• 생명과학회지
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• 제25권8호
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• pp.889-895
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• 2015

혈관내피세포 기능장애는 동맥경화증 발생의 초기단계이다. 비타민B군(B6, B12, 엽산)은 혈관항상성에 중요한 인자이다. 이들 비타민B군이 결핍되면 혈관항상성에 변화가 생겨 심혈관질환을 유발한다. 비타민B군 중 엽산은 내피세포에서 산화질소 항상성에 중요한 역할을 한다. 동맥경화증에 관련된 섭취엽산의 용량의존적 효과를 알기 위해, C57BL/6 쥐의 대동맥이완과 간 C반응단백질 수준을 연구하였다. 본 연구는 총 54마리의 C57BL/6 쥐를 서양식이군과 대조식이군으로 나누고, 각각은 다시 엽산섭취용량(0.2, 2, 8mg/kg)에 따라 3형태의 하위집단으로 나누었다. 18개월 동안의 식이섭취후, 양군의 8mg/kg 엽산섭취용량에서는 대동맥 이완반응을 전혀 나타내지 않았고 양군의 차이도 없었다(p<0.05). 모든 엽산섭취용량(0.2, 2, 8mg/kg)에서 간 C반응단백질 수준은 대조식이군보다 서양식이군에서 더 높았다(p=0.035). 양군의 간 C반응단백질 수준은 0.2 mg/kg 엽산섭취용량에서 가장 낮았다(p<0.05). 결론적으로, C57BL/6 쥐에서 식이에 관계없이 0.2 mg/kg 엽산섭취용량만으로도 대동맥의 이완반응을 유도하고 간 C반응단백질 수준을 낮춤으로써 동맥경화증을 충분히 예방할 수 있다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.103-106
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• 2002

A gradient LC/MS system was constructed and applied for separation of biological samples. For example, a rapid and simple analytical method without pretreatment based on gradient ${\mu}LC/MS$ with a disposable microcolumn has been developed to determine B group vitamins in urine. Urine samples were directly injected to the disposable home-made microcolumn. The microcolumn can be emptied after being used for a series of urine samples, and repacked with fresh stationary phase. An overdose of vitamin pills were swallowed by healthy volunteers and the urine samples were taken 1,2,3,5, and 8 hours after swallowing. Vitamins immediately showed up in urine, hit the maximum, and disappeared swiftly. This technique is expected to have some application for clinical purposes.

• 한국식품영양과학회지
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• 제24권1호
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• pp.169-182
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• 1995

The nutritional evaluation, and antimutagenic and anticancer effects of Kimchi were reviewed. Kimchi contains high levels of vitamins including vitamin C, $\beta$-carotene, vitamin B complex, niacin, and of minerals such as calcium, potassium, iron and phosphorous, etc. Kimchi is a low energy food, byt contains high quantities oforganic acids, dietary fiber and lactic acid bacteria in addition to the vitamins and minerals. Thus Kimchi could be developed as a protective food as it contains the various regulatory nutrients. The levels of NO3, NO2 and nitrosamines in Kimchi ingredients and Kimchi during theripening are not significant. However, high level of NaCl that could be used when prepared Kimchi in the warm region can be a problem, since high concentraitons of NaCl(>9.5%) in Kimchi could be comutagenic to themutagen of MNNG. The methanol extract of Kimchi, red pepper powder, garlic and lactic bacteria revealed antimutagenic or anticancer activities. The Kimchi extract also inhibited the growth of various human cancer cells.

• Nutrition Research and Practice
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• 제2권1호
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• pp.35-40
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• 2008

A cross-sectional study involving 400 mothers was conducted in the Manya Krobo district of Ghana with the objective of studying household dietary practices, quality of diets and family nutritional status of rural Ghana, A combination of methods, including structured interviews using questionnaire, dietary assessments and anthropometry was used to collect data for the study. The data obtained was analyzed using Statistical Package for Social Sciences (SPSS) Version 10 in Windows. Means and standard deviations were generated for continuous variables and frequency distribution for categorical variables. Most women consumed meals three times a day but only a few (12.5%) cooked all three meals at home. Breakfast and lunch were the two main meals purchased from food vendors. The most frequently consumed food items on daily basis were the starchy staples, maize, fish, pepper, onion, tomato and palm fruits. The nutritional qualities of diets were poor in terms of calcium and the B-vitamins. A significant proportion of the women were nutritionally at risk of being either underweight (12%), overweight (17%) or obese (5%). For adequate nutrition in this population, nutrition education intervention programs aimed at improving nutrient intake through improved diet diversity and increased use of local foods rich in calcium and the B-vitamins needs to be undertaken. There is also the need to intensify education on excessive weight gain and its attendant health problems in the area.

• Journal of Nutrition and Health
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• 제38권3호
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• pp.211-218
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• 2005

Elevated homocysteine concentration is known to be related to placental abruption, spontaneous abortion, and many adverse pregnancy outcomes. The purpose of this study was to investigate the effects of folic acid supplementation ($1000{\cal}ug$ per day) and 5, 10 methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism genotype on serum homocysteine and B vitamin levels in 50 infertile women ($31.2{\pm}3.2\;years$). Blood sampling was performed at baseline and at the end of folic acid supplementation period. In infertile women, serum folate and vitamin $B_{12}$ concentrations were significantly higher in post-supplementation than those in pre-supplementation. Serum homocysteine concentration was significantly lower in post-supplementation than that in pre-supplementation. However, serum homocysteine levels were still higher in the T/T genotype than those in the C/C or C/T even after folic acid supplementation. Serum homo-cysteine was inversely related to serum folate in T/T homozygotes at baseline and at the end of folic acid supplementation. These results suggest that folic acid supplementation is needed for infertile women to improve their vitamin status and also to reduce the risk of hyperhomocysteinemia. These effects were different according to their MTHFR C677T genotypes. Therefore, further studies are necessary to determine the optimal level of supplementation of folic acid by MTHFR genotypes.

• Journal of Nutrition and Health
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• 제36권4호
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• pp.389-396
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• 2003

Maternal nutritional status has been shown to influence pregnancy outcomes. And the elevated maternal plasma homocysteine concentrations have been associated with adverse pregnancy outcomes. We investigated the effects of maternal serum levels of B vitamins and homocysteine, and the C677T MTHFR (5, 10-methylenetetrahydrofolate reductase) polymorphism on pregnancy outcomes. In 177 pregnant women of 24-28 wks of gestation, the MTHFR gene mutation, serum B vitamins and homocysteine concentrations were measured, and their pregnancy outcomes were investigated from medical records. The birth length, and 1- and 5-min Apgar scores of neonates in the T/T mothers were 45.4 $\pm$ 9.3 cm, 7.6 $\pm$ 3.2 and 8.5 $\pm$ 3.8, respectively, which were significantly lower than those in the C/T (48.6 $\pm$ 3.3 cm, 9.0 $\pm$ 0.2, 10.0 $\pm$ 0.2) or the C/C mothers (49.4 $\pm$ 1.9 cm, 9.0 $\pm$ 0.2, 10.0 $\pm$ 0.0). The birth weight, birth length and the gestational age of neonates at delivery from hyperhomocysteinemic mothers whose homocysteine levels higher than 15 $\mu$ mol were 2.5 $\pm$ 1.3 kg, 43.9 $\pm$ 9.0 cm, 35.4 $\pm$ 6.3 wk, respectively, which were significant lower than those from normohomocysteinemic mothers (3.1 $\pm$ 0.6 kg, 48.8 $\pm$ 3.6 cm, 38.5 $\pm$ 2.5 wk). The birth weight and birth length of neonates in mothers whose PLP levels were below the median were significantly lower than those from mothers with the PLP levels above the median. The 1- and 5-min Apgar scores of neonates were lower in mothers with the T/T MTHFR genotype than those with the C/T or C/C only when the serum PLP levels were below the median. The 1-, 5 min Apgar scores and birth length of neonates were lower in mothers with the T/T MTHFR genotype than those with the C/T or C/C only when the serum FMN levels were below the median. In conclusion, maternal B vitamin status, homocysteine and the C677T MTHFR genotype seem to have played an important role on pregnancy outcomes.

• 생명과학회지
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• 제24권12호
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• pp.1301-1307
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• 2014

지방유래줄기세포(adipose-derived stem cell; ADSC)는 조직재생을 위한 탁월한 수단으로 인정되고 있으나 세포증식속도가 느려 ADSC의 배양용 배지에는 대게 fetal bovine serum (FBS)이 첨가된다. FBS는 세포에 다양한 영양분을 공급하지만 세포의 기능에 영향을 미칠 수 있는 미 동정 물질도 많이 함유하고 있다. FBS에 의한 예상밖의 영향과 동물유래물질의 오염을 방지하기 위해 ADSC의 무혈청 배양법에 관한 연구가 광범위하게 이루어지고 있다. 본 연구에서는 ADSC세포에 SV40의 T항원 유전자를 도입하여 증식속도를 향상시킨 ADSC-T세포주의 효율적인 무혈청 배양법을 확립하기 위해 ADSC-T의 세포증식에 미치는 아미노산복합체, 비타민 복합체 및 여러가지 영양분 혼합물(B27)의 영향을 검토하였다. 그 결과, ADSC-T세포를 DMEM/F12 무혈청 배지에 현탁하여 plate에 주입하였을 때는 증식하지 않았으며 아미노산, 비타민 및 B27 영양소복합체는 증식촉진효과를 나타내지 않았다. 그러나 ADSC-T세포를 유혈청 DMEM배지로 24시간 배양 후 DMEM/F12 무혈청 배지로 교체하여 배양했을 때는 세포가 증식하였으며 이때, 비타민 복합체와 B27 영양소복합체는 증식촉진효과를 나타내었다. 또한 Stem pro 배지를 이용하면 ADSC-T의 무혈청 부유배양이 가능한 것으로 나타났다. ADSC-T세포는 분자량 70 kDa 부근의 단백질을 다량으로 분비하였으며, 성장인자 중에서 insulin-like growth factor (IGF)와 fibroblast growth factor basic (FGF basic)는 유혈청 배양보다 무혈청 배양에서 더 많이 분비되었다.

• 생명과학회지
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• 제17권12호
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• pp.1689-1694
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• 2007

시판되고 있는 성장단계별로 10종의 영아용 조제분유(0-5개월용, 6-12개월용)의 수용성 및 지용성 비타민 함량을 표시된 첨가수준을 이용하여 비교 분석하였다. 조제분유 100 g 및 100 kcal 내 비타민 함량을 제품별로 분석하고 한국인 영양섭취기준에 의거하여 적합성을 고찰한 결과는 다음과 같다. 분말형 조제유 100 g당 각 비타민 함량은 전반적으로 조제분유 기준에 충족됨을 알 수 있었다. 100 kcal를 기준으로 영양소 적정량을 제시한 Codex 규격과 비교 분석한 결과 역시 모든 비타민이 Codex 기준 함량을 모두 충족하였다. 영아의 에너지 필요추정량을 기준으로 한국인 영양섭취기준과 비교, 분석한 결과, 본 실험에 사용한 고형분 조제분유의 비타민 함량이 섭취기준의 충분섭취량을 충족함을 알 수 있었다. 0-5개월용 고형분 조제분유의 경우, 지용성 비타민 A, D, E, K는 각각 영양섭취기준 충분섭취량의 약 178.6%, 205.3%, 208.4%, 976.3%를 함유하였으며, 수용성 비타민의 경우, 비타민 C, 티아민, 리보플라빈, 니아신, $B_6$, 엽산, $B_{12}$, 판토텐산 및 비오틴은 각각 충분섭취 량의 약 173.15%, 237.22%, 269.75%, 295.9%, 431.55%, 165.75%, 1186.25%, 203.77%, 408.33%를 함유하고 있다. 6-11개월용 고형분 조제분유의 경우, 지용성 비타민 A, D, E, K는 각각 영양섭취기준의 충분섭취량의 약 199.2%, 262.3%, 220.5%, 626.46%를 함유하였으며, 비타민 C, 티아민, 리보플라빈, 니아신, $B_6$, 엽산, $B_{12}$, 판토텐산 및 비오틴은 각각 충분섭취량의 약 179.46%, 210.18%, 264.65%, 241.54%, 205.95%, 166.86%, 699.54%, 246.95%, 475.03%로 함유됨을 알 수 있었다.

• 대한치위생과학회지
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• 제4권1호
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• pp.39-51
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• 2021

Background: Limited information is available regarding the associations of various nutrients and periodontitis in Korea. Furthermore, these associations have been controversial in previous studies. Therefore, the present study aimed to evaluate the associations between several nutrients and periodontitis. Methods: Pooled data from the 2007~2010 and 2012 Korea National Health and Nutrition Examination Surveys (KNHANES) were used for the present study. Periodontitis was defined using the World Health Organization (WHO) Community Periodontal Index. All participants completed the Food Frequency Questionnaire (FFQ), which was analyzed using CAN-Pro 4.0. Vitamins A, B₁, B₂, and C, iron, phosphorus, calcium, and protein were selected for analysis. Multiple logistic regression analyses were used to estimate the odds ratios (ORs) for periodontitis. Results: Lower intake of phosphorus (adjusted odds ratio [AOR] = 1.86), calcium (AOR = 1.25), and protein (AOR = 1.52) were associated with periodontitis in males, and lower intake of vitamins A (AOR = 1.32), B1 (AOR = 1.21), B2 (AOR = 1.24), and C (AOR = 1.21), iron (AOR = 1.25), phosphorus (AOR = 1.33), and protein (AOR = 1.27) showed significant relationships with periodontitis in females. In the sociodemographic, somatic, and health behavior-adjusted models, lower phosphorus (AOR = 2.68) and protein (AOR = 1.68) intake in younger males, and lower vitamin A intake (AOR = 1.37) in middle-aged females were significantly associated with periodontitis. Conclusions: The results of the present study demonstrated a significant association between periodontitis and nutrient intake. To avoid an insufficient nutrient supply for a patient with periodontal disease, the patient's diet should be closely monitored.

• 한국식생활문화학회지
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• 제23권2호
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• pp.258-267
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• 2008

The purpose of this study was to investigate the effects that body composition, nutrient intake, and biochemical indices have on skin status, as well as their associations with skin health status, by assessing 44 female university students. The subjects were classified into 3 groups according to skin sensitivity: sensitive skin (n=11), slightly sensitive skin (n=24), and normal skin (n=9). The study included anthropometric measurements and body composition analyses, dietary intake evaluations, blood chemical analyses, and skin health status assessments. Based on % body fat, the results indicated the subjects were moderately obese (29.6%). Obesity indices were not different among the groups, but the sensitive skin group tended to have higher skinfold thickness. Dry skin was determined as the skin type of all subjects. In the sensitive skin groups, U-zone and T-zone sebum were significantly lower (p<0.05), and spots were significantly higher (p<0.05) than in the normal skin group. In terms of nutrient intakes, the sensitive skin groups consumed significantly lower amounts of P and vitamins A and E (p<0.05). There were no significant differences in serum lipid fractions or total antioxidant status among the groups. In correlation coefficients analysis, skin elasticity had significant negative correlations with subscapular and midaxillary skinfold thickness, body protein, and body minerals (p<0.05). Wrinkling had positive correlations with triceps and thigh skinfold thickness (p<0.05). Skin evenness had negative correlations with energy intake, vitamins A, $B_1,\;B_2$, C, E, niacin, and zinc (p<0.05), as well as folate and P (p<0.01) and vitamin $B_6$ (p<0.001). A similar correlation tendency was observed for spots and nutrient intake, showing negative correlations with vitamins A, $B_6$, and E, as well as folate, Fe, and P. U-zone sebum showed significant positive correlations with serum levels of total cholesterol and triglycerides. From these findings, skin health status appears to be affected by both nutrient intake and body composition. Therefore, having a proper balance between dietary intake and body composition may influence skin health status in females with sensitive skin.