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Effects of variety, region and season on near infrared reflectance spectroscopic analysis of quality parameters in red wine grapes

  • Esler, Michael B.;Gishen, Mark;Francis, I.Leigh;Dambergs, Robert G.;Kambouris, Ambrosias;Cynkar, Wies U.;Boehm, David R.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1523-1523
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    • 2001
  • The wine industry requires practical methods for objectively measuring the composition of both red wine grapes on the vine to determine optimal harvest time; and of freshly harvested grapes for efficient allocation to vinery process streams for particular red wine products, and to determine payment of contract grapegrowers. To be practical for industry application these methods must be rapid, inexpensive and accurate. In most cases this restricts the analyses available to measurement of TSS (total soluble solids, predominantly sugars) by refractometry and pH by electropotentiometry. These two parameters, however, do not provide a comprehensive compositional characterization for the purpose of winemaking. The concentration of anthocyanin pigment in red wine grapes is an accepted indicator of potential wine quality and price. However, routine analysis for total anthocyanins is not considered as a practical option by the wider wine industry because of the high cost and slow turnaround time of this multi-step wet chemical laboratory analysis. Recent work by this ${group}^{l,2}$ has established the capability of near infrared (NIR) spectroscopy to provide rapid, accurate and simultaneous measurement of total anthocyanins, TSS and pH in red wine grapes. The analyses may be carried out equally well using either research grade scanning spectrometers or much simpler reduced spectral range portable diode-array based instrumentation. We have recently expanded on this work by collecting thousands of red wine grape samples in Australia. The sample set spans two vintages (1999 and 2000), five distinct geographical winegrowing regions and three main red wine grape varieties used in Australia (Cabernet Sauvignon, Shiraz and Merlot). Homogenized grape samples were scanned in diffuse reflectance mode on a FOSE NIR Systems6500 spectrometer and subject to laboratory analysis by the traditional methods for total anthocyanins, TSS and pH. We report here an analysis of the correlations between the NIR spectra and the laboratory data using standard chemometric algorithms within The Unscrambler software package. In particular, various subsets of the total data set are considered in turn to elucidate the effects of vintage, geographical area and grape variety on the measurement of grape composition by NIR spectroscopy. The relative ability of discrete calibrations to predict within and across these differences is considered. The results are then used to propose an optimal calibration strategy for red wine grape analysis.

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Wine quality grading by near infrared spectroscopy.

  • Dambergs, Robert G.;Kambouris, Ambrosias;Schumacher, Nathan;Francis, I. Leigh;Esler, Michael B.;Gishen, Mark
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1253-1253
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    • 2001
  • The ability to accurately assess wine quality is important during the wine making process, particularly when allocating batches of wines to styles determined by consumer requirements. Grape payments are often determined by the quality category of the wine that is produced from them. Wine quality, in terms of sensory characteristics, is normally a subjective measure, performed by experienced winemakers, wine competition judges or winetasting panellists. By nature, such assessments can be biased by individual preferences and may be subject to day-to-day variation. Taste and aroma compounds are often present in concentrations below the detection limit of near infrared (NIR) spectroscopy but the more abundant organic compounds offer potential for objective quality grading by this technique. Samples were drawn from one of Australia's major wine shows and from BRL Hardy's post-vintage wine quality allocation tastings. The samples were scanned in transmission mode with a FOSS NIR Systems 6500, over the wavelength range 400-2500 ㎚. Data analysis was performed with the Vision chemometrics package. With samples from the allocation tastings, the best correlations between NIR spectra and tasting data were obtained with dry red wines. These calibrations used loadings in the wavelengths related to anthocyanins, ethanol and possibly tannins. Anthocyanins are a group of compounds responsible for colour in red wines - restricting the wavelengths to those relating to anthocyanins produced calibrations of similar accuracy to those using the full wavelength range. This was particularly marked with Merlot, a variety that tends to have relatively lower anthocyanin levels than Cabernet Sauvignon and Shiraz. For dry white wines, calibrations appeared to be more dependent on ethanol characteristics of the spectrum, implying that quality correlated with fruit maturity. The correlations between NIR spectra and sensory data obtained using the wine show samples were less significant in general. This may be related to the fact that within most classes in the show, the samples may span vintages, glowing areas and winemaking styles, even though they may be made from only one grape variety. For dry red wines, the best calibrations were obtained with a class of Pinot Noir - a variety that tends to be produced in limited areas in Australia and would represent the least matrix variation. Good correlations were obtained with a tawny port class - these wines are sweet, fortified wines, that are aged for long periods in wooden barrels. During the ageing process Maillard browning compounds are formed and the water is lost through the barrels in preference to ethanol, producing “concentrated” darkly coloured wines with high alcohol content. These calibrations indicated heaviest loadings in the water regions of the spectrum, suggesting that “concentration” of the wines was important, whilst the visible and alcohol regions of the spectrum also featured as important factors. NIR calibrations based on sensory scores will always be difficult to obtain due to variation between individual winetasters. Nevertheless, these results warrant further investigation and may provide valuable Insight into the main parameters affecting wine quality.

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Simultaneous Analytical Method for the Neomycin, Gentamicin Residues in Seafood (수산물 중 네오마이신, 겐타마이신 동시분석법 개발)

  • Hong, Young-Min;Lee, Seok-Ki;Kim, Hyoung-Ah;Hwang, Yu-Kyung
    • Journal of Applied Biological Chemistry
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    • v.53 no.1
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    • pp.25-30
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    • 2010
  • This paper describes a simultaneous method for the determination of two aminoglycosides (neomycin and gentamicin) using solid phase extraction followed by liquid chromatograph-mass spectrometry. The extract was applied to an WCX and HLB solid phase extraction cartridge. The cartridges were washed with water and methanol, and analytes were eluted with TCA buffer-acetonitrile mixture. The aminoglycosides were separated by ion-pairing reversed phase mode prior to ESI-LC/MS. Under the conditions applied neomycin was almost separated from all the gentamicin compounds. No interfering peaks from endogenous compounds of matrix were noted at the elution position of the analytes. Recoveries of neomycin fortified at levels of 0.25, 0.5, 1.0 and 2.0 mg/kg seafood samples ranged from 92 to 115%. Recoveries of gentamycin fortified at levels of 0.05, 0.1, 0.2, 0.4 mg/kg seafood samples ranged from 99 to 116%. Method detection limits in four seafood sample matrices were between 0.002 and 0.033 mg/kg.

Effects of Infrared Pasteurization on Quality of Red Pepper Powder (적외선 살균이 고춧가루의 품질에 미치는 영향)

  • Jung, Jin-Joo;Choi, Eun-Ju;Lee, You-Jin;Kang, Sung-Tae
    • Korean Journal of Food Science and Technology
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    • v.43 no.2
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    • pp.156-160
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    • 2011
  • A tray type-infrared (IR) pasteurization system was developed for decreasing microorganisms in red pepper powder (RPP). The RPP was passed through a tray by a vibrating mode under the 4 IR lamps (total 8000 W) and by circulating water under the tray. Fungi was pasteurized by applying power higher than 2000 W to the RPP. The decrease in viable cell numbers of bacteria, however, was not observed under the same conditions. Conveying speed of RPP was optimized to 106-164 g/min on the basis of microbial reduction and retaining of moisture content of RPP. The water content of 32 mesh-RPP decreased rapidly after pasteurization. However, fungi in both RPPs could be sterilized regardless of particle sizes. The repetition of IR pasteurization was not favourable due to severe decrease of water content in RPP. The IR pasteurization of RPP did not cause significant difference in the capsaicinoid contents, ASTA colour value, and L, a, and b values under all investigated conditions.

High-$T_c$ 2nd-order SQUID Gradiometer for Use in Unshielded Environments (비차폐 환경에서의 고온초전도 SQUID 2차 미분기의 특성연구)

  • 박승문;강찬석;이순걸;유권규;김인선;박용기
    • Progress in Superconductivity
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    • v.5 no.1
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    • pp.50-54
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    • 2003
  • We have fabricated $∂^2$$B_{z}$ /$∂x^2$ type planar gradiometers and studied their properties in operation under various field conditions. $YBa_2$$Cu_3$$O_{7}$ film was deposited on $SrTiO_3$ (100) substrate by a pulsed laser deposition (PLD) system and patterned into a device by the photolithography with ion milling technique. The device consists of 3 pickup loops designed symmetrically Inner dimension and the width of the square side loops are 3.6 mm and 1.2 mm, respectively, and the corresponding dimensions of the center loop are 2.0 mm and 1.13 mm. The length of baseline gradiometer is 5.8 mm. Step-edge junction width is 3.0 $\mu\textrm{m}$ and the hole size of the SQUID loop is 3 $\mu\textrm{m}$ ${\times}$ 52 $\mu\textrm{m}$. The SQUID inductance is estimated to be 35 pH. The device was formed on a 20 mm ${\times}$ 10 mm substrate. We have tested the behavior of the device in various field conditions. The unshielded gradiometer was stable under extremely hostile conditions on a laboratory bench. Noise level 0.45 pT/$\textrm{cm}^2$/(equation omitted)Hz and 0.84 pT/$\textrm{cm}^2$/(equation omitted)Hz at 1 Hz for the shielded and the unshielded cases, which correspond to equivalent field noises of 150 fT/(equation omitted)Hz and 280 fT/(equation omitted)Hz, respectively. In spite of the short baseline of 5.8 mm, the high common-mode-rejection-ratio of the gradiometer, $10^3$, allowed us to successfully record magnetocardiogram of a human subject, which demonstrates the feasibility of the design in biomagnetic studies.

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Estimation of Genetic Parameters on Pre-weaning Growth Traits in Hanwoo (한우의 이유전 성장형질에 관한 유전모수 추정)

  • Hwang, J.M.;Choi, J.G.;Kim, H.C.;Choy, Y.H.;Lee, C.;Yang, B.K.;Shin, J.S.;Kim, Jong-Bok
    • Journal of Animal Science and Technology
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    • v.49 no.2
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    • pp.171-176
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    • 2007
  • A total of 4,627 records on pre-weaning growth traits, birth weight(BW), weaning weight(WW) and pre-weaning daily gain(ADG) of Hanwoo’s born form 1970 to 2005 were collected from Hanwoo Experiment Station, National Livestock Research Institute. Variance components and heritabilities of BW, WW and ADG were estimated with two models; Model 1 included only direct genetic effect while Mode1 2 included direct genetic, maternal genetic and permanent environmental effects. Direct herit- ability estimates of BW, WW and ADG estimated by model 1 were 0.34, 0.26 and 0.20, respectively. In model 2, direct heritability and maternal genetic heritability estimates were 0.14 and 0.06 for BW, 0.07 and 0.04 for WW and 0.05 and 0.04 for ADG. Estimates of genetic correlation between direct and maternal effect for BW, WW and ADG were 0.58, 0.75 and 0.61, respectively. Genetic correlation coefficients between BW and WW, between BW and ADG, and between WW and ADG were 0.76, 0.62 and 0.99, respectively.

Characterization of angiotensin II antagonism displayed by KR-31081, a novel nonpeptide AT1 receptor antagonist (안지오텐신 수용체 길항제 KR-31081의 특성에 관한 연구)

  • Lee, Sung-Hou
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.2997-3003
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    • 2009
  • The pharmacological profile of KR-31081, a nonpeptide $AT_1$ selective angiotensin receptor antagonist, was investigated by receptor binding studies, functional in vitro assays with rabbit aorta. KR-31081 inhibited the specific binding of $[^{125}I]\;[Sar^1,\;Ile^8]$-angiotensin II to human recombinant $AT_1$ receptor with an 8.6-fold greater potency than losartan ($IC_{50}$: 1.43 and 12.3 nM, respectively), but it did not inhibit the binding of [$^{125}I$] CGP 42112A to human recombinant $AT_2$ receptor ($IC_{50}$: higher than $10{\mu}M$ for both). The Hill coefficient for the competition curve of KR-31081 against $AT_1$ receptor was not significantly different from unity (0.99). Scatchard analysis showed that KR-31081 interacted with human recombinant $AT_1$ receptor in a competitive manner, as with losartan. In functional studies with rabbit aorta, KR-31081 competitively inhibited the contractile response to angiotensin II ($pK_B$ values: 8.66) with 20-70% decrease in the maximum contractile responses, unlike losartan that showed competitive antagonism without any change in the maximum contractile responses to angiotensin II ($pA_2$ values: 7.59). These results suggest that KR-31081 is a highly potent $AT_1$ selective angiotensin II receptor antagonist with a mode of insurmountable antagonism to be developed as the exploratory potential of this compound.

Ocean Circulation Model ing of East Sea for Aquatic Dispersion of Liquid Radioactive Effluents from Nuclear Power Plants (원전 액체 방사성 유출물 해양확산 평가를 위한 동해 해수순환 모델링)

  • Chung Yang-Geun;Lee Gab-Bock;Bang Sun-Young;Lee Ung-Gwon;Lee Yong-Sun
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.11a
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    • pp.321-331
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    • 2005
  • Recently. three-dimensional models have been used for aquatic dispersion of radioactive effluents in relation to nuclear power plant siting based on the Notice No. 2003-12 'Guideline for investigating and assessing hydrological and aquatic characteristics of nuclear facility site' of the Ministry of Science and Technology (MOST) in Korea. Several nuclear power plants have been under construction or planed. which are Shin-Korl Unit 1 and 2, Shin-Wolsong Unit 1 and 2, and Shln-Ulchin Unit 1 and 2. For assessing the aquatic dispersion of radionuclides released from the above nuclear power plants, it is necessary to know the coastal currents around sites which are affected by circulation of East Sea. In this study, a three dimensional hydrodynamic model for the circulation of the East Sea of Korea has been developed as the first Phase, which Is based on the RIAMOM. The model uses the primitive equation with hydrostatic approximation, and uses Arakawa-B grid system horizontally and Z-coordinate vertically. Model domain is $126.5^{\circ}E\;to\;142.5^{\circ}E$ of east longitude and $33^{\circ}N\;and\;52^{\circ}N$ of the north latitude. The space of the horizontal grid was $1/12^{\circ}$ to longitude and latitude direction and vortical level was divided to 20. This model uses Generalized Arakawa Scheme. Slant Advection, and Mode-Splitting Method. The input data were from JODC, KNFRDI, and ECMWF. The model ing results are in fairly good agreement with schematic patterns of the surface circulation in the East Sea The local current model and aquatic dispersion model of the coastal region will be developed as the second phase. The oceanic dispersion experiments will be also tarried out by using ARGO Drifter around a nuclear pelter plant site.

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Integrating Portable Internet with cdma2000 Mobile Communication Networks for Seamless Service (연속적인 서비스를 위한 휴대 인터넷과 cdma2000 이동통신망의 연동 방안)

  • Cho Jinsung;Kim Jeong Gem
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.11B
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    • pp.920-929
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    • 2004
  • Nowadays, wireless packet data services are provided over cdma2000 1x/1xEV-DO mobile communication network and Portable Internet are being standardized for users demanding higher data rate services. Portable Internet can provide high data rate services, but its service coverage is relatively small. If Portable Internet may be integrated with cdma2000 mobile networks, users are able to choose the best service according to service areas and get seamless services while they are moving around. At the same time, it is cost-effective for operators to construct and maintain the integrated network. For the purpose of effectively integrating Portable Internet into cdma2000 networks, we propose an integration scheme including network architecture, protocol architecture, functions in network elements, interfaces between them, and call-flow procedures. The integration scheme proposed in this paper adopts a tightly-coupled architecture for unified authentication/accounting and seamless services. In addition, the scheme can be implemented without modifying the existing cdma2000 mobile communication networks. It is also simple to develop the dual-mode mobile station. Through the simulation results based on the performance model for handoffs between cdma2000 and Portable Internet, it has teen validated that the proposed scheme diminishes packet losses compared with the loosely-coupled architecture.

Fault free Shortest Path routing on the de Bruijin network (드브르젼 네트워크에서 고장 노드를 포함하지 않는 최단 경로 라우팅)

  • Ngoc Nguyen Chi;Nhat Vo Dinh Minh;Zhung Yonil;Lee Sungyoung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.11B
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    • pp.946-955
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    • 2004
  • It is shown that the do Bruijn graph (dBG) can be used as an architecture for interconnection network and a suitable structure for parallel computation. Recent works have classified dBG based routing algorithms into shortest path routing and fault tolerant routing but investigation into fault free shortest path (FFSP) on dBG has been non-existent. In addition, as the size of the network increase, more faults are to be expected and therefore shortest path dBG algorithms in fault free mode may not be suitable routing algorithms for real interconnection networks, which contain several failures. Furthermore, long fault free path may lead to high traffic, high delay time and low throughput. In this paper we investigate routing algorithms in the condition of existing failure, based on the Bidirectional do Bruijn graph (BdBG). Two FFSP routing algorithms are proposed. Then, the performances of the two algorithms are analyzed in terms of mean path lengths and discrete set mean sizes. Our study shows that the proposed algorithms can be one of the candidates for routing in real interconnection networks based on dBG.