• Title/Summary/Keyword: B13

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Analysis of Genotype and Phenotype of Erythromycin Resistance in Enterococci spp. Isolated from Raw Milk Samples (원유시료에서 분리한 장구균의 에리스로마이신 내성 유전자형 및 표현형 분석)

  • Lee, Hye-In;Jung, Jae-Hyuk;Lee, Sang-Jin;Choi, Sung-Sook
    • Korean Journal of Microbiology
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    • v.46 no.2
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    • pp.148-151
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    • 2010
  • The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ phenotype. All of isolates have erm(B) determinant, 75.45% (n=83) have mef(A) an efflux system determinant. The majority of Enetrcococci isolated from raw milk samples in northern area of Gyeonggi-Do showed high level of resistance to erythromycin.

A study on Actinosporeans parasitized in Branchiura sowerbyi (Oligochaeta : Annelida) (아가미지렁이 (Branchiura sowerbyi)에 기생하는 포자충류에 관한 연구)

  • Kim, Young-Gill
    • Journal of fish pathology
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    • v.8 no.1
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    • pp.13-21
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    • 1995
  • Four species of Actinosporeans, Aurantiactinomyxon sp. A, B, C and Neoctinomyxon sp. D were released from Oligochaete, Branchiura sowerbyi captured from three reserviors, where intestinal giant-cystic disease in carp had occured from June to September, 1994. All part of the intestinal epithelial tissue from the gullet to the anus of B. sowerbyi were infected by Actino-sporeans, and many mature Actinosporean were seen more easily at the posterior parts of the body. Just before releasing, mature Actinosporean sporozoites were divied into each individual from the intestinal epithelial tissue of Oligochaete, while immature ones had 6 spores ($20{\times}25{\mu}m$ in size) per each in the oocyst ($60{\times}65{\mu}m$ in size). A total of 1, 762 of B. sowerbyi were investigated in three reservoirs, 86 individuals (4.88%) of them were infected; 0. 74% (13 ind.) of Aurantiactinomyxon sp. A, 2. 27% (40 ind.) of Aurantiactinomyxon sp. B, 1. 59% (28 ind.) of Aurantiactinomyxon sp. C, and 0. 28% (5 ind.) of Neoactinomyxon sp. D. At the room temperature of 22.6-$30.7^{\circ}C$, number of extrusion dates of Actinosporeans from B. sowerbyi for 32 days are 1 day (23.3% of total, 1 time) or 5 days (11.7%. 5 times), and the majority was finished within 15 days, however, 6.7% of total were released for 32 days.

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Spraying and Combustion Characteristics of Heavy Oil in the Gun Type Burner for Hot Air Heater (온풍난방기용 건타입 중유버너의 분사특성과 연소특성)

  • 김영중;유영선;장진택;윤진하;연태용
    • Journal of Biosystems Engineering
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    • v.24 no.2
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    • pp.107-114
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    • 1999
  • To find the best combustion conditions in the heavy oil burner kinetic viscosity of heavy oil A, B and C at different temperature range, from 40 to 140$^{\circ}C$, and the droplet sizes of the heavy oils at different temperature and pump pressure were measured. And, combustion characteristics were investigated under the different conditions : two different heavy oil and three different oil temperature. At temperature of 70, 100, 130$^{\circ}C$ the kinetic viscosity of heavy oil A and B are 7.9, 5.7, 4.3 and 30.4, 13.7, 7.9cSt, respectively. The greatest and smallest viscosity were 7,455 cSt at C oil on 27$^{\circ}C$ and 4.26cSt at A oil on 140$^{\circ}C$. The magnitude of viscosity difference between at 100$^{\circ}C$ and 140$^{\circ}C$ under 6 cSt in cases of A and B oil, but more than 30cST on C oil. Of the droplet sizes, the biggest and smallest droplet size in A oil were 98$\mu\textrm{m}$ at oil temperature of 130$^{\circ}C$(4.3cSt), pump pressure of 1.57MPa and 72$\mu\textrm{m}$ at 70$^{\circ}C$(7.9cSt), 2.35MPa, respectively. It appeared that as spraying pressure increased the droplet size decreased, however, no distinct differences were found in the effects of kinetic viscosity on the droplet sizes of the test range. The best combustion performance was observed when droplet size, spraying pressure and oil temperature were 73$\mu\textrm{m}$, 2.35MPa and 70$^{\circ}C$ producing CO2 of 13.1%, CO of 13ppm and flue gas temperature of 250$^{\circ}C$ in A oil combustion For B oil, it was100$^{\circ}C$, 2.35MPa, 52$\mu\textrm{m}$, producing CO2 of 10ppm and flue gas temperature of 260$^{\circ}C$. In general, it appeared that better combustion results were observed in the smaller droplets produced burner condition.

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Effect of Nutrition Education Pogram on Obese Middle Shoot Boys in Taegu (대구 지역의 일부 비만중학생을 위한 영양교육 프로그램의 효과)

  • Choi, Bong-Soon;Lee, In-Sook;Shin, Han-Sul
    • Journal of the Korean Society of Food Culture
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    • v.13 no.4
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    • pp.383-391
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    • 1998
  • This study was conducted to find the effect of in-class nutrition education and exercise program for obese adolescent boys in Taegu with the nutrition knowledge test before and after education program and anthropometric measurement. The subjects were consisted of a normal group (NG; n= 13) and an obese group (n=30). The obese group is divided into two groups; obese group A (OG-A; n=12), participated in a ten-week nutrition education only; and obese group B (OG-B; n=28), participated in a ten-week nutrition education and exercise program. The anthropometric data of the three groups were increased after the nutrition education program. However the obesity index(BMI, R hrer, WHR, Skinfold thickness) of OG-B were significantly decreased after the programs. The nutrition intake data were also changed after the nutrition education program. Especially the intake of carbohydrate was significantly decreased in OG-B from $421.5{\pm}155.9g$ to $349.0{\pm}41.0g$ (p<0.05) after the nutrition education program. It was noted that the intake of Ca was significantly decreased in all three groups after the nutrition education program. The mean score of the nutrition knowledge test also significantly increased from $13.7{\pm}3.1$ to $17.8{\pm}3.0$ after the nutrition education program. The result strongly suggested that nutrition education program should be successful if the obese adolescents and their parents especially mothers participated in the same class. In summary, nutrition education and exercise programs were effective on reducing obesity of obese adolescents. Nutrition knowledge of obese adolescent was significantly increased after nutrition education program.

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Reflection Characteristics of Vortical Slit Caisson Breakwater (종 SLIT형 케이슨 방파제의 반사특성)

  • 이종인;조지훈
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.13 no.4
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    • pp.263-272
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    • 2001
  • Recently, some attempts to construct slit caisson-type breakwaters are made in Korea. Since slit caisson-type breakwaters are suitable for relatively deep sea areas, a lot of theoretical and experimental researches have been performed. In this study, the reflection characteristics of vertical slit caisson breakwaters are investigated based on the measured data in two-dimensional hydraulic model tests with irregular waves. The experiments were conducted for various cases; variation of porosity of perforated-wall, width of wave chamber, number of slits for single-and double-chamber, respectively. It is found that in the case when the wave steepness (H/L$_{s}$ ) is small, the reflection coefficients are large. The existing researches have shown that the wave reflection is minimized when the nondimensional width of wave chamber B/L is about 0.2~0.25 for the regular waves. However, for the irregular waves the reflection is lowest when $B/L_2$, is 0.13~0.15. For a same porosity condition, the wave dissipation is stronger as the width of s1it is larger. The double-chamber caisson is superior to single- chamber caisson in the wave dissipating effects.

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Effects on Mechanical Properties, Joint Range of Motion, and Grip Strength of Forearm Muscles Depending on Wrapping Direction of the Floss Band

  • Ki Bum Jung;Seohui Kim;Yongwoo Lee
    • Physical Therapy Rehabilitation Science
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    • v.13 no.1
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    • pp.95-105
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    • 2024
  • Objective: The purpose was to investigate changes in mechanical properties, range of motion, and grip strength of the forearm muscle in 13 students depending on the wrapping direction of the floss band. Design: A single-blind, crossover study Methods: Subjects were randomly assigned to the wrapping direction of the flossing band and then performed a concentric exercise of the wrist flexors using a Flex-Bar. Intervention A applied the flossing band in a right spiral direction, Intervention B applied the flossing band in a left spiral direction, and Intervention C performed the exercise alone. All subjects used their dominant right hand, and pre- and post-assessments were conducted between interventions. To analyze differences in changes between groups pre- and post-, all results were subjected to one-way ANOVA, followed by Scheffe's test as a post-hoc analysis. The paired samples t-test was used to analyze the difference between pre- and post-change within groups. Results: First, in the mechanical properties of the Flexor carpi ulnaris (FCU) muscle, interventions A and B significantly improved muscle tone and stiffness than intervention C (p<0.05), and intervention A showed a significant decrease in decrement (muscle elasticity) than intervention B (p<0.05). Second, interventions A and B showed significant improvement in grip strength than intervention C (p<0.05). Conclusions: The right spiral direction of the flossing band tended to increase the elasticity of the muscles compared to the left spiral direction. Therefore, in future studies, it is necessary to choose the direction of the flossing band to increase the elasticity of the muscles.

Design and Implementation of Business Flow Management System for XML/EDI based Electronic Bidding System (XML/EDI기반 전자입찰 시스템을 위한 비즈니스 흐름 관리기의 설계 및 구현)

  • 윤선희
    • Journal of the Korea Computer Industry Society
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    • v.4 no.10
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    • pp.751-760
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    • 2003
  • One of the business in the future will be executed by Business to Business(B to B) model of E-Commerce. The concept of the B to B model composes the work process, people, and supporting environment. The work processes are related to the information process, technologies, and standards. Information process elements contains digital data exchange, data sharing, and collaboration. This paper designed and implemented the XML-based Business Flow Management System(BFMS) for B to B model of E-commerce in the Internet. BFMS in this paper automatically performs the information process for B to B model such as electronic bidding system including purchase order management, bidding management, delivery management to build E-Commerce in the Internet. XML can be used as document standard between business for skating and exchange of data and also provide platform independent environment.

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Solution State Structure of pB1, the Mimotopic Peptide of Apolipoprotein B-100, by NMR

  • Lee, Sung-Ran;Kim, Dae-Sung;Kim, Hyo-Joon;Lee, Yong-Woo;Won, Ho-Shik
    • Bulletin of the Korean Chemical Society
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    • v.25 no.12
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    • pp.1845-1849
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    • 2004
  • Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 ${\AA}^2$ and the total RMSD was 0.5-1.5 ${\AA}. Moreover, pB1 structure included a weak $3_{10}$-helix from $Ile^7$,/TEX> to $Trp^{13}$.

DNMT3b Promoter Polymorphism and Risk of Gastric Cancer in the Korean Population (한국인에서 DNMT3b 유전자 다형성과 위암의 감수성)

  • Kim, Sung Geun;Jung, Hun;Kim, Sin Sun;Jeon, Kyung Hwa;Song, Kyo Young;Kim, Jin Jo;Jin, Hyung Min;Kim, Wook;Park, Cho Hyun;Park, Seung Man;Lim, Keun Woo;Kim, Seung Nam;Jeon, Hae Myung
    • Journal of Gastric Cancer
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    • v.7 no.1
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    • pp.9-15
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    • 2007
  • Purpose: DNA methylation is an important epigenetic factor in tumorigenesis. We hypothesized that polymorphism of the promoter of the DNA methyltransferase 3b (DNMT3b) genes, which are responsible for regulating the methylation status of tumor suppressor genes, are associated with increased risk of gastric cancer. Materials and Methods: In this hospital-based case-control study, to determine the role of this polymorphism of the promoter of DNA methyltransferase 3b (DNMT3b) genes in gastric cancer, we genotyped 176 cases and 70 control subjects. To determine the genotype, we used a polymerase chain reaction restriction fragment length polymorphism assay. We compared alleles and genotypes between the two groups and revealed an association of DNMT3b promoter polymorphism with increased risk of gastric cancer in the Korean population. Results: Genotype frequencies were 14.8% (Cytosine-Cytosine), 71.6% (Cytosine-Thymine), and 13.6% (Thymine- Thymine) in the case patients and 40.0% (Cytosine-Cytosine), 42.9% (Cytosine-Thymine), and 17.1% (Thymine-Thymine) in the control subjects, respectively. Compared with CC homozygotes, CT heterozygotes had a 4.523-fold increased risk (OR, 2.13; 95% CI, 2.324~8.803), and the TT homozygotes had a 2.154-fold elevated risk (OR, 1.42; 95% CI, 0.899~85.165). For the T variant genotype (CT+TT), there was a 3.846-fold increased risk (OR, 1.88; 95% CI, 2.040~7.251). However, no significance was observed in the genotype distributions of both polymorphisms according to histopathology, stage of stomach cancer. The Ssame results were observed with Helicobacter infection. Conclusion: DNMT3b promoter polymorphism, especially the T variant genotype, is associated significantly with thean increased risk of gastric cancer.

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Detection of Mendelian and Parent-of-origin Quantitative Trait Loci for Meat Quality in a Cross between Korean Native Pig and Landrace

  • Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, T.H.;Kim, K.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1644-1650
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) affecting meat quality in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 17 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between models were used to characterize the QTL for mode of gene expressions. A total of 10 (32) QTL were detected at the 5% genome (chromosome)-wise level for the analyzed traits. Of the 42 QTL detected, 13 QTL were classified as Mendelian, 10 as paternal, 14 as maternal, and 5 as partial expressed QTL, respectively. Among the QTL detected at 5% genome-wise level, four QTL had Mendelian mode of inheritance on SSCs 5, 10, 12, and 13 for cooking loss, drip loss, crude lipid and crude protein, respectively; two QTL maternal inheritance for pH at 24-h and shear force on SSC11; three QTL paternal inheritance for CIE b and Hunter b on SSC9 and for cooking loss on SSC15; and one QTL partial expression for crude ash on SSC13, respectively. Most of the Mendelian QTL (9 of 13) had a dominant mode of gene action, suggesting potential utilization of heterosis for genetic improvement of meat quality within the cross population via marker-assisted selection.