice. Feeding of CT decreased IDO expression in mesenteric lymph nodes (MLN) and Peyer's patch (PP). CT-induced downregulation of IDO expression was reversed by inhibitors of nuclear factor-kappa B (NF-${\kappa}B$), pyrrolidine dithiocarbamate and p50 small interfering RNA. IDO expression was downregulated by the NF-${\kappa}B$ inducers lipopolysaccharide and tumor necrosis factor-${\alpha}$. CT dampened IDO activity and mRNA expression in dendritic cells from MLN and PP. These data indicate that CT disrupts oral tolerance by activating NF-${\kappa}B$, which in turn downregulates IDO expression. This study betters the understanding of the molecular mechanism underlying CT-mediated abrogation of oral tolerance.